서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Cell proliferation and neuroblast differentiation in the rat dentate gyrus after intrathecal treatment with adipose-derived mesenchymal stem cells.

Choi, Jung Hoon,Chung, Jin Young,Yoo, Dae Young,Hwang, In Koo,Yoo, Ki-Yeon,Lee, Choong Hyun,Yan, Bing Chun,Ahn, Jin Ok,Youn, Hwa Young,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2011 Cellular and molecular neurobiology Vol.31 No.8

<P>Mesenchymal stem cells (MSC) have emerged as a new therapeutic tool for a number of clinical applications, because they have multipotency and paracrine effects via various factors. In the present study, we investigated the effects of adipose-derived MSC (Ad-MSC) transplantation via intrathecal injection through the cisterna magna on cell proliferation and differentiation of endogenous stem cells in the hippocampal dentate gyrus (DG) using Ki-67 (a marker for proliferating cells), and doublecortin (DCX, a marker for neuroblasts). The transplanted Ad-MSC were detected in the meninges, not in the hippocampal parenchyma. However, the number of Ki-67-immunoreactive cells was significantly increased by 83% in the DG 2 days after single Ad-MSC injection, and by 67% at 23 days after repeated Ad-MSC treatment compared with that in the vehicle-treated group after Ad-MSC transplantation. On the other hand, the number of DCX-immunoreactive cells in the DG was not changed at 2 days after single Ad-MSC injection; however, it was significantly increased by 62% 9 days after single Ad-MSC injection. At 23 days after repeated Ad-MSC application, the number of DCX-immunoreactive cells was much more increased (223% of the vehicle-treated group). At this time point, DCX protein levels were also significantly increased compared with those in the vehicle-treated group. These results suggest that the intrathecal injection of Ad-MSC could enhance endogenous cell proliferation, and the repeated Ad-MSC injection could be more efficient for an enhancement of endogenous cell proliferation and differentiation in the brain.</P>

Effects of pyridoxine on a high‐fat diet‐induced reduction of cell proliferation and neuroblast differentiation depend on cyclic adenosine monophosphate response element binding protein in the mouse dentate gyrus

Yoo, Dae Young,Kim, Woosuk,Yoo, Ki‐,Yeon,Nam, Sung Min,Chung, Jin Young,Yoon, Yeo Sung,Won, Moo‐,Ho,Hwang, In Koo Wiley Subscription Services, Inc., A Wiley Company 2012 JOURNAL OF NEUROSCIENCE RESEARCH - Vol.90 No.8

<P><B>Abstract</B></P><P>In this study, we challenged pyridoxine to mice fed a high‐fat diet (HFD) and investigated the effects of pyridoxine on HFD‐induced phenotypes such as blood glucose, reduction of cell proliferation and neuroblast differentiation in the dentate gyrus using Ki67 and doublecortin (DCX), respectively. Mice were fed a commercially available low‐fat diet (LFD) as control diet or HFD (60% fat) for 8 weeks. After 5 weeks of LFD or HFD treatment, 350 mg/kg pyridoxine was administered for 3 weeks. The administration of pyridoxine significantly decreased body weight in the HFD‐treated group. In addition, there were no significant differences in hepatic histology and pancreatic insulin‐immunoreactive (‐ir) and glucagon‐ir cells of the HFD‐treated group after pyridoxine treatment. In the HFD‐fed group, Ki67‐positive nuclei and DCX‐ir neuroblasts were significantly decreased in the dentate gyrus compared with those in the LFD‐fed mice. However, the administration of pyridoxine significantly increased Ki67‐positive nuclei and DCX‐ir neuroblasts in the dentate gyrus in both LFD‐ and HFD‐fed mice. In addition, the administration of pyridoxine significantly increased the protein levels of glutamic acid decarboxylase 67 (GAD67) and brain‐derived neurotrophic factor (BDNF) and the immunoreactivity of phosphorylated cyclic AMP response element binding protein (pCREB) compared with the vehicle‐treated LFD‐ and HFD‐fed mice. In contrast, the administration of pyridoxine significantly decreased HFD‐induced malondialdehyde (MDA) levels in the hippocampus. These results showed that pyridoxine supplement reduced the HFD‐induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus via controlling the levels of GAD67, pCREB, BDNF, and MDA. © 2012 Wiley Periodicals, Inc.</P>

Tetrahydroisoquinoline Alkaloids가 쥐의 동맥과 뇌균등질의 α-Adrenoceptors에 미치는 영향

이현석,백선하,황수현,박인성,김은상,정진명,한종우,장기철,장기철 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.1

Focal adhesion kinase and src expression in premalignant and malignant skin lesions

( Ju Yeon Choi ),( Jae Yun Lim ),( Han Saem Kim ),( Jung Min ),( Jung In Kim ),( Hyun Min Seo ),( Sang Hyeon Hwang ),( Chong Won Choi ),( Yoon Hwan Kim ),( Jin Hee Sohn ),( Hyunjoo Lee ),( Won Serk Ki 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: Focal adhesion kinase (FAK) and Src are non-receptor tyrosine kinases. FAK and Src play a critical role in inducing malignant transformation in tumor cells. Objectives: We performed immunohistochemical staining for total and phosphorylated forms of FAK and Src, to evaluate the role of FAK and Src in the development of premalignant and malignant skin lesions. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Methods: A total of 59 facial skin samples (30 actinic keratoses, 10 Bowen``s diseases, 13 squamous cell carcinomas and six perilesional skins) were immunohistochemically stained for Ki-67, total (t) and phosphorylated (p) form of FAK and Src. Results: Cells positive for t-Src, p-Src-y530, t-FAK and pFAK-s722 were detected in premalignant intra-epithelial lesions (PELs) and squamous cell carcinomas (SCCs), but not in the perilesional skin. There was a tendency towards high correlation between Ki-67 and t-FAK or pFAK-s722, suggestive of the active role of FAK in cell proliferation. Conclusion: Our findings of higher t-Src and p-Src-y530 positive cells in PELs, as compared to SCCs (with higher Ki-67 level), are suggestive of the other role of Src in tumor formation and progression, which requires further investigation.

성의 회복과 공존 : Beloved

황수진,이기한 명지대학교 인문과학연구소 2002 인문과학연구논총 Vol.24 No.-

As a first meaning step towards restoration of gender consciousness, this study seeks to investigate the modalities of racial discrimination and of sexual repression within the patriarchal system, as portrayed in Toni Morrison’s Beloved. It is the thesis of this study that the redefinition of motherhood through the painstaking ritual of rememory, as represented by Sethe and Beloved, ultimately opens new possibilities for harmonious coexistence between the two polar genders.

대수응력모형과 레이놀즈응력모형에 의한 표층밀도류장의 연구

황용진,김기홍 진주산업대학교 2000 산업과학기술연구소보 Vol.- No.7

The programs of two models(Reynolds Stress Model and Algebraic Stress Model) were developed and the distribution of mean velocity and buoyancy by RSM and ASM were computed to compare with experimental data in two-dimensional buoyant surface jet. The results of numerical analysis are derived : 1. RSM and ASM yield satisfactory prediction of distribution of mean velocity and buoyancy without regard to densimetric Froude number F_do in momentum domain region and transition region. 2. But, the computed results of distribution of mean velocity and buoyancy by two models are not in agreement with the experimental data correctly without regard to densimetric Froude number F_do in buoyancy domain region.

대학생의 운동과 스포츠 장애에 관한 연구

황향희,이상진,부기원 강원대학교 체육과학연구소 2000 江原大學校附設體育科學硏究所論文集 Vol.- No.23

The purpose of this study was to survey and analyze the response of college students on the barriers to exercise and sport with 1,000 students(female : 500, male :500) on the Chunchon city in Kangwon province were answered by the questionnaire. Analyse of the data with 732(female:374, male:358) for the evaluation of the questionnaire can be acquired the more basis materials of sports environment for the university students. As result, we have the conclusion as follow. 1.There are no difference(P> .05) in their sex and factor of sport barriers, but there is a different(P <.001) between the factors of sport barriers. 2.Their major and factor of sport barriers weren't a difference, but there is a difference<P <.01) between the factors of sport barriers. 3.Whether they was a member of exercise group or not and factor of sport barriers. There is no significant differences according to whether they was a member of exercise group or not level at P> .05, but there is a difference(P <.001) between the factors of sport barriers. 4.In their grade and factor of sport barriers, there is a meaningful difference level at P <.01, but there is no difference between the factors of sport barriers level at P <.001. Group of 2d, 3d, 4th more disturbed than average in order of barriers of resources, club, and program, but the reflection of 1st grade;s group in order to barriers of resources, program, club, and satisfaction at the program. Approximately, 1st grade's reflection seemed level at average. 5.As a result of the analysis of variance that their sex, major, whether they was a member of exercise group or not, grade and the factors of sport barriers. Generally, they were so many disturbed by barriers of resources, and then they showed strong rejection symptoms toward high price they should pay for using exercise facilities. The college student's exercise and sports activities weren't disturbed by their health. In addition, there is no significant difference in the effect of interaction that in their sex, major, whether they was a member of exercise group or not, grade and the factors of sport barriers.

말초혈액 내 백혈구수와 대사증후군과의 연관성

황상태,김범수,황상준,서정열,김병진,이원영,성기철,강진호,김선우,이만호,박정로 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

核置換에 의한 3n Populus alba×glandulosa의 育成 "in virto" 硏究

黃震聲,金鼎錫,尹基植 진주산업대학교 1985 論文集 Vol.23 No.-

생장이 속한 allotriploid의 Populus alba×glandulosa를 육성하기 위한 기초연구의 일환으로 2n-pollen의 대량획득 방법과 핵상별 anther culture의 최적방법을 규명하여 다음과 같은 결과를 얻었다. 1. Pectinase 1%, cellulase 2%, 그리고 D-mannitol 0.6M 등의 조성 enzyme액에서 pollen을 진탕처리한 결과 언 핵상의 pollen이나 80∼200분에서 cell wall이 용해되었다. 2. Pollen의 발아촉진을 위한 n-hexane처리의 회수와 핵상과는 관계가 없다. 3. Pollen의 X-ray에 대한 감수성은 핵상과 상관이 없고 고선량에서는 n과 2n-pollen 다같이 회분관 신장속도에 영향하였다. 4. Centrifuge force에 의한 n과 2n-pollen의 침강속도는 2n-pollen이 속하였다. 5. Anther culture를 위한 basic media와 plant hormone의 조성은 n-pollen에서는 W.S. basic media에 BAP 0.1ppm+NAA 0.5ppm이 가장 좋고 2n-pollen에서도 W.S. basic media에 BAP 0.1ppm NAA 0.5ppm이 가장 좋았다. The studies were carried out in order to induce vigorous growth through allotriploids of poplus alba<glandulosa. The results are as follows. 1. From n-, and 2n-pollen treated with enzyme solution containing 1% pectinase, 2% cellulase and 0.6M D-mannitol, the highest rate of pollen of cell wall dissolved were obtained in the 80 to 200 min. tretament. 2. n-, and 2n-pollen treated with n-hexane was no difference in the pollen germinatian. 3. n-, and 2n-pollen radiated with x-ray of 100-500kr was influence on the elongation speed of pollen tubes. 4. Sinking speed of pollen grains through centrifugal force was speedy in the tetraploid than in the diploid. 5. The production of callus through 2n-and 4n-anther culture varied with basic media and plant hormone, but good production was obtained from Wolter and Skoog medium containing 0.1ppm BAP and 0.5ppm NAA. 6. n-, and 2n-pollen plantleetsers were induced form populus alba×glandulosa anther cultured "in vitro" on synthetic medium.