흰쥐 Kupffer 세포에 대한 Propofol의 작용

박세훈,지대림,성언기,김희선,송인환,박희영,김준수,이덕희,박대팔 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: Propofol has an antioxidant capacity and can be used for ischemia-reperfusion injury of the liver. However, the effects of propofol on the Kupffer cells have not been establisked. Methods: Kupffer cells were isolated and cultured from male Sprague-Dawley rats. The effects of propofol on the Kupffer cells were evaluated by a phagocytosis assay, TNF-α gene expression, and superoxide anion release after administering propofol in different concentrations on the cultured Kuprrer cells. Results: The latex bead phagocytosis by the Kupffer cells was suppressed when the Kupffer cells were exposed to propofol irrespective of concentrations. Higher propofol concentrations decreased the loss of Kupffer cells after latex bead phagocytosis. Propofol induced TNF-α mRNA expression in the Kupffer cells, but the mRNA expression level after 50㎍/ml of propofol decreased. The pattern of TNF-α mRNA expression induced by propofol was different to that induced by LPS: TNF-α mRNA was expressed continuously in the propofol-treated cells until 16 hours after exposure to propofol, whereas the level of TNF-α mRNA expression induced by LPS was evident after 2 hours and was not found thereafter. TNF-α production after propofol treatment was not higher than that of the control. Formazan presipitation did not show any qualitative differences between cells untreated or treated with propofol concentrations of 0.5, 5.0, and 50 ㎍/ml. Conclusions: These results showed that propofol might inhibit Kupffer cells. This suggests that propofol and be used for patients with ischemia-reperfusion injury of the liver. (Korean J Anesthesiol 2002; 43: 475~484)

주제정의검사(Defining Issues Test)를 사용한 비행청소년의 도덕발달에 대한 연구

박병희,장경준,신지용 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.3

본 연구는 Rest가 고안하고 문용린이 번안한 주제정의검사(DIT)를 사용하여 청소년기의 도덕발달수준을 알아보고 비행청소년의 도덕발달수준과 비교하여 그 차이점에 대해 알아보고자 하였다. 먼저 신지용 등(1995)이 정상 고교생군에 대해 조사하였고 본 논문에서는 비행청소년군의 조사와 두 군의 비교 분석을 통해 청소년기의 도덕발달수준과 관련된 변인의 유의성을 검증하였다. 연구대상은 정상 고교생군 254명과 비행청소년군 285명이었다. 설문지 작성시 문항에 대한 이해 부족으로 생기는 오차를 줄이기 위하여 자세한 설명과 연습을 실시하였고 충분한 시간이 주어지도록 하였다. 수집된 자료는 컴퓨터로 채점되었으며 정상군의 자료와 함께 분석되었다. 연구의 결과는 다음과 같이 요약할 수 있다. 1) 정상 고교생군에 비해 비행청소년군은 도덕발달수준이 유의하게 낮게 나타났다(p<0.05). 2) 비행청소년군의 경우 종교에 따라 도덕발달수준의 유의한 차이를 보였으며 이는 천주교와 불교간의 수준차이로 검증되었다. 3) 정상고교생군과 비행청소년군의 PM점수 차이의 상관관계 분석 결과 아버지, 어머니의 교육수준 요인에서 주효과가 유의하게 나타났으며 주성장지는 상호작용 효과에 유의한 것으로 나타났다. Objectives : This study was directed to compare the moral developmental levels between normal and delinquent adolescent groups. And the authors also intended to find the demographic variables that influence the differences of moral levels between two groups. Methods : Defining Issue Test questionnaires were applied to 285 delinquent adolescents and the data were compared and analyzed with the normal control data of previous Shin's study(1995). Results : 1) The PM scores(scores of principled morality) of delinquent group(28.6±15.01) was significantly lower than that of normal control group, 41.8±15.10(p<0.05). 2) The PM scores were not significantly different according to the age in delinquent adolescents. 3) The PM scores were significantly different according to the religious varible, especially between Catholic and Buddhism in the delinquent adolescents. The Catholic group recorded the highest and Buddhism, the lowest. 4) Comparing the scores between normal and delinquent adolescents, there were significant differences according to the educational of mother and father, and the birth places. Conclusion : The moral developmental process of adolescents requires the proper education from parents, school, religion or other social environment. The corrective reexperience of normal developmental steps are needed especially to the delinquent adolescents.

小兒 驚風의 鍼灸治療에 對한 文獻的 考察

朴志修,金允姬,柳同烈 대전대학교 한의학연구소 2001 혜화의학회지 Vol.10 No.1

Infantile Convulsion, one of common emergency symptoms in pediatrics, arises from sudden derangement of the central nerve system, and can cause a sudden loss of consciousness and spasm. It falls into three categories; Acute Infantile cnvulsion, Chronic Infantile convulsion and Chronic Spleen convulsion. According to research, approximately 6~7% of all babies undergo spasm more than once. Since the treatment must be done immediately, acupuncture & moxibustion treatment can be one of the most important treatments in this particular case. Therefore, the focus of this study is on how acupuncuture & moxibustion can be utilized in the treatment of Infantile Convulsion, and the literary findings are as follows: 1. The meridian points used on acute infantile convulsion are Sugu(GV26), T´aech´ung(Liv3), Hapkok(LI4). 2. The meridians used on acute infantile convulsion are Governor Vessel(GV), Bladder Meridian(BL), Stomach Meridian(ST). 3. The meridian points used on accompanied symptoms with acute infantile convulsion are Haenggan(Liv2), Yangnungch´on(Liv3) on spasm, Paek´oe(GV14) on opisthotonus, Kokchi(LI11), Taech´u(GV14) on fever, Nogung(P8), Yongch´on(K1) on fainting spell, Chok-samri(S36) on body weakness. 4. The meridian points used on chronic infanitle convulsion are Shinguol(CV8), Ch´onchj´u(S25), T´aech´ung(Liv3), Kwanwon(CV4), Ch´ukt´aek(L5). 5. The meridians used on chronic infantile convulsion are Conception Vessel(CV), Governor Vessel(GV), Stomach Meridian(ST). 6. The meridian points used on accompanied symptoms with chronic infantile convulsion are Ch´onchj´u(S25), Kolli(CV11) on diarrhea, Taenung(P7), Shinmun(H7) on fainting spell, Kansu(B18), T´aech´ung(Liv3) on spasm. 7. The meridian points and meridians are Paek´oe(GV14), Sangsung(GV23), Sugu(GV26) of Governor Vessel(GV) and Chonjung(CV16), Shinguol(CV16) of Conception Vessel(CV) and Taedon(Liv1), Changmun(Liv13).

Celecoxib 유도체인 PCX-계열 화합물의 항염증 및 혈관신생 억제 작용

소지현,안은경,전혜진,임은주,박은희 숙명여자대학교 약학연구소 2005 약학논문집-숙명여자대학교 Vol.22 No.-

This study aimed to evaluate the pharmacological efficacies of PCX-series compounds, which were synthesized as prodrugs of celecoxib, a COX-2 selective inhibitor. Celecoxib, PCX-1, PCX-2, PCX-3, PCX-7, PCX-15 and PCX-16 provide potent anti-angiogenic activity in chorioallantoic membrane (CAM) assay. In addition, celecoxib, PCX-2, and PCX-16 showed anti-angiogenic activity in a dose-dependent manner. PCX-2 inhibited swelling in the carrageenan-induced rat paw edema test 24 hours after the administration. In the acetic acid-induced writhing test, PCX-7 and PCX-15 were verified to provide more potent analgesic effect than celecoxib 12 hours after the administration, and PCX-2 showed potent analgesic effect 24 hours after the administration. In the carrageenan-induced rat air pouch model, PCX-2 significantly decreased exudate volume, number of leukocyte and NO level. In the ulcerogenic activity test, PCX-2, PCX-7, PCX-15, and PCX-16 showed significantly less ulcer formation than indomethacin. Especially, PCX-7 showed the highest safety in the GI safety test. These findings provide novel pharmacological knowledge on pharmacological actions of celecoxib and its PCX-series compouds.

관동맥 우회로술 중 경식도 심초음파도로 관찰한 도부타민 투여후와 우회로술후의 좌심실 국소벽운동

김희열,김종진,윤호중,유기동,박지원,이길환,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.8

柴胡桂枝湯이 CCl₄로 誘發된 白鼠의 肝損傷에 미치는 影響

河智容,朴慶寔,李善熙 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.2

肝은 人體內의 血量을 調節하고 각종 大事, 分泌, 合成에 관여하는 중요한 기관이며, 病理的으로는 대사물질에 의한 중독에 민감하게 반응하여 간세포의 변성, 괴사, 지방축적, 간효소의 누출 등을 일으킨다. 간에 손상을 줄 수 잇는 요인 은 많으며, 최근에는 중독에 의한 손상이 증가하고 있다. 시호계지탕은 상한론에 ‘傷寒六七日 發熱徵惡寒 肢節煩疼 徵嘔 心下支結 外證未去者 柴胡桂枝湯主之에 사용된 처방으로 임상적으로 간손상에 사용되었으나, 실험적 연구는 실시되지 않앗다. 따라서 본 연구에서는 Sprague-Dawely 흰쥐에 사염화탄소로 중독성 간손상을 일으킨 후 시호계지탕을 투여하여 간손상에 미치는 영향을 알아보았다. Sprague-Dawely 흰쥐에 CCI₄와 Olive oil을 1:4로 혼합하여 4주간 격일로 경구투여하여 간손상을 유발하였다. 그 후 시호계지탕을 10㎎/㎏(B.W)로 하여 매일 경구투여 하였다. 실험 첫주부터 매주 각 군의 평균몸무게와 평균 사료섭취량을 측정하였고, 실험 마지막날 채혈한 뒤 순수혈청에서 AST, ALT, TP, Albumin, Gloulin, ALP, LDP를 검사 하였다. AST는 CCI₄處理群에 비하여 CCI₄處理後柴胡桂枝湯投與群 A와 CCI₄處理後柴胡桂枝湯投與群 B 모두 減少하였으며, CCI₄處理後柴胡桂枝湯投與群 B의 境遇는 有意性이 認定되었다. (P〈0.05). ALT는 CCI₄處理群에 비하여 CCI₄處理後柴胡桂枝湯投與群 A와 CCI₄處理後柴胡桂枝湯投與群 B 모두 유의성있는 감소를 보였다. (P〈0.05). CCI₄處理群과 比較하여 CCI₄處理後柴胡桂枝湯投與群 A와 CCI₄處理後柴胡桂枝湯投與群 B는 Tota protein의 경우 큰 차이가 없었으며, Albumin은 감소하였고, Globulin은 增加하였으나 有意性은 없었다. Albumin과 Globulin의 比率은 減少하였으나, 有意性은 없었다. ALP는 CCI₄處理群에 比하여 CCI₄處理後柴胡桂枝湯投與群 A와 CCI₄處理後柴胡桂枝湯投與群 B 모두 有意性있는 減少를 보였다.(P〈0.05). LDH는 특별한 傾向을 찾을 수 없었다. 肝의 重量을 測定한 結果 CCI₄處理後群에 比하여 CCI₄處理後柴胡桂枝湯投與群 A와 CCI₄處理後柴胡桂枝湯投與群 B 모두 有意性 있는 減少를 보였다. (P〈0,05). 體重과 飼科 攝取量의 變化는 CCI₄處理群에 比하여 CCI₄處理後柴胡桂枝湯投與群 A와 CCI₄處理後柴胡桂枝湯投與群 B이 增加하였으나 유의성은 없었다. 以上으로 보아 柴胡桂枝湯이 CCI₄로 誘發된 肝損傷의 境遇에 AST, ALT, ALP, 肝의 重量變化에서 有意性있는 減少(P〈0.05)를 보였으며, 體重과 飼科 攝取量의 增加로 보아 肝損傷 回復에 대한 治療效果가 있을 것으로 思料된다. This study was carried out to investigate the effects of Sihogyejitang extract on the recovery of liver in CCI₄-intoxicated rats. In this study, SD-Rats were divided into 4 experimental groups; Control(Placebo, 0.85% NaCl), Experi-mental (CCI₄-intoxicated+Placebo, 0.85% NaCl), Siho A(Treated with Sihogyejitang extract (10㎎/㎏) after CCI₄intoxication), Siho B(Treated with Sihogyejitang extract (50㎎/㎏) after CCI₄-intoxication), Biochemical assays for serum enzyme activities such as AST, ALT, TOtal protein, Albumin, Globulin, ALP, LDH, and Biological assay for Liver weight, Body weight, Food, intake were performed. The results obtained were summarized as follows: 1. Siho A group showed lower serum AST activity than CCI₄-intoxicated group significantly, and Siho B group showed lower than Control group(P〈0.05). 2. Both siho A group and siho B group showed lower serum ALT activity than CCI₄-intoxicated group significantly. 3. As to Total protein, Siho A group and Siho B group were classed with CCI₄-intoxicated group. As to Albumin, Siho A group and siho B group were decreased than CCI₄-intoxicated gruop, but not significant. As to Globulin, siho A group and B group were increased than CCI₄-intoxicated group, but not significant. As to Globulin, ratio siho A group and Siho B group were decreased than CCI₄-intoxicated group, but not significant. 4. As to ALP, Siho A group and Siho B group were decreased than CCI₄-intoxicated group significantly(P〈0,05). 5. As to serum LDH, There were no trend in all groups. 6. As to liver weight, Siho A group and Siho B group were decreased than CCI₄-intoxicated group significantly. 7. As to Body weight and Food intake, Siho A group and Siho B group were increased than CCI₄-intoxicated group, buy not significantly.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

機能性胃腸障碍 및 急性肝炎 患者에서 Clebopride에 依한 症狀의 好轉과 胃內容物排出 時間과의 關係

范熙承,金成烈,鞠敦杓,朴光淑,尹鍾晩,崔元,金文烈 최신의학사 1985 最新醫學 Vol.28 No.6

중등사회과 현직교사 연수프로그램의 실태와 개선방안

김성건,박희,김영우,이지경 서원대학교 2004 敎育發展 Vol.23 No.2

Home Health Care Classification(가정간호 분류체계)의 한글 명명화에 대한 타당성 연구

조인숙,황지인,박현경,최영희,이향련,박현애 성인간호학회 1999 성인간호학회지 Vol.11 No.2

The purpose of this study was to develop and test the validity of standardized Korean nomenclature of the Home Health Care Classification(HHCC), developed by Saba at the University of Georgetown. The four phases of the study were : (1) Two professors and 15 graduate students, who were taking a nursing intervention course, translated the HHCC into Korean. (2) 12 nurses with various clinical backgrounds reviewed each nomenclature taking into consideration of the definition and activities, and the Delphi method was used to determine the best appropriate nomenclature for each term; (3) 20 academic and clinical experts in nursing were given a questionnaire to rate the validity of each Korean nomenclature using a 5 point Likert scale ranging from very inappropriate to very appropriate : (4) Five members of the Korean Nurses Association Research Committee reviewed the survey results and determined the most appropriate Korean nomenclature for each term of the HHCC. Most of the nomenclatures had a score of more than 4.0, but several terms had a score lower than 4.0. They are Ambulation therapy(3.11), Anticipatory grieving(3.11), Sexual pattern alteration(3.17), Nursing care coordination(3.22), Physical therapist service(3.22), Regular diet(3.33), and Instrumental activities of daily living(3.44). These research results will be included in the nursing vocabulary dictionary to be published by the Korean Nurses Association.