四物湯 및 四君子湯이 Endotoxin으로 誘發된 血栓症에 미치는 影響

河智容,崔昇勳,安圭錫 慶熙大學校韓醫科大學韓醫學硏究所 1988 慶熙韓醫大論文集 Vol.11 No.-

淸上補下丸이 黑色腫의 肺轉移 抑制 및 免疫調節作用에 미치는 影響

河智容,劉炳吉 대한한방종양학회 1998 대한한방종양학회지 Vol.4 No.1

1. 연구 배경 최근 놀라운 과학기술의 발전에 힘입어 난치병이라고 생각되어 오던 질환들이 하나씩 정복되어 가고 있으나 평균수명의 연장, 생활수준 향상으로 인한 고지방식, 스트레스 및 과학기술발전의 부작용 등을 이유로 새로운 질환들이 나타나고 있는 실정이다. 종양도 이런 대표적인 질환중 하나로 최근 경제적, 시간적으로 많은 투자를 해왔으나 아직까지 그 발생원인이나 성장기전 등이 자세히 밝혀지지 않았다. 이에 본 연구는 哮吼를 치료하는 淸上補下丸을 이용하여 흑생정의 폐전이 억제능과 이에 의한 면역증강에 관하여 연구하고자 하였다. 2. 연구결과 B-16세포를 이용한 C57BL/6계 마우스에 있어서의 폐전이 억제능을 측정한 결과 淸上補下丸 투여군이 대조군에 비해 검액처리 7일후에는 52.70%, 14일후에는 19.43%의 유의성있는 억제율을 보였으며, 淸上補下丸의 B-16 세포에 대한 직접적인 항암작용을 알아 보기 위한 시험관내 세포독성 실험에서는 淸上補下丸의 용량이 5ug/well, 2.5ug/well, 1.25ug/well, 0.625ug/well인 경우 유의성있는 효과를 얻을수 있었으며 생존율에 의한 IC_50은 2.17ug/well이었다. NK-activity를 측정한 결과 淸上補下丸 투여군이 작동세포와 표적세포의 비율이 100:1, 50:1의 경우에 있어서 72.63±5.17%, 26.94±2.43%를 보여 대조군에 비하여 각각 31.12%, 43.68% 유의성있는 증가를 보였다. IL.2의 생산능을 측정한 결과 淸上補下丸 투여군은 l392.00±22.3lpg/ml을 보여 대조군에 비해 4.04%의 유의성있는 증가를 보였다. In order to investigate the antitumor effect by Chungsangbohahwan after B-16 cells were transplanted in C57BL/6 mice, and the immune responses in mice induced by methotrexate, the extract of Chungsangbohahwan sas orally administered to the mice for 21 days. Experimental studies were performed for measurce of metatasis, cell cytotoxicity in vitro, natura killer cell activity, productivity of interleukin-2. The results were summarized as follows: 1. Inhibition of metastasis in Chungsangbohahwan-treated group was higher than control group woith significance on 7th day and 14th day. 2. On the MTT assay, cell viability was signigicantly inhibited by 5ug/well, 2.5ug/well, 1.25ug/well, and 0.625ug/well of Chunganbohahwan concentration inbited cell viiability sifnificantly(P<0.05). IC_50 for cell viability was 2.17ug/well. 3. Matural killer cell activity in Chungsangbohahwan-treated group was significantly incresed on 100:1, 30:1 E/T(effect ce;;/target ce;;) rato(P<0.05). 4. Produciton of interleukin-2 in Chungsangbohahwan-treated group was significantly increased(P<0.05).

靑皮가 CCL₄로 誘發된 Rat의 肝損傷에 미치는 影響

李桓容,河智容 尙志大學校 1996 論文集 Vol.17 No.-

This study was proposed to investigate the effect of Chungpi extract on the recovery from liver injury in CCL₄-intoxicated rats. For this study, Sprague-Dawley rats were divided into 3 experimental group : control group, DDL₄-intoxicated group, group treated with Chungpi extract after CCL₄-intoxcation. Biochemical assays serum emzyme activity of G.O.T., G.P.T., d-dilirubin were performed. The resurts obtained are summarized as follows: 1. In macroscopic observation, Chungpi treated group was clear than CCL₄-intoxicated group. 2. Chungpi treated group showed significantly lower serum G.O.T. activity than CCl₄-intoxicated group. 3. Chungpi treated goup showed lower serum G.P.T. activity than CCL₄-intoxicated group, but no significant. 4. Chungpi treated group showed significantly lower serum d-Bilirubint activity tna CCL₄-intoxicated group. From above results, it is suggested that Chungpi has a valid and curative effects on the recovery from liver injury.

다양한 약침제제(藥鍼製劑)가 Rat의 Adjuvant 관절염(關節炎)에 미치는 영향(影響)

하지용,이선구,유병길,Ha, Jee-Yong,Lee, Seon-Goo,Yu, Byeong-Gil 대한약침학회 2000 Journal of pharmacopuncture Vol.3 No.2

To investigate effects of vanous herbal-acupuncture, which were Daebangpungtang, Oyaksungisan, Dokhwalgisaengtang, and Binsosan on Adjuvant Arthritis in rats, the edema rate, the number of WBC, the quantity of TNF-${\alpa}$, COX -2, and IL-6, and histological test of the muscular tissue were measured in the arthritis part. After elicilating arthritis of Sprague Dawely(SD) rats by injection of Freund's complets adjuvant for 2 weeks, saline was injected for the control group, Daebangpungtang acua-acupuncture was injected for the Daebangpungtang group, Oyaksungisan acua-acupuncture was injected for the Oyaksungisan group, Dokhwalgisaengtang acua-acupuncture was injected for the Dokhwalgisaengtang group, and Binsosan acua-acupuncture was injected for the Binsosan group during 30days. Selected point was ST35 in all groups. 1. The number of WBC was $8.24{\pm}0.51$($10^3$/ml) in the normal group and $27.35{\pm}3.51$($10^3$/ml) in the control group. the Daebangpungtang group and the Oyaksungisan group decreased each $20.42{\pm}2.75$($10^3$/ml) and $19.78{\pm}4.99$($10^3$/ml) in the Exp.ll group. This fact showed that the group Exp.ll was more effective than the control group effectively. (P<0.05) 2. The volume of the paw were checked. The volume of paw was $0.62{\pm}0.11mm in the control group and $0.45{\pm}0.08$mm in the Daebangpungtang group and $0.47{\pm}0.07$mm in the Oyaksungisan group, the swelling of the paw was restricted significantly in the Daebangpungtang and Oyaksungisan group(P<0.05) 3. The bands of the TNF-${\alpa}$, COX -2 in the muscular tissue of the control group wcre certain and thick than other groups(except normal group), and the band of the IL-6 in the muscular tissue of all group(except normal group) were similar forms. 4. In histological finding, because of severe inflammatory reaction, remarkably irregular tissue and large amount of inflammatory cells were found in thc control and Dokhwalgisaengtang group. But the Oyaksungisan and Daebangpul1gtang group showed small amount of inflammatory cells, the refrained inflammatory state and even recovering state. From these results, it is showed Oyaksugisan and Daebangpungtang acua-acupuncturc refrain inflammatory reaction and muscular tissue necrosis in SD rats paw were induced by Freund's complete adjuvant

포공영(蒲公英) 약침(藥鍼)이 Rat의 Adjuvant 관절염(關節炎)에 미치는 영향(影響)

하지용,Ha, Ji-Yong 대한약침학회 1999 Journal of pharmacopuncture Vol.2 No.1

To investigate effects of Taraxaci herbal-acupuncture on Adjuvant Arthritis in rats, the edema rate, the number of WBC, the quantity of total protein, albumin and globulin in the blood serum and histological test of the muscular tissue were measured in the arthritis part. 1. After elicitating arthritis of Sprague Dawely(SD) rats by injection of Freund's complets adjuvant for 2 weeks, saline was injected for the Exp. I group and Taraxaci herbal-acupuncture was injected for the Exp. II group during 30days. Selected point was $ST_{35}$ in both the groups. And then the volume of the paw were checked. The volume of paw was $0.84{\pm}0.14mm$ in the Exp. I group and $0.38{\pm}0.17mm$ in the Exp. II group, the swelling of the paw was restricted significantly in the Exp. II group(p<0.05) 2. The number of WBC was $10.34{\pm}0.14(10^3/ml)$ in the normal group and $37.47{\pm}5.46(10^3/ml)$ in the Exp. I group. It was $20.39{\pm}4.23(10^3/ml)$ in the Exp. II group. This fact showed that the group Exp. II was more effective than the Exp. I group in the treatment of arthritis(p<0.05) 3. The content of the total protein in the blood serum was $6.14{\pm}0.43g/dl$ in the normal group, $7.95{\pm}0.94g/dl$ in the Exp. I group, and $6.38{\pm}1.75g/dl$ in the Exp. II group. This fact showed that the group Exp. II was more effective than the Exp. I group in the treatment of arthritis(p<0.05) 4. The contents of albumin in the blood serum was $2.94{\pm}0.13g/dl$ in the normal group, $2.01{\pm}0.48g/dl$ in the Exp. I group. and $2.71{\pm}0.34g/dl$ in the Exp. II group. This fact showed that the group Exp. II was more effective than the Exp. I group in the treatment of arthritis(p<0.05) 5. The contents of globulin in the blood serum was $3.19{\pm}0.48g/dl$ in the normal group, $4.70{\pm}1.26g/dl$ in the Exp. I group. and $3.67{\pm}0.56g/dl$ in the Exp. II group. There was no significance in the serum globulin between Exp. II group and Exp. I group from the stastical analysis 6. In histological finding, because of severe inflammatory reaction, remarkably irregular tissue and large amount of inflammatory cells were found in the Exp. I group. But the Exp. II group showed small amount of inflammatory cells, the refrained inflammatory state and even recovering state. From these results, it is showed Taraxaci herbal-acupuncture refrain inflammatory reaction and muscular tissue necrosis in SD rats paw were induced by Freund's complete adjuvant

The Efficacy and Safety of a Human Perirenal Adipose Tissue-Derived Stromal Vascular Fraction in an Interstitial Cystitis Rat Model

하지용,Lee Eun Hye,Chun So Young,Lee Jun Nyung,Ha Yun-Sok,Chung Jae-Wook,윤보현,Jeon Minji,Kim Hyun Tae,Kwon Tae Gyun,Yoo Eun Sang,Kim Bum Soo 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.2

BACKGROUND: Interstitial cystitis (IC) is a chronic and intractable disease that can severely deteriorate patients’ quality of life. Recently, stem cell therapy has been introduced as a promising alternative treatment for IC in animal models. We aimed to verify the efficacy and safety of the human perirenal adipose tissue-derived stromal vascular fraction (SVF) in an IC rat model. METHODS: From eight-week-old female rats, an IC rat model was established by subcutaneous injection of 200 lg of uroplakin3A. The SVF was injected into the bladder submucosal layer of IC rats, and pain scale analysis, awakening cytometry, and histological and gene analyses of the bladder were performed. For the in vivo safety analysis, genomic DNA purification and histological analysis were also performed to check tumorigenicity and thrombus formation. RESULTS: The mean pain scores in the SVF 20 ll group were significantly lower on days 7 and 14 than those in the control group, and bladder intercontraction intervals were significantly improved in the SVF groups in a dose-dependent manner. Regeneration of the bladder epithelium, basement membrane, and lamina propria was observed in the SVF group. In the SVF groups, however, bladder fibrosis and the expression of inflammatory markers were not significantly improved compared to those in the control group. CONCLUSION: This study demonstrated that a perirenal adipose tissue-derived SVF is a promising alternative for the management of IC in terms of improving bladder pain and overactivity.

Guinea pig의 膽囊色素石 病態模型과 이에 미치는 大柴胡湯 및 大柴胡湯加金錢草의 效能에 關한 硏究

崔昇勳,安圭錫,文濬典,河智容 대한동의병리학회 1992 동의생리병리학회지 Vol.7 No.-

In order to investigate the effects of Daeshihotang, Daeshihotang and Desmodium stractyfolium guinea pig which were fed with lithogenic diet were administered solid extracts of Daeshihotang, Daeshihotang and Desmodium stractyfolium and then observed about gallstone incidence, β-glucuronidase in bile, in serum, bilirubin in bile, in serum, bile total cholesterol The results were obtained as follows 1. Pigment stone were formed by lithogenic diet in guinea pig and the incidence rates of gallstone were reduced in Daeshihotang, Daeshihotang and Desmodium stractyfolium group. 2. The level of β-glucuronidase in bile was remarkably increased in control group and in Daeshihotang and Desmodium stractyfolium the level of β-glucuronidase was reduced as compared with that of control group. 3. The level of β-glucuronidase in serum also remarkably increased and it reduced in control group but had no statistical significance. 4. The level of total bilirubin in bile was remarkably increased in control group, and had attendancy of reduce in Daeshihotang, Daeshihotang and Desmodium stractyfolium. The level of free bilirubin also significantly increased in control group and had attendancy to reduce in Daeshihotang, Daeshihotang and Desmodium stractyfolium group. The rate of free bilirubin to the total bilirubin was most increased in control group and reduced in Daeshihotang, Daeshihotang and Desmodium stractyfolium group. 5. The level total bilirubin in serum was remarkably increased in control group but the rate of free bilirubin to the total bilirubin in bile had little changes and free bilirubin in serum was increased as compared with that of bile. 6. The level of total cholesterol was increased in control group and Daeshihotang, Daeshihotang and Desmodium stractyfolium group showed similiar result. According to the above results, it is considered that Daeshihotang, Daeshihotang and Desmodium stractyfolium have therapeutic effects on cholecystolithiasis induced by lithogenic diet in guinea pig. Clinically we can make use of Daeshihotang, Daeshihotang and Desmodium stractyfolium for therapeutic and preventive effects on cholecystolithiasis but the mechanism concerned for the effects is to be investigated detaily in future.

蜂毒(Apitoxin)이 흰 쥐의 Adjuvant 關節炎에 미치는 影響

朴亨奎,安圭錫,河智容 대한동의병리학회 1993 동의생리병리학회지 Vol.8 No.-

연구배경 : 봉독요법은 아직 보편화되지 못했으나 실제 임상에서 만성 관절염이나 염증질환에 많이 이용하고 있으며, 수천년전부터 민간요법으로 사용되어 왔다. 이에 봉독의 치료효과를 과학적으로 검증하고 치료기전을 밝혀 봉독요법의 활용에 도움이 되고자 한다. 방법 : 실험용 흰쥐에 Freun's complete adjuvant 액을 피하주사하여 관절염을 유발시키면서, apitoxin을 피하주사하여 부종률, 진통효과 및 소염작용을 측정한다. 결과 : 치료군에서 부종률이 억제되었고 WBC 및 혈청 cholinestrase의 활성도가 감소되었으며, 동통역치는 증가하였다. 결론 : 봉독요법은 소염, 진통에 뛰어난 효능을 가지고 있어 비증, 역절풍 및 통풍 등에 응용할 수 있을 것으로 사려된다. In order to study the effect of Apitoxin therapy on the Anti-inflammatary and Analgesic Action, Apitoxin therapy was administered on the Arthritis induced by Freund's complete adjuvant in rats. The obtainded results were summarized as follows. 1. Anti-inflammatory effect of Apitoxin therapy on the Anti-inflammatory action was showed statistical significance. The Sample A was more excellent on the decrease of edema and the number of WBC. 2. Analgesic Effect was showed with significance in sample A and B. Sample A was more excellemt in pain threshold, and B was more excellent in decrease of cholinestrase. According to the above result, it can be concluded Bee Vemom therapy showed the Anti-inflamentory and Analgenic Effect, and is suggest that the more interest, study in the secuity for the clinical use were neded.

頸項痛에 대한 東醫學的 病理 및 臨床資料

河智容 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.1

역대문헌에 있어 경항통에 관하여 특별히 기술한 곳은 그리 많지 않는데 이는 그 당시 경항통의 질환이 없었다기보다는 여타 질환에 비해 그 빈도 및 중요성이 떨어진다고 보았을 것으로 보여진다. 그러나 시대의 변천에 따라 의학계에 있어서도 많은 질환의 치료법이 개발되었으며 스트레스, 불량한 자세의 장시간 작업 등 여러 정신적 환경적 요인에 의해 경항통, 특히 추간판탈출증의 질환이 대두되어 이에 역대 의가들의 병리학적 사고와 치료법을 검증하고자 한다. 경항부위에 있어 해부학적 위치와 구조에 관하여 기술하고 그에 따른 구조적 기능손상에 의하여 통증을 유발하는 질환을 열거하여 현 서양의학에서 말하는 경항통의 질환을 기술하였고 그다음 한의학에 있어서 역대 의가들의 경항통에 관한 병리학적 사고와 이의 변증방법 및 치료법을 열거하였다. 한의학에서 경항통의 원인을 대개 풍한(風寒), 풍습(風濕)으로 보아 태양병(太陽病)의 범주에 넣어 발산지제(發散之劑)로 치료하였지만 그 외에 상실하허(上實下虛) 및 간신허손(肝腎虛損)으로 보아 보음지제(補陰之劑)를 사용하는 경우도 있었으며 치료에 있어서도 단순히 침구와 처방외에 훈세법(熏洗法), 견인요법(牽引療法), 수침요법(水鍼療法), 추나요법(推拿療法) 등을 병행하기도 하였다. Neck pain was accoused singly or compoundly by surgical trauma of neck, affection due to external wind-cold, wind-damp, weakness. This disease reported less than others in past oriental area, but it has become important for the necessity of complete treatment of neck pain, especially for the which come from the psychiatry and environment of working. What doctors through all ages have researched were summarized as follow: 1. Cause of disease was generally affection due to external wind-cold and wind-damp. Minor was upper excess and lower deficiency, deficiency in both the liver and kidney. 2. Prescription was choiced either dispersing or invigorating yin of drugs by cause. 3. Treatments were prescription, acupuncture and moxibustion, steaming and washing method, therapy of extension, fluid acupuncture therapy, massage manipulation. 4. Preventing methods was suitable pose in working, the careful adjustment of daily life, prevention of external affections, avoidance of sexual indulgence, proper movement.

鳥藥順氣散 藥針이 Rat의 Adjuvant 關節炎에 미치는 影響

河智容,李膳求,劉炳吉 대한동의병리학회 2000 동의생리병리학회지 Vol.14 No.2

최근 식생활의 변화, 운동부족, 평균연령의 증가 등의 여러 요인에 의하려 류마토이드 關節炎, 통풍성 關節炎, 골 關節炎 등의 관절질환이 늘어나고 있는 실정이다. 미국의 경우는 전국민의 2-3%가 關節炎에 이환 된 것으로 보고되고 있으며, 關節炎치료에 대한 硏究가 활발히 이루어지고 있다. 독일, 스위스, 일본 등 선진 여러 나라에서도 그 실태는 미국과 유사하며, 여러 연구기관에서 다방면의 硏究를 수행하고 있다. 烏藥順氣散은 ‘治一切風疾, 先服此, 疎通氣道, 進以風藥, 이치탄탄 , 及歷節風’治療효능이 있는 處方으로, 臨床的으로 많이 사용되고 있느나, 關節炎 모형에 대한 實驗的 硏究는 아직 시행되어 있지 않았다. 본 硏究는 實驗的으로 유발된 關節炎에 烏藥順氣散 약침을 犢鼻穴에 처치하여 나타난 결과이다. 1. 식염수로 처리한 대조군의 후족 용적은 0.62±0.11㎜를 나타냈고 烏藥順氣散처리군은 0.47±0.07㎜로 부종의 용적이 유의성 있게 억제되었다(P<0.05). 2. 정상군(Normal group)의 경우 백혈구 총수는 8.24±0.51(103/㎖), 식염수로 처리한 대조군의 백혈구 총수는 27.35±3.51(103㎖) 이었다. 烏藥順氣散처리군은 19.78±4.99(103㎖)로 대조군 보다 낮은 수치를 보여 關節炎 치료에 효과를 보였다(P<0.05). 3. TNF-α의 경우는 정상군에서 극히 소량의 m-RNA가 검출되어 염증 유발군과 비교해 볼 때 상당히 차이를 나타냈다. 염증유발군간에는 대조군이 약침처리군 보다 band의 size가 크고 선명하여 TNF-α의 량이 많음을 나타내어 염증 상태가 보다 더 심각함을 보여 주었다. 4. IL-6는 정상군에서 극히 소량으로 검출 되었으나 염증유발군에서는 상당한 크기로 선명하게 검출되어 염증상태가 심각함을 보여 주었다. 5. 조직 검사 결과 대조군에서는 염증반응이 심하게 나타나 조직의 형태가 매우 불규칙하고 다수의 염증 세포 등이 보였고 烏藥順氣散 약침처리군은 조직이 다소 회복된 상태의 증후가 보이고 염증세포의 수가 줄고 염증상태가 억제됨을 알수 있었다. 이상의 결과를 종합하여 보면 烏藥順氣散의 犢鼻穴 藥針 처리가 Freun's complete adjuvant 關節炎 유발 rat에 작용하여 염증을 억제한 것을 알 수 있었다. 그러나 이러한 효과에 대한 좀더 지속적인 연구가 필요하다고 생각한다. Effects of Oyaksunkisan acua-acupuncture on Adjuvant Arthritis in rats. The study was carried out to investigate the effects of aqua-acupuncture with Oyaksunkisan on the counts of Edema, WBC and m-RNA level of TNF-α, IL-6and COX-2 in rat with Freund's adjuvant arthritis. The size of edema showed a tendency to increased in the group of Freund's adjuvant arthristis compared with those of normal condition. In the groups of Freund's adjuvant arthristis, the size of edema showed hight in the control group compared with those of aqua-acupuncture group. The counts of WBC showed a tendency to increase in rat with Freund's adjuvant arthristis compared with those of normal condition, In the groups of Freund's adjuvant arthristis, the counts of WBC showed hight in control group compared with aqua-acupuncture group. In the RT-PCR, the mRNA of TNF-α, IL-6 and COX-2 were detected very low level in the normal condition compared with those of Freund's adjuvant arthristis. However in the groups of Freund's arthristis, the values showed no difference. In histological finding, because of severe inflammatory reaction, remarkably irregular tissue and large amount of inflammatory cells were found in the control group. But the aqua-acupuncture with Oyaksunkisan group showed small amount of inflammatory cells, the refrained inflammatory state and even recovering state. From these results, it is showed Oyaksunkisan acua-acupuncture refrain inflammatory reaction and muscular tissue necrosis in DS rats paw were induced by Freund's complete adjuvant. Effects of Oyaksunkisan acua-acupuncture on Adjuvant Arthritis in rats. The study was carried out to investigate the effects of aqua-acupuncture with Oyaksunkisan on the counts of Edema, WBC and m-RNA level of TNF-α, IL-6and COX-2 in rat with Freund's adjuvant arthritis. The size of edema showed a tendency to increased in the group of Freund's adjuvant arthristis compared with those of normal condition. In the groups of Freund's adjuvant arthristis, the size of edema showed hight in the control group compared with those of aqua-acupuncture group. The counts of WBC showed a tendency to increase in rat with Freund's adjuvant arthristis compared with those of normal condition, In the groups of Freund's adjuvant arthristis, the counts of WBC showed hight in control group compared with aqua-acupuncture group. In the RT-PCR, the mRNA of TNF-α, IL-6 and COX-2 were detected very low level in the normal condition compared with those of Freund's adjuvant arthristis. However in the groups of Freund's arthristis, the values showed no difference. In histological finding, because of severe inflammatory reaction, remarkably irregular tissue and large amount of inflammatory cells were found in the control group. But the aqua-acupuncture with Oyaksunkisan group showed small amount of inflammatory cells, the refrained inflammatory state and even recovering state. From these results, it is showed Oyaksunkisan acua-acupuncture refrain inflammatory reaction and muscular tissue necrosis in DS rats paw were induced by Freund's complete adjuvant.