Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

놀이중심 사회적 능력 증진 프로그램의 효과 연구: 발달지체 유아를 대상으로

정계숙,노진형,박명화 부산대학교 영유아보육연구소 2001 영유아보육연구 Vol.7 No.-

This study purposed to examine the effects of the social competence program for improving the social competence of developmentally delayed young children referred to the Child Intervention and Counseling Center, Pusan National University. The subjects were four 3-4 year-old boys. Three of them were diagnosed as developmental delays or child with autistic behavioral characteristics by child psychiatric doctors. One boy was not diagnosed by the doctor, but was identified as developmental delays which may be caused from reactive attachment disorder in the process of the diagnostic assessment by the researchers. The social competence program was composed of 16 sessions which had some structured and unstructured play activities selected from several social competence related intervention programs for young children with social-emotional difficulties. The length of the program was one and half hours and the ratio of child vs adult was 2:1. At every after-session meetings for on-going monitoring to the children's behaviors, the intervention team members consisted of the researcher, intervention teachers, observers and parent meeting leaders exchanged the informations about the children each other and discussed about the better intervention practices of the next session. To analysis the effects of the social competence program, observation data obtained by 2 trained observers in the observation room at every sessions and the informations about child's behaviors and changes in home and other life settings gathered from mothers at every parent meetings were analyzed qualitatively in terms of each child's objective behaviors. In spite of some individual differences, the young children with developmental delays showed more positive and active social behaviors with their intervention teachers in the center and mothers and siblings in home generally than before the program. It suggested some implications for improving the practices of the social competence program for developmentally delayed young children with socal-emotional difficulties.

창조적 지식 기반 사회를 대비한 국어과 교육의 목표와 내용

정혜승,노명완,옥현진 이화여자대학교 교육과학연구소 2000 교육과학연구 Vol.31 No.1

본고는 창조적 지식 기반사회에서 가장 중요한 국어과 교육의 목표는 언어 사용 기능의 신장이며, 이를 실현시키기 위한 교육 내용에 대해 논의하였다. 일부 국어교육학계에서 기능은 하찮고 저열한 것으로 인식되기도 하였으나, 이는 지식의 개념을 명제적 지식에 국한하여 생각하고 기능을 지식과 별개의 것으로 잘못 인식한 데서 기인한 것이다. 본고는 명제적 지식과 방법적 지식의 관계를 탐구하여 기능 학습에 관한 이론을 두 가지로 정리하였다. 하나는 명제적 지식을 우선하여 이를 연습하고 내재화하는 가운데 기능 학습이 이루어진다고 보는 것이며, 다른 하나는 명제적 지식을 전제하지 않고 다양한 경험과 활동을 통해 기능 학습이 이루어진다는 것이다. 전자는 학습 활동이 용이하고 체계적이라는 점에서, 후자는 앎의 과정을 보다 중시한다는 점에서 장점을 갖고 있으나, 한 편으로 전자는 명제적 지식의 성격이 기능 학습에 유효한 것이어야 한다는 검과, 후자는 실제 수업이 이루어지기 위해서 치밀한 계획과 준비가 필요하다는 점에서 문제점도 안고있었다. 3차 국어과 교육과정은 후자의 방법을, 6차와 7차 국어과 교육과정은 전자와 같은 인식을 보이고 있으나, 논의가 개념 수준에서 이루어져 언어 사용 기능 교육이 제대로 이루어지기 어렵다는 한계를 갖는다. 따라서 국어과 교육의 논의는 개념 수준에서 벗어나 실시간과 실공간 속에서 학생들이 무엇을 어떻게 배울 것인지를 구체적으로 제시하는, 곧 수업화ㆍ자료화하는 실질적이고도 기술적인(technical) 논의로 바뀌어야 한다고 본다. Aim and Content of Korean Language Education for a Society Based on Creative Knowledge This thesis argues the improvement of language use skill that is the most important aim of Korean language education in a society based on creative knowledge and the education contents to achieve it. Some Korean language educators regard skill as worthlessness and vulgarity. However it results from the misunderstanding that the concept of knowledge is limited to knowing that and that skill is entirely different from knowledge. This paper summarizes the theory on skill study in two ways by researching the relations between knowing that and Know-how. One is that skill study is done in the process of exercising and internalizing knowing that preferentially and the other is that it's done through a lot of experiences and actives without any premise of knowing that. These have respective merits : former has the easiness and systematic of skill study and the latter thinks highly of the processing of knowledge. On the contrary, there are problems : in case of the former, the content of knowing that should be effective to skill study and the latter needs accurate plan and preparation for actual study. The 3rd education course in Korean language with latter method and the 6th and 7th without former on place a limit that the argument only in concept level makes the actual language use skill difficult. Therefore the argument of Korean language education should be changed to actual and technical one to present in detail what and how students learn in real time and real space.

림프절 생검으로 진단된 복강내 간질섬유형성 소원형 세포 종양 1예 보고

주명진,이광민,이혜경,정동규 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.5

과학수업모형의 비교 분석 및 내용과 활동 유형에 따른 적정 과학수업모형의 고안

정완호,권재술,최병순,정진우,김효남,허명 한국교원대학교 과학교육연구소 1996 청람과학교육연구논총 Vol.6 No.1

The purpose of this study is to develop appropriate science teaching models which can be applied effectively to relevant situations. Five science teaching models; cognitive conflict teaching model, generative teaching model, learning cycle teaching model, hypothesis verification teaching model and discovery teaching model, were identified from the existing models. The teaching models were modified and in primary and secondary students using a nonequivalent pretest-posttest control group design. Major findings of this study were as follows: 1. For teaching science concepts, three teaching models were found more effective; cognitive conflict teaching model, generative teaching model and discovery teaching model. 2. For teaching inquiry skills, two teaching models were found more effective; learning cycle teaching model and hypothesis verification teaching model. 3. For teaching scientific attitudes, two teaching models were found more effective; learning cycle teaching model and discovery teaching model. Each teaching model requires specific learning environment. It is strongly suggested that teachers should select a suitable teaching model carefully after evaluating the learning environment including teacher and student variables, learning objectives and curricular materials.

기니픽 망막 신경원에서 Dopamine과 GABA의 공존

천명훈,오수자,이문용,정진웅 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.1

설치류 망막의 도파민성 신경원의 분포상태, 미세구조 및 연접기구에 대한 연구 - Ⅱ.미세구조 및 연접기구

천명훈,이문용,한승호,오수자,정진웅 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.2

전자거래에서의 표시·광고의 법적 문제

鄭震明 법무부 2004 선진상사법률연구 Vol.- No.21

It is suggested that new legal regulation is necessary in electronic commerce. It has two main reasons. First, consumers depend on the information which a business person offers labelling and advertisement when they buy goods or services. Second reason is that fraud labelling and advertisement might be various in electronic commerce much more than facial contract or written contract because electronic commerce requires prompt decision for purchasing and it needs no facial contract. Therefore, it is required that the way of choice and decision buying goods or services should be broadened for consumer and one of liberty for business activities maximized for a business person. In this paper, integration of labelling and advertisement, injustice of their contents and randomness of unsolicited commercial e-mail were examined for the legal matter of labelling and advertisement. Among these, less spending time and expense of the labelling and advertisement make it difficult that legal regulation is put on a business person. So, it is required that consumers should assure the authenticity of labelling and advertisement which is related to electronic commerce. In this paper, a reform measure is explored being based on the explanation of regulation. Consumers have difficulty of using the labelling and advertisement by their technical development in electronic commerce. Therefore, it is required for the way that consumers understand the information of labelling and advertisement and a business person and consumers be responsible for each other's contract. Furthermore, the labelling and advertisement has still been commercialized by the business person even when consumers do not want, which causes that consumers can not purchase goods or services by their own free decision. This makes it necessary that legal regulation be intervened to be the trading circumstances like the free competitive market for consumers. In this paper, for this, the legal system is explored, which needs to have improvement for labelling and advertisement for the fairness of trade and then needs regulate the business person's responsibility who is participated in the trade. Finally, it is groped that consumers can get the right information on goods or services and trading circumstance can be made for the free trade through not fraud information and then the method of the fair competition can be obtained to keep it for between business people. 전자거래에서는 기존의 거래와 달리 표시·광고와 관련하여 새로운 규제의 필요성이 제기되고 있다. 왜냐하면 전자거래에서 소비자는 그가 물품 또는 서비스를 구입하는데 있어서 판단자료가 되는 주요정보를 대부분 사업자가 제공하는 표시·광고에 의존하기 때문이다. 또한 전자거래에서는 거래당사자의 비대면성과 정보에 대한 즉시적인 판단이 요구되므로 대면거래나 서면거래에 비하여 각종의 사기적인 표시·광고가 행하여질 가능성이 크기 때문이다. 그러므로 소비자에게는 물품이나 서비스의 구매결정에 대한 선택의 폭을 확대하고, 사업자에게는 영업활동의 자유를 최대한 보장할 수 있는 방안이 요청된다. 첫째, 전자거래에서의 표시·광고와 관련하여서는 표시·광고의 일체성, 광고내용의 부당성, 원하지 않는 광고성 전자우편의 무차별적 전송 등이 문제되고 있다. 이러한 문제들 가운데 전자거래에 있어서는 특히 표시·광고에 드는 시간과 비용이 절감됨에 따라 시장에 대한 직접적인 규제가 효율적으로 이루어질 수 없다. 이를 위해서는 전자거래에서의 표시·광고와 관련하여 소비자가 표시·광고의 진실성을 확보할 수 있는 방안이 요청된다. 따라서 기존의 법규정에 대한 해석을 토대로 하여 새로운 전자거래 환경에 적합한 표시·광고의 개선방향을 모색하여 보았다. 둘째, 전자거래에 있어서 표시·광고는 표시·광고 기법의 발달에 따라 소비자들이 이들을 활용하는데 어려움이 있다. 그러므로 소비자가 보다 쉽게 표시·광고를 이해하고 당사자 사이의 책임을 분명히 할 수 있는 방안이 요청된다. 나아가 전자거래에서의 표시·광고는 소비자의 요청이 없음에도 불구하고 사업자가 개별적으로 광고행위를 하고 이를 통하여 소비자가 자신의 자유로운 의사결정에 따라 상품을 구매할 수 없는 상황을 야기한다. 그러므로 전자거래에서의 표시·광고와 관련하여서는 소비자에게 자유경쟁 시장과 같은 거래환경을 조성하기 위한 법제적 노력이 요구된다. 이를 위해서는 거래의 공정성을 확보할 수 있도록 표시·광고메커니즘을 개편하며, 나아가 거래에 참여하는 사업자의 책임을 분명하게 규정할 수 있는 법제도의 마련에 대하여도 고찰하여 보였다. 결론적으로 이 글에서는 전자거래에서의 표시·광고와 관련하여 소비자들이 물품이나 서비스에 대한 정확한 정보를 취득하여 자유로운 구매행위가 이루어 질 수 있도록 거래환경을 조성하고, 이와 함께 사업자간 공정경쟁이 유지될 수 있도록 공정경쟁의 확보방안이 필요함을 강조하였다.

안구적출에 따른 위둔덕의 Calbindin D-28k과 c-fos의 변화에 관한 연구

김명,김기훈,김주영,하현철,안명수,김장민,조향훈,정명섭,장인엽 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Changes of Calbindin D-28k- and c-fos-immunoreactivities in the superior collicuclus after Eye Enucleation. Objective and methods: Calcium-binding proteins (CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k were examined immunohistochemically in the superficial layer of the rat superior colliculus. Also early gene familly c-fos was examined to evaluate the neuronal characteristics in the superior colliculus after monocular enucleation. Results: On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive (IR) cells was reduced (77.4% compared to control), but not fibers. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side within 24h after eye enucleation. Conclusion: These results suggest that the changes of retinotectal projection may alter the expressional pattern of calbindin D-28k and c-fos expression.