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실험적 각막 상피세포 제거시 발생한 Nitric Oxide
주명진,이도형,조혜진,이광주 인제대학교 1998 仁濟醫學 Vol.19 No.2
Nitric Oxide(NO)는 작고 비교적 불안정하고 독성이 있는 무기 가스로 L-arginine이 NO Synthase(NOS)에 의해 L-citrulline으로 전환될 때 생성되는 자유기이며 저농도에서는 생리적 기능을 하지만 고농도에서는 세포 독성을 일으킨다고 알려져 있다. 저자들은 상피세포에 손상을 주면 전반부 각막 간질세포에 세포사가 일어나는데, 이때 각막 간질세포의 세포사에 NO가 매개할 수 있다는 가정하에 실험적 각막 상피를 제거하였다. 실험결과 각막이 기계적으로 손상된 후 NO가 과다하게 생성되었고, NO 형성의 근원인 NOS 중에서 세포 독성이 강한 iNOS가 발현되는 것을 증명할 수 있었다. 따라서 각막 상피세포가 손상을 받았을 때 일어나는 각막 실질세포의 세포사 발생 기전에, 즉 각막 상피-각막 간질 상호 관계(epithelial-stromal interaction)에 NO가 매개할 수 있음을 시사한다고 할 수 있겠다. Apoptosis of the anterior stromal keratocytes has been demonstrated after corneal epithelial scraping wound. Nitric oxide(NO) is not only known to act as a neurotransmitter, but also act as a cytotoxic mediator when overproduced. Therefore, in this study, in order to detect the production of NO which may mediate the loss of keratocytes following epithelial injury, mechanical epithelial scraping in the rabbit eye was done. According to the time table, tear samples were collected with microcapillary tubes. NO production was assessed by measuring concentration of nitrite and nitrate. Immunohistochemical localization was performed on frozen sections from cryopreserved rat eyeball tissue using a polyclonal antibody against nitric oxide synthase(NOS). NO synthesis was increased after mechanical scraping, and reached a maximum value(0.60±0.09 μM/㎍ protein) at 12 hours after the treatment. There were statistically significant increases until 48hours postoperatively. Immunohistochemical staining of cornea showed that inducible and constitutive NOS were expressed in corneal stromal fibroblasts. These results suggest that mechanical scraping may induce NOS expression in cornea and NO produced by this enzyme may play an important role in mediating the stromal apoptosis after the removal of corneal epithelium.
주명진,박정제,곽형원,김재석,유진형 인제대학교 1994 仁濟醫學 Vol.15 No.3
백내장 수술시 채취한 수정체 전낭을 포함한 상피세포와 상피세포하의 조직을 전자현미경으로 관찰하여 전낭하 혼탁의 판(plague), 즉 임상에서 말하는 전극부 백내장 세포들의 성상과 그 기원을 추론하였다. The tissues of human cataract lens were obtained during capsulorhexis procedure to exam me the morphologic features of anterior subcapsular cataract. Abnormal fibril like materials and multi- parallel strand materials in lens capsule were observed, which were supposed to consisted of collagen originating from epithelial cells. The subcapsular plaque was demonstrated and it was composed by accumulation of degenerated epithelial cells with spindle shape and subepithelial lens fibers. Multilayered basement membrane like materials and fibrils were intermingled among these materials. Various sized vacuoles and empty spaces due to separation of cell membrane wherein functional complex present were observed. There are no proliferation of epithelial cells. We supposed that the central subcapsular opacity was made of accumulation of degenerated epithelial cells and subepithelial lens fibers, extracellular materials including multilayered basement membrane, fibrils and substance of cell break down, But further biochemical, histochemical and immunological investigations will be required to identify the substance or group of substance involved.
만성정신분열증 환자의 그림에 관한 현상학적 고찰 : 반복되는 주제를 중심으로
주명진 大韓神經精神醫學會 1979 신경정신의학 Vol.18 No.3
One frequently meets with the stereotypy in drawings during art therapy; the same motif is repeated several times in drawings. The author intended to reveal the characteristics of the stereotypy in this study. The subjects of the study include 12 chronic schizophrenics who revealed the stereotypy in their drawings. Control group include 20 chronic schizophrenics and non-schizophrenics who didn't show the stereotypy. The phenomenological characteristics of drawings of the study groups are as follows: 1. It showed more landscapes and still-lifes composed of plants and inanimate objects than the control groups, drawing. However, it revealed less human figures. 2. In terms of placement of the objects, it revealed more symmetricity than the control groups one. 3. It was composed of more cold colors than the contol groups' one. 4. In the structure of their drawings, it showed more baselines than the control groups' one. The characteristics of the study group compared with the control groups in views of clinical aspects are as follows: 1. Most of them were introvert in their premorbid personality. 2. Their ward activites and involvement to are therapy were passive in general.