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개 이자의 Somatostatin 양성세포의 발달에 관한 면역조직화학적 연구
장인엽,윤상필,정윤영,김종중,문정석,한길현,김영택,안계훈,선희매 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2
Cells immnunoreactive for somatostatin(SOM) and glucagon(GLU) were investigated in postnatal day 0, 7, 14, 28, 90, 180 and adult canine pancreas using light microscopic immunohistochemistry. Pancreatic SOM-immunoreactive cells were found in pancreatic acini, pancreatic duct, and pancreatic islet gradually. Pancreatic SOM-immunoreactive cells were more located in pancreatic parenchyme than pancreatic islet throughout pancreas by postnatal day 28. But there was change by the postnatal 3 months : pancreatic SOM-immunoreactive cells were more located in pancreatic islet than acini and duct. It is suggested that the number of the SOM-immunoreactive cells may not increase with age apparently. A small number of SOM-immunoreactive cells that showed GLU-immunoreactivity were also observed in canine pancreatic islet. The physiologic consequences of these changes remain to be determined. These results sugegest that SOM in the developing pancreas may relate to the secretion of pancreatic hormones.
개 시상하부의 Neuropeptides에 대한 면역조직화학적 연구
장인엽,정윤영,김종중,문정석,김준수,모근석,임용,유호진 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1
The distribution and localization of somatostatin(SOM), neuropeptide Y(NPY), vasopressin(VP), and oxytocin(OT) were studied with a immunohistochemical technique in the canine hypothalamus. The SOM-immunoreactive perikarya were concentrated in the anterior periventricular and the arcuate nuclei. Immunoreactive terminals were observed in the arcuate, the ventromedial hypothalamic, the suprachismatic nuclei and the external layer of the median emmence. A great number of NPY-immunoreactive cell bodies were found in the arcuate nucleus and NPY fibers were located throughout the hypothalamus with the highest concentration especially in the paraventricular (PVN) and the acuate nuclei. Moderately or densely stained fibers were also observed in the median eminence, the suprachiasmatic. the periventricular, the ventromedial hypothalamic, the dorsomedial hypothalamic and the medial preoptic nuclei, and the stria terminalis. Both VP-and OT-containing perikarya were found mainly in the PVN and supraoptic nucleus(SON). but a few were seen in the perifornical area, the lateral hypothalamus, the dorsal hypothalamic and the periventricular nucleus. The fibers originated from the PVN were projected to the SON via ventral or dorsal area of the fornix.
기니이픽 장관의 c-Kit 및 NK 1R 면역반응 세포구조에 대한 공초점 주사현미경적 연구
장인엽,김종중,문정석,김현곤,박찬국,전제열,전규배,조철희,유호진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1
Background and Objectives: Immunolabelling of interstitial Cajal(IC) cells in the intestinal wall has recently been developed by using a specific marker, the anti-c-Kit antibody. Substance-P is a well-known neurotransmitter in the gastro-intestinal tract. Since the gastro-intestinal wall structures have already been well documented in the guinea pig, immunohistochemistry was done for the c-Kit-positive IC network and substance-P receptor(NK1R) in an attempt to provide a morphological basis for the mechanism regulating gastro-intestinal movement. Materials and Methods: Cryosection and whole-mount preparations of guinea pig small intestine and colon were single and double immunolabelled using the anti-c-Kit and NK1R antibodies. Immunolabelled specimens were observed under a confocal laser scanning microscope. Results : According to a three dimensional reconstruction study, it was found that (1) the c-Kit-positive celluar networks were widely distributed in the intestinal wall, (2) c-Kit-positive celluar networks encircled the ganlion, with strands in reticular configurations, and (3) the c-Kit-positive cells showed colocalization with NK1R in circular muscle(CM), not myenteric plexus(MY). Conclusion: The charateristic profiles of IC containing c-Kit-positive celluar networks and the relationship between c-Kit-positive and NK1R-positive structures provide a morphological basis upon the mechanism regulating gastro-intestinal motility.
흰쥐 이자 Insulin과 Neuropeptide Y 분비세포의 발생에 관한 면역조직화학적 연구
장인엽,박태우,정윤영,김종중,문정석,김영택,안계훈,김남훈,선희매 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1
Cells immunoreactive to insulin(INS-IR) and neuropeptide Y(NPY-IR) were found in prenatal(E15, 17, 19) and postnatal(P0, 5, 10, 15, 30, adult) rat pancreas using immunohistochemistry. Pancreatic INS-IR and NPY-IR were detected by day E17. They were found initially in the interlobular pancreatic duct, intralobular pancreatic duct and pancreatic islet gradually. Especially, INS-IR was detected in the dorsal pancreatic bud by day E19. The INS-IR and NPY-IR patterns were nearly similiar to each other, and many cells revealed colocalization of INS and NPY until day P10. But there was a change between them by the day P15 ; central INS-IR cells and peripheral NPY-IR in the pancreatic islet. The number of pancreatic islets per square millimeter of pancreatic tissue were decreased, and the number of immunoreactive endocrine cells per islet was increased according to development. These results suggest NPY may play a role in the development of the pancreatic endocrine cells.
면역조절 한약제제를 이용한 당뇨병 합병증 질환치료제 개발 연구
장인엽 한국한의학연구원 2006 한국한의학연구원 연구보고서 Vol.- No.-
To date diabetic nephropathy is believed to be the consequence of progressive T cell-mediated autoimmune destruction of kidney cells. The generation of free radicals in target cells has been also implicated as one of the various mechanisms involved in the destruction of kidney cells. The objectives of this research are to screen oriental medicinal herbs for the treatment of diabetic nephropathy and investigate mechanism by which high glucose destruct kidney cells. To develop drugs for the treatment of diabetic nephropathy, we first screened for the activities of nitric oxide (NO) synthesis from oriental medicinal herbs. NO participates in the inflammatory response of macrophages and plays important roles in the destruction of pancreatic beta cells and kidney cells (Hibbs et al., 1987). The positive fractions were then further analyzed for the NO inhibition by dose-response study. We screened 380 herbs and confirmed 13 positive fractions. We investigated the effect of the combined extracts (KIOM-79) isolated from Magnolia officinalis, Pueraria lcbata, Glycyrrhiza uralensis, and Euphorbia pekinensis on the production of NO. To further investigate the mechanism by which KIOM-79 inhibits the expression of iNOS gene, we assessed the effects of KIOM-79 on the activation of NF-кB. The present studies demonstrate that KIOM-79 inhibits iNOS gene expression through the inhibition of NF-KB. KIOM-lO also inhibited iNOS gene expression by blocking NF-KB and p38 kinase activation. We found that high glucose demage kidney cells and inhibits the activities of membrane transporters. We also found that NF-кB and protein kinase C are potential targets for high glucose treatment. Successive results of this project will contribute to studies involved in the pathogenesis of diabetic nephropathy, the development of new anti-diabetic drugs, and the action mechanisms of the drugs. The immunosupressive medicinal herbs selected by the screening will be used to test the possibilities for drugs against diseases (other autoimmune diseases, skin aging, or cancer) as well as insulin-dependent Diabetes mellitus. Products from the project such as, screening system and positive fractions of the herbs can be used to study medicine, pharmacology, and biology.