난소의 호산성 투명세포암종 - 1예의 보고 -

하창원,고재수,명나혜,조경자,박상윤,김미경,장자준 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.5

요관석의 요관경하배석술 후 요관부목의 선별적 유치

이상훈,윤하나,심봉석,이시내 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

Purpose : Ureteral stent is commonly used after ureterscopic stone removal(URS). However, there is no definitive clinical criteria in inserting the ureteral stent after URS. Patients often complain of bladder irritative symptoms, hematuria. flank pain and additive endoscopic prodedure is neces-sary to remove the ureteral stent. Therefore, we studied the clinical epicacy of selective ureteral stenting only when it is required. Materials and Methods : Of the patients who was taken URS, the following patients were exclu-ded from stent insertion ; 1) Patients who have mild hydronephrosis or symptoms less than 1week. 2) Patients who were able to insert the ureteroscope through the ureteral orifice without difficulty. 3) Patients who hardly have ureteral mucosal damage seen through the ureteroscopic field after the removal of ureteral stone. 4) Patients who did not need the additional lithoclast during the remo-val of ureteral stone. Other than the above patients and those with stones above the mid-ureter, a 6Fr. ureteral stent was inserted and kept for 3weeks. There were 29 unstented patients and 38 stented patients. Results : The average age of the non-stenting and stenting group were 37.5years old and 39.7years old and the average stone size were 0.68cm and 0.80cm. The average operation time was 3 minutes in the non-stenting and 8minutes(including ureteral stent insertion time) in the stenting group. Hos-pital stay was 3days in both groups. Intermittent gross hematuria and irritative symptoms of the bladder was shown for 3weeks after operation in most of the patients(32cases, 84.2%) of the sten-ting group while those in the unstenting group, the symptoms were relieved after2days after operation. Although mild flank pain was shown in few patients(4cases, 13.8%) of the non-stenting group, severe pain requiring intravenous analgesics was not seen in both groups. There were no specific findings in the intravenous urography which was done at 6weeks after operation, and no hematuria and pyuria was found in both groups. Conclusion : The selective placement of ureteral stent following the ureteroscopic removal of stone did not bring specfic complications. We believe that ureteral stent insertion will minimize the inconvenience especially in patients who need to return to their everyday living immediately after operation. Thus ureteral stent insertion should be used in limited situations. 요관경하배석술 후 많은 경우에 있어서 요관부목을 유치하고 잇지만 선별적으로 판단하여 설치할 경우 특별한 문제점이 없었으며, 수술 수 바로 일상생활에 복귀하여야 하는 환자들의 불편함을 최소화할 수 잇을 것으로 생각되며, 요관부목 설치는 제한적인 상황하에서 시행되어야 할 것으로 사료된다.

영구치 치수 기질세포를 이용한 연골 분화 및 분화 시기에 따른 형태학적 변화

정주령,김하나,박열,김민정,오영주,신수정,최윤정,김경호 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.1

Objectives: The aim was to confirm the stem cell-like properties of the dental pulp stromal cells and to evaluate the morphologic changes during in vitro chondrogenesis. Materials and Methods: Stromal cells were outgrown from the dental pulp tissue of the premolars. Surface markers were investigated and cell proliferation rate was compared to other mesenchymal stem cells. Multipotency of the pulp cells was confirmed by inducing osteogenesis, adipogenesis and chondrogenesis. The morphologic changes in the chondrogenic pellet during the 21 day of induction were evaluated under light microscope and transmission electron microscope. TUNEL assay was used to evaluate apoptosis within the chondrogenic pellets. Results: Pulp cells were CD90, 105 positive and CD31, 34 negative. They showed similar proliferation rate to other stem cells. Pulp cells differentiated to osteogenic, adipogenic and chondrogenic tissues. During chondrogenesis, 3-dimensional pellet was created with multi-layers, hypertrophic chondrocyte-like cells and cartilage-like extracellular matrix. However, cell morphology became irregular and apoptotic cells were increased after 7 day of chondrogenic induction. Conclusions: Pulp cells indicated mesenchymal stem cell-like characteristics. During the in vitro chondrogenesis, cellular activity was superior during the earlier phase (within 7 day) of differentiation.

Gintonin facilitates catecholamine secretion from the perfused adrenal medulla

Seung-Yeol Na,Ki-Hwan Kim,Mi-Sung Choi,Kang-Su Ha,Dong-Yoon Lim 대한생리학회-대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.6

The present study was designed to investigate the characteristics of gintonin, one of components isolated from Korean Ginseng on secretion of catecholamines (CA) from the isolated perfused model of rat adrenal gland and to clarify its mechanism of action. Gintonin (1 to 30 μg/ml), perfused into an adrenal vein, markedly increased the CA secretion from the perfused rat adrenal medulla in a dose-dependent fashion. The gintonin-evoked CA secretion was greatly inhibited in the presence of chlorisondamine (1 μM, an autonomic ganglionic bloker), pirenzepine (2 μM, a muscarinic M₁ receptor antagonist), Ki14625 (10 μM, an LPA_1/3 receptor antagonist), amiloride (1 mM, an inhibitor of Na⁺/Ca²⁺ exchanger), a nicardipine (1 μM, a voltage-dependent Ca²⁺ channel blocker), TMB-8 (1 μM, an intracellular Ca²⁺antagonist), and perfusion of Ca²⁺-free Krebs solution with 5mM EGTA (a Ca²⁺chelater), while was not affected by sodium nitroprusside (100 μM, a nitrosovasodialtor). Interestingly, LPA (0.3~3 μM, an LPA receptor agonist) also dose-dependently enhanced the CA secretion from the adrenal medulla, but this facilitatory effect of LPA was greatly inhibited in the presence of Ki 14625 (10 μM). Moreover, acetylcholine (AC)-evoked CA secretion was greatly potentiated during the perfusion of gintonin (3 μg/ml). Taken together, these results demonstrate the first evidence that gintonin increases the CA secretion from the perfused rat adrenal medulla in a dose-dependent fashion. This facilitatory effect of gintonin seems to be associated with activation of LPA- and cholinergic-receptors, which are relevant to the cytoplasmic Ca²⁺ increase by stimulation of the Ca²⁺ influx as well as by the inhibition of Ca²⁺ uptake into the cytoplasmic Ca²⁺ stores, without the increased nitric oxide (NO). Based on these results, it is thought that gintonin, one of ginseng components, can elevate the CA secretion from adrenal medulla by regulating the Ca²⁺ mobilization for exocytosis, suggesting facilitation of cardiovascular system. Also, these findings show that gintonin might be at least one of ginseng-induced hypertensive components.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

Studies on improvement of blood glucose and diabetic complications by Xanthii fructus and its chemical constituents

Ha Na Yoon,Min Young Lee,Jin-Kyu Kim,Hong-Won Suh,Il-Jun Kang,Soon Sung Lim 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

Inhibitory effect of the compounds isolated from Xanthii fructus on α-Glucosidase

Ha Na Yoon,Min Young Lee,Jin-Kyu Kim,Hong-Won Suh,Il-Jun Kang,Soon Sung Lim 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

The Korean Society of Gastroenterology & SIDDS 2031 : Slide Session ;K-LG-02 : Lower GI Tract ; Acai Reduces Azoxymethane/Dextran Sulfate Sodium- Induced Mouse Colon Carcinogenesis

( Yoon Jin Choi ),( Na Young Kim ),( Yoon Jeong Choi ),( Ryoung Hee Nam ),( Ji Hyung Seo ),( Seonmin Lee ),( Mi So Kim ),( Min Hee Ham ),( Ha Na Lee ),( Kichul Yoon ),( Cheol Min Shin ),( Dong Ho Lee 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: Acai is well-known for its anti-oxidative action. To evaluate protective effects of acai powder (AP) intake on azoxymethane (AOM)/dextran sulfate sodium (DSS)-induced colon tumors in an experimental mice model. Methods: Six groups of 5-week-old ICR mice were used. Carcinogen groups; 24 micewere injected intraperitoneally with 10mg/kg of AOM once and orally administered with 2.5% of DSS for 7 days from a week after the injection. Bothe control and AOM-treated groups were divided in to three groups, respectively (G1-G6); fed with standard diet, a diet containing 2.5% or 5.0% of AP for the rest of 14 weeks after the administration of DSS. Sixteen weeks after AOM injection, all groups were sacrificedfor histopathology analysis and the colon tumor assay. Key molecules of infi ammation and proliferation pathway, such as IL-1ß, IL-6, TNF-a, cyclooxygenase-2 (COX-2), myeloperoxidase (MPO) and proliferating cell nuclear antigen (PCNA) were assessed frommice colonic mucosa. Results: Eight (100%), 6 (75%) and 4 (50%) mice in each AOM-treated group (G4-G6) developed cancers (P trend = 0.024). Among AOM-treated mice, signifi cant reduction in tumor multiplicity and tumor size were observed in the both groups fed with AP compared to the standard diet group (multiplicity: 10.1 ± 2.3 vs. 2.8 ± 0.9 and 2.6 ± 0.8 P = 0.025, P = 0.023; size: 5.8 ± 0.8 vs. 2.5 ± 0.8 and 2.4 ± 1.0, P = 0.025, P = 0.016). Also, significant reduction in COX-2 expression in the AOM-treated group with AP and inhibition of IL-1ß, IL-6, TNF-a, MPO and PCNA expressions in the AOM-treated groups with AP in a dose-dependent manner (all P < 0.05). Conclusions: Acai reduced the incidence, multiplicity and size of AOM/DSS-inducedtumor in mice. Acai may have a potential to prevent colon carcinogenesis via anti-in- fi ammatory and anti-proliferative properties.

Synthesis and Applications of Noble Metal and Metal Silicide and Germanide 1-Dimensional Nanostructures

Yoon, Ha-Na,Yoo, Young-Dong,Seo, Kwan-Yong,In, June-Ho,Kim, Bong-Soo Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.9

This review covers recent developments in our group regarding the synthesis, characterization and applications of single-crystalline one-dimensional nanostructures based on a wide range of material systems including noble metals, metal silicides and metal germanides. For the single-crystalline one-dimensional nanostructures growth, we have employed chemical vapor transport approach without using any catalysts, capping reagents, and templates because of its simplicity and wide applicability. Au, Pd, and Pt nanowires are epitaxially grown on various substrates, in which the nanowires grow from seed crystals by the correlations of the geometry and orientation of seed crystals with those of as-grown nanowires. We also present the synthesis of numerous metal silicide and germanide 1D nanostructures. By simply varying reaction conditions, furthermore, nanowires of metastable phase, such as $Fe_5Si_3$ and $Co_3Si$, and composition tuned cobalt silicides (CoSi, $Co_2Si$, $Co_3Si$) and iron germanides ($Fe_{1.3}Ge$ and $Fe_3Ge$) nanowires are synthesized. Such developments can be utilized as advanced platforms or building blocks for a wide range of applications such as plasmonics, sensings, nanoelectronics, and spintronics.

Inhibitory effect of the compounds isolated from Xanthii fructus on aldose reductase

Ha Na Yoon,Min Young Lee,Jin-Kyu Kim,Hong-Won Suh,Il-Jun Kang,Soon Sung Lim 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10