녹차추출물에 의한 식중독 원인균인 장내세균 Plesiomonas shigelloides의 세포반응

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.2

The aim of this work was to investigate the cellular responses in the food-poisoning bacterium, Plesimonas shigelloids in response to tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentrations in exponentially growing culture of P. shigelloides. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DnaK and anti-GroEL monoclonal antibodies. Survival of P. shigelloides with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000 ㎍/㎖ TPP within 2 hours of incubation. Scanning electron microscopy for the cells treated with 10,000 ㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

파종시기가 다른 일미찰의 등숙 중 호화 특성 비교

김미정, 박효진, 이유영, 김선림, 김정태, 우관식, 권영업, 정일민 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

This study was conducted to investigate the pasting properties of waxy corn ‘Ilmichal’ during ripening with different sowing date. Starch content of Ilmichal was increased as matured (p<0.05). Analysis of pasting properties with waxy corn starch using a rapid visco analyser (RVA) showed linear changes in peak viscosity, trough viscosity, final viscosity, breakdown and consistency according to ripening. There were significantly positive correlation between starch content of Ilmichal and peak viscosity (0.39*), trough viscosity (0.58***), final viscosity (0.58***), and consistency (0.57**). Differential scanning calorimerty (DSC). The enthalpy of gelatinization was increased as ripening. But onset and peak temperature were decreased. There were significantly positive correlation between starch content of Ilmichal and onset temperature (0.44*), peak temperature (0.38*), and completion temperature (0.59***), on the other hand, crude protein content presented significantly negative correlation between completion temperature (-0.41*).

Tea polyphenol에 노출된 식중독 원인세균 Listeria monocytogenes의 살균

유미옥,천재우,오계헌 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The aim of this work was to investigate the killing effect of the food-poisoning bacterium, Listeria monocytogenes exposed by tea polyphenol (TPP) extracted from green tea. The stress shock proteins, which contribute to the resistance of the cytotoxic effect of TPP, were induced at different TPP concentration in exponentially growing cultures of L. monocygenes. This response involved the induction of a 70-kDa DnaK and a 60-kDa GroEL monoclonal antibodies, characterized by SDS-PAGE and Western blot by the use of the anti-DanK and anti-GroEL monoclonal antibodies. Survival of L.monocytogenes with time in the presence of different concentrations of TPP was monitored, and viable counts paralleled the induction of the stress shock proteins in this strain. This strain killed in the concentration of 1,000㎍/㎖ TPP within 4 hours of incubation. Scanning electron microscopy for the cells treated with 1,000㎍/㎖ for 12 hours showed the presence of perforations and irregular rod shaped with wrinkled surfaces.

소아에서 성장호르몬 결핍증 진단을 위한 혈중 IGF-1과 IGFBP-3의 절단값

손미란,안영선,정우영,유병철 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Background : It is essential to have reference criteria for the diagnosis of growth hormone deficiency in children. The objective of study was to decide the cut-off value for Insulin-like Growth Factor-Ⅰ(IGF-Ⅰ) and Insulin-like Growth Factor Binding Protein-3(IGFBP-3) for diagnosis of growth hormone deficiency. Methods : We studied the serum level of IGF-Ⅰ and IGFBP-3 in 231 subjects(131 boys and 100 girls) with idiopathic short stature(ISS) and 37 subjects(23 boys and 14 girls) with complete or partial growth hormone deficiency(GHD). IGF-Ⅰ were measured by radioimmunoassay(RIA) method and IGFBP-3 were measured by immnoradiometric assay(IRMA) method. We conducted chi-square test, Fisher's exact test, student t-test, receiver operating characteristic(ROC) curve and discriminant anaylsis using MedCalc and SPSS Program. Results : The ROC curves showed that the best IGF-Ⅰ and IGFBP-3 cut-off value. Cut-off value of serum IGF-Ⅰ concentration was 346ng/mL(sensitivity 86.49%, specificity 85.71%) and of serum IGFBP-3 concentration was 3196ng/mL(sensitivity 81.82%, specificity 81.66%). If discriminant value (Z=0.442+0.661×IGF-Ⅰ+0.469×IGFBP-3) was below -1.47, the subject is discriminated GHD respectively. Conclusion : Our study results support that the measurement of serum IGF-Ⅰ and IGFBP-3 concentration might be an essential supplementary tools in the diagnostic evaluation of children with GHD.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

레진 시멘트를 이용한 레진 파이버 강화 레진포스트의 치근 상아질에 대한 미세인장결합강도

김진우,유미경,이세준,이광원 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.1

Object The purpose of this study were to evaluate the microtensile bond strength of resin fiber reinforced post to radicular dentin using resin cement according to various dentin surface treatment and to observe the interface between post and root dentin under SEM. Material and Method A total 16 extracted human single rooted teeth were used. A lingual access was made using a #245 carbide bur in a high-speed handpiece with copious air water spray. The post space was mechanically enlarged using H-file(up to #60) and Gates Glidden bures(#3). This was followed by refining of the canal space using the calbrating drill set provided in ER Dentinpost(GEBR, BRASSELER GmbH&Co. KG). The 16 teeth were randomly distrubuted into 4 group of 4 teeth. Group 1 teeth had their post space prepared using 10% phosphoric acid as root canal surface treatment agent during 20s. The canal was then rinsed with saline and dried with paper point. Group 2 teeth had their post space prepared using 3% NaOCI as root canal surface treatment agent during 30min. The canal was then rinsed with saline and dried with paper point. Group 3 teeth had their post space prepared using 17% EDTA as root canal surface treatment agent during 1min. The canal was then rinsed with saline and dried with paper point. Group 4 teeth had their post space prepared using 17% EDTA as root canal surface treatment agent during 1 min. After rinsing with saline, the canal was rinced 10ml of 3% NaOCI for 30min. After drying with paper point, the post(ER Dentinpost, GEBR, BRASSELER GmbH&Co. KG) was placed in the treated canals using resin cement. Once the canal was filled with resin cement(Super bond C&B sunmedical co. Ltd.), a lentulo was inserted to the depth of the canal to ensure proper coating of the root canal wall. After 24 hours, acrylic resin blocks(10·15·50mm) were made. The resin block was serially sectioned vertically into stick of 1·1mm. Twenty sticks were prepared from each group. After that, tensile bond strengths for each stick was measured with Microtensile Tester. Failure pattern of the specimen at the interface between post and dentin were observed under SEM. Results 1. Tensile bond strengths(meen±SD) were expressed with ascending order as follows ; group 4, 12.52±6.60 ; group 1, 7.63±5.83 ; group 2, 4.13±2.31 ; group 3, 3.31±1.44. 2. Tensile bond strengths of Group 4 treated with 17% EDTA +3%NaOCI were significant higher than those of group 1, 2 and 3 (p<0.05). 3. Tensile bond strengths of Group 1 treated with 10% phosphoric acid were significant higher than those of group 2 (p<0.05). Tensile bond strengths of Group 4 treated with 17% EDTA +3% NaOCI was significant higher than those of other groups.

좌측흉수로 발현된 Boerhaave 증후군 1예

이준,변유미,조민근,조주연,원경준,김영대,박찬국,김만우,유재근,서홍주,이석기 朝鮮大學校 附設 醫學硏究所 2008 The Medical Journal of Chosun University Vol.33 No.2

구토 후 발생한 토혈을 주소로 내원한 환자에서 좌측 늑막 삼출 동반 시 식도 파열의 가능성을 고려하여 흉수 천자 및 지속적인 흉부 단순 촬영의 추적관찰이 필요하며 식도 파열이 배제된 후 환자의 생체 징후 및 토혈 양을 파악하여 내시경 검사 시기를 신중히 결정하는 것이 좋을 것으로 생각된다. 본 증례는 토혈을 주소로 내원하여 Mallory-Weiss 증후군 의심 하에 내시경을 통한 치료 시행 후 추적 관찰에 의해 진단된 Boerhaave 증후군을 경험하였기에 보고하는 바이다. Boerhaave's syndrome is a spontaneous perforation of the esophagus most commonly resulting from a sudden increase in intraesophageal pressure combined with negative intrathoracic pressure caused by straining or vomiting. Surgery should generally be performed within 24 hours because mortality approached 30 to 50 percent in which surgery was delayed. Therefore, early diagnosis and prompt surgical repair are critical for survival. We have experienced a case of Boerhaave's syndrome in a patient presenting with hematemesis with left pleural effusion without any signs or symptoms of pneumomediastinum or pneumothorax.

아메바성 대장염과 연관된 Henoch-Scho¨nlein Purpura 1예

장재현,원경준,이준,변유미,이재광,김영훈,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Henoch-SchonIein purpura is a common form of vasculitis primarily involving the skin, the gastrointestinal tract, the joints, the kidneys, and the nervous system. The etiological investigations are essential, because triggering factors are found in approximately half the patients such as viral, bacterial and parasitic infections, drugs, toxins, systemic diseases and cancer. But it's the correlation between Henoch-SchonIein purpura and Entamoeba histolytica has been rarely reported. We report a 29-years-old woman with Henoch-SchoIein purpura complicated by the lower gastrointestinal bleeding caused by the amoebic colitis. She complained of cramping abdominal pain, lower extremity purpura and hematochezia. Cysts and trophozoites of Entamoeba histolytica were detected in her stool. Skin biopsy demonstrated the leukocytoclastic vasculitis. She was administered metronidazole 1,500㎎ a day for the treatment of amebic colitis. Her hematochezia was improved, but the abdominal pain and purpura were not. The subsequent corticosteroid therapy improved her symptoms.

제 1형 신경섬유종증 환자에서 발생한 위장관 간질종양 1예

원경준,이준,변유미,조민근,한경택,김원,홍란,임성철,김경종,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract, but this tumor is rarely seen in association with type l neurofibromatosis (NFl) Generally, the majority of GISTs are isolated neoplasm and they are sporadically found in the stomach, but the GISTs in NFl patients are usually multiple and usually found in the small intestine. We report a case of multiple GISTs in the jejunum of a 63-year-old woman diagnosed as NFl accompanied by complication of gastrointestinal bleeding, In this case, Patient had multiple cafe-au lait spots and neurofibroma on skin and had freckling on axilla and groin, and then, we made a diagnosis of NFl. Gastrointestinal bleeding is controlled by resection of multiple GISTs. Generally, only 3-5% of all gastrointestinal bleeding comes from the small bowel. Causes of small intestinal bleeding are angiodysplasia, neoplasm, NSAIDs induced ulcer etc. but, If patients have NFl accompanied by complication of gastrointestinal bleeding, GISTs on small bowel must be considered.

제2형 당뇨병 동물모델인 OLETF 쥐에서 장기간 라미프릴 투여가 내당능 및 췌도 베타세포에 미치는 효과

고승현,윤건호,김명미,안유배,송기호,유순집,손현식,차봉연,이광우,손호영,강성구 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.6

연구배경:최근 소개된 HOPE(Heart Outcomes Prevention Evaluation)study의 결과에 의하면, 심혈관질환이나 이에 대한 위험인자가 1가지 이상인 9,541명을 대상으로 안지오텐신 전환효소 억제제의 일종인 라미프릴을 투여하여 사망율과 뇌졸증, 심부전, 당뇨병 합병증이 개선되었을 뿐 아니라 다른 항고혈압제에 비해 새롭게 당뇨병으로 진단되는 환자가 줄었음을 보고한바 있다. 이에 저자들은 라미프릴(ramipril)을 제2형 당뇨병 동물모델에 장기간 투여함으로써 당뇨병을 예방하거나 고혈당 상태 또는 인슐린 감수성을 개선시킬수 있는지를 알아보고자 하였다. 방법:생후 24주된 체중 400∼450g의 OLETF와 LETO 쥐를 대상으로 6개월간 라미프릴 경구투여 하였고 혈압, 24시간 단백뇨 및 인슐린 내성검사를 시행하였다. 6개월 이후로는 8주간 투여군과 대조군의 체중을 비슷하게 맞추어 내당능 상태를 평가하였고 이후 다시 8주간 고농도 포도당을 투여하였다. 경구당부하검사를 시행한 후 희생시켜 조직표본을 만들어 guinea pig anti­human insulin 항체로 염색후 DAB로 발색하여 point count 법으로 베타세포를 정량하였고, 면역염색으로 췌도의 변화와 섬유화의 정도를 관찰하였다. 결과:라미프릴을 6개월간 투여하였을 때 OLETF 쥐에서 1)체중 증가가 적었으며 2)경구 당부하검사시 포도당하 면적은 의미있게 감소하였고 3)인슐린 내성검사상 Kitt가 증가되는 경향을 보였으며 4)수축기, 이완기 혈압과 24시간 단백뇨양이 의미있게 감소하였고 5)체중을 맞추면 내당능 상태가 비슷해졌다가 고농도 포도당 주입시 다시 포도당하 면적이 대조군에서 더 증가되었다. 라미프릴 투여군에서 췌도변형 및 파괴와 탈과립, 췌도내 섬유화가 감소하였다. 결론:본 실험에서는 인슐린 비의존성 당뇨병 동물 모델인 OLETF 쥐에서 당뇨병 발생 이전 단계부터 장기간 라미프릴을 투여할 경우 체중증가가 적었고, 투여군과 대조군의 체중을 맞추었을 때는 내당능상태의 차이가 없아가 고혈당 투여시 다시 투여군과 대조군에서 의미있는 차이를 보였다. 라미프릴 투여가 베타세포의 기능면에서 이로운 효과를 보일 것으로 생각되었다. 따라서 비만한 환자에서 안지오텐신 전환효소 억제제의 장기투여는 단백뇨 감소, 혈압강하 효과 이외에 체중증가의 억제 및 당뇨병 발생을 억제할 가능성을 시사한다. Background : In a Heart Outcomes Prevention Evaluation HOPE study, ramipril, a long-acting angiotensin-converting enzyme (ACE) inhibitor, significantly reduced the death rates the number of myocardial infarctions, strokes, heart failure as well as the risk of complications related to diabetes and of diabetes itself. However, it is known that ACE inhibitors improve glucose tolerance or insulin sensitivity or reduce the incidence of diabetes. Methods : 24 week-old OLETF (Otsuka Long Evans Tokushima Fatty) rats weighing 400 ~ 450 g were used in this study. 4 groups of rats were examined in parallel for 40 weeks. The OLETF rats were randomized for treatment with an aqueous solution of ramipril (5 mg/Kg) daily [OL (RMP), n=10)] and with saline [OL (CON), n=10)]. The LETO rats were also randomized in the same was as the OLETF rats (LT(RMP), n=10, LT (CON), n=10). The blood glucose level, body weight, systolic and diastolic blood pressure was assessed every month. At 3 and 6 months, the 24hrs urinary protein concentration was measured, and as insulin tolerance test and oral glucose tolerance test were conducted in all experimental groups. After 6 months, the body·weight was matched for 2 months in each corresponding group. Subsequently, a 15% sucrose loading was done for 2 months. After the glucose tolerance test, the pancreas was excised and immuno histochemical staining was conducted for insulin to quantify the beta cell mass by a point-counting method. In addition, the islet morphology was evaluated in the pancreas. Results : Ramipril treatment for a period of 6 months improved the 2hr blood glucose level, the area under the glucose curve in the oral glucose tolerance test, insulin sensitivity in addition to lowering significantly systolic and diastolic blood pressure and 24hrs urinary protein level significantly in OLETF rats. Of note, a lower weight gain was observed in both the ramipril-treated animals at 6 months. After weight matching, the AUC g and 2hr blood glucose level values were similar between the corresponding groups, but a 15% sucrose loading worsened the AUC g value. Histologically, the islets were less disorganized and the extent of fibrosis was lower in the ramipril-treated OLETF rats in the trichrome stain. Conclusion : Long-term treatment of ramipril, a long acting angiotensin-converting enzyme inhibitor may be useful for suppressing weight gain and proteinuria in addition to having a protective effect on the islet to harmful stimuli such as hyperglycemia (J Kor Diabetes Asso 25:469~482, 2001).