누룩 투입시기에 따른 막걸리의 품질특성

천재우,조춘봉 사단법인 한국벤처혁신학회 2018 벤처혁신연구 Vol.1 No.2

본 연구와 실험은 막걸리의 양조 과정에서 1단계·2단계 담금으로 밑술을 만들고, 3단계 담금으로 막걸리를 만들고, 누룩의 물리·화학적 특성에 따른 막걸리의 최적 조건을 위하여 설정하였으며, 물리·화학적 특성의 변화를 확인하고 누룩 입력 시점을 기준으로 전통적 막걸리의 특성 검사를 시행하였다. 1단계·2단계 밑술의 물리·화학적 특성을 시험한 결과, pH와 산도는 step-1 담금으로 배양 한 날에 크게 감소하였고 배양 2 일까지는 추세가 지속되었다. 또한 아로마, 산도, 단맛 및 입안에서의 감촉 정도를 분석하기 위하여 관능검사를 실시하였으며, 그 결과로서 아로마는 M3단계에서 4.47로 가장 높았고, 산도는 M3단계에서 3.65로 가장 높았으며, 단맛은 M1단계에서 3.88, 입안에서의 감촉으로는 M1단계에서 3.59으로 가장 높았으며. 아로마와 맛을 포함한 전반적인 선호도는 M3에서 3.71로 가장 높게 나타났다. The research and the experiments were on physicochemical characteristics of Makgeolli based on input timing of Nuruk in the brewing processes, i.e., making mitsul with step-1/step-2 mashing, and making Takju with step-3 mashing, to establish the optimal conditions required for Makgeolli by checking the change of physicochemical characteristics and by inspecting culture characteristics based on input timing of Nuruk. When testing the physicochemical characteristics of mitsul(step-1/step-2), pH and acidity were reduced drastically on Day 1 of culture with step-1 mashing and the trend continued until Day 2 of culture, while there was no large change since then. In the sensory test, the aroma was the highest in M3 at 4.47, the acerbic taste was the highest in M3 at 3.65, the sweet taste was the highest in M1 at 3.88, and the feel in the mouth was the highest in M1 at 3.59. Overall preference encompassing aroma and taste was the highest in M3 at 3.71.

토양에서 분리된 Acrylamide 분해 세균 JK-7의 분리 및 특성

천재우,오계현 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2

The feasibility of using pure culture for acrylamide degradation, with the ultimate aim of application for biological treatment process, was explored. The present study reports on the test cultures which were developed to grow aerobically with acrylamide as the sole source carbon and nitrogen. A bacterial isolate, strain JK-7 was isolated from paddy soil samples, Strain JK-7 could degrade 50 mM acrylamide completely within 72 hours of incubation. Major intermediates resulting from acrylamide degradation were not detected with the HPLC methodology except acrylic acid which appeared to accumulate transiently in the growth medium. Initial pH 7.2 of the cultures became to increase 8.4 at the end of incubation. When JK-7 cells were grown at over 100 mM acrylamide, there was a pause of cell growth, resulting in a reduction in the rates of acrylamide degradation. Survival test revealed that cells exposed to low concentrations of acrylamide enable to the strain JK-7 to survive at a lethal concentration of 100 mM acrylamide. The relationships between the acrylamide degradation by JK-7 and several relevant physicochemical environmental parameters were examained. The effect of supplemented carbons(e.g., glucose, fructose, citrate, succinate) on the acrylamide degradation by the test culture of JK-7 was evaluared. The results indicated that the addition of carbons accelerated the bacterial growth and acrylamide degradation compared to in the absence of supplemented carbons. The effect of supplemented nitrogens on the degradation was monitored. Increasing concentrations of yeast extract resulted in higher growth yield, based on the turbidity measurement, and complete degradation of acrylamide. However, acrylamide degradation was essentially uninfluenced by the addition of (NH_(4))_(2)So_(4),NH_(4)Cl, or urea. The bacterium was identified as belonging to the genus Pseudomonas in the basis of use BIOLOG test, and designated as Pseudomonas sp. JK-7

해양에서 분리한 Lantobacillus sp. JK-8에 의한 질병원인 세균의 살균효과

천재우,마채우,오계헌 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

The purpose of this work was to investigate for killing effect of disease-causing bacteria by Lactobacillus sp. JK-8 isolated from marine environment. Initially, a bacterial culture, strain JK-8 was developed to grow no MRS media. The bacterium was identified as genus Lactobacillus on the basis of BIOLOG test, and designated as Lactobacillus sp. JK-8. The intial pH 7 of the cultures became to decrease pH 3.85 at the end of incubaction according to the growth of Lactobacillus sp. JK-8. The antibacterial activity using plate diffusion method against target bacteria was determined with 5-fold concentrate of cell-free culture supernatant. Excellent killing effect of target bacteria was achieved. The inhibition zone obtained with culture supernatant were in between 14 mm and 20 mm. Killing rate of pathogenic bacteria was examined with cell-free concentrated supernatants. All bacteria tested in this work completely killed within 3 hrs of incubaction.

새우양식장에서 분리한 Lactobacillus sp. JK-8의 생리적 특성

천재우,마채우,오계헌,Chun Jae-Woo,Ma Chae-Woo,Oh Kye-Heon 한국미생물학회 2005 미생물학회지 Vol.41 No.1

이 연구는 새우양식장에서 분리된 Lactobacillus sp. JK-8의 생리적 특성을 규명하기 위하여 실시되었다. 균주 JK-8을 MRS 배지에서 배양하였고, 형태 및 생리학적 특성에 대하여 조사하였으며, BIOLOG시험을 통하여 이 세균은 Lactobacillus속으로 동정되었다. 배양기간 동안에 균주 JK-8의 생장과 PH변화를 조사하였으며, 생성되는 유기산(lactic acid와 acetic acid)은 JK-8 배양의 생장과 비례하는 것을 확인하였다. Lactic acid와 acetic acid의 농도는 각각 192.8 mM과 43.6 mM이었으며, 초기 pH 7.0은 배양기간동안 3.8로 감소하였다. 8가지 대상 세균에 대하여 5배로 농축된 배양 상등액에 처리하여 살균효과를 조사하였으며, 이 연구에서 모든 대상 세균들은 배양3시간 이내에 완전히 살균되었다. pH조절을 하지 안은 JK-8 배양에서 대상세균들에 대한 항균효과가 있는 것이 관찰되었으나, pH가 조절된 배양에서는 거의 관찰되지 않았다. 대사산물로서 lactic acid와 acetic acid의 분리하기 위하여 HPLC를 사용하였으며, GC-MS를 이용하여 이들 대사산물을 확인하였다. The purpose of this work was to investigate the physiological characteristics of Lactobacillus sp. JK-8 isolated from a shrimp aquaculture pond. The strain JK-8 was grown on MRS media, and morphological and physiological characteristics of the strain were examined. The bacterium was identified as a strain of the genus Lactobacillus on the basis of BIOLOG test. Strain JK-8 produced both lactic acid and acetic acid, which were responsible for the pH decrease during growth. Concentrations of lactic acid and acetic acid increased to 192.8 mM and 43.6 mM, respectively, and the initial pH 7.0 of the cultures decreased to 3.8 at the end of incubaction. The bacteriocidal effect against eight target bacteria was examined with 5-fold concentrated culture supernatants. All bacteria tested in this work were completely killed within 3 hrs after treatment with the culture supernatant. The bacteriocidal effects were clearly observed, only when the pH of the culture supernatants were not adjusted. HPLC was used to reslove lactic acid and acetic acid in the culture solution, and GC-MS was used to verify the metabolites.

노인 사회활동 지원사업 참여가 노후 삶에 미치는 영향 : 사회활동 지원사업 참여 전, 참여 후 분석을 중심으로

천재우 사단법인 한국벤처혁신학회 2018 벤처혁신연구 Vol.1 No.2

우리나라의 평균수명이 1970년 평균 62.3세, 2016년 82.3세로 평균수명이 20년 이상 연장되면서 고령화 사회를 걸쳐 초고령화 시대에 접어들 것으로 예상되어 진다. 노인의 4苦인 빈곤, 고독, 무의, 질환의 문제는 사회문제로 대두되어 지고 있다. 노인복지정책으로 2004년부터 노인 일자리사업(사회활동 지원사업)을 진행하였다. 목적은 첫째, 일을 통한 적극적 사회참여, 소득보충 및 건강증진 등으로 노인문제 예방 및 사회적 비용 절감, 둘째, 다양한 일자리를 통한 건강한 노후생활 영위를 목적으로 한다. 본 연구에서는 노인 일자리사업(사회활동 지원사업) 참여 전과 참여 후의 설문조사를 통하여 노인 일자리사업(사회활동 지원사업)이 사업 목적에 부응하게 소득보충이 아닌 사회참여를 통하여 건강증진과 건강한 노후생활을 통하여 노후 삶에 대한 질 향상이 되었는지를 연구했다. 이 연구를 통하여 노인 일자리사업(사회활동 지원사업)이 지속적으로 노인의 소득보충과 더불어 사회참여의 기회를 더욱 넓힐 수 있는 계기를 마련하고자 했다. The average life expectancy in Korea was 62.3 in 1970 and 82.3 in 2016, which increased by more than 20 years and it’s expected to enter super-aged society after passing through the aged society. The poverty, loneliness, inactivity, and disease which were called as four problems of senior citizens have become social issues. Thus, the project for creating job opportunities for the elderly(Project supporting social activity of seniors) was carried out as the senior welfare policy. First purpose was to help the elderly engage in society actively, make their income and build up health while preventing the problems of the aged and reducing social cost as well. The second purpose was to help them live healthy later lives through various jobs. This study aimed to investigate whether the project for creating job opportunities for the elderly increased quality of later lives through health promotion and healthy later lives while engaging in the society, rather than making income according to its purpose, by conducting the survey before and after participating in the project for creating job opportunities for the elderly. It’s expected to see this study could contribute to making more opportunities for the senior citizens to engage in the society continuously, rater than making money.

Efficacy of Bladder-Preserving Therapy for Patients with T3b, T4a, and T4b Transitional Cell Carcinoma of the Bladder

천재우,정현철,송재만 대한비뇨의학회 2010 Investigative and Clinical Urology Vol.51 No.8

Purpose: Radical cystectomy has been the most widely used method in the treatment of bladder cancer, but it is limited by major problems. Therefore, we investigated the results of bladder-preserving treatment in patients with T3b, T4a, and T4b transitional carcinoma of the bladder who underwent transurethral resection of bladder cancer and subsequent administration of chemotherapy. Materials and Methods: Of all patients who were diagnosed with bladder cancer and underwent bladder-preserving treatment between January 2001 and August 2008, 78 patients with at least 12 months of follow-up data were enrolled in this study. All patients received gemcitabine (1,000 mg/m2) and cisplatin (70 mg/m2) once per month postoperatively for a total of 6 months and completed a follow-up visit every 3 months. The patient survival rate and prognostic factors (age, tumor size, differentiation, number of lesions, stage, and presence of hydronephrosis) were assessed. The Kaplan-Meier method was used to analyze survival rate, and Cox multiple regression analysis was used for prognostic factors. Results: The mean patient age was 68.32±8.6 years, the mean duration of follow-up was 54.70±32.8 months, and the median duration of follow-up was 49.0 months. The 5-year survival rate was 66.2%. Single lesions were found in 28 cases (35.9%), and multiple lesions were found in 50 cases (64.1%). Stage T3b lesions were identified in 56 cases (71.8%), stage T4a lesions were identified in 16 cases (20.5%), and stage T4b lesions were identified in 6 cases (7.7%). Tumor size was less than 4 cm in 4 cases (59.0%) and greater than 4 cm in 32 (41.0%). Hydronephrosis was present in 21 cases (26.9%). In the 5-year survival analysis, prognostic factors significantly influencing survival rate were T-stage of the tumor and absence of hydronephrosis and complete regression after treatment (p<0.05). Multivariate analysis revealed that tumor stage and the absence of hydronephrosis were statistically significant prognostic indicators. Conclusions: In patients with T3b, T4a, and T4b transitional carcinoma of the bladder, bladder preservation may prevent a decrease in quality of life. Also, our findings suggest that this approach could be considered a primary treatment option for patients with T3b stage tumors without evidence of hydronephrosis.

논 토양에서 분리한 Pseudomonas sp. JK-7에 의한 Acrylamide의 생분해에 영향을 미치는 물리화학적 요인

천재우,호은미,오계헌 한국미생물학회 2004 미생물학회지 Vol.40 No.1

논 토양에서 acrylamide의 분해능이 탁월한 세균 Pseudomonas JK-7를 분리하여 acrylamide의 생분해에 영향을 미치는 물리화학적 요인을 조사하였다. 초기 실험에서 acrylamide를 유일 탄소원과 질소원으로 하여 호기적 조건에서 생장할 수 있는 세균 JK-7을 논의 토양표본에서 분리하였다. BIOLOG system을 이용한 생리학적 분석으로 Pseudomonas속(genus)임을 확인하였고, 이 세균을 Pseudomonas sp. JK-7로 명명하였다. 분리 균주 JK-7은 50 mM acrylamide를 배양 72시간 이내에 완전히 분해하였다. 배양기간 중에 acrylamide 분해 중간대사 물질로서 acrylic acid가 나타나는 것을 HPLC를 통해 확인하였으며, 배양초기에 배양액에 존재하지 않았던 ammonia가 배양기간 중에 관찰되었다. 배양초기 pH는 7.0이었으나 acrylamide가 완전히 분해된 후 배양액의 최종 pH는 8.7이었다. Acrylamide 분해와 JK-7의 생장에 대한 pH의 영향을 조사한 결과, PH 5, pH 7 그리고 pH 9에서 생장과 분해가 이루어졌으나, pH3과 pH11에서는 거의 관찰되지 않았다. 부가 탄소원의 존재하에서 JK-7에 의한 acrylamide분해에서 glucose, fructose, citrate 또는 succinate를 각각 첨가하여 조사한 결과, 부가 탄소원이 없을 때보다 acrylamide분해와 JK-7의 생장은 가속화되었다. 또한 부가질소원 첨가에 따른 영향조사 실험에서, yeast extract는 acrylamide분해와 JK-7의 생장을 촉진시켰다. 그러나 다른 질소원인 $(NH_{4})_{2}SO_{4}$, $NH_4Cl$ 그리고urea는 생장과 분해에 본질적으로 큰 영향을 미치지 않았다. 금속이온에 대한 영향으로 배지내에 $ZnSO_{4}$를 첨가하였을 때 분해와 생장이 진행되었으나, $AgNO_{3}$, $CuSO_{4}$ 또는 $HgCl_{2}$를 첨가하였을 때 acrylamide분해와 JK-7의 생장이 이루어지지 않았다. The purpose of this work was to investigate the relationships between acrylamide degradation by Pseudomonas sp. JK-7 and several relevant physicochemical environment parameters. In initial experiments, the bacterial culture, strain JK-7 isolated from paddy soil sample was developed to grow aerobically with acrylamide as the sole source of carbon and nitrogen. The bacterium was identified as genus Pseudomonas in the basis of use BIOLOG test, and designated as Pseudomunas sp. JK-7. Strain JK-7 could degrade 50 mM acrylamide completely within 72 hours of incubation. Major intermediates resulting from acrylamide degradation were not detected with the HPLC methodology except acrylic acid which appeared to accumulate transiently in the growth medium. The pH increased from 7.0 to 8.7 with complete degradation of the initial 50 mM acrylamide within 72 hours of incubation. pH control in the range of 5 to 9 influenced the growth of JK-7 and acrylamide degradation, whereas it was not examined the growth and degradation at pH 3 or pH 11, respectively. The effect of supplemented carbons (e.g., glucose, fructose, citrate, succinate) on the acrylamide degradation by the test culture of JK-7 was evaluated. The results indicated that the addition of carbons accelerated the bacterial growth and acrylamide degradation compared to those in the absence of supplemented carbons. The effect of supplemented nitrogens on the degradation was monitored. Increasing concentrations of yeast extract resulted in higher growth yield, based on the turbidity measurement, and complete degradation of acrylamide. However, acrylamide degradation was essentially uninfluenced by the addition of $(NH_{4})_{2}SO_{4}$, $NH_4Cl$ or urea. Addition of $AgNO_3$, $CuSO_4$ or $HgCl_2$ except $ZnSO_4$ in the test culture inhibited the degradation of acrylamide and growth of JK-7.

부고환에 발생한 악성 중피종

천재우(Jae Woo Cheon),김대일(Dae Il Kim),변현근(Hyun Geun Byun),주정민(Jung Min Joo),정제흥(Jae Heung Jung),김성진(Sung Jin Kim),정현철(Hyun Chul Jung) 대한비뇨기종양학회 2010 대한비뇨기종양학회지 Vol.8 No.2

식품 및 환경, 기타 새우양식장에서 분리한 해양세균 Bacillus sp. CK-10과 Bacillus sp. CK-13에 의한 양식사료에 포함된 질소와 인의 동시제거

천재우 ( Jae Woo Chun ),마채우 ( Chae Woo Ma ),강형일 ( Hyung Yeel Kahng ),오계헌 ( Kye Heon Oh ) 한국미생물생명공학회 2005 한국미생물·생명공학회지 Vol.33 No.2

새우양식장에서 분리한 Bacillus sp. CK-10과 Bacillus sp. CK-13에 의한 질소와 인의 제거

천재우(Jae-Woo Chun),마채우(Chae-Woo Ma),이상현(Sang-Hyun Lee),오계헌(Kye-Heon Oh) 한국생물공학회 2005 KSBB Journal Vol.20 No.2