Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

STZ 유발 당뇨쥐에서 시간에 따른 생리적 항산화계의 변화

이순재,양정아,김성옥,최정화,신주영,채영미,차복경 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.5 No.-

The purpose of this study was to investigate change of physiological antioxidative system according to the time in streptozotocin-induced diabetic rats. Sprague-Dawley male rats weighing 150±10gm were randomly assigned to one normal and five STZ-induced diabetic groups. Diabetic groups were classified to experimental period. Diabetes was experimentally induced by intravenous injection of 55 mg/kg of body weight of STZ in citrate buffer(PH 4.3) after 6 weeks feeding of three experimental diets. Animals were sacrificed at 0, 3, 6, 12, 18th days of diabetic states. 1. Activity of superoxide dismutase(SOD) in liver was increased in that of diabetic mellitus(DM) groups at 3th day but that of DM groups was not significant from 6th day according to period. Glutathion peroxidase(GPX) was significantly decreased in DM groups from 6th day 2. Reduced glutathione(GSH) contents in liver significantly decreased in diabetic groups from 3rd day compared with those of DM groups according to the period. Oxidized glutathione(GSSG) was higher from 6th day. GSH/GSSG ratio was significantly lower than that of normal group from 3rd day to all experimental period 3. Contents of vitamin E in liver of DM groups were significantly decreased compared with that of normal group from 6th day. 4. Lipid peroxide(LPO) contents in liver of DM groups were significantly increased compared with that of normal group from 3rd day. The present results indicate that STZ-induced diabetic rats were reduced by antioxidative defense system and taken by peroxidate damage in tissue compared with normal group from 3rd day or 6rd day after injection STZ. It lended to acceleration all diabetic groups but no significance according to the experimental time.

기능성 소화불량증에서 증상에 따른 고형식의 반위배출시간의 차이

김영호,이풍렬,성인경,손희정,김재준,고광철,백승운,이종철,최규완 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.3

경인지역 도금사업장 근로자의 산업보건관리제도에 대한 인식

이경용,이관형 大韓産業醫學會 1992 대한직업환경의학회지 Vol.4 No.1

도금작업장의 근로자들을 대상으로 도금작업장의 산업보건관리제도에 대한 인식을 알아보기 위하여 유해인자에 대한 인식여부 그리고 방진마스크 등의 보호구와 작업장 환경개선 시설의 하나인 환기시설 등에 대한 유용성을 알아보았으며, 다음으로 도금작업장의 산업보건관리 제도인 작업환경 측정과 근로자 건강진단 등에 대한 주관적인 평가정도를 알아보았다. 보호구의 지급현황과 착용여부를 확인한 결과 보호구는 조사대상의 약 90%의 근로자들이 보호구를 지급받고 있었으나 지급받은 보호구를 항상 착용하는 근로자는 지급받은 근로자들중 20%에도 미치지 못하는 것으로 나타났다. 보호구를 착용하지 않는 이유 중에서 가장 많은 응답비율을 보인 것인 답답해서였다. 방진마스크가 답답하여 항상 착용하지 않고 있음에도 보호구의 유용성에 대해서는 절반이상의 근로자들이 유용성을 인식하고 있었다. 다음으로 환기시설의 유용성 대해서는 조사대상 근로자들중 80%이상의 근로자들이 유용하다는 응답을 보였다. 작업장 환경에 대한 인식과 태도의 경우 유해인자의 발생을 인식하고 있는 근로자가 절반에도 미치지 못하는 것으로 나타났으며, 작업환경측정으로 작업장의 유해인자를 얼마나 평가할 수 있다고 생각하는지에 대해서는 약 60%의 근로자들이 어느정도 평가할 수 있다고 응답하였다. 도금작업이 인체에 미치는 영향에 관한 질문에서는 거의 대부분의 근로자들이 도금작업이 인체에 미치는 영향을 알고 있다고 응답하였으나, 비중격천공을 들어본 적이 있는지에 대한 질문에서는 약 60%의 근로자만이 비중격천공이라는 직업병을 들어보았다고 응답하고 있었다. 또한 근로자 건강진단을 통해 직업병을 발견할 수 있다고 응답한 근로자는 전체의 57%였다. 이상의 단일변량 분석결과를 바탕으로 방진마스크의 유용성과 환기시설의 유용성에 대한 인식, 유해인자 평가를 위한 작업환경 측정 및 직업병 조기발견을 위한 근로자 건강진단에 대한 주관적 평가에 영향을 미치는 인자가 무엇인지를 알아본 결과 방진마스크의 유용성과 작업환경 측정에 대한 주관적 평가정도는 조사된 어떠한 요인도 관련성이 없는 것으로 나타났다. 다음으로 환기시설의 유용성에 대한 인식에서는 연령, 도금작업의 종류, 월평균임금수준 등이 영향을 미치는 것으로 나타났다. 근로자 건강진단에 대한 주관적 평가는 연령과 현재 도금작업장의 근무기간 등에 따라 차이가 있는 것으로 나타났다. The purpose of this study is to investigate the present stage of workers' recognition about the institutions of occupational health management in his workplace. In general, workers' recognition about the institutions of occupational health management is related with workers' health behavior for preventing occupational diseases and improving their health status. The subjects of this stady are those who work in the plating industry. The results of the study are as follows : 1. More than half of workers recognize the usefulness of the protective device, and the ventilation device in the workplace. 2. Only 20% of workers have always taken the preventive device. 3. About 60% of workers are aware of occupational diseases in the plation industry. 4. The recognition of the usefulness of the ventilation device the work place is related with age, kinds of plating working part, and the level of monthly wages. 5. The subjective assessment about as mass screenign test regular health examination is related with the age and therwork duration.

總輸膽管 結紮術후 Hepatic Glycogen 變化에 관한 形態學的 硏究

李亨周,李永浩,朴景蘭,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

For the study on the changes of hepatic glycogen after common bile duct ligation, Sprague -Dawley rats (b.w. 250± 20gm, male) were used as experimental animal. In expermental group, at 3hrs, 6hrs, 9hrs, 12hrs, 24hrs, 48hrs, 72hrs, 96hrs, and 120hrs after ligation, the common bile duct at the point of about 1cm proximal portion from its duodenal opening, the liver specimens were collected under ether anesthesia. Simple laparotomy was performed in control rats. After fixation, the specimens were sectioned 5㎛ thickness and stained with Periodic Acid Schiff, and observed under light microscope. Also, transmission electron microscopic photos were obtained with H-7000(Hitachi Co.) TEM. The results were as followings: 1. PAS reactions were changed into negative 6hours after common bile duct ligation. 2. Glycogen depletion after common bile duct ligation was closely related with functional lobule, but not in simple laparotomized rats. 3. In the common bile duct ligated rats, PAS negativity continued in the zone I until 120hours after ligation.

모기업 협력업체 산업안전보건관리에 대한 인식

이경용,이관형,오지영,서남규,손두익,갈원모,신문진 한국안전학회 2003 한국안전학회지 Vol.18 No.4

This study is planned to investigate the attitude toward the safety and health management of contractor comparry. Under the contract based production system, all of activities including safety and health management in the contractor company are depended upon the contract. How to make contract influence the worker's health of contractor company. Work's health of contractor company can be protected by efforts of company of contract-out and contractor company, especially their safety managers. The modelling of the effective safety and health management system for contractor company should consider the need of safety manager of each company and employer of contractor company. Data is collected from safety managers of 3 contract out companies as electronic and electrical manufacturing industry and 55 safety managers, 57 employers of their contractor companies using self administered survey with structured questionnaire. The most of all respondents want to support from the contract out companies. The most important items supported from contract out company is the information based on the safety information network between each company. Safety manager and employer of contactor company also itemized safety education and training in the supporting system from contract out company. These results can be generalized to survey on the other industries.

Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석

이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-

A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.

제조업 근로자의 작업환경인지도와 스트레스와의 관련성 연구

오영아,이명선,이경용 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.3

목적 : 한국산업안전공단의 '1999년 근로자건강실태조사'를 분석의 기초자료로 하였으며, 연구대상인 전국의 근로자 6,764명을 대상으로, 신체적 스트레스, 정신적 스트레스와 일반적 특성, 작업조건과의 관련성을 분석하였다. 방법 : 표본으로 추출된 근로자들에게 구조화된 설문지를 통하여 1:1 면접조사를 시행하였으며, 자료는 SPSS 10.0을 이용하여 통계 처리하였다. 결과 : 1. 일반적 특성에 따른 스트레스 수준은 여성이 남성보다 높은 수준을 보여주었으며(p<0.001), 연령이 낮을수록(p<0.001), 규모는 클수록 그 수준이 높았다(p<0.01). 2. 근로조건에 따른 스트레스 수준은 작업시간이 많을수록(p<0.001), 휴식시간이 없을 때(p<0.05) 그 수준이 증가하였고, 작업공간이 좁고 작업밀도가 강할 때 스트레스가 높았다(p<0.001). 물리적 작업 환경에서는 습도와 작업장안전에서 문제가 있다고 느낄 때 스트레스가 높았으며(p<0.01), 소음이 문제가 있고(p<0.01), 분진 및 환기와 휴식공간과 식당, 채광조명에 문제가 있을 때 스트레스 수준이 높았다(p<0.05), 사회적 작업환경에서는 직무요구도와 직무통제성이 높고, 사회적지지가 낮을 때 스트레스 수준이 증가하였고, 이는 통계학적으로 유의하였다(p<0.001). 3. 다단계 중회귀분석을 통한 신체적 스트레스에 영향을 미치는 요인을 분석한 결과 작업장 안전이 5.1%로 가장 높은 설명력을 보여주었고, 다음으로 직무요구도, 연령, 작업밀도, 습도, 성별, 작업시간, 규모, 공식적인 휴식시간 유무의 순으로 선정되었는데 이들 요인에 의한 전체 설명력은 12 %였다. 또한 정신적 스트레스에 영향을 밀치는 요인을 분석한 결과 직무 요구도가 4.8 %로 가장 높은 설명력을 보여주었고 다음으로 습도, 연령, 성별, 직무 통제성, 작업장 안전, 사회적지지, 작업공간, 규모, 채광조건, 공식적인 휴식시간 유무, 작업시간의 순으로 선정되었는데 이들 요인에 의한 전체 설명력은 13.3 %였다. 결론 : 근로자의 작업환경이 스트레스와 관련성이 높은 것을 알 수 있다. 근무조건, 물리적 작업환경, 사회적 작업환경을 꾸준히 관리하고 개선할 때, 근로자의 스트레스가 감소할 것이고, 작업능률을 극대화 할 수 있을 것이다. Objectives : In this study we performed an analysis of the stress associated with the working conditions of 6,764 workers based on 'A survey of healthu condition of workers on 1999' by the Korean Occupational Safety & health Agency. The Purpose of this study was to provide basic data for health education and management for industrial workers' health promotion. Methods : Collected data were analyzed using the t-test. ANOVA and mutiple regression analysis, using the SPSS 10.0 program. Results : When the degrees of stress were analyzed according to demographic characteristics, females were found to have higher levels of stress than males (p<0.001). Furthermore, as age was lowered and as the work apsce became larger, the degrees of a stress were also found to be higher (p<0.001 and p<0.01, respectively). When the degrees of stress were analyzed according to working conditions, longer working hours (p<0.001) and reduced break times (p<0.05), gave rise to higher degrees of stress. Also, as the workspace was reduced in size and as the work intensity was augmented, the degrees of a stress were made higher (p<0.01). In terms of physical working conditions, humidity and a lack of sense of security resulted in higher degrees of stress (p<0.01). When the wokers sense that their environment is noisy or dusty has poor lighting or ventilation or that the rest room and cafeteria facilities are inade-quate, the degrees of stress are found to be higher. As regards the social environment, when the workers sensed that the degree of job demand or job control was higher or that the degree of social support was lower, the degrees of stress were increased (p<0.001). Among those factors which influenced physical stress in the stepwise multiple regression analysis, the primary factor was found to be the safety of the working place (5.1 %). Other factors which influenced physical stress were degree of job demand, age, work intensity, humidity, sexual discrimination, length of working hours, size of work space and the existence or not of a regular break time in this order. Total R^2 due to these factors was 12%. Among those factors which influenced mental stress in the stepwise multiple regression analysis, the primary factor was the degree of job demand (4.8 %). Others factors which infuenced mental stress were humidity, age, sexual deicrimination, the degree of a job control, safety of the working place, degree of social support, a working site, size of the work space, quality of lighting, the existence or not of a regular break time and length of working hours in this order. Total R^2 due to these factors was 13.1 %. Conclusions : The above results shows that working conditions are strongly related to stress. Furthermore, when both physical and social working conditions are contimuously managed and improved, workers may not only reduce their levels of stress but also maximize their working efficiency.

호흡기계 감염에 대한 Broncho-vaxom^�의 임상적 효과

최호경,이건수,서종진,정용헌 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

Thirty children with respiratory tract infections, 17 with acute and chronic bronchitis, 5 with sinusitis, 4 with rhinitis, 3 with asthma, 1 with tonsillitis, were given Broncho-vaxom^?? one capsule per day on an empty stomach for 1 month to evaluate the efficancy of the drug on acute and chronic respnatory tract infections in children. After the medication, we observed the changes of clinical symptoms, and side effects. The results are as follows; 1. The symptomatic improvements after Broncho-vaxom^?? administration were as follows; expectoration(80%), fever(72%), rhinorrhea(67%), hoarseness(67%), and coughing(65%). 2. Combined treatment with antibiotics was necessary in 19 cases(63%). 3. The degree of response were observed as mild improvement in 3 cases(10%), moderate improvement in 13 cases(84%), and excellent improvement in 1 cases(47%). 4. The side effect of Boncho-vaxom was not observed during this study.

소규모사업장 보건관리기술지원사업 지원여부에 따른 사업장내 의사소통 비교

정최경희,박혜숙,김정연,이경용,현성민,오지영,김수근,김현주,하은희 이화여자대학교 의과대학 2003 EMJ (Ewha medical journal) Vol.26 No.2

목적: 소규모사업장 보건관리기술지원사업 지원여부에 따라 사업장내 위험의사소통채널이 마련되었는지와 이 채널이 기능을 하는지, 그리고 의사소통채널마련과 사업장내 위험의사소통 활성화의 기본적이고 중요한 배경이 되는 사업주의 산업보건에 대한 관심이 차이가 있는지에 대해 살펴보고자 하였다. 방법: 서울, 안산, 대전, 광주, 포항 지역에서 보건관리기술지원사업을 받은 사업장과 받지 않은 사업장을 대상으로 비례층화무작위표본추출법을 사용하여 추출한 후 1991년 12월 6일부터 10일간 우편설문조사를 실시하였다. 설문대상은 각 사업장의 남녀 각 1명, 보건업무담당자, 사업주였으며, 설문내용은 사업장 및 응답자의 일반적 특성 및 사업장내 위험의사소통이었다. 설문에 응답이 온 463개 사업장의 자료를 자료처리하여 총 228개 사업장(단해년도지원 사업장 189개, 연속2년지원 사업장 13개, 비지원사업장 26개)을 대상으로 분석하였다. 결과: 사업장내 의사소통에 대한 단변량분석에서는 보건업무 담당자의 선정여부만이 통계적으로 유의한 차이가 있었다. 소규모사업장 보건관리기술지원사업을 지원받지 않은 사업장과 지원받은 사업장의 사업장내 위험의사소통을 비교하기 위한 로지스틱회귀분석 결과, 모든 항목에서 통계적으로는 유의한 차이를 보여주지 않았다. 그러나 사업주의 관심(OR ; 1.63, 95% CI ; 0.38~7.01)과 보건업무담당자의 선정(OR ; 3.67, 95% CI ; 1.00~13.44) 및 결정권한(OR ; 1.48, 95% CI ; 0.25~8.91), 사업주의 산업보건문제 소통(OR ; 3.07, 95% CI ; 0.81~11.71), 보건업무담당자의 정보요청경험(OR; 4.48, 95% CI ; 0.97~20.57)에서는 지원받지 않은 사업장에 비해 지원받은 사업장에서 교차비가 증가하는 양상을 보여주었다. 반면 노동자의 산업보건문제 소통가능성여부는 오히려 교차비가 0.53(95% CI ; 0.11~2.44)로 감소하는 경향을 보여주었다. 결론: 소규모사업장 보건관리기술지원사업을 의사소통의 측면에서 평가해보면, 사업주나 보건업무담당자에게는 다소 효과가 있었지만 노동자가 지속적으로 참여할 수 있도록 의사소통문화를 바꾸어내는 데에는 한계가 있었다. 개선을 위해서는 노동자 중심의 접근 프로그램의 개발 및 시행이 시급하다 할 수 있다. Objectives :This study was to evaluate the effectiveness of the government-funded subsidized occupational health program for small-scale enterprises(GSOHP_SSE) by occupational health com-munication in workplace. Methods : We sampled the 1,835 enterprises of study by proportional stratified random sampling among industries of supported and not supported by GSOHP_SSE in Seoul, Ansan, Daejeon, Kwang-joo and Pohang. Information on the general characteristics of enterprises and respondants and occupational health communication in the workplace was collected on self-reported questionnaires by post-survey between December 6 and 15 in 2001. We received answers from 463 enterprises and analyzed 228 enterprises through data-cleaning by logistic regression to evaluate effectiveness of GSOHP-SSE about occupational health communication in the workplace. Results : By multiple logistic regression analysis, GSOHP_SSE turned out not to be statistically significant factor in all dependent variables about occupational health communication in the work-place. The concern of owner(odds ratio (below OR) ; 1.63, 95% confidence interval(below 95% CI) ; 0.38-7.01), formation(OR ; 3.67, 95% CI ; 1.00-13.44) and empowerment(OR ; 1.48, 95% CI ; 0.25-8.91) of health manager, request about occupational health problem of health manager(OR ;4.48, 95% CI ; 0.97-20.57) and occupational health communication of owner (OR ; 3.07, 95% CI ; 0.81-11.71) had the trend increasing OR in the industries supported GS-OHP_SSE relative to the industries not supported. But in recognition of possibility on occupational health communication of laborers, OR of GSOHP_SSE was decreased in 0.53(95% CI ; 0.11-2.44). Conclusions: This study found that the GSOHP_SSE was some effective to the side of employer and health manager, but had a limitation about change of communication culture useful to the laborers. We suggested the laborer-centered health training programs or the empowerment-based health training programs for effective occupational health management in the workplace.