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PCR(Polymerase chain reaction) is a technique to replicate and amplify a desired part of DNA. It is used in various aspects such as DNA fingerprint analysis and rare DNA amplification of an extinct animal. Especially in the medical diagnosis field, it provides various measurement methods at the molecular level such as genetic diagnosis, and is a basic tool for molecular diagnostics. The internal shape of the plastic vial tube for PCR analysis used in the DNA detection process, and the surface roughness and internal cleanliness can affect detection and discrimination results. The plastic vial tube demanded by the developer of the PCR analysis equipment should be changed to a structure that eliminates the residual washing solution in the washing process to ensure the internal cleanliness. Thus the internal structure and the internal surface design for improving the PCR amplification efficiency are key issues to develop the plastic vial tube for the DNA detection process.
Hepatic hemangioma is the most common benign tumor of the liver which presents typical angiographic features, and can be usually differentiated from other tumorous conditions by various radiographic methods including angiography. However a review of the literatures shows a few cases with normal angiogram. The causes of nonopacification on angiogram described in the literatures are small size, fibrosis of the tumor, and dilution phenomenon of injected dye. We report a case of hepatic hemangioma, which showed normal angiogram, because of blood supply from a feeding vessel branched from portal vein.
The fifty gastrointestinal polyps, which were removed endoscopically frorn 47 patients at the Seoul National Univeraity. Hospital from January, 1978 to September, 1983, were reviewed with regard to the clinical and pathological features. The observed data are as follows; 1) Fifty polyps were reoved from 10 patients with gastric polyps and 37 patients with colonic polyps. Out of 37 patients 26 were male and 21 female, the sex ratio being 1.2: 1. 2) The gastric polyps were located in the body and antrum and 90 per cent of the colonic polyps were found in the left colon, especially in the rectosigmoid colon. 3) Out of the 10 gastric ployps snared, 8 could be retrivevd, 2 being lost. The histological examination showed that 3 were hyperplastic polyps, 2 gastritis verrucosa, 1 inflammatory polyp and the remaining 1 revealed intramucosel carcinoma with borderline epithelium. 4) Fourty polypectomies were performed in 37 patients with colonic polyps. Histological examination revealed that 24(60%) were adenomatous polyps, 9(22.5%) retention polyp, 4 (10.0%) inflammatory polyp and 3(7. 5%) Peutz-Jegher's poIyps. Among the adenomatous polys, 4 cases were complicated with focal adenocarcinoma. 5) Except the 2 cases of post-polypectomy bleeding, no other complication was encountered. The one case of hemorrhage after gastric polypectomy was treated with transfusion of 2 pints of whole blood, and the other case after colonic polypectomy required exploratory laparotomy because of inability of bleeding control by electrocoagulation.
The purpose of this study was to investigate the distribution of the physique, muscular strength, antioxidative stress for middle intensity resistance(weight training) training. The subjects of this study were consisted with 10 adult males. The weight training program of 8 stations performed on 3 circuits, 3day/week. The subjects of exercise intensity exercised at 55~60% of 1-RM, executing as many repetition as possible in 30 sec, followed by 15sec rest as the subject moved to the rest station the training program. The result were as follow; 1. Muscle strength increased was significantly in post middle intensity weight training. 2. MDA, catalase and vitamin-E decreased was significantly in post middle intensity weight training. 3. Phospholipid and CK decreased was significantly in post middle intensity weight training. It was concluded that weight training of 55~60%HRmax intensity is a good general conditioning activity, that more exercise participator hoped to take part in this paper method.
Background/Aims: We aimed to elucidate the prevalence and risk factors of HGV infection in patients with chronic renal failure undergoing hemodialysis, and to define the potential etiologic-role of HGV in the development of chronic liver disease. Methods: One-hundred and seventy-eigh patients with chronic renal failure undergoing hemodialysis were included. After extraction of RNA from serum, reverse transcription polymerase chain reaction was performed using the primers from the NS5a region. The PCR products were analyzed by liquid hybridization. Results: The prevalence was 9.0% (16/178). The prevalence rates were not statistically different according to the presence (8.5%) or abscence (10.8%) of past history of blood transfusion, patient's age or maintenance duration of hemodialysis (p$gt;0.05). Anti-HBc-positive patients (12.4%, 16/129) showed significantly higher prevalence rate (p=0.01) of HGV infection than anti-HBc-negative patients (0%, 0/49). Twelve of the 14 HGV positive patients without co-infection showed persistently normal ALT level during a year of follow-up. Conclusions: The prevalence of HGV in patients with chronic renal failure undergoing hemodialysis is significantly higher than that of volunteer blood donors. HGV may share the route of transmission with adulthood HBV infection. The etiologic-role of HGV in the development of chronic liver disease may be considerably poor. (Kor J Gastroenterol 1999;33:78 - 89)
The Purpose of this paper is to evaluate the clinical efficacy of the titrated extract of Centella asiatica (TECA: Madecassol) in patients with peptic ulcer. Nineteen patients with gastric ulcer and thirteen patients with duodenal ulcer were given 60mg a day, P.O. and 20mg, I.M., 3 times a week of TECA and were followed up by gastroscopy at 4, 6, 8, and 10 weeks of treatment. The observed results are as followed: 1) Six (31.6%) out of 19 cases of gastric ulcers were completely healed off by 4 weeks, 14 (73.7%) by 6 weeks, and 17 (89.5%) by 8 weeks of treatment. One case was not healted up to 10 weeks of treatment. 2) Five (38.5%) out of 13 cases of duodenal ulcers were completely healed off by 4 weeks, 9 (69.2%) by 6 weeks and 11 (84.6%) by 8 weeks of treatment. One case was resistant to the treatment up to 10 weeks of observation. 3) There was no untoward side reaction, either subjective or objective, to the administration of the drug.