세포질내 정자주입법(ICSI)에 있어서 정자흡입 및 난자내 주입방법에 관한 연구

이택후,김항진,송건호,김대근,전상식,박윤규,서태광,전병균,류은경,이은숙,문진수,김광철 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Study on Method of Sperm Aspiration and Injection into an Oocyte in Intracytoplasmic Sperm Injection(ICSI) Immobilization of spermatozoa prior to intracytoplasmic sperm iniection(ICSI) sometimes results in crooked tail and this makes it difficult to aspirate sperm into an injection pipette tail first. Head-first sperm aspiration into an injection pipette avoid this problem due to the bigger size of the sperm head. The effect of head or tail-first sperm injection into an oocyte on fertilization cleavage, percentage of grade I embryos and development to blastocyst stage in ICSI program has been studied. A single living immobilized spermatozoa from oligoasthenozoospermic patient was injected into an oocyte head-first or tail-first according to the treatment. Eighteen hours after microinjection, oocytes ware inspected for survival and fertilization Fertilized oocytes with two pronuclei were cultured in 30μl drop of mHTF supplemented with 10% heat-inactivated follicular fluid(FF) at 37℃. On day 2. embryo transfer was performed with cleaved embryos. The remaining 2-8 cell stage embryos were co-cultured with BRL cells in mHTF + 10% FF for 72 hours and the developmental stage was observed. The data were analyzed by Analysis of Variance. A total of 164 oocytes from 36 cycles were assigned to earth treatment and ICSI was performed(88 head-first, tail-first). The rates of normal fertilization were 81.8% and 76.3% for head-first and tail-first, respectively. Of the fertilized oocytes, the percentage of cleaved embryos and the percentage of grade 1 embryo among cleaved embryos were 88.9% and 68.8%, 93.1% and 74.1% for head-first and tail-first, respectively. Of the 2-8 cell embryos cultured, 44.4%(16/36) and 50.0%(10/20) for head first and tail first, respectively developed to blastocyst stage. There were no differences in fertilization, cleavage, rates of grade 1 embryos, and development to blastocyst stage. In conclusion, head-first or tail-first sperm injection into an oocyte in ICSI program does not affect fertilization and subsequent embryo development to blastocyst stage in vitro.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Monitoring Plant Health using a Plant Microbial Fuel Cell

Tae Hang YOON,Hak Jin SONG,Wu-Young JEONG,Ji Eun KIM,Yunjeong YANG,Eun Bin LEE,Kwang Jin KIM,Ho Hyun KIM,Hyung Joo KIM 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

심부전에 대한 인공신장기의 임상적 고찰

윤기윤 ( Kee Yoon Yoon ),안명훈 ( Myung Hooo Ahn ),문병욱 ( Byung Ook Moon ),황위현 ( Wee Hyun Whang ),이태흥 ( Tae Hung Lee ) 대한내과학회 1967 대한내과학회지 Vol.10 No.2

대장내시경 전처치: 경구 Sodium Phosphate와 Polyethylene Glycol 용액의 비교분석

조항준,강윤식,김태수,정승용,김도선,이두한 대한대장항문학회 1997 Annals of Coloproctolgy Vol.13 No.2

Novel Techniques for Real Time Computing Critical Clearing Time SIME-B and CCS-B

Hung Nguyen Dinh,Minh Y Nguyen,Yong Tae Yoon 대한전기학회 2013 Journal of Electrical Engineering & Technology Vol.8 No.2

Real time transient stability assessment mainly depends on real-time prediction. Unfortunately, conventional techniques based on offline analysis are too slow and unreliable in complex power systems. Hence, fast and reliable stability prediction methods and simple stability criterions must be developed for real time purposes. In this paper, two new methods for real time determining critical clearing time based on clustering identification are proposed. This article is covering three main sections: (i) clustering generators and recognizing critical group; (ii) replacing the multi-machine system by a two-machine dynamic equivalent and eventually, to a one-machineinfinite-bus system; (iii) presenting a new method to predict post-fault trajectory and two simple algorithms for calculating critical clearing time, respectively established upon two different transient stability criterions. The performance is expected to figure out critical clearing time within 100ms-150ms and with an acceptable accuracy.

주사전자현미경을 이용한 피부사상균의 형태학적 관찰

윤경한,정태석,신항계,방동식,이광훈 대한의진균학회 1997 대한의진균학회지 Vol.2 No.2

Background: It is important to understand the morphologic characteristics for the identification of a microorganism. Electron microscopy has contributed a great deal to the field of mycology. Scanning electron microscope(SEM) has become a useful tool to study three-dimensional images of fungi. Vacuum dessicator aqueous fixation is useful sample preparation technique for SEM examination of fungal specimens without surface distortions. Objective : This study was done to observe the three-dimensional structures of dermatophytes preserving their natural growth characteristics. Methods : Trichophyton rubrum, T. mentagrophytes, Microsporum canis, M. gypseum, and Epidermophyton floccosum were isolated and cultured on Sabouraud agar, corn meal agar, or rice agar plate. Vacuum dessicator aqueous fixation was utilized as a preparation technique for SEM examinations of the fungi. Results : Vacuum dessicator aqueous fixation led us to examine the aerial mycelia including conidia of the dermatophytes under SEM. The five species of dermatophytes showed their own characteristic structures especially on microconidia and macroconidia. Conclusion : The use of ultrastructural research has made it possible to identify the species of fungi and can be expaned and improved along with the development of SEM and specimen preparation techniques.

Caudal Anesthesia하의 치핵절제술시 하부 직장점막하에 주사한 Lidocaine의 효과

김태수,김도선,강윤식,정승용,조항준,이두한 대한대장항문학회 1998 Annals of Coloproctolgy Vol.14 No.1

Sb-Al<i> _x </i>C<i> _y </i>-C Nanocomposite Alloy Anodes for Lithium-Ion Batteries

Hung, Nguyen Thanh,Park, Sung-Hoon,Bae, Joonwon,Yoon, Young Soo,Kim, Ji Hyeon,Son, Hyung Bin,Lee, Daeho,Kim, Il Tae,Hur, Jaehyun Pergamon Press 2016 Electrochimica Acta Vol. No.

<P><B>Abstract</B></P> <P>We present a facile approach to making Al-Sb, carbon-modified (Al-Sb-C) nanocomposites for use as new anode materials in lithium-ion batteries (LIB). Alloying is achieved by one step synthesis using high energy mechanical milling (HEMM), producing nanometer-sized alloy particles of Sb-Al<I> <SUB>x</SUB> </I>C<I> <SUB>y</SUB> </I>-C. Based on electrochemical analyses, we determined that Sb acts as an active material, and both Al and carbon create a hybrid buffering matrix that mitigates the volume expansion of the active material during lithiation/delithiation to a greater degree than that by a pure metallic matrix (AlSb). In addition, we optimized the stoichiometric ratio of Al and Sb with regard to specific capacity and cycling performance. Of the ratios tested, a 1:1 molar ratio of Al and Sb exhibited the best cycling performance (302.5mAhg<SUP>−1</SUP> after 200 charge/discharge cycles). Although our Al-Sb-C composite had low initial coulombic efficiency (∼59%), recovery to∼97% occurred within three cycles, indicating that initial side reactions are quickly reduced over the course of cycling. AlSb-C anodes also showed good rate capability and volumetric capacity. Overall, the new AlSb-C composite is a promising candidate for use as negative electrodes in lithium-ion batteries, providing an alternative to commercially available graphite electrodes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We fabricated Sb-Al<SUB>x</SUB>C<SUB>y</SUB>-C nanocomposite alloy anodes by high-energy mechanical milling for use in lithium-ion batteries. </LI> <LI> The electrochemical performances, morphologies, and crystallographic features of AlSb and AlSbC with three different mixing ratios of Al and Sb were investigated. </LI> <LI> We found that AlSb(1:1)-C showed the best electrochemical performances in terms of specific capacity, cyclic performances, and rate capabilities. </LI> <LI> The mechanism of formation of AlSbC was examined by using XRD, TEM, and DCP data. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>We fabricated the high performances of Sb-Al<SUB>x</SUB>C<SUB>y</SUB>-C nanocomposite alloy anodes using high-energy mechanical milling for use in lithium-ion batteries.</P> <P>[DISPLAY OMISSION]</P>

1,683예의 대장내시경 검사에서의 대장용종에 대한 전향적 분석

김태수,김도선,정승용,이두한,강윤식,조항준 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.6

Background/Aims: The colorectal polyp, particularly the adenoma, has been regarded as a precursor of cancer. The incidence of colorectal polyps has been reported at various rates according to investigation centers in foreign countries. In Korea, the incidence of colorectal polyps has been reported as very low according to the few reports, which was involved a few cases and were partial. Therefore, the aim of this study is to evaluate prospectively the incidence and the clinicopathologic features of colorectal polyps. Methods: A colonoscope was inserted up into the cecum in 1,889 patients among 2,001 trials from Oct. 1996 to Aug. 1997 (success rate: 94.4%). Of the 1,889 full colonoscopies, the following were excluded; 1) referred patients with suspicious colorectal cancer or polyps, 2) patients with suspicious rectal cancer determined by rectal examination, 3) patients who had follow-up colonoscopy after a polypectomy or cancer surgery, and 4) patients who had periodic colonoscopy due to FAP or HNPCC. There were a total of 1,683 full colonoscopies in this study. The majority of the total cases involved a colonoscopy due to benign anal disease, irritable bowel syndrome, or routinechecks for health. Results: There were 946 men (56.2%) and 737 women (43.8%). The mean age was 48.2 yrs (13∼88 yrs) for men and 48.1 yrs (18∼89 yrs) for women. 422 patients were found to have 645 colorectal polyps (1.52 polyps per patient). The incidence of polyps was 25.1% (32.0% for men, 16.1% for women) and increased after the 6th decade in men (44.0%) and in women (23.0%). 281 patients were found to have 426 colorectal adenomas. The incidence of adenomas was 16.6% (21.9% for men, 9.9% for women) and increased after the 6th decade, 32.0% in men, and 15.9% in women. Solitary polyps were present in 277 patients (65.6%) while 94 patients (22.3%) had two polyps and 51 patients (12.1%) had between 3 and 8 polyps. The polyp retrieval rate was 96.9%. There were 426 adenomas (66.0%), 75 hyperplastic (11.7%), 120 inflammatory (18.6%), and 24 mcellaneous (3.7%) polyps. Of the 426 adenoma, there were 397 tubular adenoma (93.2%), 18 tubulovillous adenoma (4.3%), 4 villous adenoma (0.9%), 6 in situ carcinoma (1.4%), and 1 invasive carcinoma (0.2%). 6 in situ carcinoma's were detected in tubular adenoma and 1 invasive carcinoma in tubulovillous adenoma. Of 645 polyps, the sessile type was 87.9%. The polyp size varied from 1 mm to 25 mm and 79.2% were under 5mm. Also, 85.7% of adenomas were sessile and 74.7% were under 5mm. 22.0% of polyps were located in rectum, 35.4% in sigmoid colon, 9.5% in descending colon, 13.3% in transverse colon, 19.8% in ascending colon and cecum. The distribution of adenoma was similar to that of polyp. 40.1% of adenoma showed moderate to severe dysplasia. Conclusions: This study revealed that the incidence of polyp in Korea was higher than that in previous reports and 42.6% of polyps were proximal to rectosigmoid colon. Therefore, we suggest that we should try to detect and remove colorectal polyps by more active colonoscopy.