Cellulomonas chitinilytica sp. nov., a chitinolytic bacterium isolated from cattle-farm compost

Yoon, M.-H.,Ten, L. N.,Im, W.-T.,Lee, S.-T. Microbiology Society 2008 International journal of systematic and evolutiona Vol.58 No.8

<P>A bacterial strain, designated X.bu-b T, with chitin-, xylan-, cellulose- and starch-degrading activities, was isolated from compost at a cattle farm near Daejeon, Republic of Korea. The strain comprised Gram-positive, aerobic or facultatively anaerobic, non-motile, rod-shaped bacteria. On the basis of an analysis of 16S rRNA gene sequences, the phylogenetic position of X.bu-b T was within the genus Cellulomonas, and the strain exhibited relatively high sequence similarities with respect to Cellulomonas biazotea DSM 20112T (98.1 %), C. cellasea DSM 20118T (98.1 %), C. fimi DSM 20113T (98.0 %), C. terrae DB5T (97.9 %), C. humilata ATCC 25174T (97.7 %), C. xylanilytica XIL11 T (97.5 %), C. uda DSM 20107T (97.4 %), C. gelida DSM 20111 T (97.3 %), C. iranensis OT (97.3 %) and C. flavigena DSM 20109T (97.0 %). The phylogenetic distance from other Cellulomonas species with validly published names was greater than 3 % (i.e. less than 97.0 % sequence similarity). Chemotaxonomic data also supported the classification of strain X.bu-b T within the genus Cellulomonas: L-ornithine was the cell-wall diamino acid, anteiso-C15:0 and anteiso-C17:0 were the major fatty acids, rhamnose, galactose, xylose and ribose were the cell-wall sugars, MK-9(H4) was the predominant menaquinone and diphosphatidylglycerol and phosphatidylglycerol were present in the polar lipids. The G+C content of the genomic DNA was 73.6 mol%. DNA-DNA hybridization experiments showed that the values for DNA-DNA relatedness between strain X.bu-b T and the phylogenetically closest neighbours were below 23 %. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain X.bu-b T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas chitinilytica sp. nov. is proposed. The type strain is X.bu-b T (=KCTC 19133T =DSM 17922T).</P>

Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea

Yoon, J.-H.,Kang, S.-J.,Lee, M.-H.,Oh, T.-K. Microbiology Society 2008 International journal of systematic and evolutiona Vol.58 No.8

<P>A Gram-negative, non-motile, rod-shaped, agarolytic bacterial strain, designated JW-26T, was isolated from seawater off Jeju Island in Korea, and was subjected to a polyphasic taxonomic investigation. Strain JW-26T grew optimally at pH 7.0-8.0, at 30 degrees C and in the presence of approximately 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain JW-26T joins Tamlana crocina HST1-43T, with which it exhibited a 16S rRNA gene sequence similarity value of 95.5 %. Strain JW-26T contained MK-6 as the predominant menaquinone and iso-C15:0, C15:0 and iso-C15:1 as the major fatty acids. The DNA G+C content was 36.8 mol%. There were no distinct phenotypic and, particularly, chemotaxonomic properties to differentiate strain JW-26T from the genus Tamlana. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain JW-26T is distinguishable from the type strain of T. crocina. On the basis of phenotypic, phylogenetic and genetic data, therefore, strain JW-26T represents a novel species within the genus Tamlana, for which the name Tamlana agarivorans sp. nov. is proposed. The type strain is JW-26T (=KCTC 22176T =CCUG 55327T).</P>

임신 및 각종 갑상선질환에서 갑상선 기능 판정에 관한 연구 : 혈청유리 T4의 진단적 의의에 관한 고찰 The diagnostic value of free thyroxine by RIA

이종철,유명희,윤휘중,신영태,정순일,조보연,이문호,이명철 대한핵의학회 1981 핵의학 분자영상 Vol.15 No.1

To evaluate the diagnostic accuracy of the measurement of free thyroxine(FT4) by radioimmunoassay, we measured free T4 and T4, T3, T3RU, TSH and TBG serum levels by radioimmunoassay in 18 healthy persons and 52 patients with various thyroid diseases and 11 normal pregnant women. The results are as follows. 1) In 19 cases of overt hyperthyroidism, T3, free T4 and FTI, T4/TBG ratio reflect hyperfunction in all cases. T4 is increased in 94%(18/19) and TBG and TSH are decreased in 79%(15/19). 2) In 8 patients with overt hypothyroidism, TSH is increased in all cases and free T4 and FTI is decreased in all cases. T4 is decreased in 87.5%(7/8), T3 is decreased in 75%(6/8) and T4/TBG ratio is decreased in 62.5%(5/8). 3) In 5 patients who are clinically in euthyroid state after treatment of hyperthyroidism, T4 free T4, FTI and TSH are in the normal range in all cases and T3 is normal in 60%(3/5) and slightly increased in 40%(2/5). 4) In 10 patients who showed clinically borderline hypothyroidism after treatment of hyperthyroidism, TSH is increased in all cases and free T4 and FTI are decreased in all cases, but T4 and T3, T4/TBG ratio are in the normal limit in all cases. So after treatment of hyperthyroidism, TSH, free T4 or FTI are recommended as optimal function test. 5) In normal pregnancy, free T4, FTI and T4/TBG ratio reflect normal function, but the other parameters unreliable due to the influence of increased TBG. Also TBG and TSH level in pregnancy is increased significantly compared with normal healthy control group. 6) The coefficients of correlation between T4 and FTI were 0.862(p〈0.001) and 0.685(p〈0.001) between free T4 and T4/TBG ratio. In most patients, diagnostic value of free T4 was comparable and even superior to FTI, so free T4 measurement can be used routinely with thyrotropin assay in the diagnosis of hypothyrodism or with T3 for the diagnosis of hyperthyroidism.

정상 임신에서 임신주기에 따른 갑상선기능의 변화

김원배,정재훈,윤보현,이석인,김민선,오태근,조보연,이홍규,고창순 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

It is well known that normal pregnancy is accompanied by a rise in serum concentrations of thyroxine-binding globulin(TBG) and human chorionic gonadotropin (hCG). Alterations of biochemical parameters of thyroid function are recognized during gestation and sensitive tests to evaluate the alterations easily are required. Therefore, a cross-sectional study was undertaken in 140 healthy pregnant women to evaluate the efficacy of free T_4 measured by 2-step RIA compared to other thyroid function tests and to confirm the changes of thyroid function according to the stages of normal pregnancy. The sensitivities of free T_4 index, free T_4(by 2-step RIA), T_3 and TSH were realtively high(99.3%, 93.6%, 92.9%, 83.6%, respectively) compared to those of T_4 and T_3 bead upgake(49.3%, 21.4%) during all stages of pregnancy. There were positive correlations between free T_4 index and free T_4 or total T_4(r=0.68, r=0.72; p$lt;0.001). The values of free T_4 index sharply decreased from 3.22+-0.10(meam +-SEM) during 6th-12th week to an plateau after 16th-20th week of gestation(p$lt;0.01). The serum concentrations free T_4 and T_3 bead uptake also significantly decreased from 1.65+-0.05 ng/dl, 24.7+- 0.7% during 6th-12th week to an plateau after 16th-20th week of gestation, respectively(p$lt;0.001), No differences were found in the changes of serum concentrations of T_3, T_4 and TSH according to the stages of pregnancy. In conclusion, it is adequate to measure some tests including free T_4 index and free T_4 to evaluate thyroid function during pregnancy. The thyroid physiology and changes of thyroid function according to the stages of pregnancy should be considered in the interpretation of thyroid function status during pregnancy(J Kor Soc Endocrinol 9: 183-189, 1994).

Distribution of T and B Lymphocytes in Peripheral Blood and Lymphoid Tissues of Bovine

Yoon, C. Y.,Kim, T. J.,Cho, J. G.,Chai, H. S.,Kim, J. M.,Song, H. J. 全北大學校 附設 畜産開發硏究所 1991 畜産開發硏究報告 Vol.4 No.-

소의 순환혈액 및 림프조직내의 T 및 B 림프구의 분포를 파악하고자 소흉선세포를 토끼에 과면역시켜 얻은 항혈청을 소의 적혈구, 간장분만 및 골수세포에 순차적으로 흡착시킨 항융선세포혈철(RABTS), 성숙 T 림프구에만 특이성을 가진 단클론항체 BLT_2, B림프구와 소수의 대식세포에 특이성을 가진 단클론항체 _6E_12 등을 1차항체로 이용하여 ABPC법으로 T 및 B 림프구를 동정한 바 다음과 같은 결과를 얻었다. RABTS 및 BLT_1으로 동정한 순환혈액내 단핵세포 (PB-MNC)중 T림프구의 비율은 각각 70.9±5.5% 및 59.0±8.7%이었으며, PB-MNC중 nylon wool 비부착세포를 BLT_1 및 _6E_12로 동정한 T 및 B 림프구의 비율은 각각 91.6±1.0%, 9.6±0.8%이였다. 또한 BLT_1 및 _6E_12로 동정한 서혜림프질, 장간막림프절, 비장 및 흉선의 T/B림프구의 비율은 각각 56.4±6.2/45.3±7.4%, 55.6±7.7/42.3±5.8%, 48.6±5.1/48.5±6.2% 및 23.0±4.8/5.6±2.1%이었으며, RABTS로 동정한 이들 림프조직내의 T림프구의 비율은 각각 76.4±3.4%, 74.2±8.2%, 73.9±5.5% 및 95.6±2.8%이였다. 이상의 결과로 미루어 PB-MNC내의 T림프구의 분리는 nylon wool column법이 보다 효과적임을 알 수 있었다.

남조류와 녹조류에서의 수소생산을 위한 배양 조건의 최적화

윤종현(J. H. Yoon),공경택(K. T. Kong),심상준(S. J. Sim),김미선(M.S. Kim),박태현(T. H. Park) 한국수소및신에너지학회 2002 한국수소 및 신에너지학회논문집 Vol.13 No.4

Blue-green algae and green algae have the unique characteristic of using CO₂ in the air as a carbon source and solar energy as an energy source and producing H₂ with water used as the primary electron donor. The cells take up CO₂ first to produce cellular substances, which are subsequently used for H₂ production. Since the optimal operating conditions for the CO₂ uptake and H₂ production are different, a two-stage system can be effectively employed to separate these two phases. For the efficient production of H₂ in the second stage, in this study, the culture condition of blue-green algae was optimized for the effective CO₂ uptake and cell growth in the first stage, and high cell density culture was carried out using repeated injections of CO₂. In green algae, the amount and patterns of organic compounds have a connection with growth stage or dark fermentation time. The hydrogen production was greatly influenced by dark fermentation time.

Effects of Lewis number and preferential diffusion on flame characteristics in 80%H₂/20%CO syngas counterflow diffusion flames diluted with He and Ar

Park, J.,Lee, D.H.,Yoon, S.H.,Vu, T.M.,Yun, J.H.,Keel, S.I. Pergamon Press ; Elsevier Science Ltd 2009 International journal of hydrogen energy Vol.34 No.3

Numerical study is conducted to grasp flame characteristics in H<SUB>2</SUB>/CO syngas counterflow diffusion flames diluted with He and Ar. An effective fuel Lewis number, applicable to premixed burning regime and even to moderately stretched diffusion flames, is suggested through the comparison among fuel Lewis number, effective Lewis number, and effective fuel Lewis number. Flame characteristics with and without the suppression of the diffusivities of H, H<SUB>2</SUB>, and He are compared in order to clarify the important role of preferential diffusion effects through them. It is found that the scarcity of H and He in reaction zone increases flame temperature whereas that of H<SUB>2</SUB> deteriorates flame temperature. Impact of preferential diffusion of H, H<SUB>2</SUB>, and He in flame characteristics is also addressed to reaction pathways for the purpose of displaying chemical effects.

Effects of insulin glulisine as mono- or add-on therapy in patients with type 2 diabetes mellitus

Kawamori, R.,Iwamoto, Y.,Kadowaki, T.,Iwasaki, M.,Kim, S.-W.,Woo, J.-T.,Baik, S.-H.,Yoon, K.-H. Blackwell Publishing Ltd 2009 DIABETES OBESITY AND METABOLISM Vol.11 No.9

<P>Aim</P><P>To evaluate the safety and efficacy of insulin glulisine (glulisine) with and without oral antidiabetic drugs (OAD; sulphonylurea or sulphonylurea + biguanide) relative to that of OAD alone in Japanese and Korean patients with inadequately controlled type 2 diabetes mellitus (T2DM).</P><P>Methods</P><P>In an open, randomized, parallel-group, comparative, controlled trial, 387 patients were randomized and treated with glulisine + OAD (n = 130), glulisine monotherapy (n = 127) or OAD only (n = 130) for 16 weeks. Glulisine was self-injected subcutaneously three times daily (0–15 minutes before meals) at a starting dose of ≥0.2 U/kg/day. Patients titrated the glulisine dose to achieve a 2-h postprandial plasma glucose (2h-PPG) level of 7.1–9.5 mmol/l (128–172 mg/dl) by administering at least one additional unit at each appropriate meal time if the 2h-PPG level was > 9.5 and < 11.1 mmol/l (> 172 and < 200 mg/dl) and by administering at least two additional units if the 2h-PPG level was ≥ 11.1 mmol/l (≥ 200 mg/dl). Therapy with OAD was continued at the stable baseline regimen. The primary efficacy endpoint was change in haemoglobin A<SUB>1c</SUB> (HbA<SUB>1c</SUB>) from baseline to endpoint in the intention-to-treat population.</P><P>Results</P><P>At baseline, therapy with OAD was a sulphonylurea only and a sulphonylurea + a biguanide in approximately 24 and 76% of patients respectively. Both glulisine groups had larger reductions in adjusted mean HbA<SUB>1c</SUB> than the OAD-only group (glulisine + OAD, −2.07%; glulisine monotherapy, −1.25%; OAD only, −0.61%). Superiority of glulisine + OAD and glulisine monotherapy vs. OAD only was shown by differences in adjusted mean HbA<SUB>1c</SUB> change from baseline values of −1.46% (p < 0.0001) and −0.64% (p < 0.0001) respectively. Both glulisine groups had better 2h-PPG control than the OAD-only group. Mean daily glulisine doses increased from baseline to endpoint (glulisine + OAD, 13.3–22.5 U; glulisine monotherapy, 14.2–38.0 U). The rate of all symptomatic hypoglycaemia events per patient-year in the entire treatment phase was 11.9 in the glulisine + OAD group, 8.8 in the glulisine monotherapy group and 1.7 in the OAD-only group. There was only one event of severe hypoglycaemia, which occurred in the glulisine + OAD group. Efficacy and safety were similar in Japanese and Korean subpopulations.</P><P>Conclusions</P><P>Both glulisine + OAD and glulisine monotherapy were well tolerated and effective for Japanese and Korean patients with T2DM mellitus inadequately controlled by OAD therapy alone.</P>

A Single-Flux-Quantum Shift Register based on High-$T_c$ Superconducting Step-edge Josephson Junctions

Sung G.Y.,Choi, C.H.,Suh J.D.,Han, S. K.,Kang, K.Y.,Hwang, J.S.,Yoon, S.G.,Jung, K.R.,Lee, Y.H.,Kang, J.H.,Kim, Y.H.,Hahn, T.S. The Korean Superconductivity Society 1999 Progress in superconductivity Vol.1 No.1

We have fabricated and tested a simple circuit of the rapid single-flux-quantum(RSFQ) four-stage shift register using a single layer high-$T_c$ superconducting (HTS) $YBa_2Cu_3O_{7-x}$ (YBCO) thin film structure with 9 step-edge Josephson junctions. The circuit includes two read superconducting quantum interference devices(SQUID) and four stages. To establish a robust HTS RSFQ device fabrication process, we have focussed on the reproducible process of sharp and straight step-edge formation as well as the ratio of film thickness to step height, t/h. The spread of step-edge junction parameters was measured from each 13 junctions with t/h=1/3, 1/2, and 2/3 at various temperatures. We have demonstrated the simplified operation of the shift register at 65 K.

공정육묘 온실의 표준모델 및 자동화 시스템 개발과 활용기술연구 4 : 제4장 공정묘 생산기술 개발:제1절~제2절 Chaper4 Development of Plug Seedling Production Technology

박중춘,민영봉,정병룡,설인준,이영만,윤용철,강호종,김태규,김광용,장사문,정한택,오태한,김평태,김진일,손영걸,박봉식,김시론,이형정,오상석,김명승,조정호,하종규,임동희,이은주,변정희,이정한,최우진,강환규 경상대학교 시설원예연구소 1997 施設園藝硏究 Vol.4 No.-