크롬(Ⅵ)의 체내 흡수와 소실속도에 관한 연구

김현영,이성배,임철홍,이권섭,정용현,이종성,한정희,전윤석,황호순,이용묵 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.1

The CrO3 mostly used in plating. metal surface disposal, leather, cosmetic manufacturing, as an experiment material by repeatedly inhaling and exposure the male S.D. rats at a 0.00, 0.2., 0.50, 1.25 mg/㎡ concentration(particle size: 0.5-0.5 aerosol)6hours a day, 5day a week in 13weeks comparing with 2weeks, 8weeks of recovery group about the noxiousness of the experiment animal and the reduce scale of the CrO3 in the internal organ especially in blook and respiratory organ with the period of convalescent and clearance. The experiment results which we received are as follows. 1. In blood the RBC, HGB and HCT experiment, rats with 0.20, 0.50 mg/㎡ concentration showed that there were some decreases but not dependent. The kidneys absolute weight compared with control group was reduced intentionally(p〈0.05) and the lungs absolute weight compared with control group showed intentional increase(p〉0.05). 2. After the exposure of the experiment material, the whole blook, l\blood plasma and red blood cell in blood by (x): the period of convalescent, per (y); the decreasing of Cr concentration, was y=66.51 e -0.057x, y=67.2 e-0.101x, y=70.01 e-0.030 in 0.50 mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life (day)was estimated 12.0, 6086, 23.0 each. 3, After the exposure of the experiment material, the experiment animals lung, liver and kidneys by(x); the period of convalescent, per (y); the decreasing of Cr conentration, was y=1808 e-0.00493x, y=12.02e-0.0297x, y=67.61 e-0.0292x in 0.50mg/㎡ exposure concentration by calculating the clearance coefficient of correlation, and the half life(day)was estimated 140.6, 23.3, 23.7, each, and including lung, liver with all of the experiment internal organs, the Cr clearance decreased as the exposure concentration increased.

SD Rats를 이용 1-hexene의 흡입독성 연구

김현영,임철홍,정용현,이권섭,이성배,이준연,한정희,전윤석,이용묵 한국환경독성학회 2001 환경독성보건학회지 Vol.16 No.4

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of I -hexene on Sprague- Dawley (SD) rats which were treated by inhalation. The results were as follows; I. The median lethal concentration (LC_(50)) was estimated 52,694 ppm (confidence limit 95%; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the l-Hexene were not observed with the acute inhalation dose. Gross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene, II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed I-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

S.D. Rats를 이용 1, 1-Dichloro-1-fluoroethane의 아민성 흡입독성 연구

김현영,이성배,임철홍,김철우,점용현,한정희,전윤석,최수영,강대봉,이용묵 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

There were no specific effects for test materials on Sprague-Dawley (S.D.) rats in clinical symptoms, amounts of food intakes, weight changes, laboratory findings, and pathology after whole body l, l-Dichloro-1 -fluoroethane (used as coolant, metal cleaner and solvents) exposure(0, 1,500, 3,000, and 6,000 ppm) for 13 weeks (6 hour/day, 5 days/week). However, the loss of capillary vessels in eyeball (pupil) was observed in a female rat among 6,000 ppm group. Though there was a tendency for MCHC (Mean Corpuscular Hemoglobin Concentration) in rat to be decreased (p<0.05), it was not regarded as abnormal because the values were within normal limits. In asthma-stimulation related evaluations, there was also a tendency for inflammatory cell counts in bronchoalveolar lavages to be increased. But it had no statistical significance, and also no dependency on sex and the exposed concentration. Based on this result, the non observed effect level (NOEL) induced by 1, 1-Dichloro-l -fluoroethene inhalation was evaluated in groups with 3,000 ppm below (S .D. Rats, 13 weeks). Finally, it was concluded that the short term exposal of 1, 1-Dichloro-l-fluoroethane is not considered as a asthma stimulant by inhalation despite of some study limitations such as test animals use and short-term exposure .

수산화칼슘 처리된 Porphyromonas endodontalis Lipopolysaccharide가 다형핵백혈구의 IL-1과 TNF-α 생성에 미치는 영향에 관한 연구

박찬제,박동성,유현미,오태석,임성삼 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.5

Bacterial lipopolysaccharide (LPS) plays a major role in stimulating the synthesis and release of the principal osteoclast-activating cytokines , namely, interleukin 1 and tumor necrosis factor-α from immune cells, Although monocytes/macrophages are the main producers of these cytokines, recent evidence has indicated that polymorphonuclear leukocytes (PMN) have the ability to release IL-1 and TNF-α. Calcium hydroxide has been shown to be an effective medicament in root canal infectionsm, reducing the microbial titre within the canal. It has been proposed that the therapeutic effect of Ca(OH)_2 may also be the result of direct inactivation of LPS. The purpose of this study was to investigate whether treatment of Porphyromanas endodonatalis LPS with calcium hydroxide alters its biological action as neasured by human PMN secretion of IL-1 and TNF-α, and it was compared with Escherichia coli LPS. P.endodonatalis ATCC 35406 was cultured in anaerobic condition, and LPS was extracted using the hot-phenol water extraction method and purified. Purchased E. coli LPS was also purified . 100 ㎍/ml of each LPS in pyrogen free water were incubated with 25mg/ml Ca(OH)_2 at 37℃ for 7 days. The supernatants were subjected to ultrafiltration. And the isolates were lyophilized and weighed. PMNs were obtained from peripheral blood by centrifugation layered over Lymphoprep. The cells were resuspended (4 × 10^6 cells/ml) in RPMI 1640 followed by treatment with various concentrations of LPS (0, 0.1, 1, 10㎍/ml) for 24 hours at 37℃ in 5% CO_2 incubator. The supernatants of cells were collected and the levels of IL-1α, IL-1β and TNF-α were measured by enzyme-linked immunosorbent assay. The results were as follows; 1. The levels of IL-1α, IL-1β, TNF-α from PMN treated with each LPS were significantly higher than those released from unstimulated PMN of the control group (p<0.05). 2. The levels of all three cytokines released from PMN stimulated with each calcium hydroxide treated LPS were significantly lower than those released from PMN stimulated with each untreated LPS (p<0.05). while they were not significantly different from those released from unstimulated PMN of the control group (p>0.05). 3. The levels of secretion or all three cytokines were affected in a dose-dependent manner in PMN stimulated with each LPS (p<0.05), but not in PMN stimulated with each calcium hydroxide treated LPS (p>0.05). 4. The levels of all three cytokines released from PMN stimulated with P.endodontalis LPS were significantly lower than those released from PMN stimulated with E. coli LPS (p<0.05).

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1997)

김재홍,문득곤,김정수,김용준,임동진,박상훈,김희성,이민수,송기훈,김갑형,김형석,성소영,이인섭,김석우,황지환,조창근,김경문,부태성 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

Background : In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. Objective & Methods : For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1997. 99 strains of N. gonorrhoeae were isolated, among which 45(45.5%) were PPNG. Conclusion : The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 45.5% in 1997.

Radiation therapy for gastric mucosa-associated lymphoid tissue lymphoma: dose-volumetric analysis and its clinical implications

Lim, Hyeon Woo,Kim, Tae Hyun,Choi, Il Ju,Kim, Chan Gyoo,Lee, Jong Yeul,Cho, Soo Jeong,Eom, Hyeon Seok,Moon, Sung Ho,Kim, Dae Yong The Korean Society for Radiation Oncology 2016 Radiation Oncology Journal Vol.34 No.3

Purpose: To assess the clinical outcomes of radiotherapy (RT) using two-dimensional (2D) and three-dimensional conformal RT (3D-CRT) for patients with gastric mucosa-associated lymphoid tissue (MALT) lymphoma to evaluate the effectiveness of involved field RT with moderate-dose and to evaluate the benefit of 3D-CRT comparing with 2D-RT. Materials and Methods: Between July 2003 and March 2015, 33 patients with stage IE and IIE gastric MALT lymphoma received RT were analyzed. Of 33 patients, 17 patients (51.5%) were Helicobacter pylori (HP) negative and 16 patients (48.5%) were HP positive but refractory to HP eradication (HPE). The 2D-RT (n = 14) and 3D-CRT (n = 19) were performed and total dose was 30.6 Gy/17 fractions. Of 11 patients who RT planning data were available, dose-volumetric parameters between 2D-RT and 3D-CRT plans was compared. Results: All patients reached complete remission (CR) eventually and median time to CR was 3 months (range, 1 to 15 months). No local relapse occurred and one patient died with second primary malignancy. Tumor response, survival, and toxicity were not significantly different between 2D-RT and 3D-CRT (p > 0.05, each). In analysis for dose-volumetric parameters, $D_{max}$ and CI for PTV were significantly lower in 3D-CRT plans than 2D-RT plans (p < 0.05, each) and $D_{mean}$ and V15 for right kidney and $D_{mean}$ for left kidney were significantly lower in 3D-CRT than 2D-RT (p < 0.05, each). Conclusion: Our data suggested that involved field RT with moderate-dose for gastric MALT lymphoma could be promising and 3D-CRT could be considered to improve the target coverage and reduce radiation dose to the both kidneys.

Radiation therapy for gastric mucosa-associated lymphoid tissue lymphoma: dose-volumetric analysis and its clinical implications

Hyeon Woo Lim,Tae Hyun Kim,Il Ju Choi,Chan Gyoo Kim,Jong Yeul Lee,Soo Jeong Cho,Hyeon Seok Eom,Sung Ho Moon,Dae Yong Kim 대한방사선종양학회 2016 Radiation Oncology Journal Vol.34 No.3

Purpose: To assess the clinical outcomes of radiotherapy (RT) using two-dimensional (2D) and three-dimensional conformal RT (3D-CRT) for patients with gastric mucosa-associated lymphoid tissue (MALT) lymphoma to evaluate the effectiveness of involved field RT with moderate-dose and to evaluate the benefit of 3D-CRT comparing with 2D-RT. Materials and Methods: Between July 2003 and March 2015, 33 patients with stage IE and IIE gastric MALT lymphoma received RT were analyzed. Of 33 patients, 17 patients (51.5%) were Helicobacter pylori (HP) negative and 16 patients (48.5%) were HP positive but refractory to HP eradication (HPE). The 2D-RT (n = 14) and 3D-CRT (n = 19) were performed and total dose was 30.6 Gy/17 fractions. Of 11 patients who RT planning data were available, dose-volumetric parameters between 2D-RT and 3D-CRT plans was compared. Results: All patients reached complete remission (CR) eventually and median time to CR was 3 months (range, 1 to 15 months). No local relapse occurred and one patient died with second primary malignancy. Tumor response, survival, and toxicity were not significantly different between 2D-RT and 3D-CRT (p > 0.05, each). In analysis for dose-volumetric parameters, Dmax and CI for PTV were significantly lower in 3D-CRT plans than 2D-RT plans (p < 0.05, each) and Dmean and V15 for right kidney and Dmean for left kidney were significantly lower in 3D-CRT than 2D-RT (p < 0.05, each). Conclusion: Our data suggested that involved field RT with moderate-dose for gastric MALT lymphoma could be promising and 3D-CRT could be considered to improve the target coverage and reduce radiation dose to the both kidneys.

Comparative Efficacy and Bioequivalence of Novel H1-Antihistamine Bepotastine Salts (Nicotinate and Salicylate)

Lim, Duck Soo,Youn, Yoo Seok,Kwack, Seung Jun,Kwak, Hyo Min,Lim, Seong Kwang,Kim, Ji Yun,Um, Yoon Mi,Lee, Jung Dae,Hyeon, Ji Hyeon,Kim, Yeon Joo,Kim, Hyung Sik,Lee, Byung-μ Taylor Francis 2014 Journal of toxicology and environmental health. Pa Vol.77 No.22

拱辰丹 方義에 대한 고찰 : 『東醫寶鑑』의 내용을 중심으로

임석현(Lim Seok-hyeon)(林錫賢),정창현(Jeong Chang-hyun)(丁彰炫),장우창(Jang Woo-chang)(張祐彰),추면(Zou Mian)(鄒冕),백유상(Baik Yousang)(白裕相) 대한한의학원전학회 2020 대한한의학원전학회지 Vol.33 No.4

Objectives : 본 연구는 임상에 유용하게 활용되기 위해 『東醫寶鑑』의 拱辰丹 내용을 고찰하여 拱辰丹의 方義와 적용 대상과 작용 기전을 밝혀내는 것이 목표이다. Methods : 먼저 한의학 원문 데이터베이스를 활용하여 拱辰丹 관련 자료들을 검토하였다. 또한 『是齋百一選方』과 『東醫寶鑑』의 관련 내용을 분석하고, 拱辰丹의 구성 약재들에 대한 효능을 『東醫寶鑑』과 『중약대사전』을 중심으로 검토하였다. Results & conclusion : 『東醫寶鑑』에서 拱辰丹을 적응증을 연령대와 장부별로 검토한 결과, 연령대별 拱辰丹의 적용 대상은 先天 稟賦가 부족한 소아와 壯年에게는 사용할 수는 있으나 노인에게는 가장 적합하다고는 할 수 없었다. 장부별로는 『東醫寶鑑』 ｢虛勞門｣의 의하면 拱辰丹은 水升火降의 기전에서 水에서 火로 상승시키는 작용이 있어, 肝虛로 인하여 精血이 耗損을 補하면서 기운을 끌어올려 불안하며 두려워하는 精神 증상을 치료할 수 있었다. 따라서 拱辰丹은 젊은 나이에 체력이 약하면서 겁먹고 의욕이 없는 상태에 사용하는 것이 가장 적합하다고 할 수 있다. Objectives : This paper aims to lay out the meaning of Gongjindan, its indications and mechanisms based on relevant contents in the 『Donguibogam』, for better clinical application. Methods : First, Gongjindan related contents were searched in the medical classics database. Next, contents from the 『Shizhaibaiyixuanfang』 and the 『Donguibogam』 were analyzed. Finally, the properties of the Gongjindan ingredients were examined based on the 『Donguibogam』 and the 『Zhongyaodacidian』. Results & Conclusions : Examination of its indications according to the 『Donguibogam』 in terms of applicable age and viscera/bowels, Gongjindan could be applied to children with constitutional insufficiency and elder generations, but it could not be said to be most appropriate for older generations. In regards to viscera/bowels, Gongjindan sends water upwards into fire in the water-rising-fire-descending mechanism, which makes it applicable to symptoms of anxiety and fear by tonifying the consumed Jing and Blood caused by Liver deficiency. To summarize, those who would most benefit from Gongjindan are young adults in weak, fearful and lethargic conditions

Thermal Behavior of Hot Strip in Finishing Mill of Hot Rolling Process

Jong-Hyeon Lee,Jeong-Seok Kang,Jin-Taek Kim,Byung-Joon Baek,Sung-Hyuk Lim,Jun-Seok Lim,Hyeong-Jin Kim 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.10

To apply efficient rolling conditions in the finishing rolling process, a correct prediction of the behavior of the strip temperature is required. This study is focused on the temperature distribution of the strip temperature from FET to FDT of the actual finishing rolling process. The heat transfer phenomena generated during the finishing rolling process, such as heat generation, cooling and conducting, were modeled in this study using the finite difference method. Using various heat transfer phenomena generated in the finishing rolling process and the correlation formula of spray cooling coefficient, the calculation accuracy was improved and a simulator to predict the temperature distribution of strip was prepared. The calculated result is compared to the online model used in the actual industry.