Hepatic Cirrhosis Occurring in a Young Woodchuck(Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus(WHV)

Kyu-shik Jeong,정원일,Jae-yong Chung,Mi-young An,Chae-yong Jung,Gyoung-jae Lee,Jong-soo Kang,Byeong-cheol Kang,Young-heun Jee,Bruce H Williams,Young-oh Kwon,Da-hee Jeong 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

Cirrhosis Occurring in a Young Woodchuck (Marmota monax) Due to Vertical Transmission of Woodchuck Hepatitis Virus (WHV)Da-hee Jeong, Won-il Jeong, Jae-yong Chung, Mi-young An, Chae-yong Jung, Gyoung-jae Lee1, Jong-soo Kang1, Byeong-cheol Kang2, Young-heun Jee3, Bruce H Williams4, Young-oh Kwon5 and Kyu-shik Jeong*College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Korea1Shinwon Scientific Co., LTD, Research Institute, Suwon, Korea2Clinical Research Institute, Seoul National University, Seoul 110-744, Korea3College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea

Histone deacetylase 3 is selectively involved in L3MBTL2-mediated transcriptional repression

Yoo, Jung-Yoon,Choi, Kyung-Chul,Kang, HeeBum,Kim, Young Jun,Lee, Jeongmin,Jun, Woo Jin,Kim, Mi-Jeong,Lee, Yoo-Hyun,Lee, Ok-Hee,Yoon, Ho-Geun Elsevier 2010 FEBS letters Vol.584 No.11

<P><B>Abstract</B></P><P>This is the first report that L(3)mbt-like 2 (L3MBTL2) specifically interacts with the histone deacetylase domain of histone deacetylase 3 (HDAC3) via its MBT domain. Here, we show that L3MBTL2 selectively interacts with HDAC3, but not other class I HDACs. An in vitro peptide-binding assay demonstrated the specific association of HDAC3 with methylated histone-K20 tail and L3MBTL2. Furthermore, depletion of HDAC3 resulted in a decrease of methylated K20-H4, as well as an increase in acetylated histone H3. Consequently, HDAC3 knock-down selectively suppressed L3MBTL2-mediated transcriptional repression. Taken together, our results reveal the concerted action of both HDAC3 and L3MBTL2 in histone deacetylation and methylation-dependent transcriptional repression.</P><P><B>Structured summary</B></P><P>MINT-7719975: <I>L3MBTL2</I> (uniprotkb:Q969R5) and <I>HDAC3</I> (uniprotkb:O15379) <I>colocalize</I> (MI:0403) by <I>fluorescence microscopy</I> (MI:0416)</P><P>MINT-7719941, MINT-7719921: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>binds</I> (MI:0407) to <I>HDAC3</I> (uniprotkb:O15379) by <I>pull down</I> (MI:0096)</P><P>MINT-7719991: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>anti bait coimmunoprecipitation</I> (MI:0006)</P><P>MINT-7719958: <I>L3MBTL2</I> (uniprotkb:Q969R5) <I>physically interacts</I> (MI:0915) with <I>HDAC3</I> (uniprotkb:O15379) by <I>anti tag coimmunoprecipitation</I> (MI:0007)</P><P>MINT-7719897: <I>HDAC3</I> (uniprotkb:O15379) <I>physically interacts</I> (MI:0915) with <I>L3MBTL2</I> (uniprotkb:Q969R5) by <I>two hybrid</I> (MI:0018)</P>

심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

김영랑 시의 정신세계

강영미(Kang Young-mi) 한국시학회 2004 한국시학연구 Vol.- No.10

Kim Young-rang has been known as poet who commanded elaborated type, meter, soft tone harmony of voice. Manufacture do poetry which have self-regulating structure through conscious calculation and materialize own inside sensuously. And his interest about shi-jo and pansori is reflected in production of poetry. He creates four line poetry criticizing existent poetry style(shi-jo and pansori and so on)in poetry´s circles in the 1930s that form of free poetry catches seat already, and plays a variation four line poetry learning forms and groped new modernistic poetry form. He established peculiar sensitivity of individual into compensation of shape and create chard free verse of new form. These features that shows in poetry form that defend self from colony actual broken external order is revealed can be seen. It is because construct space of imagination and found psychological pupil of the eye in to close loss persimmon formed on inside looking away actuality that can not accomplish own dream. Therefore, that appears in his poetry sensual and the trillion brazier fortune worlds can see that is product of injustice mind to look away colony actuality. Kim Young-rang did not compromise in colony actuality and could keep own inside to be upright through writing such poetry.

미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

남성화를 보이는 여성에서 발견된 난소의 Steroid Cell Tumor 1예

조인호,정대훈,박영미,서영진,손영실,정철회,강영미,정수전,김영남,이경복,성문수,김기태 인제대학교 2006 仁濟醫學 Vol.27 No.-

Steroid cell tumor is a rare ovarian sex cord-stromal tumor which accounts for 0.1% of all ovarian tumors. Until now, only 4 cases have been reported in domestic literatures. Steroid cell tumor often secrets testosterone and presents virilization in adult women or precocious puberty in children. Treatment is often performed by surgical removal, adjuvant chemotherapy and radiation, but completely accepted treatment was not existed. We experienced a case of steroid cell tumor, which was manifested by typical virilization in a 43-year old patient, who was previously performed hysterectomy and unilateral oophorectomy. So, we present with a brief review of the literatures.

5년간 특수건강진단기관 분석정도관리 결과 분석

강성규,양정선,이미영,박인정,정호근 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목 적 : 1980년대 후반부터 산업보건에서 혈중 및 요중 중금속농도 분석의 정확성 문제가 크게 부각되었다. 같은 시료를 가지고 분석기관마다 보이는 커다란 오차로 인해 직업병 진단 결과에 대한 신뢰성을 크게 감소시켰다. 이에, 노동부에서는 1992년부터 특수건강진단 기관에 대한 생체시료 분석정도관리 프로그램을 시작하였다. 본 연구는 1995년부터 시작된 생체시료 분석정도관리 5년간의 결과를 보고하고, 현재 생물학적모니터링으로 사용되는 항목과 빈도를 조사하고 이 항목의 정도관리 참여율을 비교하여 산업보건 관계 연구나 직업병 관리에 참고할 수 있도록 하기 위해 실시하였다. 방 법 : 생체시료 분석정도관리는 무기와 유기분석분야로 나누어 일년에 전후반기 2회 실시하고 있으며, 지정항목과 자율항목으로 구분하고 있다. 지정항목인 혈중 납과 요중 마뇨산은 분야별로 한 번에 3개 농도수준을 주어 2개 이상이 기준값의 ±15%내외의 범위에 들면 적합한 것으로 판정하고 있으며, 자율항목은 2개의 시료를 주어 2개 모두 기준실험실 평균값의 ±3SD 내외의 범위에 들면 적합한 것으로 판정하고 있다. 결 과 : 지정항목인 혈중 납과 요중 마뇨산 분석은 평균 적합률이 각각 89%, 90% 이었고, 대학기관, 비영리법인, 종합병원, 사업장 자체기관별로 차이는 없었으나 신규 기판의 적합률 70% 수준으로 상대적으로 낮았다. 자율항목의 적합률은 평균 50% 수준이었고, 저농도보다는 고농도의 적합률이 떨어졌다. 국내에서 가장 많이 분석되고 있는 항목은 요중 마뇨산과 혈중 납이었고, 혈중 및 요중 망간도 많이 분석되고 있었다. 요중 삼염화물, 페놀, 만델산, 카드윰, 메칠마뇨산, 크롬 등도 많이 분석되고 있는 항목이었다. 혈중 망간, 요중 수은, 요중 NMF는 정도관리에서 적합판정을 받은 기관의 숫자보다 실제 분석을 하고 있는 기관의 숫자가 많았다. 결 론 : 지난 5년간의 생체시료 분석정도관리 결과 우리나라 분석실험실의 분석능력은 향상되고 있으나, 자율항목에 대한 분석능력은 아직 미흡한 것으로 나타났다. 그리고, 아직 일부 일부 항목에 대해서는 외부 정도관리 없이 분석되고 있는 경우도 있었다. Objective : The accuracy of analytical results of blood and urine heavy metals came out to the main issue on occupational health from late eighties. The discrepancy of the results for same samples from different laboratories made the diagnosis for occupational diseases be unreliable. Therefore, a quality control program for analysis of samples taken from workplace had been introduced in Korea since 1992. This study aims to show the quality control program for analysis of blood and urine samples and its proficient rates from 1992 to 1999 and to know how they have been being used in occupational health. Methods The quality control program runs twice a year with mandatory items of blood lead and urine hippuric acid and voluntary items of blood cadmium and manganese and urine mandellic acid and methyl hippuric acid. Participant laboratories are receiving three levels for each items and two out of three samples have to be qualified for being a profi-cient laboratory for the item. The acceptable range of blood lead and urine hippuric acid is ± 15% and that of the others is within 3 SD (standard deviation) from the reference values. Results : The proficient rates of blood lead and urine hippuric acid was 89%, 90%, repectively, however those of the other voluntary items have been from 51% to 62%. The proficient rates of urine mercury and urine N-methylformamide (NMF), which are introduced since 1999, were very poor. Urine hippuric acid and blood lead were analyzed frequently for the purpose of biological monitoring conducting by special health examination organizations. Urine and blood manganese and urine metabolites of trichloroethylene, urine phenol, methylhippuric acid and cadmium were followed. Conclusions : In conclusion, the quality control program for biological monitoring has dramatically improve the ability of analysing blood and urine samples and eventually con-tributes to diagnose occupational diseases and to prevent occupational poisoning. However, some biological monitoring data, such as urine manganese, mercury and NMF, have been still reported from laboratories that were not accepted as a proficient laboratory.

중환자의 욕창 예방 연구 : 욕창 예방 QI팀을 중심으로

강소영,최은경,김진주,주미정 한국의료QA학회 1997 한국의료질향상학회지 Vol.4 No.1

Background : A pressure sore was defined as any skin lesion caused by unrelieved pressure and resulting in damage to underlying tissue. The health care institutions in the United States were reported the incident rate of pressure sores ranging from 6 to 14%. United states were reported the incident rate of pressure sores in patients rate of pressure sore. Also, Annual expenditures for the care of pressure sores in patients in the United States have been estimated to be $7.5 billion; furthermore, 50 percent more nursing time is required to care for patients with pressure sore in comparison to the time needed to implement preventive measures against pressure sore formation. However, In Korea, there were little reliable reports, or researches, about incidence rates of pressure sore in health care institution including intensive care unit and about the integrated approach like CQI action team for risk assessment, prevention and treatment of pressure ulders. Therefore, this study was to develop pressure sore risk assessment tool and the protocol for prevention of pressure sore formation through CQI action team activities, to monitor incident rate of pressure sore and the length of sore formation for patients at high risk, and to approximately estimate nursing time for sore dressing during research period as the effect of CQI action team. Method : CQI action team in intensive care unit, launched since early 1996, reviewed the literature for the standardized risk assessment tool, developed the pressure sore assessment tool based on the Braden Scale, tested its validity, compared on statistics including incidence rate of pressure sore for patients at high risk. Throughout these activities, CQI action team was developed the protocol.called as St. Marys hospital Intensive Care Unit Pressure Sore Protocol, shifted the emphasis from wound treatment to wound prevention, After applied the protocol to patients at high risk, the incident rate and the period of prevention against pressure development were tested with those for patients who received care before implementation of protocol by Chi-square and Kaplan-Meier Method of Survival Analysis. Result : The CQI action team found that there was significant difference of incidence rate of pressure sores between patients at high risk( control group) who received care implementation of protocol (p<.05). 25% possibility of pressure sore formation was shown for the patients with 6th hospital day in ICU in control group. In experimental group, the patients with 10th hospital day had 10% possibility of pressure sore. Therefore, there was significant difference(p< .05) in survival rate between two groups. Also, nursing time for dressing on pressure sore in experimental group was deceased as much as 50% of it in control group. Conclusion : The collaborative team effort led to reduced incidence, increased the length of prevention against pressure sore, and declined nursing care times for sore dressing. However, there have had several suggestions for future study. The preventive care system for pressure sore should be appleed to patients at moderate, or low risk throughout continuous CQI team activities based on Bed sore Indicator Fact Sheet. Hospital-wide supports, such as incentives, would be offered to participants for keeping strong commitment to CQI team. Also, Quality Information System monitoring incidents and estimation cost of poor quality. like workload(full time equivalence) or financial loss, regularly in a hospital has to be developed first for supporting CQI team activities as well as empowering hospital-wide QI implementation. Being several limitations, this study would be one of the report cards for the CQI team activities in intensive care unit of an acute hospital and a trial of quality improvement of health care in Korea.

해운대 달맞이 언덕에 있어서 바다조망경관의 역사적 변천에 관한 연구

강영조,신수안,조미나,이현주,박은희 동아대학교 건설기술연구소 2000 硏究報告 Vol.24 No.1

The purpose of this study is to clarify the historic transition of the prospects on Haeundae dalmaji hill. Dalmaji hill is well known for a character of configuration on the ground with the scene of the sea. We will infer that how did it change the prospects of Dalmaji hill's sea through the analyzing of the preset situation and location of building on the hill. (1) We investigated the stories of the building, the history, the business condition. (2) We collect the twenty four view points which is able to have seascape on the hill, being easily approaching to the road, and condition of changing the building. (3) We clarified the transition of seascape prospect and considered the characteristics of the six types from which were divided the condition of seascape prospect.

흰쥐 뇌의 냉동손상 후 항원제공세포와 신경교원섬유산단백의 발현 양상

강미영,김강련 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.1

Background : Traumatic brain injury is a multifaceted injury that involves direct mechanical damage, intraparenchymal and subarachnoid hemorrhage, breakdown of the blood-brain barrier, excitotoxicity, and ischemia. Even though much investigations were performed, acceptable mechanical informations were rare. The aim of this study was to reveal the migration of antigen presenting cells associated with inflammation and the expression pattern of GFAP associated with gliotic scars in cerebral cortex of rats after cryoinjury. Material & Method : A total of 18 male Sprague-Dawley rats weighing 300 g, 2 months old, were used throughout the experiments. To injure the brain, rats were anesthetized for surgery with 3.5% chloral hydrate(1 ㎖/100 g, intraperitoneally); the frontal bones were exposed by elevating the skin; and craniectomies were performed adjacent to the central suture, midway between lambda and bregma. A cryoinjury was then created by applying a cold probe (3-mm-diameter steel rod chilled in liquid nitrogen) to the left frontal cortex (ipsilateral cortex) for 1 minute. Rats were sacrificed at 6 hours, 1, 4, 7 and 14 days postsurgery (n=3, per time point), and three rats were sacrificed as normal controls. Serial brain cryosections were made by cryostat. For immunohistochemistry, brain tissue sections were allowed to react with mouse anti-rat MHC class II antibody(1:500), mouse anti-rat ED2 antibody(1:500), and mouse anti-rat GFAP antibody(1:200). Results : MHC class II positive dendritic cells were absent in normal cerebral cortex parenchyme, but were more 16 times number in injured rats at 7 days after cryoinjury. ED2 positive macrophages were rare in normal cerebral cortex parenchyme, and were more 14 times number in injured rats at 4 days after cryoinjury. GFAP positive astrocytes were not observed in normal rats, and increased suddenly at 4 days rats after cryoinjury. GFAP positive neurons were not expressed in normal rats, and in cryoinjured rats. Conclusion : In conclusion, the above results suggest that the increases of the number of the two kinds of the antigen-presenting cells affect cell-mediated immune responses and phagocytosis, and GFAP positive cells attends in the formation of gliotic scars.