Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sohn, Sea-Hwan,Cho, Eun-Jung,Jang, In-Surk The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.4

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

에탄올성 간손상 흰쥐의 유해산소 생성효소계에 미치는 칡 추출물의 영향

조수열,김명주,박은미,박지윤,장주연 한국노화학회 2001 한국노화학회지 Vol.11 No.1

6차와 개편된 7차 교육과정 중학교 과학 교과서 생물영역에 대한 비교 분석

장은영,조현욱,김종홍 順天大學校 師範大學 附屬 科學敎育硏究所 2002 科學과 敎育 Vol.10 No.-

The purpose of this study is to compare and analyze the biology area of the 6th and the revised 7th curriculum in the middle school The 6th curriculum emphasized the research activities. It also gave emphasis on the biological materials in our surroundings, field studies, open questions and various applications and the participation in scientific events, while the new curriculum points on the ability to group either between classes or within classes. In―depth and supplementary differentiated curriculum in the 7th curriculum makes "level―based differentiated curriculum" possible and stresses the synthetic research activities, which deal with fundamental notions and the research processes. Looking into the degree of emphasis for each scientific area, the new textbooks have 12 chapters, but the old textbooks have 4 chapters. The allotment of each scientific area is almost same in the new textbooks, through matter (22.7%) takes up less pages than other areas. In the old textbooks, the number of pages allotted for biological subjects is 28.4% and takes up more pages than other areas. The content dealing with biological area comprises 67.5 pages of the new textbooks and 77 pages of the old textbook. The items analyzing constituents for the research activities appear in the new textbooks. These constituents are planning observing and assuming, investigating, reading and building ideas. The common experiments in both textbooks are 22 in the new textbooks, and 11 in the old textbooks.

2.5-헥산디온에 노출된 근로자에게서 발생한 말초 신경병증

조성용,장용석,최은경,김진석,유재영,우극현,최태성 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.1

목적: 영상음향장비 제조업체에서 2,5-헥산디온을 취급한 근로자에게서 발생한 다발성 말초 신경병증 환례를 보고하고, 동일 공정근로자들의 말초 신경기능을 조사하고 자 한다. 방법: 환례에 대해 방사선학적 검사, 소변 및 혈액 검사, 신경전달속도 검사, 작업환경 측정을 하였으며, 2,5-헥산디온의 노출을 중단시킨 후 5개월 후에 다시 신경전달속도 검사를 하였다. 동일공정 근로자들의 영향평가에서는 2,5-헥산디온에 노출된 13명의 남자 근로자를 노출 군으로, 해당 물질에 노출된 적이 없는 5명의 남자 근로자를 비노출군으로 선정하여 검사를 하였다. 결과: 환례의 신경전도 검사결과 양측 척골신경과 정중 신경에 이상소견을 보였으며, 호전여부를 관찰하기 위하여 5개월 후 추적검사를 실시한 결과 증상 및 신경전달속도 검사결과가 호전되었다. 동일공정 근로자들의 영향평가에서 노출군의 정중 신경과 척골신경이 비노출군에 비하여 유의하게 감소되어 있었다. 감각 신경의 진폭의 경우, 노출군의 정중 신경과 비복 신경이 감소되었으며, 운동 신경 전도속도에서는 노출군의 비골신경이 유의하게 감소되어 있었다. 말단 잠복기에서는 노출군의 정중신경, 척골신경, 비골신경, 경골신경이 비노출군에 비해서 유의하게 연장되어 있었다. 결론: 환례의 증상과 신경전달속도 검사의 이상소견은 2,5-헥산디온에 의한 말초신경병증에 부합되었다. 동일공정 근로자 건강영향평가에서 신경전달속도 검사결과 노출 군과 비노출군간의 유의한 차이가 있었는데, 이를 통해 2,5-헥산디온이 근로자들의 신경독성에 영향을 줄 수 있다는 것을 확인할 수가 있었다. Objectives: This study is a case report of 2,5-hexanedione induced occupational peripheral polyneuropathy. We also investigated the peripheral nerve function of all workers who had been exposed to 2,5-hexanedione in the same process. Methods: In June, 2006, a 2,5-hexanedione exposed worker complained of both hand numbness. He received neurologic, radiologic, laboratorial and electrophysiologic evaluation, including measurements of workplace environment. Five months after cessation of exposure to 2,5-hexanedione, a follow-up electrophysiologic examination was done. We evaluated the peripheral nerve function of 2,5-hexanedione exposed workers by comparing 13 male 2,5-hexanedione exposed workers who were in same company with the patient and 5 male workers who had not been exposed to 2,5-hexanedione. Results: Under electrophysiologic examination, there were abnormalities in sensory and motor nerve velocity, terminal latency, and F-latency of both median nerve and ulnar nerve. After 5 months, the patient symptoms and the results of follow-up electrophysiologic examinations were improved. Comparing the 2,5-hexanedione exposed group with the unexposed group, the sensory nerve velocity of the median and ulnar nerves in the exposed group was decreased. The motor nerve velocity of the peroneal nerve, and sensory nerve velocity of the median and sural nerves were decreased. Terminal latency of median, ulnar, peroneal, and tibial nerves in the exposed group were increased compared with the unexposed group(<0.05). Conclusions: 2.5-hexandione can induce peripheral polyneuropathy in male workers.

韓國人의 血液 및 各 臟器組織中 靑酸鹽의 微量分布에 關한 硏究 : Concerning Micro Distribution of Cyanide in Human Organs

張成吉,金恩浩,金英雲,李鎭勳,趙承衍 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

심전도의 디지털 기록화 모듈과 개인용컴퓨터 저장/분석시스템의 개발

조명찬,김기석,배장환,연태진,김동운,이태수,전은석,김유진 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: 현재 우리나라의 의료분야에서는 정보기술의 발전으로 처방 전달 시스템과 전자차트의 활용이 상용화 되고 있으나 병원내에서 가장 많이 처방되고 검사되는 12-유도 심전도의 디지털화와 이에 연계된 심전도 결과의 개인용 컴퓨터로의 저장, 인터넷을 이용한 병원으로의 전송이나 타 기관에서의 심전도 결과의 동시 관찰 등에 관한 기술 등은 개발되지 못한 상태이다. 이에 본 연구에서는 심전도를 디지털화하며 저장이 가능한 PC 모듈을 개발하고 심전도 결과를 병원 전산 시스템에 포함시켜 병원내외에서 편리하게 조회, 검색할 수 있는 디지털 인터페이스를 개발하려 하였다. 재료 및 방법 : 낮은 전력에서 잡음특성이 우수한 대역여파기와 증폭기를 설계하였으며 각종기능이 내장된 고신뢰성 프로세서를 채택하였으며 전원 잡음이 고려된 필터와 전원 회로 설계로 하드웨어를 개발하였다. MSC 6.0과 Visual Basic 6.0을 바탕으로 하여 active X 모듈화를 유도하고 조회용 PC에서의 복원과 PACS로의 연동을 통해 순환기내과용 PACS 개발이 가능하도록 소프트웨어를 준비하였다. 결과: 심전도 검사자가 쉽게 장착, 검사가 가능한 소형화된 측정 모듈을 개발하였고 극저전류에서 동작이 가능하였으며 우수한 데이터의 압축 및 복원률을 구현하였다. 결론: 이번 심전도 디지털 모듈의 개발로 기존의 감광지에 의존하던 심전도 결과의 보존과 보관공간에 의한 경제적인 손실을 상당 부분 해결할 수 있으며 OCS에 연계된 결과로 인해 원내에서의 다면적 진료가 가능하다. 이번 연구에서 개발된 기술은 향후 디지털화된 심전도 기록장치와 같은 고부가 의료장치의 국산화와 광파일 의무기록 시스템이나 검사물의 무선 전송에 채용되어 의료 전달 체계나 응급체계의 발전에 기여하리라 사료된다. Purpose : The progressed communication technology commercialized the order communication system ( OCS) and the electric medical records( EMR) in the field of medicine in Korea. However, technologies of digitalization such as recording to personal computer, transfer to internet and simultaneous observation of electrocardiogram ( ECG) recorded at the different center were not realized yet. This study tried to invent personal computer module that can digitalize and record of the ECG and design the digital interface that can approach and search digitalized ECG in various part of hospital simultaneously. Materials and Methods : We had designed high-fidelity digital filter and amplifier which can work in very low voltage, had selected multifunctioned microprocessor. Activation X modulation was conducted by MSC 6.0 and Visual Basic 6.0. Softwares were prepared for the transfer of ECG informations from terminal PC to PACS and cardiologic PACS was designed for future application. Results : We have developed the small, portable, very low voltage managed ECG module and high quality digitalized information compression software in OCS and PACS. Conclusion : This invented ECG digital archive module can save hospital cost by substitution of ECG papers for digital storage or on-line storage, therefore ECG data can be handled in multisector of the hospital and through telemedicine. This technology can be applied to domestic ECG digitalizing device development and effective medical transfer system by adoption cordless medical information delivery.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

곡류가루 첨가가 소시지의 품질 및 저장성에 미치는 영향

장선문,임지숙,조은자 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.3

To investigate the effect of cereal powders on the quality and storage characteristics of sausage, Aw, pH, microbial count, TBA value, VBN value, textual and sensory characteristics were examined. Water activity tended to decrease as storage time prolonged and all the samples showed higher Aw's than that of the control. During the storage period, pH values of all the samples decreased. L, a and b values had a tendency to decrease during the storage period. During the four weeks storage at 4℃, TBA and VBN values of all the sample showed increasing tendency and were lower than those of the control. Total plate counts of bacteria, the number of lactic acid bacteria and coliform bacteria counts increased as the storage time prolonged. Texture characteristics of all samples increased up to 1 week storage and decreased subsequently. The sensory scores of all the samples decreased as the storage time prolonged.

밀도 기반의 k-최근접 질의 처리

장인성,한은영,조대수 한국지리정보학회 2003 한국지리정보학회지 Vol.6 No.4

공간 데이터베이스 관리시스템에서 제공하는 공간 질의는 많은 디스크 참조와 CPU 처리시간을 필요로 한다. 이 중에서 k-최근접 질의는 많은 디스크 참조를 요구하는 질의로써 지금까지 많은 연구가 이루어져 왔다. 트리 구조의 색인을 사용하는 k-최근접 질의 처리방법은 조건을 만족하지 않는 노드를 가지치기 기법을 사용하여 노드 방문횟수를 줄인다. 그러나 이 방법은 가지치기 과정에서 불필요한 디스크 참고가 발생하여 성능을 저하시키는 단점을 가지고 있다. 본 논문에서는 가지치기 기법 대신 주어진 k개의 최근접 객체가 존재할 영역을 미리 예측함으로써 디스크 참조횟수를 줄이는 방법을 제시한다. 이 영역을 예측하기 위해서 본 연구에서는 데이터 분포에 대한 밀도를 이용하였다. 실험에 의하면 이러한 방법은 기존의 가지치기 기법을 이용한 방법에 비해서 최고 22%, 평균 7% 정도의 디스크 참조횟수의 감소 효과가 있음을 알 수 있다. Spatial database system provides many query types and most of them are required frequent disk I/O and much CPU time. κ-NN search is to find κ-th closest object from the query point and up to now, several κ-NN search methods have been proposed. Among these, MINMAN distance method has an aim not to access unnecessary node by adapting pruning technique. But this method accesses more disks than necessary while pruning unnecessary nodes. In this paper, we propose new κ-NN search algorithm based on density of object. With this method, we predict the radius to be expected to contain κ-NN objects using density of data set and search those objects within this radius and then adjust radius if failed. Experimental results show that this method outperforms the previous MINMAX distance method. This algorithm visit less disks than MINMAN method by the factor of maximum 22% average 7%.

MHC class Ⅰ분자들에 의해 제시되는 Epitope을 인지하는 CD8+ T림프구의 결핵균 감염에 대한 면역반응의 연구 : 결핵환자와 PPD+ 건강개체에 존재하는 결핵균 항원에 특정한 CD8+ T세포 Evidence of M. tuberculosis Specific CD8+ T Cells in TB Patients and PPD+ Healthy Individuals

조장은,이경화,박승규,천선희,조상래,조성애 Official Journal of the Korean society for immunol 2003 Immune Network Vol.3 No.3