직업적 연폭로가 일부 신경행동학적 검사수행에 미치는 영향

안익수,안현철,김용배,리갑수,김화성,황규윤,최은,장봉기,이성수,안규동,이병국 순천향대학교 산업의학연구소 1999 순천향산업의학 Vol.5 No.1

In order to investigate that blood lead levels of lead exposed workers is associated with decreased performance in neurobehavioral tests in adults, we investigate 78 workers in a battery industry. Three items among neurobehavioral core test battery of World Health Organization, including digit span, Santa ana dexterity, digit symbol were administered to workers occupationally lead exposed workers by operational guide for the WHO neurobehavioral core test battery. Blood lead (PbB) and zinc protoporphyrin in whole blood (ZZP) were selected as an index of lead exposure. Detailed occupational history such as exposed agent, age, work duration, smoking and drinking habits, were recorded as confounding factors. After controlling age, the results of digit span and digit symbol among the neurobehavioral tests were significant contributed by PbB.

溫床用上의 理化學的 特性이 幼苗의 系質에 미치는 影響 : Park, Hwa-Sung & Kwang-Soo Kim 溫上의 異化學的 特性

朴華性,金光秀 全南大學校 農漁村開發硏究所 1982 農業科學技術硏究 Vol.17 No.1

本 實驗은 施設園藝地帶인 光山郡, 順天市, 康津郡 等에서 葉菜類와 果菜類의 育苗用 床土를 試料로 22点 分讓받아 수행하였으며 結果는 다음과 같다. 1.床土의 助成과 性質에 關與하는 가장 重要한 因子는 有機物含量인데 有機物含量이 높아짐에 따라 假比重과 眞比重은 낮았고 空隙率, 水分含量, 全窒素, 置換容量은 높아진다. 2. 葉菜類의 床土는 有機物含量이 많은 반면 果菜類床土의 경우는 그보다 낮게 나타났다. 봄결구배추床土의 有機物含量은 8.85~9.19%의 範圍로 別變化가 없었다. 3. 床土의 pH는 4.9~7.2의 範圍에 있었으며 大部分이 pH 7±1로서 中性에 가까웠다. 4. 置換性 K는 7点이 1.0m.e. 以上이었고 15点이 1.0m.e. 以下로 나타났다. 5. 苗의 바람직한 生育을 위해 P.K.의 量을 規定짓기는 어려우나 床土의 試料內에는 相當量의 養分이 含有되 있는 것을 알 수 있다. This experiment was intended to know the effects of physical and chemical properties of the excellent composts. Twenty two samples were collected from plastic covered greenhouse zone in chonnam province. The results obtained are as follows; 1. One of the most important factors in relation to the texture and other properties of the composts was the organic matter content. The higher the organic matter content were the lower the specific gravity and the apparent specific gravity and the higher the porosity, moisture content, total nitrogen and exchangeable capacity. 2. The composts for raising leaf vegetable seedlings contained higher levels of organic matter while the ones for fruit vegetable contained rather less. The organic matter contents in the composts for spring heading Chinese cabbage were not variable ranging from 8.85 to 9.19 percent. 3. Hydrogen-ion concentrations of the composts were in the range of pH 4.9~7.2 but most of them were in pH 7±1 or nearly neutral in their reaction. 4. Seven samples contained more than l.0m.e. of exchangeable potassium, and fifteen of them contained lower than l.0m.e. of it. 5. It was not clear how high of P or K levels were sufficient for the excellents in some of the samples, at least, were so high, that they seemed to contain surplus amounts of fertilizers.

납 작업자들에서 ALAD 유전자형과 신기능과의 연관성

이성수,김진호,김남수,김화성,안규동,이병국 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

목적 : δ-aminolevulinic acid dehydratase (ALAD)는 다형질성 (polymorphism)의 특성이 있어 유전형질에 따라 남의 인체영향이 다르다고 보고 된 바 있다. 본 연구에서는 이 유전인자의 다형질성이 신기능 지표들에 미치는 영향을 알아보고자 하였다. 방법 : 조사대상으로는 납 취급 사업장에서 납 작업에 종사하는 935명의 남자 근로자들과 동일한 사업장에서 근무하는 비 납 작업자 87명을 선정하였다. 단면적 신기능지표로는 BUN, 혈청 creatinine, 혈청 uric acid, 요중 총단백을 측정하였으며, 납 폭로 지표로는 혈중 납량을 측정하였다. 또한 개인 일반 변수로서 체중, 연령, 직력과 흡연 및 음주습관을 조사하였다. 결과 : 납 작업자 군의 혈중 납의 평균(표준편차)은 25.4±10.9 ㎍/㎗이었으며, 비 납 작업자 군의 평균(표준편차)은 10.1±2.8 ㎍/㎗로서 양 군 간에 유의한 차이가 있었다(p<0.05). 납 작업자 군에서 이형유전형질인 ALAD2 유전자형을 가진 납 작업자는 99명으로 전체의 10.6%를 차지한 반면, 비 납 작업자 군에서는 ALAD2를 가진 근로자는 4명으로 전체의 4.6%이었으며 양 군 간의 유의한 차이는 없었다. 동형유전형질인 ALAD1 유전자형 군의 혈중 납의 평균(표준편차)은 23.9±11.4 ㎍/㎗ 이었고 ALAD2 유전자형 군의 평균(표준편차)은 25.8±10.7 ㎍/㎗이었으나 양 군 간에 유의한 차이는 없었다. 신기능 지표들인 BUN, 혈청 creatinine, 혈청 uric acid 및 요중 총단백의 평균은 납 작업자 군이나 비 납 작업자 군에서 유의한 차이가 없었으며, 유전형질구분에 따른 차이도 없었다. 연령, 직력, 체중, 음주여부, 흡연여부 및 혈중 납을 통제한 후 BUN의 중위수를 기준으로 중위수이상 유무를 종속변수로 하고 관련변수들을 통제한 후 실시한 logistic 분석에서는 ALAD2 유전자형의 odds ratio가 0.59 (95% 신뢰구간 : 0.38-0.91)이어서 ALAD2 유전자형 군에서 ALAD1 유전자형군보다 41% 적게 중위수를 가질 것으로 판명되었다. 혈청 creatinine의 중위수를 기준으로 중위수이상 유무를 종속변수로 하고 관련변수들을 통제한 후 실시한 logistic 분석에서는 ALAD2 유전자형의 odds ratio가 0.64 (95% 신뢰구간 : 0.40-0.98)이어서 ALAD2 유전자형 군에서 ALAD1 유전자형 군보다 36%가 적게 중위수를 가질 것으로 판명되었다. 결론 : 이상의 결과로 미루어 ALAD2 유전자형이 ALAD1 유전자형보다 납에 의한 체내대사에서 보호효과가 있을 것으로 추정된다. Objecthe: 6-Arninolevulinic acid dehydratase (ALAD) is a polymorphic enzyme that has two alleles, KAD1 (ALADl-1 as genotype) and M A D 2 (ALADI-2 or ALAD2-2 as genotype). AL.lhD genotype has been reported to mod@ the phmacokjnetics and toxicity of lead. The authors investigated the ionuence of ALAD genotype polymorphism on renal function in lead workers Methods: We studied 935 male lead workers and 87 male non-lead workers in the same industries. For cross-sectional renal indices, blood urea nitrogen (BUN), serum creatinine, semm uric acid and urine total protein were selected. Blood lead level was also measured an index of lead exposure. Information on weight, age, job duration, and smoking and drinking habits was collected. Results: Whereas the mean blood lead level of lead workers was 25.4f 10.9 PgldL, that of non-lead workers in the same premise was 10.1 f2.8 PgIdL, and the maence between the two groups was statistically signifcant. Whereas the prevalence of the variant allele, &AD2 in 935 lead workers was 10.6%, that in 87 non-lead workers was 4.4%. However there was no d~erence of prevalence between the two groups. The mean blood lead level of subjects with ALlhDl was 23.9f 11.4 Pg/dL, which was slightly lower than that of subjects with ALAD2 (25.8+ 10.7 pgldL). However, this differace was not statistically sigikficant. After adjustment for the covariates, the subjects with ALAD2 dele were 12.8% less &ely to have a median value or more of BUN than subject with ALAD1. Tihe adjusted odds ratio was 0.59 (95% confidence intervat 0.38-0.91). After adjustment for the covarjates, the subjects with ALAD2 allele were 9.3% less Wtely to have a median value or more of serum creatinine than subject with ALADl . The adjusted odds ratio was 0.64 (95% confidence interval; 0.41-0.98). Conclusions: From the above results, it was found that the variant allele, L A D 2 appeared to modify the association of lead and renal function, and that ALAD2 genotype may be supportive for the protective effect of lead.

65세 이상의 노령층에서 고지혈증의 위험요인

김용배,장원기,황보영,김화성,안규동,이병국,이용진,김세훈,김승수,김승훈,김영록,김웅빈,이성수 순천향의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

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납 노출 근로자들에서 ALAD 및 VDR의 다형질성이 조혈기능 지표에 미치는 영향

이성수,김남수,김진호,김용배,황보영,김화성,안규동,이병국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study was conducted with new workers who entered lead industries from 1992 to 2001 to evaluate the genetic susceptibility of ALAD (δ-aminolevulinic acid dehydratase) and VDR (vitamin D receptor) gene on health effect of lead exposure. Methods: Among the subjects of the database of lead industries at the Soonchunhyang University Institute of Industrial Medicine, only new workers were selected for this study. The total of eligible workers for this category was 3,540 workers, including non lead exposed workers of same lead industries. From stored blood in specimen bank of Soonchunhyang University, genotype of ALAD and VDR were measured using PCR method. Variables for this study were blood lead as an index of lead exposure, ZPP (zinc protoporphyrin in blood), urine ALA (δ-aminolevulinic acid), and hemoglobin as an index of hematopoietic effect of lead. Information on sex, job duration, and weight were collected for personal information. The data were analyzed using SAS (version 8.2) with descriptive analysis of t-test and multiple regression analysis. Results: Among 3,540 new employed study subjects during period of 1992-2001, 3,204 workers (90.5%) had ALAD genotype 1-1, while 336 workers (9.5%) had variant type of ALAD (1-2 or 2-2). For VDR genotype, 2,903 workers (89.7%) out of total tested 3,238 workers were belonged to type bb and 335 workers (10.5%) were type bB or BB. The distribution of genotype of ALAD and VDR were not different according to the job duration in male workers, but were different in female workers. The effect of ALAD and VDR genotype on blood lead were positively significant in the analysis of all cumulative data of new employed workers for 10 years. The effect of VDR genotype on blood lead were stronger than that of ALAD While the variant ALAD gene made decrease of mean ZPP and ALA in urine after controlling for blood lead and other covariate, the variant VDR gene made increased the mean ZPP and ALA in urine in all cumulative data analysis and cross sectional analysis by job duration. For hemoglobin, ALAD and VDR genotype did not affect the mean value. Conclusions: From the above our results, we found that ALAD and VDR genotype exerted significant effect in various way. We confirmed that the finding of a cross sectional study of protective effect of variant ALAD on the effect blood lead on blood ZPP in our retrospective study design. It was found that VDR did not exert protective effect for lead exposure as the variant ALAD did.

저강도 펄스형 초음파를 이용한 개의 슬개골 탈구 치료 효과

조성진,김남수,김화영,김선균,이성호,김병수 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

The effect of low-intensity ultrasonography on dislocation of the patella and fracture healing is controversial, and current management of that does not generally involve the use of ultrasound therapy. Nine patients with dislocation of the patella were treated with low-intensity ultrasonography(l5 mW/㎠, 1∼3.3 MHz) per 10-15 min/days, with an interval of 3 days during the last 3 weeks, at Family Animal Hospital(in Kunsan city). Ultrasound therapy may be beneficial to dislocation of the patella and healing. This finding is of considerable importance in that treatment with a low-intensity pulsed ultrasound signal may reduce healing time and could yield substantial cost savings and decreases in disability associated with delayed healing of dislocation of the patella.

수술실 손 소독제의 종류에 따른 균주 수의 변화

홍성윤,김정민,김소영,이수정,오은실,양서인,김화실,김남초 병원간호사회 2006 임상간호연구 Vol.11 No.2

Purpose: The present study purposed to compare the hand washing effect of 7.5% powidone-iodine, which is used in the operation room of C university hospital in Seoul, with that of Ethyl alcohol-CHG mixture recommended by the US Association of Operating Room Nurses (2002) and to adopt a more effective hand disinfectant. Method: In a quasi-experimental design, 48 medical staff who participated in operations during the period from November 2004 to February 2005 had hand washing using the two kinds of hand disinfectants: 7.5% povidone-iodine and the ethyl alcohol-CHG mixture. Their palms were swabbed and cultured just after hand washing and again after taking dff sterile gloves after the operation. The number of colonies from the two occasions were counted and compared. Result: The number of general bacillus increased significantly in the group using 7.5% povidone-iodine compared to that in the group using the ethyl alcohol-CHG mixture. The number of general bacillus increased signficantly in doctors compared to that in nurses. The factors affecting the increase of the number of general bacillus were disinfectants and medical personnel. The number of general bacillus was expected to increase 9.41 times with 7.5% povidone-iodine than with the ethyl alcohol-CHG mixture and 14.87 times in doctors than in nurses. Conclusion: This study shows that the ethyl alcogol-CHG mixture has a stronger hand disinfection effect than 7.5% povidone iodine. Thus we need to change the hand disinfectant used in operating rooms as soon as possible in order to minimize the infection of wounds resulting from operations.

제대혈 단핵구 냉동보존 기법에 따른 해동 후 세포 회복률 비교 : 급속 냉각 vs 통제속도 냉각 Dump Freezing vs Controlled Rate Freezing

오상철,김병수,윤수영,박경화,윤소영,김석진,서재홍,최철원,신상원,김열홍,김준석 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 저자 등은 조혈모세포를 -80℃ 냉동고에서 급속냉각법을 통하여 경제적으로 보존하는 방법의 냉동보존 효율이 통제속도냉각 후 액체질소보존법의 냉동보존 효율과 동등한 유효기간을 규명하고자 본 연구를 시행하게 되었다. 방법: 총 20 명의 산모에서 채집한 재대혈을 대상으로 하여 10% DMSO로 제대혈을 -80℃로 급속냉각한 경우(실험군)와 통제속도냉각장치로 냉각 후 액체질소냉동고에서 보존한 경우(대조군)에서의 해동 후 세포 회복률 및 기능의 회복 정도가 동등한 기간의 범위를 파악하기 위하여 3달 간격으로 총 18개월 동안 각 군 간의 해동 후 단핵세포회복률, CFU-GM 집락회복률, CD34+ 세포회복률 등을(평균)±(표준편차)로 표시하여 Paired t test를 통하여 비교, 분석하였다. 결과: 1) 생존 단핵세포 회복률: 대조군(통제속도냉각군)과 실험군(급속냉각군)을 비교하여 보았을 때, 모든 기간 동안 각 군 간에 유의한 차이를 발견할 수 없었다. 2) CFU-GM 집락 회복률: 대조군(통제속도냉각군)과 실험군(급속냉각군)을 비교하여 보았을 때 12개월 이후부터는 대조군이 실험군에 비하여 높은 경향의 회복률을 보였으나 양 군 간의 유의한 차이는 없었다. 3) CD34+세포 회복률: 대조군(통제속도 냉각군)과 실험군(급속 냉각군)을 비교하여 보았을 때 12개월 이후부터는 대조군이 실험군에 비하여 높은 경향의 회복률을 보였으나 양 군 간의 유의한 차이는 없었다. 결론: 제대혈에서 채집된 단핵구를 냉동보존 시 18개월까지 -80℃ 급속 냉각법으로 보존한 군과 통제속도 냉각 후 액체질소 보존 군 간에 단핵세포 회복률, CD34 양성 세포 회복률, CFU-GM 세포 회복률의 유의한 차이가 없었음을 관찰할 수 있었다. Background: This study was designed to compare the mononuclear cell recovery rate of cord blood after thawing and freezing according to cryopreservation methods: dump freezing vs controlled rate freezing. Methods: Twenty samples of cord blood were divided into dump freezing and controlled rate freezing groups. The viable mononuclear cell, CFU-GM and CD34+ cell recovery rates were evaluated at the time of post-thawing 1, 3, 6, 9, 12, 15 and 18 months after cryopreservation with 10% DMSO and compared between two groups. Results: The mean viable mononuclear cell recovery rates from cryopreservation 1 month to 18 months of dump freezing and controlled rate freezing were 81~88% and 82~89%, respectively. There was no statistical difference between two groups. The mean CFU-GM colony and CD34+cell recovery rates of dump freezing and controlled rate freezing (cryopreservation duration: 1 month to 18 months) were 66~76% vs 71~78% and 86~95% vs 86~94%, respectively. There was no statistical difference between the results of two groups. Conclusion: The -80℃ storage without rate-controlled freezing of cord blood was practically applicable with satisfactory cell recovery rates after thawing, which was comparable with those cryopreserved with controlled rated freezing and liquid nitrogen storage.

천명음이 동반된 기관 평활근종 1례

이혜승,김화종,강은정,김용현,김양기,이영목,김기업,어수택,황정화,김동원 순천향대학교 의학연구소 2006 Journal of Soonchunhyang Medical Science Vol.12 No.2

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관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.