산모 및 신생아 제대혈내의 B형간염 항원 및 항체분포에 관한 연구

金高昌,金舜謙,崔平和 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.3

The prevalence of hepatititis B surface antigen in Asia and West Pacific areas shows a high range from 0.1% to 15%, also in Korea around 10% in order to determine the prevalence of HBs Ag, Anti-HBs, Anti-HBc, HBeAg and Anti-HBe especially in the vertical transmission, above antigens and antibodies were detected by Radioimmunoassay in asymptomatic 361 pregnant women and their 361 newborn infants who were at Korea University Hospital during the period of 14 months from Jan. 1983 to Feb. 1984. The following results were obtained: 1. The majority was in birth weight of 3,001-3,5000㎎ and both sexes showed almost same ratio (51.2%:48.8%). 2. HBsAg was positive in 5.3% of pregnant women and 4.4% of newborn infants. 3. 16 newborn infants with HBsAg positive were born all to the HBsAg positive mothers. It shows very high percent (84.2%) of the vertical transmission. 4. There was no positive case of Anti-HBc, HBeAg and Anti-HBe in newborn infants, but only I case had Anti-HBs in infant which has been belived to be vertically transmitted in the end of the 2nd trimester. 5. 11 HBsAg positive infants were born to the both HBsAg and HBeAg positive mothers. The rest, 5 HBsAg positive infants, were born to HBsAg and Anti-HBeAg positive mothers which were thought of high percent of the vertical transmission (62.5%) in compared with other reports.

저산소증이 신생아 혈중갑상선 홀몬치에 미치는 영향에 관한 연구

김명자,최평화 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.3

Alteration in the thyroid metabolism of hypoxic, fasted, and chronically ill adults and older children have been described. Present study was carried out to evaluate the effects of hypoxia on thyroidal indices of term newborns and compared them to those of a control population. Blood was drown from the cord and then serially at 3, 24, and 48 hours after delivery in all patients. Twenty healthy term newborns (group I) increased their free thyroxine (FT₄) concentrations significantly after delivery from a mean ±SD baseline of 0.94±0.13 ng/dl in cord blood to a mean ± SD of 2.6±0.5 ng/dl, 48 h after delivery (p < 0.001), while their free triiodothyronine (FT₃) levels increased from a mean ± SD baseline level of 2.3±0.5 pg/ml in cord blood to a mean ± SD peak of 3.7±0.5 pg/ml 48 hours after delivery (p<0.001). Twentyone term newborns with transient low Apgar scores at birth (group Ⅱ) and sixteen term neonates born to mothers with toxemia or hypertension (groupⅢ) failed to increase their FT₄ and FT₃ concentrations above baseline during the first 48 hour of life. FT₄ and FT₄ values at 3, 24, and 48 hours were significantly higher in control group than in groupⅡ and group Ⅲ. Cord blood thyroid-stimulating hormone, FT₄ and FT₃ levels were not statistically different in the three groups. A rapid increase in thyroid-stimulating hormone values by 3 hour after delivery (to a mean ± SD of 16.6±3, 18.0 ± 1.2 and 17.0 ± 3.0 ul/ml in group, Ⅰ,Ⅱ, and Ⅲ, respectively), followed by a progressive thyroid-stimulating hormone decline to levels similar to baseline over the following 48 hours, was noted in all three groups of subjects.

토끼의 Aflatoxin B₁중독증에 대한 인삼엑기스의 효과 : 임상, 생리적 및 병리학적 변화 Clinical, Physiological and Pathological Changes

김차용,변유성,문평일,정덕화,김종수 慶尙大學校 1987 論文集 Vol.26 No.1

Chinchilla 계통 1~1.5㎏ 되는 토끼 30마리를 5군 으로 나누어 체중㎏당 제 1군은 대조군으로서 Cornoil 0.3ml 씩 제 2군은 aflatoxin B? 0.03mg과 인삼 엑기스 8mg을 제 5군은 aflatoxin B? 0.06mg과 인삼엑기스 8mg을 3주동안 카테타로 경구 투여하여 임상, 혈액학적 및 병리학적 변화를 관찰하였다. 1.사료소비량 감소, 침울, 쇠약, 혼수, 체중증가율감소등과 같은 임상증상은 대조군을 제외한 전 처리군에서 독을 투여후 3일째 부터 전처리기간을 통하여 나타났으나 aflatoxin B? 단독처리군에서 인삼엑기스 혼합처리군보다 더 현저하게 나타났다. 2. 혈청 효소와 AST, ALT는 독물투여후 7일부터 인삼엑기스 혼합투여군 보다 aflatoxin B? 단독 투여군에서 높게 증가하였다. 3.전처리군에서 적혈수용적, 헤모글로빈농도는 영향을 받지 않았고, 혈장단백질은ㅇ 독물투여후 14일이후 aflatoxin B?단독 처리군에서 현저하게 감소하였다. 4. Aflatoxin B?과 인삼엑기스 혼합 투여군 보다 aflatoxin B? 단독투여군에서 간장의 공포변성,담관증생, 핵농축, 붕괴, hepatic fibrosis와 임파절 및 비장에서 현저한 임파양 세포의 소실을 나타내었다. The present study has been carried out to investigate the effectsof ginseng extract on induced chronic aflatoxicosis in rabits. Groups of rabbits were treated orally with aflatoxin B? and mixture of ginseng extract for 21 days with a dosage of 0.03mg, 0.06mg of the toxin and 0.03mg or o.o6mg of the toxin mixture of 8mg of ginseng extract or 0.3ml corn oil s place to per kg body weight. The results obtained were is follows. 1. Sings o toxicosis included reduction of feed consumption emaciation, decrese of body weight and coma revealed in both groups treated with 0.03mg pr 0.06,mg of aflatoxin B?/Kg/B.W than groups 4.5 after days 3. 2. In groups 2, 3(aflatoxin B? 0.03, 0.06mg), Activity of asparate aminotransferase(AST) and alanine aminotransferase (ALT)were increased sigificantly in dats7, 14 and 21. 3. In all groups, Mean values for packed cell volume(PCV)and hemogobin were unremarkasble, but plasma total protein were decreased in groups 2,3than 4,5 groups on days 14 and 21. 4. The rabits treated with aflatoxin B?(2,3 groups)showed markedly degenerative chasnges ranging from swelling and decreaseed granularity to marked vacuolation, hepatic necrosis, fibrosis and bile duct, Proliferation and depletion of lymphocytes in lymphocytes in lymphonde, spleen than groups 4,5.

산재지정병원 이용 후 산재요양환자에 대한 만족도 조사

이화평,임형준,주영수,권영준,강희태,김정민,조성식,이태경,윤종완 대한산업의학회 2007 대한직업환경의학회지 Vol.19 No.3

母乳內 Amylase含量에 關한 硏究

吳東鮮,金舜謙,崔平和 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.1

It is well documented that human milk contains amylase but not in cow’s milk. Besides the level of amylase activity in saliva and in pancreatic juice is very low at birth, but increases during the first month of life. Present study was carried out to determined the amylase activity in breast milk from various stages of lactation and to compared with that of duodenal juice from infant. The effect of low pH on human milk amylase activity and contribution of human milk amylase activity to total amylase activity in duodenal juice were also studied. Total 201 samples of milk were obtained from 109 mothers during the March to July 1983 and results were as follows. 1) amylase activity was high in colostrums and somewhat lower in milk from day 14 to day 30 after delivery. In this period of lactation, breast milk contained higher amounts of amylase than duodenal juice from infants aged 1 to 6 months. 2) Low amylase activity was found in milk from 4 month or more after delivery. 3) A pH of 5.3 does not inactivate the amylase and there was considerable breast milk amylase activity in duodenal juice after a meal of breast milk. Breast milk amylase could thus contribute to the breast-fed infant’s ability to digest starch.

관상동맥 질환에서 CD14 유전자형에 따른 세균 및 Heat Shock Protein에 대한 반응의 차이

한주용,최수연,조현주,김화평,강현재,구본권,김남중,김효수,손대원,오병희,박영배,최윤식 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

Background : CD14 is the receptor for lipopolysaccharides and heat shock protein (HSP), which has been suggested being associated with increased risk of coronary artery disease (CAD). We investigated whether the response to infectious agents or HSP is different according to CD14 polymorphism in Koreans. Materials and Methods : Antibody titers to Helicobacter pylori, Chlamydia pneumoniae, and human HSP60 (hHSP60) were measured in 48 patients with stable CAD and in 41 healthy controls by ELISA. CD14 genotype was determined by PCR and high-sensitivity C-reactive protein (hs-CRP) was measured. Results : Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly associated with the presence of CAD. CD14 genotype distribution was 31 TT (35%), 43 CT (48%), and 15 CC (17%). To compare the response to the infectious organism and hHSP60, we divided study population into 3 groups; CAD patients with non-TT genotype (group I, n=30), CAD patients with TT genotype (group II, n=18), and normal controls (group III, n=41). Seropositivity to C. pneumoniae and H. pylori, and antibody titer to hHSP60 were not significantly different among 3 groups. Though hs-CRP level was significantly different among 3 groups, post-Hoc analysis showed that hs-CRP level was not significantly different between group I and group II (group I: 1.6[1.1-3.5] mg/L and group II: 0.35[0.1-2.0] mg/L). Conclusions : This study suggests that the inflammatory responses to infectious organisms and HSP do not differ according to the CD14 genotype in Koreans. 목적 : 만성적인 세균 감염이나 자가 면역 반응이 동맥경화와 연관되어 있다는 주장이 제기되어 왔다. CD14은 lipopolysaccharides (LPS)와 heat shock protein(HSP)의 수용체로 C(-260)→T 다형성이 관상동맥 질환의 위험과 관련되어 있다고 제안되어 왔다. 본 연구에서는 한국인에서 CD14 다형성에 따른 LPS와 HSP에 대한 반응성을 염증표지자를 측정하여 살펴보고자 하였다. 재료 및 방법 : 안정형 관상동맥 환자 48명과 정상 대조군 41명을 대상으로 Chlamydia pneumoniae, Helicobacter pylori 항체 및 인체 HSP60 (hHSP60) 항체 역가를 enzyme-linked immunosorbent assay (ELISA) 방법으로 측정하였다. CD14 유전자형은 중합효소연쇄반응을 이용하여 결정하였고 hs-CRP를 측정하였다. 결과 : C. pneumonias 및 H. pylori 항체 양성 여부 및 항체 역가, 그리고 hHSP60에 대한 항체 역가는 안정형 관상동맥 질환의 유무와 유의한 상관관계는 없었다. CD14 유전자형의 분포는 TT 30명(39%), CT 31명(40%), 그리고 CC 16명(21%)이었다. 관상동맥 질환 군에서 TT 유전자형은 38%, 대조군에서는 32%로 TT 유전자형과 관상동맥 질환의 유무와는 유의한 상관관계가 발견되지 않았다(P>0.05). CD14 유전자형에 따라 C. pneumonias와 H.pylori 감염 및 hHSP60에 따른 반응에 차이가 있는지 여 부를 알아보기 위해, 관상동맥 질환 군을 다시 TT 유전자형 군과 non-TT 유전자형 군으로 나누어, 전체적으로 세군에서 항체 양성률과 역가를 비교하였다(I군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, II군: CD14 TT 유전자형을 가진 안정형 관상동맥 환자, III군: 정상 대조군). 세 군에서 C. pneumonias와 H. pylori 항체 양성률 및 hHSP60 항체 역가에는 유의한 차이가 없었다. 세 군사이에 hs-CRP 값에 유의한 차이가 있었지만, post-Hoc분석에서 II군의 hs-CRP 값이 I군에 비해 유의하게 높지는 않았다(0.35 [0.1-2.0] mg/L in group II vs. 1.6 [1.1-3.5]mg/L in group I, P>0.05). 결론 : 본 연구는 한국인에서 CD14 유전자형에 따라 LPS나 HSP에 대한 반응이 유의하게 다르지 않음을 시사한다.

FET형 반도체 마이크로센서 개발(Ⅱ)

손병기,이흥락,박이순,조진호,이성필,최평,서화일,고성택,박재윤,서장수,김창수 경북대학교 센서기술연구소 1994 연차보고서 Vol.1994 No.-

현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte : H^+, K^+, Ca^2+, Na^+)분석 시스템, 용존가스(O_2, CO_2)분석 시스템의 개발을 중점적으로 추진하며, FET형 압력센서, 습도센서, 방사형 온도센서 등의 개별 FET형 센서에 관한 연구도 병행하고자 한다. Recently various kinds of sensors have been developed, being applicated to their own purpose. There are lots of difficulties to apply them to measurements in which the real-time monitoring is required without disturbing the surrounding environment. FET type semiconductor sensors, fabricated by the semiconductor integration technologies, have many advantages for their miniaturization, standardization, mass-production and in vivo/in situ monitoring. They also hold a very proper configuration for multi-functional sensors or integrated smart sensors, and wide availability by forming various kinds of physical or chemical sensing materials onto their sensing gates.

FET형 반도체 마이크로센서 개발(Ⅲ)

손병기,이흥락,박이순,조진호,이성필,최평,서화일,박재윤,송경은,김창수 경북대학교 센서기술연구소 1995 연차보고서 Vol.1995 No.-

현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte : H^+, K^+, Ca^2+, Na^+)센서소자 및 분석 시스템, 용존가스(O_2, CO_2)센서의 개발을 중점적으로 추진하였으며, FET형 압력센서, 습도센서 둥의 개별 FET형 센서에 관한 기초연구도 병행하였다. The conventional sensors have many problems such as high cost, large dimension, long analysis time and troublesome handling to apply to the fields of medical diagnosis, monitoring of chemical process and environmental monitoring/control. The main objects of this research are to develope a new FET type electrolyte(H^+, K^+, Ca^2+, Na^+)sensors, analysis system, and dissoved gas(O_2, CO_2)senors that can overcome the problems of the conventional sensors, and parallel basic researches on FET. type sensors such as pressure and humidity are also in progress.

T-Type Calcium Channels Are Required to Maintain Viability of Neural Progenitor Cells

Kim, Ji-Woon,Oh, Hyun Ah,Lee, Sung Hoon,Kim, Ki Chan,Eun, Pyung Hwa,Ko, Mee Jung,Gonzales, Edson Luck T.,Seung, Hana,Kim, Seonmin,Bahn, Geon Ho,Shin, Chan Young The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.5

T-type calcium channels are low voltage-activated calcium channels that evoke small and transient calcium currents. Recently, T-type calcium channels have been implicated in neurodevelopmental disorders such as autism spectrum disorder and neural tube defects. However, their function during embryonic development is largely unknown. Here, we investigated the function and expression of T-type calcium channels in embryonic neural progenitor cells (NPCs). First, we compared the expression of T-type calcium channel subtypes (CaV3.1, 3.2, and 3.3) in NPCs and differentiated neural cells (neurons and astrocytes). We detected all subtypes in neurons but not in astrocytes. In NPCs, CaV3.1 was the dominant subtype, whereas CaV3.2 was weakly expressed, and CaV3.3 was not detected. Next, we determined CaV3.1 expression levels in the cortex during early brain development. Expression levels of CaV3.1 in the embryonic period were transiently decreased during the perinatal period and increased at postnatal day 11. We then pharmacologically blocked T-type calcium channels to determine the effects in neuronal cells. The blockade of T-type calcium channels reduced cell viability, and induced apoptotic cell death in NPCs but not in differentiated astrocytes. Furthermore, blocking T-type calcium channels rapidly reduced AKT-phosphorylation (Ser473) and $GSK3{\beta}$-phosphorylation (Ser9). Our results suggest that T-type calcium channels play essential roles in maintaining NPC viability, and T-type calcium channel blockers are toxic to embryonic neural cells, and may potentially be responsible for neurodevelopmental disorders.

성인에서의 담관 낭종과 췌담관 합류 이상

김명환,김영환,김효정,이성구,서동완,최호순,이승규,유병무,민영일,고문수,민병철,이미화,임미경,김표년 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.1

Background/Aims: Choledochal cyst is a congenital dilatation of extraor intrahepatic bile duct . Recently, anomalous union of pancreaticobiliary duct(AUPBD) is supposed to be one of the major causes of choledochal cyst. The aims of this study are to assess the association ratio of AUPBD in choledochal cyst and whether the AUPBD in choledochal cyst have a clinical significance. Methods: The study subjects were 35 adult patients who were diagnosed by ERCP in Asan Medical Center from August, 1990 to July, 1995. We have classified the type of choledochal cyst and AUPBD according to Todani and Kimura, respectively. Result: The number of choledochal cyst was 14 cases(40%) in type I, I case(3%) in type II, 6 cases(17%) in type Ⅲ, 11 cases(31%) in type IVA. Three cases were unclassified. Among the 35 cases, 22 cases(63%) of choledochal cyst were associated with AUPBD. AUPBD was only seen in type I, IVA and unclassified form of choledochal cyst. The number of patients with type 1 AUPBD was 10(45%) and type 2 was 12(55%) by Kimura's classification. 'Che mean age was younger in patients with choledochal cyst and AUPBD(36.2 16 years) than in choledochal cyst aione(49.3 19 years). Acute inflammation and malignancy of biliary tree 8z pancreas were more commonly seen in patients with choledochal cyst and AUPBD than choledochal cyst alone. Conclusion: In patients with choledochal cyst, the association of AUPBD was not uncommon and they had more grave clinical course. AUPBD may be a contributing factor to develop mali~ancy in patiehts with choledochal cyst.