흰쥐 Kupffer 세포에 대한 Propofol의 작용

박세훈,지대림,성언기,김희선,송인환,박희영,김준수,이덕희,박대팔 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

Background: Propofol has an antioxidant capacity and can be used for ischemia-reperfusion injury of the liver. However, the effects of propofol on the Kupffer cells have not been establisked. Methods: Kupffer cells were isolated and cultured from male Sprague-Dawley rats. The effects of propofol on the Kupffer cells were evaluated by a phagocytosis assay, TNF-α gene expression, and superoxide anion release after administering propofol in different concentrations on the cultured Kuprrer cells. Results: The latex bead phagocytosis by the Kupffer cells was suppressed when the Kupffer cells were exposed to propofol irrespective of concentrations. Higher propofol concentrations decreased the loss of Kupffer cells after latex bead phagocytosis. Propofol induced TNF-α mRNA expression in the Kupffer cells, but the mRNA expression level after 50㎍/ml of propofol decreased. The pattern of TNF-α mRNA expression induced by propofol was different to that induced by LPS: TNF-α mRNA was expressed continuously in the propofol-treated cells until 16 hours after exposure to propofol, whereas the level of TNF-α mRNA expression induced by LPS was evident after 2 hours and was not found thereafter. TNF-α production after propofol treatment was not higher than that of the control. Formazan presipitation did not show any qualitative differences between cells untreated or treated with propofol concentrations of 0.5, 5.0, and 50 ㎍/ml. Conclusions: These results showed that propofol might inhibit Kupffer cells. This suggests that propofol and be used for patients with ischemia-reperfusion injury of the liver. (Korean J Anesthesiol 2002; 43: 475~484)

[논문]Operational Characteristics of CO2 Laser Adopted by Superposing-Discharge

Hee-Je Kim,Hyun-Ju Chung,Byoung-Dae Min,Jong-Han Joung,Sung- lun Park 釜山大學校生産技術硏究所 2003 生産技術硏究所論文集 Vol.62 No.-

오늘날 현저한 CO2 레이저의 응용 추세에 발맞추어 보다 다양한 형태의 출력 제어 기술이 대두되고 있다. 본연구에서는multi-Pulse Superposing-Discharge 기술을 이용하여 다양한형태의 CO2 레이저의 펄스를 성형 하였다. 펄스 성형을 위한 방법으로 PIC one-chip microprocessor를 이용하여 삼중 PFN 모률의 SCR을 각각 고유의 지연시간을 가지도록 제어하였다. 실험에 사용된 삼중 PFN 모률의 전원 회로는 각각 캐패시터 인덕터,SCR,고압 펄스 트랜스 그리고 고압 트랜스 2차측에 브릿지 정류기로 구성 하였다. 고압 펼스 트랜스의 1차측을 제어하므로서 PFN 모률의 동작을 저 전압에서 수행 할 수 있다. 트랜스의 2차측에 연결된 전파 정류기는 펄스 에너지를 부하에 지속적으로 전달한다. 본 연구에서는 삼중 PFN 모률의 각각의 SCR을 다른 시간 가격을 두고 트리거 하므로서 다양한 Long pulse shaping을 실현하였다. 또한 약 250-1000μs 의 주기를 가지는 다양한 펄스형 레이저 빔을 얻었다.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

서초동(서울시 서초구)의 거미상

김주필(Joo Pil Kim),정혜리(Hye ri Jeong),김대희(Dae Hee Kim),박동우(Dong Woo Park),서재웅(Jae Woong Seo),이현우(Hyun woo Lee) 한국거미연구소 2017 한국거미 Vol.33 No.1

2016년 3월 31일부터 7월 31일까지 서울특별시 서초구 서초동에 위치한 서울고등학교 교정 일대를 서울고등학교 환경생물부 부원들이 채집 조사한 결과 20과 43속 53종의 거미가 조사 연구되어 이에 보고하는 바이다. From March 31st to July 31st in 2016, members of an environmental biology club at Seoul High School located in Seocho-gu, Seoul, Dae-Hee Kim, Dong-Woo Park, Jae-Woong Seo and Hyun-woo Lee, collected 20 families 43 genera 53 species of spiders and report the result of the study here.

실업계 고등학생의 직업기초능력 향상을 위한 문제중심학습(PBL)의 효과

박동열,이성덕,김종오,조경희,강경심,박윤희,김대영 한국농업교육학회 2006 농업교육과 인적자원개발 Vol.38 No.4

21세기 지식기반사회는 전문지식뿐만 아니라 효율적인 직무 수행에 필요한 의사소통능력, 문제해결능력, 자기관리능력 및 직업의식 등의 직업기초능력이 강조되고 있다. 직업기초능력은 생의 어느 한 시기에 갑자기 이루어지거나 완성되는 l회적인 것이 아니라 개인의 생애단계에 따라 변화하고 발달하는 것이며 취업자뿐만 아니라 진학자에게도 잠재적으로 매우 중요한 요소이므로 실업계 고등학생의 직업기초능력 향상은 중요한 현안이라 할 수 있다. 이 연구는 여러 교수학습유형 중 실업계 고등학생의 직업기초능력 향상에 타당하리라 사료되는 문제 중심학습 모형(Problem-based learning model)을 선정, 이에 기초한 전문교과 수업을 실시하여 실업계 고등학생의 직업기초능력 향상에 기여할 수 있는지 실증적으로 분석하고자 하였다. 연구 목적 달성을 위해 「대학생의 직업기초능력 유형 검사도구(박동열, 2005)」를 실업계 고등학교 전문가 협의회를 통해 수정ㆍ보완, 이를 활용하여 실업계 고등학생의 직업기초능력을 파악하고, 문제중심학습 모형에 따른 실제 전문교과 수업을 통한 실업계 고등학생의 직업기초능력 향상 정도를 알아보기 위하여 농업계열, 공업계열, 상업계열, 가사ㆍ실업계열 4명의 현직 교사가 문제중심학습 수업 매뉴얼에 따라 실제 수업에 적용 하고 그 결과를 분석하였다. 연구 결과 문제중심학습 모형이 실업계 고등학생의 수업만족도에 긍정적인 영향을 끼쳤으며, 문제중심학습 모형에 따른 전문교과 수업을 통해 실업계 고등학생의 모든 영역의 직업기초능력(문제해결 및 발표 능력, 기술활용능력, 자원활용능력, 수리능력, 조직이해능력, 대인관계능력, 자기관리능력)이 향상되었음을 실증적으로 알 수 있었다. The purpose of this research was to analyze the fitness of problem-based learning(PBL) for improving the core competencies of vocational high school students. This study examined the core competencies of vocational high school students by using the advised BARS test for vocational high school. And in order to examine the effectiveness of the courses based on PBL, 4 teachers had taught the designed courses by PBL method during the regular class in vocational high school : agricultural; technical; commercial, home economic high schools. The major findings in this study were as follows: 1) The courses based on PBL had an positive effect on the class satisfaction of vocational High school students. 2) We found out that the courses based on PBL have a positive effect on improving vocational key competencies of Vocational High school students.

선천성상부요로기형에서 발생한 요석에 대한 체외충격파쇄석술

박희수,박경민,정용호,김철성,장대수 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

Purpose: We sought to determine the efficacy of extracorporeal shock wave lithotripsy(ESWL) in the treatment of urolithiasis with congenital upper urinary tract anomalies. Materials and Methods: Using the Northgate SD-3 lithotriptor, 43 patients with calculi with upper urinary tract anomalies were treated with extracorporeal shock wave lithotripsy(ESWL) at Chosun University Hospital from 1996 to 1998. The upper urinary tract anomalies were duplicated ureter in 12 cases, bifid pelvis in 10, malrotated kidney in 4, ureterocele in 3, UPJ obstruction in 3, caliceal diverticulum in 3, horseshoe kidney in 3, pelvic kidney in 2, medullary sponge kidney(MSK) in 2 and polycystic kidney in 1. The age range of the patients was from 22 to 70 years of age(mean of 49.6 years). There were 23 men and 20 women. The mean size of the stone was 10.1x8.6±4.9x4.3mm, General or regional anesthesia was not required. Results: The average frequency was 2.3 sessions and the average number of shock wave was 2258±1404 shock. The 3-month stone free rate was 90.7%, Complications of stone removal were petechia in 43 cases, gross hematuria in 36, severe flank pain in 9 and urinary infection in 2. All complications were treated successfully with conservative management. Conclusion: We conclude that ESWL monotherapy is a safe and successful modality as a noninvasive management of urolithiasis in patients with upper urinary tract anomalies.

CT 유도하의 경피적 폐생검 : 임상적 의의 및 합병증에 관한 연구 A Study of Clinical Significance and Complication

박재성,백상현,차장규,홍현숙,김대호,박성진,조준희,임한혁,이혜경 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose : To determine the diagnostic accuracy and predicting factor of pneumothorax on CT-guided percutaneous transthoracic needle biopsy. Materials and Methods : Using automated biopsy devices, 107 CT-guided percutaneous needle biopsies of pulmonary lesions were performed. Final diagnosis was made with operation or other methods. We retrospectively analyzed the frequency of complications and diagnostic yields of 107 biopsies. The clinical features, treatment, and courses of complications were reviewed, and risk factors of pneumothorax such as depth and size of lesion, CT emphysema score of whole lung and pathway of needle, and the result of pulmonary function test were analyzed. Result : 96 biopsies(89.7%) yielded sufficient tissue for pathologic evaluation. For cases of malignant and benign disease, sensitivity was 91.5% and 87.5% respectively; the corresponding figures for diagnostic accuracy were 88.1% and 77.1%. So, sensitivity and diagnostic accuracy of percutaneous transthoracic needle biopsy was 89.7% and 83.2%. Eighteen(16.8%) of 107 patients developed pneumothorax and 3 of these required application of chest tube or percutaneous catheter drainage. In the group with pneumonthorax, the lesion was significantly smaller(p=0.029) and higher CT emphysema score of pathway of needle(p=0.032). But there were no significant difference between two groups(with or without pneumothorax) for depth of lesion, pulmonary function test(% predictive value of FEV1 and FEV1/FVC) and CT emphysema score of whole lung. Conclusion : CT-guided transthoracic needle biopsy of the pulmonary lesions is safe and easy method of obtaining specimens of good quality and quantity. It may be an useful procedure in the diagnosis of localized pulmonary lesions. For the prediction of pneumothorax, size of lesion and CT emphysema score of pathway of a needle are significant risk factors.

Rett syndrome 환자의 제증상에 관한 증례보고 : A CASE REPORT

박성진,이광희,김대업 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

레트 증후군(Rett syndrome)은 1983년 Andreas Rett에 의해 처음으로 보고되었다. 여성에서 주로 호발하여 다양한 민족에서 발견되는 신경장애 중의 하나이다. 레트 증후군의 가장 특징적인 장애의 양상으로 실행증을 들 수 있으며 성장에 따라 집중해서 보는 듯한 눈을 보이기도 한다. 대부분의 레트 증후군은 자폐, 뇌성마비, 규정되지 않은 발달지체로 오진된다. 레트 증후군의 원인은 유전적 요소가 있는 것으로 보이나 아직가지 확실히 밝혀지지 않았다. 본 증례에서 레트 증후군 환아는 과개교합으로 인한 상악 구개부의 손상을 주소로 내원하였다. 상악 구개부의 손상을 감소시키기 위해 교합판을 제작하여 만족할 만한 결과를 얻었기에 보고하는 바이다. Rett syndrome is a neurological disorder seen almost exclusively in females, and found in a variety of racial and ethnic groups worldwide. First described by Dr. Andreas Rett in 1983. The specific features of the Rett syndrome is apraxia. Most of the Rett syndrome has been diagnosed erroneously to autism, cerebral palsy, and unknown developmental disorders. The etiology of the Rett syndrome is not figured out exactly but it seem to have relation with genetic factors. In this case the patient with Rett syndrome had a chief complaint of the injury of palate due to deep bite. We report this case for the satisfactory result using the bite plane to decrease the injury of the palate due to deep bite.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.