유제놀에 의한 즉시형 알레르기 반응의 억제

김상현,신태용,김형룡,이영미,이은희,신보경,김윤철,안년형,김형민 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The current study evaluates the capacity of eugenol to regulate immediate allergic reaction by control of histamine release. Administrations of eugenol (1 M/kg. i.p.) at 60 min before and 5.10 min after the compound 48/80 treatment (8 mg/kg.i.p.) were shown the mortality rates as 0.44.4. and 77.8%. respectively. A 60 min before administered group revealed a singificant inhibition of serum his-tamine release compared with those of 5 and 10 min after the compound 48/80 injection. Eugenol (6-48mM) was also showed a dose-dependent activity on the compound 48/80-induced histamine release from the highly purified population of Alcian Blue-positive peritoneal mast cells. These results indicate that in vitro treatment with exogenous eugenol inhibited the active response of mast cell populations and modulated its characteristics.

박의 RAPD 분석을 위한 PCR 최적 조건 연구

김보경,김성만,김용철,이충열,박현철,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

This study was conducted to identify the best combinations of factors(template DNA concentration, MgCl2 concentration, and amount of taq polymerase) for the optimization of PCR in gourd. 3×3×3 factorial experiment for template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The most clear and reproducible bands were appeared in 40ng(1unit taq polymerase and 2.5mM MgCl2, and lunit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 7.0mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). The best MgCl2 concentration was 4.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 0.5unit taq polymerase, and 60ng template DNA and lunit taq polymerase), and 7.0mM(40ng template DNA and 0.5unit taq polymerase DNA). The combinations which showed the best bands from the amount of taq polymerase were 0.5unit(60ng template DNA and 4.5mM MgCl2) and lunit(40ng template DNA and 2.5mM MgCl2, 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 1 condition(40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase) was the best combination for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃ and 86℃, 35℃, 64℃.

배추의 RAPD 적정 조건 구명

김보경,최인수 밀양대학교 농업기술개발연구소 2000 農業技術開發硏究所報 Vol.4 No.1

It is very important to identify the optimized PCR condition for analyses using PCR. The objective of this study was to optimize PCR condition for RAPD analysis in chinese cabbage. 3×3×3 factorial experiment far template DNA concentration, MgCl2 concentration, and amount of taq polymerase was conducted. Another factorial experiment for denature, annealing, extension temperature on the optimal PCR condition was also conducted. The best template DNA concentration was 40ng(1unit taq polymerase and 2.5mM MgCl2, and 1unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 7.0mM MgCl2), 60ng(0.5unit taq polymerase and 2.5mM MgCl2, 0.5unit taq polymerase and 4.5mM MgCl2, 1unit taq polymerase and 2.5mM MgCl2, 1unit taq polymerase and 4.5mM MgCl2, and 1unit taq polymerase and 7.0mM MgCl2). Clear bands were observed from 4.5mM(40ng template DNA and 1unit taq polymerase, 60ng template DNA and 1unit taq polymerase and 60ng template DNA and lunit taq polymerase), and 7.0mM(60ng template DNA and 1unit taq polymerase). The best amount of taq polymerase was 0.5unit(60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2), and 1unit(40ng template DNA and 2.5mM MgCl2. 40ng template DNA and 4.5mM MgCl2, 40ng template DNA and 7.0mM MgCl2, 60ng template DNA and 2.5mM MgCl2, 60ng template DNA and 4.5mM MgCl2, 60ng template DNA and 7.0mM MgCl2). When we consider results from template DNA concentration, MgCl2 concentration, and amount of taq polymerase, 3 conditions(40ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase; 60ng of template DNA, 4.5mM MgCl2 and 1unit taq polymerase; 60ng of template DNA, 7.0mM MgCl2 and 1unit taq polymerase) were best combinations for the optimal PCR condition. Reaction temperatures for the optimal PCR condition were 84℃, 32℃, 62℃ and 86℃, 35℃, 64℃.

서울 시내 일부 대학생의 성지식과 성태도에 관한 연구

김보배,노은경,박서영,박영,오승희,전영란,차현진,윤희상,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

This study surveyed and analyzed comparatively college students-knowledge' attitude, was to cultivate the right knowledge and attitude on sex, and serve the foundational data for the suitable sex education. The purpose of the study was to identify and examine: 1) students; sexual knowledge and sexual attitude; 2) the differences between variables of gender, age, and self-perceived sexual knowledge in relation to sexual knowledge and sexual attitude; and 3) the relationship between sexual knowledge and sexual attitude. For this study the questionaires were given to 300 students from five different universities located seoul areas. the questionaires were collected Jenuary 8th to 15th in 2004. Two hundred and nigty one samples were analyzed for the study. The adapted SKAT(saxual knowledge & attitude test; Lief & Reed, 1986) was used to determine sexual knowledge and the students' sexual attitude. Collected data were analyzed statistically by means of percentage, t-test, ANOVA test, and pearson correlation analysis with SPSS 12.0 package program. The main results of this analysis are as follows: Participants consisted of male(48.1%) and female(51.9%) which were mainly between the ages of 21.25(71.8%) with an age range of 19 to 30. Knowledge of sexual disease was scored highest of 92.4%, and konwledge of the knowledge of the male and female's anatomy was the lowest at 60.9%. The respondents' level of sexual attitude was tolerant as on average 3.66(on the basis of 5) Relating to the items, the respondents' score of the sexual curiosity was 4.16, sexual behavior 3.85, self-consolation 3.81, aborthon 3.23, virgine 3.19, extramarital experiences 3.17. There were no significant differences in sexual knowledge between male and females, between younger and older students, residence, sexual experience, and how students mainly obtained their sexual knowledge. But a significant difference was also found religion in their sexual attitude(p<.005). In addition, results showed the relationship between sexual knowledge and sexual attitude(P=0.332).

대학생의 성별, 학년별, 전공별 스트레스에 관한 연구

김보혜,안윤정,정민영,차지영,최경원,김지현,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

The purpose of this study was to investigate the level and effect of stress on physical and mental health. Based on that result, we offer the basic research to lessen students' stress and make a program to promote health condition effectively. Subjects were 526 university students. (male: 232(44.1%) female: 294(55.9%). composed of 7 majors: human studies&social science, management, Science&technology, education. law, medicine, entertainment. freshman 108(20.5%), sophomore 140(26.6%) junior 148(28.1%), senior 130(24.7%)) Subjects were filled with 127 questionnaires. T test and ANOVA were used to evaluate each stress levels. The .05 level of significance was the critical for this research. Also used the correlation to know the relation between stress levels and effects. The result of the study were as follows: 1. According to gender, male students got 2.37, female students got 2.46 on the stress level(p=.05). And also the belonging items showed female students got higher stresses than male students. 2. According to grade, the average score of stress level was 2.42. sophomores got the highest stress and freshmen got the lowest stress level. But that was not meaningful difference. freshman and sophomore got higher stress on studying. and except the freshman, all grades got severe stress on employment. 3. According to major, there were differences on university life and surroundings. On university life, the students who majored management got highest stress and the students who majored Education got the least stress. And on surroundings, the students who majored Law got highest stress. 4. According to gender, female students suffered from more stress effects than male students.(male 2.3725 female 2.4657 t:-1.968 p:.050) 5. According to grade, the average score of stress was 2.2285. sophomore got 2.3042 and junior got 2.2148 on stress effects. 6. According to major, The students who major in law got more stress effects caused by family than any other students. The students who major in entertainment and gymnastics got more stress effects than any other students in surroundings. 7. There were significant correlations between the stress level and effects(r.=847, p=.000).

엔진구동형 NiTi 파일의 근관성형능력 비교

김보혜,최경규,박상혁,최기운 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.2

The purpose of this study was to compare the root canal shaping ability of 4 rotary NiTi instruments in simulated root canals. For the preparation of thirty two curved root canals, Mtwo instruments using “single length”technique, and Profile, ProTaper Universal, and K3 using crown-down technique (N = 8) were used. All canal samples were prepared by reaching an apical canal size of #30. Pre- and post-instrumentation digital images were recorded and an assessment of canal shape was determined using a computer image analysis program SigmaScan Pro (Systat Software Inc., San Jose, CA, USA). The changes of the dimension of inner walls of canals, (2) the changes of the dimension of outer walls of canals, and (3) the centering ratio were measured at 7 measuring points, and then data were statistically analyzed using one-way ANOVA and Duncan’s test. The results were as below; 1. The root canal shaping ability of Profile was significantly faster than that of other rotary NiTi instruments (p < 0.05). 2. The deformation and fracture of all instruments used for this study were not experienced. 3. In the degree of changes of the dimension of inner walls of canals, Profile demonstrated the lowest changes of the dimension of inner walls of canals except at the measuring points of the 1 and 2 mm (p < 0.05). However, the ProTaper Universal showed the highest changes of the dimension of inner walls of canals at all measuring points (p < 0.05). 4. In the degree of changes of the dimension of outer walls of canals, Mtwo demonstrated the lowest changse of the dimension of outer walls of canals except at the measuring point of the 1 mm (p < 0.05). However, Profile exhibited the highest changes of the dimension of outer walls of canals at the measuring points of 3 and 4 mm and ProTaper Universal and K3 showed the largest changes of the dimension of outer walls of canals at the measuring points of 1, 2, 6, and 7 mm (p < 0.05). 5. In degree of centering ratio, Profile demonstrated the least centering ratio comparing with the centering ratio shown by other NiTi instruments at the measuring points of 1, 4, 5, and 6 mm. Results suggest that in the coronal part of canal preparation, active cutting files such as ProTaper Universal may efficiently flare the canal orifice and form a better taper, and in the apical part of the canal, files which have a better centering ability such as Profile may maintain the original canal curvature and reduce the shaping time. 이 연구의 목적은 4가지 엔진구동형 NiTi 기구의 근관성형력을 비교하는 것이다. 32개의 투명레진모형을 사용하여 파일의 단면도가 S자 형태이고“single length”방법으로 근관성형을 시행하는 Mtwo와 crown-down 방법으로 근관성형을 하는 Profile, ProTaper Universal 및 K3로 근관성형을 하였다 (n = 8). 모든 근관은 한사람의 술자가 근단공 크기가 #30이 될때까지 성형하였다. 기구 사용 전과 후 근관 모양을 디지털 영상을 기록하였고, 컴퓨터 영상 분석 프로그램(Expression Scanner와 Adobe Photoshop CS)으로 평가하였다. 다음과 같은 결론을 얻었다. 근첨으로부터 1, 2, 3, 4, 5, 6, 7 mm 떨어진 부위의 내측과 외측으로 삭제된 레진 양을 측정하였고, 자료는 SPSS법으로 비교 분석하였다. 1. 근관형성 시간은 Mtwo가 84.50초로 가장 짧았으며, 총 작업시간은 K3가 269.37초로 가장 길었다(p < 0.05). 2. 기구의 변형과 파절은 모든 구동형 NiTi 파일에서 발생하지 않았다. 3. 근관 내측 폭경의 변화는 Profile이 1, 2 mm 부위를 제외한 다른 부위에서 가장 적었으며, ProTaper Universal은 모든 부위에서 가장 많았다(p < 0.05). 4. 근관 외측 폭경의 변화는 Mtwo이 1 mm 부위를 제외한 모든 부위에서 가장 적었으며, Profile은 3, 4 mm 부위에서 가장 많았다(p < 0.05). ProTaper Universal와 K3는 1, 2, 6, 7 mm 부위에서 가장 많았다(p < 0.05). 5. 근관중심변위율(centering ratio)은 Profile이 1, 4, 5, 6 mm 부위에서 가장 적었다(p < 0.05). 이상의 결과, 구동성 NiTi 파일을 이용한 만곡 근관의 근관형성은 치경부는 ProTaper와 같은 삭제력이 높은 파일을 사용하는 것이 근관 입구를 효과적으로 넓히고 더 나은 taper를 형성하며, 근단부는 Profile과 같은 근관 중심 변위가 적은 파일을 사용하는 것이 근관형성 시간을 단축하고 근단부의 근관 변형(transportation)을 줄일 수 있다고 판단된다.

신경회로망을 바탕으로 한 확률적 최적화 실험계획법

김보경,이석훈 충남대학교 1991 忠南科學硏究誌 Vol.18 No.1

This paper studies the schemes to apply the Simulated Annealing algorithm that is used as an optimization stchnique for neural Network as a technique for finding the optimal design of experiments and compares this implemented results with those of Branch and Bound algorithm which completely gurarantees the optimality in order to evaluate their performance. In this comparison, D-optimality is used as a criterion for optimization.

대학생의 수면양상과 수면장애요인에 관한 연구

김유진,박미경,박이랑,이보람,이혜림,전선미,양난영,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

The results of this Study are as follows:33.6% of all participants have insomnia; 22.5% of those who have insomnia are DIS(difficulty in initiating sleep), 17.3% are DMS(difficulty returning to sleep once awakened) and 7.0% awakened too early. 3 4.8% experience sleepiness during daytime. Type 1, experiencing insomnia and sleepi ness during daytime together, is 12.0%, Type 2, with insomnia only, is 21.6%, Type 3, with sleepiness during daytime only, is 22.8% and 43.5% experience no sleeping disturbances. After studying only those with 3 types of sleeping disturbances, it is found that the most common cause of such disturbance is stress 88.4%, anxiety 56.0%, no apparent reason 33.8%, anxiety/fear/terror 29.3%, hurry 23.6%, alcohol/caffeine 16.9%, bedroom tem perature 11.1%, urination during nighttime and persons living together 10.7%, noise from inside 8.9%, illumination 8.0%, and pain/itch 5.8%. The one group revealed significant differences in residential environment(p=0.003). Sex, age, education level, medicine, monthly earning revealed no meaningful differences. Of sleeping behavior, mean duration of sleep latency(p=0.000), whether or not feeling freshness(p=0.000), whether taking enough sleep(p=0.029), whether taking regular sleep(p=0.005) showed significant differences depending on whether or not having insomnia, and mean duration of sleep time, time to sleep, time of rising, whether taking naps did not reveal significant differences. Of sleep behavior, time to sleep(p=0.000), whether taking naps(p=0.000), indicated significant differences. Of sleeping behavior, mean duration of sleep latency(p=0.000), whether or not feeling freshness(p=0.000), and whether taking enough sleep(p=0.000), time of going to bed (p=0.002), whether or not taking nap(p=0.000), whether or not taking regular sleep(p=0.010) indicated significant differences among the sleeping disturbance types.

유색미에 관한 연구 : Ⅲ. 유색미 유망계통에 대한 주요 생육 특성

김광수,최윤표,김선택,최현구,정종태,김보경,유지홍,이희봉 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.2

국내외 유색미 수집종을 돌연변이원에 의해 유기된 우수 계통과 수집종간 상호교배에 의해 선발된 CNU 20계통을 파종하여 얻어진 주요 작물학적 특성을 살펴보면 다음과 같다. 1. 간장은 유색 메벼인 CNU126 계통이 대조품종인 동진벼와 같이 가장 컸고 메성인 CNU 128계통에서 가장 작았다. 2. 주당 분얼수는 CNU3, CNU50. CNU56, CNU112 계통에서 가장 많았고 대조품종과 CNU 128 계통에서 10개 미만으로 가장 적게 나타났다. 3. 수장은 육성 계통 중 CNU88 계통에서 가장 길었으나 대조구보다 짧고, 육성계통 중에서는 CNU 126 계통이 가장 짧았다. 4. 주당 영화수는 대조구인 동진벼보다 CNU50 계통에서 2배 이상 많았고, CNU158 계통에서 가장 적게 나타났다. 5. 천립중은 CNU113 계통에서 30g이상으로 대조구와 같이 가장 무거웠고, CNU128 계통에서 20g으로 가장 적었다. 6. 주당 수량은 대조구의 26.6g에 비해 CNU50, CNU112 계통에서 두배정도 높았고, CNU128, CNU158, CNU200 계통에서 가장 낮게 타나났다. Aims of this study were carried out to develop the useful lines induced from mutation and pedigree breeding methods among the collected genetic resources from national and domestic areas. In this study, Stem height of CNU126 line and check among them were high, while CNU128 was lower than other lines. Spiklet length of CNU88 was longer, but that of CNU126 was shorter than check. Number of spiklets per plant of CNU50 among lines have twice time than check. 1,000 grains weight of CNU113 was higher than check. In yield per plant, CNU50 and CNU112 were higher than check, Dongjinbyeo.

겨울철 제주연안 열수지 특성 : 강설현상 snowfall

김경보,장승민,김태희,우종규,윤용훈 濟州大學校 海洋硏究所 2004 해양과환경연구소 연구논문집 Vol.28 No.1

In other to know the characteristics of the air sea heat flux at on the coastal of Jeju Island. when there was a snowfall in winter. It is used as the basic data are Automatic Synoptic Observation System(ASOS) compiled by Jeju Regional Meteorological Office(JRMO) from 1993 to 2002, and SST data derived from National Oceanographic Research Institute(NORI) and National Fisheries Research & Development Institute(NFRDI) observations. the heat flux of the coastal of Jeju Island shows its maximum in January(latent heat flux : 35Wm^(-2) , sensible heat flux : 85Wm^(-2)), and shows its minimum in July(latent heat flux : -l3Wm^(-2), sensible heat flux : -5Wm^(-2)) the more the temperature gap between the air and SST is large and the stronger a wind is, the more it supplies the heat from the sea to the air. it shows that the north-western areas is about three times higher than other areas in Jeju Island. when fresh snow cover is 0.0∼0.9 ㎝, the mean bowen's ratio(for 1993∼2002) is 0.63∼0.67. when it is 1.0∼4.9 ㎝, the mean bowen's ratio(for 1993∼2002) is over 0.72 or more. When bowen's ratio increased upto 0.63∼0.80. fresh snow cover is increased gradually, therefore, it is researched the there is proportion between the fresh snow cover and the bowen's ratio.