Lactobacillus acidophilus 88과 Lactobacillus bulgaricus IFO 13953간의 세포융합주의 특성에 관한 연구

조영배,김혜정,김성구,백형석,전홍기 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

유산균의 균주개량방법의 일환으로 protoplast fusion 기법과 electrofusion법을 이용하여 protease 활성, lipase 활성, 내열성, 내산성 등이 우수한 L. bulgaricus 와 bacteriocin을 생산하는 L. acidophilus를 융합시켜 얻은 융합주들의 생리학적 성질을 검토하였다. 산 생성능, 내열성, 내산성, protease, lipase 활성 등은 L. bulgaricus가 L. acidophilus 보다 우수하였다. L. bulgaricus는 lactose와 sorbose를 이용하였으나 maltose와 sorbitol을 이용하지 못하는 반면, L. acidophilus는 maltose를 이용하고 lactose와 sorbose를 이용하지 못하였다. 융합주 가운데서는 3, 6, 7, 8, 10번이 모균주의 발효능 특성을 함께 지님으로서 재조합체임을 확인 할 수 있었으며, sorbitol의 경우 모균주에서는 발효능이 전혀 나타나지 않았음에도 불구하고 융합주 4, 7번이 발효능을 나타내어 융합과정에서 새로운 형질을 획득하기도 한다는 사실을 알 수 있었다. Lactase 활성은 모균주 모두 높은 β-galactosidase 활성을 보였으나, phospho-β-galactosidase 활성은 거의 없었으며 융합주들도 다소 차이는 있었지만 모균주와 유사한 효소 활성을 나타내었다. 발효에 있어서 key enzyme으로 작용하는 protease, lipase 등의 효소 활성도 모균주의 활성 보다 우수한 융합주도 존재하였다. Aninterspecific fusant was made from the protoplasts of two strains of lactobacillus genus (streptomycin and lincomycin resistant L. bulgaricus and kanamycin resistant L. acidophilus 88). The functional properties of the fusant were examined by determining bacteriocin productivity, acid producing activity, ability of carbohydrates fermentation and three important enzyme activities. The recombinant strain revealed bacteriocin productivity. Acid production and β galactosidase, phospho-β-galactosidase, lipase and protease activity of L. bulgaricus were better than those of L. acidophilus 88. Among fusants, β-galactosidase activity of two strains were better than that of the parent strains but phospho-β-galactosidase activity remarkably lower. One fusant revealed the improved proteolysis compared to that of the parent strains- Lipase activity of L. bulgaricus was better than that of L. acidophilus but another fusant exhibited the highest lipase activity.

SOLID STATE CESIUM ION BEAM SPUTTER DEPOSITION

Hong Koo Baik,Dong Jun Choi,Dong Won Han,Yong Hwan Kim,Seong In Kim 한국표면공학회 1996 한국표면공학회지 Vol.29 No.5

한국의 발톱진균증 환자의 삶의 질에 관한 연구

조백기,박종갑,김형옥,김성욱,백승철,김진우,김시용,안규중,전재복,오지원,김낙인,이규석,오칠환,김수남,김상태,손숙자,신용우,김동석,이원우,권경술,서대헌,황규왕,이종석,고재경,강원형,정기양,최응호,김기홍,박석돈,강승주,함정희,명기범,김방순,구상완,김병수,원영호,김한욱,송은섭,정병수,노병인,홍창권,박장규,한지윤,김광중,구대원,김종민,김재홍,유희준,양경미 대한의진균학회 1998 대한의진균학회지 Vol.3 No.2

Background: Onychomycosis, especially toenail onychomycosis has become one of the common fungal infection and has historically been regarded as a cosmetic rather than medical problem by many patients, even by physicians. Recently, however, there are several reports that is a refractory disease which may cause a deleterious effect on patiets quality of life (QOL). Objective: The purpose of this study was to investigate the impact of toenail onychomycosis on QOL in Korea and to assess the chandes of QOL after treatment. Methods: Total 1004 patients with toenail onychomycosis which was confirmed by clinical findings and KOH preparation were were enrolled at 47 dermatologic centers in Korea, and interviewed with standardized QOL questionnaire before and after sytemic antifungal treatment. Responses to the questionnaire were scored by 5-point scale (1∼4) and averaged, and were analyzed for 5 dimensions of emotional impact, social impact, symptorn and functional impact, patients views concerning treatment, and relationship with doctor. Results: 1. Before and after treatment, the most serious impact was emotional dimension showing 1.90 and 1.30 in average score (AS), and social (AS: 1.14 and 0.83) and symptom and functional impact (AS: 1.05 and 0.92) was also affected. 2. In female rather than male, statistically more significant impact on patients QOL was observed in all dimensions. 3. After treatment, 3 of 5 dimensions were improved significantly - emotional dimension (AS : from 1.90 to 1.30), social dimension (AS: from 1.14 to 0.83), patients view concerning treatment (AS: from 1.34 to 1.02). 4. The drgree of patients satisfaction at the therapeutic effect was very high- 62.4% (immediately after treatment) and 65.8% (9 months affer initiation of treatment) of patients answered excellent or good. Conclusion: This study confirms that toenail onychomycosis has significant impact on the overall QOL of patients. Also the effect of antifungal therapy on patients QOL were watisfactory. Therefore, both doctor and patient should pay more attention to the treatment of onychomycosis. [Kor J Med Mycol 3(1): 115∼124]

Fe-C-Cr 합금의 열처리에 대한 Niobium 첨가의 영향

백홍구 ( Hong Koo Baik ) 한국주조공학회 1989 한국주조공학회지 Vol.9 No.6

Aspirin 投與에 依한 十二指腸粘膜細胞의 微細構造變化

白瑢鉉,洪錫宰,李東久 慶北大學校 醫科大學 1975 慶北醫大誌 Vol.22 No.2

Aspirin 에 의해 惹起되는 十二指腸粘膜 變化를 究明하기 위하여 흰쥐에 常用量인 體重 ㎏當 60㎎을 3, 7, 15 및 30日間 輕口 投與한 후 細胞別로 그 微細構造를 觀察하고 다음과 같은 結果를 얻었다. 吸收上皮細胞에서는 3日째에서 mitochondria의 腫脹과 遊離 ribosome의 增加가 있었으며 15日째부터는 이들 變化가 더욱 甚해지면서 核邊緣의 不規則, microvilli 의 經한 減少, RER의 擴張 및 ribosome脫落 그리고 部分的인 細胞壞死가 나타났었다. 胚狀細胞는 3日째부터 15日째까지는 粘液性顆粒의 大小不同과 融合 等 比較的 經한 變化를 보였으나 30日째에선 顆粒의 消失 내지는 破壞도 나타났다. Enterochromaffin 細胞 역시 30日째에서 分泌顆粒이 減少하고 RER 및 Golgi complex가 擴張되는 소견을 보였다. 未分化細胞에서는 7日째부터 核邊緣의 不規則, microvilli의 減少, mitochondria의 腫脹, RER 및 擴張, 遊離 ribosome의 增加, Golgi complex의 擴張, 細胞間 간격의 이완 등의 變化를 보였으며 15日, 30日째로 갈수록 더욱 甚한 所見을 나타내었다. 또한 30日째에는 細胞質內에서 focal cytoplasmic degradation을 볼 수 있었다. Paneth細胞는 3日째에서부터 分泌顆粒이 減少하는 程度이었으며 投與期間에 따른 差異는 없었다. 以上의 成績으로 보아 aspirrin 의 十二指腸粘膜細胞에 對한 損傷은 주로 吸收上皮細胞와 未分化細胞에서 나타나며, 投與 7日째까진 대부분 可逆性의 輕한 變化를 惹起하나 15日間 以上 投與하면 比可逆性 壞死變化도 誘發하는 것으로 推定된다. The author observed ultrastructural changes of cells in duodenal mucosa induced by aspirin administration. For investigating albino rats were sacrificed after oral administration of 60 mg of aspirin per kg of body weight for 3, 7, 15, and 30 days consecutively. The results were as follows. In the absorptive cells, swelling of mitochondria and increase of free ribosomes were seen on the third day after aspirin administration. With pronouncing the above changes, irregularity nuclear margin, slight decrease of microvilli, dilatation of RER with shedding of ribosomes, and focal cell necrosis occurred on the 15th day. In the goblet cells, confluence, and irregularity in size of mucin granules occurred from the 3rd day to the 15th day. On the 30th day the granules were destructed and disappeared. In the enterochromaffin cells, decreased secretory granules in number and dilatation of RER and Golgi complex were seen on the 30th day. In the undifferentiated cells, irregularity of nuclear margin, decrease of microvilli, swelling of mitochondria, dilatation of RER, increase of free ribosomes, dilatation of Golgi complex, and widening of intercellular space were seen. These changes were more pronounced according to duration of aspirin administration. On the 30th day focal cytoplasmic degradation was appeared. In the Paneath's cells, secretory granules were diminished from the 3rd day. But no changes according to duration of aspirin administration were present. From the above results it is suspected that injuries of the cells in duodenal mucosa induced by aspirin occur mainly in the absorptive cells and undifferentiated cells, and changes that occurred by the 7th day during administration of aspirin are reversible but after the 15th day are necro-tic and irreversible changes.

Alignment Properties of Liquid Crystal Molecules with Negative Dielectric Anisotropy on Hydrogenated Silicon Carbide Films

Koo, Won Hoe,Kim, Jong Bok,Hwang, Byoung Har,Kim, Jong Tae,Jo, Sung Jin,Kim, Chang Su,Lee, Se Jong,Baik, Hong Koo IOP Publishing 2008 Japanese journal of applied physics Vol.47 No.r6

Lewis Acid-Catalyzed Reactions of Anthrone: Preference for Cycloaddition Reaction over Conjugate Addition Depending on the Functionality of α,β-Unsaturated Carbonyl Compounds

Baik, Woon-Phil,Yoon, Cheol-Hoon,Koo, Sang-Ho,Kim, Ha-Kwon,Kim, Ji-Han,Kim, Jeong-Ryul,Hong, Soo-Dong Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.4

The Lewis acid-catalyzed reactions of anthrone with a variety of ethylenic substrates under various conditions have been studied. It has been observed that depending on kinds of ethylenic substrates and catalysts, products were varied. In particular, the $ZnCl_2$-catalyzed reaction of anthrone with ${\alpha},{\beta}$ -unsaturated ester gave bridged compounds 3 (Diels-Alder adduct type) and mono-Michael adduct 4 exclusively, while the base-catalyzed reaction gave 10,10-bis-Michael adduct as a major product independent of the amount of ethylenic substrate and base. Bridged compounds 3 were easily converted to the corresponding mono-Michael adduct 4 by a catalytic amount of base. Further Michael reaction of mono-Michael adducts with different ethylenic substrates in the presence of a catalytic amount of alkoxide gave unsymmetrical 10,10-bis Michael adducts in good or moderate yields.

Colon Transit Time May Predict Inadequate Bowel Preparation in Patients With Chronic Constipation

( Hong Jun Park ),( Myeong Hun Chae ),( Hyun Soo Kim ),( Jae Woo Kim ),( Moon Young Kim ),( Soon Koo Baik ),( Sang Ok Kwon ),( Hee Man Kim ),( Kyong Joo Lee ) 대한장연구학회 2015 Intestinal Research Vol.13 No.4

Background/Aims: We evaluated whether colonic transit time (CTT) can predict the degree of bowel preparation in patients with chronic constipation undergoing scheduled colonoscopy in order to assist in the development of better bowel preparation strategies for these patients. Methods: We analyzed the records of 160 patients with chronic constipation from March 2007 to November 2012. We enrolled patients who had undergone a CTT test followed by colonoscopy. We defined patients with a CTT ≥30 hours as the slow transit time (STT) group, and patients with a CTT <30 hours as the normal transit time (NTT) group. Boston Bowel Preparation Scale (BBPS) scores were compared between the STT and NTT groups. Results: Of 160 patients with chronic constipation, 82 (51%) were included in the STT group and 78 (49%) were included in the NTT group. Patients with a BBPS score of <6 were more prevalent in the STT group than in the NTT group (31.7% vs. 10.3%, P=0.001). Multivariate analysis showed that slow CTT was an independent predictor of inadequate bowel preparation (odds ratio, 0.261; 95% confidence interval, 0.107-0.634; P=0.003). The best CTT cut-off value for predicting inadequate bowel preparation in patients with chronic constipation was 37 hours, as determined by receiver operator characteristic (ROC) curve analysis (area under the ROC curve: 0.676, specificity: 0.735, sensitivity: 0.643). Conclusions: Patients with chronic constipation and a CTT >30 hours were at risk for inadequate bowel preparation. CTT measured prior to colonoscopy could be useful for developing individualized strategies for bowel preparation in patients with slow CTT, as these patients are likely to have inadequate bowel preparation. (Intest Res 2015;13:339-345)

HFCVD법으로 증착된 다이아몬드 박막의 이온주입 효과

백홍구,심재엽,송기문 건국대학교 자연과학연구소 1999 建國自然科學硏究誌 Vol.10 No.1

다이아몬드 에미터는 디바이스 안정성이나 내구성에서 탁월한 성능을 가지므로 합성 다이아몬드 박막에 대한 관심이 크다. 이온주입의 효과가 고온 휠라멘트 화학기상증착법(HFCVD)법으로 성장된 다이아몬드 박막의 다이아몬드 품질에 미치는 영향을 연구하였다. 여기서는 붕소와 인의 이온을 고 에너지로 주입시켜, 이온 주입 전후의 다이아몬드의 구조적 변화를 분석하였다. Raman 스펙트럼 측정에 의하면 붕소 이온 주입된 시료는 많은 흑연 성분을 포함한 미약한 다이아몬드의 특성을 보이고 있으나, 인 이온 주입된 시료는 다이아몬드 구조를 나타내지 않는다. Auger 스펙트럼을 분석하면 붕소와 인 이온을 주입한 시료 모두가 표면에 심한 이온주입 훼손이 생겨 다이아몬드 특성을 잃은 것을 알 수 있다. There has been much interest in synthetic thin film diamond as the emitter materials because the diamond emitter can carry a crucial performance for the device stability and durability. Diamond films grown by hot filament chemical vapor deposition (HFCVD) have been studied to investigate the effect of ion implantation on diamond qualities. Here the diamond films were implanted with boron and phosphorus ions and analyzed their structural changes by implantations. From Raman spectra, the boron ion implanted samples still show a weakened diamond peak with high graphite component while the phosphorus ion implanted sample loses diamond structure. On tile other hand. their AES spectra at surface do not exhibit an obvious diamond shoulder, indicating the surface of ion implanted samples damaged by high fore implantation.

Adipose-derived stem cells ameliorate colitis by suppression of inflammasome formation and regulation of M1-macrophage population through prostaglandin E2

Park, Hong Jun,Kim, Jiye,Saima, Fatema Tuj,Rhee, Ki-Jong,Hwang, Soonjae,Kim, Moon Young,Baik, Soon Koo,Eom, Young Woo,Kim, Hyun-Soo Elsevier 2018 Biochemical and biophysical research communication Vol.498 No.4

<P><B>Abstract</B></P> <P>Inflammatory bowel disease (IBD) is an idiopathic disease caused by a dysregulated immune response to intestinal microbes in an individual with a genetic predisposition. Therefore, alleviation of inflammation is very important to treat IBD. Mesenchymal stem cells (MSCs) have been highlighted as new candidates for treating autoimmune disease based on their immunomodulatory properties. In this study, we investigated the anti-inflammatory mechanism and therapeutic effects of adipose tissue-derived MSCs (ASCs) using THP-1 macrophages and dextran sodium sulfate (DSS)-induced mice with chronic colitis. LPS-treated THP-1 cells expressed mRNA of CD11b, an M1 macrophage marker, at day 2. However, THP-1 co-cultured with ASCs expressed mRNA of CD206, CD68, CCL18, legumain, and IL-10, markers of M2 macrophages. In THP-1 cells co-cultured with ASCs, precursor (pro)-IL-1β, Cox-2, and NLRP3 increased dramatically compared to LPS-treated THP-1 cells. Secretion of IL-1β and IL-18 was significantly inhibited by ASCs, but PGE2 production was highly increased in co-culture conditions of THP-1 and ASCs. IL-18 secretion was inhibited by PGE2 treatment, and PGE2 inhibited inflammasome complex (ASC/Cas-1/NLRP3) formation in THP-1 cells. In the DSS-induced chronic colitis model, ASCs ameliorated colitis by decreasing the total number of macrophages and the M1 macrophage population. Our results suggest that ASCs can suppress the inflammatory response by controlling the macrophage population, and ASCs may be therapeutically useful for the treatment of IBD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> PGE2 was remarkably increased in conditioned media from THP-1 co-cultured with ASCs. </LI> <LI> ASCs induced M1 to M2 macrophage transition through PGE2. </LI> <LI> ASCs suppressed secretion of inflammatory IL-1β and IL-18 through PGE2. </LI> <LI> ASCs ameliorated colitis by decreasing the total number of macrophages. </LI> <LI> ASCs relieved inflammation by decreasing the M1 macrophage population. </LI> </UL> </P>