혈관 생성 억제 제를 투여한 신생마우스 폐 조직에서 Retinoic acid의 세포자멸사의 억제

주선영 ( Sun Young Ju ),조경아 ( Kyoung Ah Cho ),유경하 ( Kyung Ha Ryu ),우소연 ( So Youn Woo ),박은애 ( Eun Ae Park ),조수진 ( Su Jin Cho ) 대한주산의학회 2008 Perinatology Vol.19 No.1

목적: Retinoic acid는 폐포 재생에 중요한 역할을 하는 것으로 알려져 있어서 미숙아의 기관지폐이형성중의 예방에 사용되고 있다. 기관지폐이형성중의 병태생리는 미숙한 폐의 내피세포와 외피세포의 세포자멸사를 일으킴과 관련이 있다. 이에 본 연구에서 VEGFR2 억제가 신생 폐에서 세포자멸사를 증가시키는지, 또 retinoic acid의 투여가 본 폐 발달 억제 동물모형에서 세포자멸사를 억제하는지 알아보고자 하였다. 방법: VEGFR2 억제제인 SU1498을 생후3일된 마우스에 주사하고, 폐포발달이 이루어지는 그 후 10일간 retinoic acid나 위약을 주사하였다. 형태학적인 분석을 하였고, 세표자멸사를 비교분석하기 위해서 TUNEL 염색과 Annexin V을 표지자로 FACS출 시행하였다. 자멸사한 세포를 확인하기 위해서 동일초점 현미경으로 분석하였다. 결과: SU1498를 주사한 마우스에서 폐포면적과 평균폐포용적이 의미 있게 감소하였다. 세포자멸사도 약 3배 의미 있게 증가하였다. 자멸사한 세포는 내피세포와 상피세포에 분포하였다. Retinoic acid를 주사한 약 50%의 세포자멸사가 감소하였고, 형태학적으로 정상에 가까운 폐포 발달이 유지되었다. 결론: VEGFR2 억제를 하면 내피세포와 상피세포의 세포자멸사가 증가되면서 폐 발달이 저해되었다. 이어서 retinoic acid를 주사하면 세포자멸사가 억제되어 폐 발달유지에 도움이 되었다. 이러한 retinoic acid에 의한 세포자멸사의 억제가 기관지폐이형성증 예방에 기여할 것으로 추정된다. Background: Retinoic acid is known to play a role in alveolar regeneration and is used in the prevention of bronchopulmonary dysplasia (BPD) in premature infants. Many factors involved in the pathogenesis of BPD induce apoptosis of the endothelium and epithelium of the premature lung. We hypothesized that VEGFR2 inhibition would increase apoptosis in the newborn lung and retinoic acid would decrease apoptosis in our model of inhibited lung growth. Material and Methods: SU1498, a VEGFR2 inhibitor or vehicle was given to three-day-old mice. Subsequent retinoic acid or vehicle injection was given for ten days for the duration of alveolarization. Morphometric analyses were performed. Apoptosis was assessed with TUNEL staining and Annexin V staining. Co-localization of apoptotic cells with endothelial and epithelial cells was performed. Results: SUI498 injection reduced alveolar surface area and mean alveolar volume in newborn mice. Apoptosis was increased by three-fold in SU1498 injected mice. Apoptotic cells co-localized to endothelial and epithelial cells. Retinoic acid significantly reduced the degree of apoptosis by 50% in SU1498 injected mice and maintained lung development. Conclusion: VEGFR2 inhibition caused an arrest in lung development accompanied by an increase in apoptosis of endothelial and epithelial cells of the neonatal lung in mice. Subsequent retinoic acid treatment reduced apoptosis and we speculate that retinoic acid may preserve lung growth in bronchopulmonary dysplasia by inhibiting apoptosis in the neonatal lung.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1994)

김재홍,안진균,정성재,김영태,김중환,김시영,이석종,이홍렬,서호석,김경훈,권혁진,정우권,고우석,이용석,안필수,오준규,오용섭 대한화학요법학회 1996 대한화학요법학회지 Vol.14 No.1

Background : In recent years gonorrhoea has been panendemic and remains one of the most common Sexually Transmitted Diseases in the world, especially in developing countries. Objective & Method : For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria Gonorrhoeae(PPNG), Ive have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results : In 1994, 168 strains of N. gonorrhoeae were isolated, among which 109 (64,9% ) were PPNG. Conclustion : Our results suggests that the prevalence of PPNG in Seoul is still increasing.

임상별 林間人蔘 의 生育과 最適環境에 관한 硏究 (Ⅰ)

우수영,이동섭 한국농림기상학회 2002 한국농림기상학회지 Vol.4 No.2

The best environments such as crown density, temperature, light intensity and humidity have to be identified because these factors are strongly related to the growth and several physiological characteristics. The purposes of this study are ① to collect basic data for growth, ② to identify the best growth environments. To achieve these purposes, oak, pine and mixed forest stands have been selected in this study. Forest ginseng seeds were sown in these forest four years ago. Several environmental and growth factors have been surveyed. In general, mean tree age, DBH and average height are 20-25 years old, 14-17cm and 7-9m, respectively. The growths of forest ginseng grown in oak stand are better than those of pine and mixed stands.

인공 피음처리에 따른 전나무의 생장과 엽록소 함량변화에 관한 연구

우수영,이동섭,권오규 한국농림기상학회 1999 한국농림기상학회지 Vol.1 No.2

This study was conducted to compare seasonal changes of height, diameter at root collar, biomass and chlorophyll contents of Abies holophylla seedlings grown in Sangju National University Nursery under different light intensities. Four light intensities were adopted using nylon net such as 100% sunlight (1800 μmol m^-2 s^-1), 75% of sunlight (1350 μmol m^-2 s^-1), 50% of sunlight (900 μmol m^-2 s^-1) and 25% of sunlight (450 μmol m^-2 s^-1). Planting density in four light intensities was 30 cm × 30 cm. In each treatment, 100 trees were planted and height, diameter at root collar, biomass and chlorophyll contents were measured. The highest height, diameter at root collar and biomass in Abies holophylla seedlings were observed at 100% full sunlight. Lowest chlorophyll contents (Chlorophyll a, b and total) were shown at 25% of sunlight, lowest light intensity treatment in this study. Growths and chlorophyll contents of Abies holophylla seedlings were strongly associated with light intensity. Photosynthesis has to be investigated in near future because photosynthesis and chlorophyll contents were strongly related to tree growth with long periods.

담도에 국한되어 증상을 유발한 석회쓸개즙 2 예

윤성우,이지영,하경호,이수경,한나,배효근,박선자 고신대학교 의과대학 2010 고신대학교 의과대학 학술지 Vol.25 No.2

Limy bile is a rare condition characterized by excessive precipitation of calcium carbonate in the gallbladder or bile duct. Recently we experienced two cases with obstructive jaundice in which limy bile in the common bile duct but not in the gallbladder for one case, and in the common bile duct and intra hepatic duct but not in the gallbladder for another case. The patients were treated by endoscopic sphincterotomy. Here, we report the cases and review the literature.

가중나무, 튜립나무, 양버즘나무 苗木을 오존에 露出 시켰을 때 葉綠素含量과 Glutathione Reductase 活性變化

禹秀泳,李成漢,權琦遠,李在千 한국임학회 2004 한국산림과학회지 Vol.93 No.7

이 연구의 목적은 대기오염 가운데 오존이 식물에 미치는 영향과 오존 스트레스의 매독 메카니즘에 중요한 기능을 하는 호소의 역할을 구명하기 위래서 도심에 가로수로 많이 사용하는 가중나무, 튜립나무 그리리고 양버즘나무 묘목을 150ppb의 농도로 오존에 하루 8시간, 34일 동안 노출 시켰다. 그리고 가시적 피해현상, 엽록소함량, glutathione reductase 활성을 측정하였다. 가시적인 피해현상은 야버즘나무가 가장 늦게 나타났고, 튜립나무가 가장 먼저 나타났다. 그러므로 아마도 튜립나무는 오존에 대해서 bioindicator 역할을 할 수 있을 것으로 판단된다. 엽록소 함량은 모든 수종에서 감소하는 경향을 보였다. 또한 glutathione reductase 효소 활성은 세 수종 모두 오존에 노출된 묘목의 경우 활성이 높게 나타났다. The purpose of this study was to identify the effects of ozone pollution on the 3 tree species in controlled environment. Ailanthus altissima, Liriodendron tulipifera and Platanus occidentalis seedlings were exposed in both control and ozone chambers to investigate visible damage, chlorophyll contents, and glutathione reductase (GR) activity. Ozone was fumigated 8 hours for a day with 150 ppb concentration. Liriodendron tulipifera seedlings showed early visible damage on the leaf during ozone exposure. It seemed to be one of the bioindicators of ozone pollution. Ozone exposure generally reduced chlorophyll contents on the leaf of three species. In addition, GR activities of 3 tree species exposed in ozone chamber were higher than those of control in every measurement. It is a typical compensatory strategy of stressed trees.

Cilostazol의 항혈소판 응집작용과 sodium citrate의 농도에 따른 혈소판 응집율에 대한 연구

우수경,김영리,권광일 충남대학교 약학대학 의약품개발연구소 2000 藥學論文集 Vol.16 No.-

Cilostazol, 6- 〔4- (1-cyclohexyl- 1H-tetrazol -5-yl) butoxy〕 - 3, 4-dihydro- 2(1H)- quinolinone, is an antiplatelet/antithrombotic agent that has been used for treatment of chronic peripheral arterial occlusion. Cilostazol was studied for its inhibitory effects on platelet aggregation in vitro in rabbit plasma and in human plasma, and also for its antiplatelet effects ex vivo in rabbit after single oral administration. At time t=0, the rabbits were given a single oral dose of 14 ㎎/㎏ cilostazol. At 1, 2, 4, 8, 12, and 24 hours after drug administration, blood samples were taken for measurements of antiplatelet aggregation effect. Before the study of antiplatelet effects of cilostazol in rabbits, the effect of sodium citrate on platelet aggregation in various concentrations was studied to determine the optimal treatment. Cilostazol produced a potent inhibitory effect on platelet aggregation induced by adenosine diphosphate (ADP) in vitro. The maximum effect on antiplatelet aggregation was 14.4% and was appeared at 1 hour after drug administration. The optimum blood/anticoagulant (3.8% sodium citrate) ratio was 9 to 1, and 16 to 1 in human plasma and in rabbit plasma, respectively.

척수손상 장애인의 자가운전 특성에 관한 연구

김수일,나은우,김덕용,배하석 한국전문물리치료학회 2003 한국전문물리치료학회지 Vol.10 No.2

The purpose of this study was to provide information on driving characteristics in persons with spinal cord injury through basic statistic analysis of the survey results. The survey was administered to 44 drivers with spinal cord injury. The subjects' general, neurologic and driving characteristics were analyzed, as well as the degree of difficulty in using their vehicles between tetraplegia and paraplegia. The results were as follows: thirty-five (79.6%) of forty-four respondents was men. The average age was 35.0 years old and the age at the time of injury was 29.0 years old. Their neurologic characteristics were tetraplegics 12 (27.3%) and paraplegics 32 (72.2%). Among complete lesions, the highest level those who could drive independently was C7. All the vehicles were equipped with special devices, including "power steering", "automatic transmission" and "hand controls". The vehicles for cervical cord injury were equipped with "grip bars" as well as for the degree of difficulty in using their vehicles, all the subjects felt that "moving the wheelchair in and out of their vehicles" was too difficult for them to do. We suggest that the driver training should be an essential part of the rehabilitation program for patients with spinal cord injuries to maximize their mobility in the community. This training seems to be essential in order to modify the standards of the Handicapped Drivers Ability Test and to aid the driver rehabilitation program in the health insurance payment system. Also, the driver rehabilitation training program should include instruction in that moving wheelchairs in and out of vehicles.

도시지역 한방의료이용자의 질병양상 조사연구

강영우,조태현,김병하,남철현,조남춘,안창수,이재홍 慶山大學校 保健福祉硏究所 1997 保健福祉硏究 Vol.1 No.-

To provide the basic data for improving of curative measures in oriental medicine and for developing of health education and preventive program after understanding of classification of diseases in oriental medicine users, questionnaire interview was carried out with city dwellers, over 20 years old, selected randomly in big cities from 2nd Oct. to 11th Nov. 1995. The results were summarried as follows: 1. In the general characteristics of subjects, the highest portion of each part was 61.2% of female, 25.5% of thirties, 28.5% of high school graduate, 63.3% of middle class and 11.8% of lower class in economic status. 31.9% was housewife, 73.5% was merried and 55.1%, 38.1% came from big or small cities each. 2. By 21 classification of diseases, 19.7% was blood disorders, 17.6% was pregnancy & delivery complications and 15.2% was endorine & nutritional disorders. 3. As for age 22.6% of endocrine & nutritional disorders in twenties, 20.8%, 28.1%, 27.8% of blood & defence mechanism disorders in thirties, forties, sixties each and 24.9% of pregnancy & delivery complications in fifties were the higher than the other groups. 4. By educational level, 47.8%, 30.5% of blood & defence mechanism disorders in uneducated and middle school graduate were the higher than the other groups. 5. As for occupation, 20.8%, 32.9% of blood & defence mechanism disorders in housewives and sales each, were the higher than the other groups. 6. By economic status, 20.2% of blood & defence mechanism disorders in middle and high class, were the higher than the other groups. 7. By marital status, 21.3% of endocrine & nutritional disorders in singles, 22.0%, 25.7% of blood disorders in having partners, were the higher than the other groups. 8. As for living areas, 18.2% of pregnancy & delivery complications in big city dwellers, 21.3% of endocrine & nutritional disorders in town dwellers were the higher than the other groups. The distribution of diseases in oriental medicine users wes very different by chracteristics according the results. So It's important and essential project to improve curative technics and to develop.

기계시각을 이용한 강구 자동 검사장치

김윤수,박수우,임병훈,김태균,최병재,박철영,이문락,도용태 대구대학교 정보통신연구소 2008 情報通信硏究 Vol.5 No.1

Metal balls are commonly used in mechanical elements and systems including bearings. If there is a defect in a metal ball, it can cause a serious machine failure. By this reason every balls produced are required to be inspected, and the inspection is usually done by human eyes. However, the inspection by human eyes is always with the risk of mistakes, and a need to automate the inspection process is arisen. This paper describes a design of an automatic inspection system for detecting defects on ball surface. A machine vision system is used, and design process and techniques are described in detail. 강구는 베어링을 비롯한 기계 요소 및 장치에서 널리 사용되는 부품이다. 만약 강구에 결함이 있을 시, 이를 사용하는 기계에서 치명적인 결함이 초래될 수 있다. 따라서 제조된 강구에 대해서는 전량검사가 요구되고 있고, 검사과정은 사람의 육안에 의한 검사가 주를 이루고 있다. 그런데 사람에 의한 검사에는 항상 실수의 가능성이 있으므로, 이를 자동화하는 것이 필요하다. 본 논문에서는 강구의 표면 결함을 자동적으로 검사할 수 있는 장치를 설계한다. 기계시각 장치가 사용되었고, 설계에 따른 구체적 절차와 기술들이 서술된다.