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과초산(過酢酸)에 의한 독일가문비나무의 탈리그린화의 적정조건
김윤수 한국목재공학회 1984 목재공학 Vol.12 No.5
The delignification with peracetic acid in spruce wood (Picea abies Karst)were investigated on the variation of reaction time, temperature and the concentration of peracetic acid (PA). Holocellulose, acid-insoluble lignin in the holocellulose (=residual lignin), the content of alpha-cellulose and polyose fractions A and B were determined. The higher the reaction temperature and the concentration of PA and the longer the reaction time, the more the delignification with PA were accelerated, whileas the treatment at temperature lower than 54℃ resulted in a poor delignification. The content in alpha-cellulose and residual lignin depended on the degree of delignification. The yield of polyoses was, however, mostly unrelated to the conditions of delignification. The losses of mannan and xylan reached 30-40℃ during the reaction. Considering the content of residual lignin and polysaccharide, the following reaction condition may he suitable for the delginification test in laboratory, 5% of PA, 90℃ for 1 hr or 10%, 70℃ 2 hrs.
IBA와 NAA 처리에 의해 생성된 Ethylene이 인삼(Panax ginseng C.A. Meyer) 부정근의 생장과 발달에 미치는 영향
김윤수,한은주,백기엽,Kim, Yun-Soo,Hahn, Eun-Joo,Paek, Kee-Yoeup 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.2
The effect of IBA and NAA on adventitious root cultures of Panax ginseng C.A. Mater were investigated. Results indicated differences in growth and development of the roots according to 5mg/L IBA and 2mg/L NAA. IBA resulted in a normal root development and a higher growth compared to NAA. The roots formed on NAA-containing media were shorter and thicker than those in IBA, showing a hypertrophy of the root tip. NAA induced more than 1.6 times higher ethylene production compared to IBA, which caused inhibition of the root growth. Under the ventilation, in the other hand, on difference was observed in ethylene concentration and the root growth between IBA and NAA treatments. Under ventilation ethylene production was not detected until 10 days of culture, while detected from the initial stage under on ventilation. The results suggested the importance of ventilation during the culture for the growth and development of ginseng adventitious roots.
김윤수,고홍범 한국목재공학회 1996 목재공학 Vol.24 No.1
Present work was undertaken to investigate the origin of milled wood lignin(MWL) in the wood cell wall using immunocytochemical techniques, which can provide the information on the localization of specific antigens(MWL in the present study) to be examined. Spruce MWL dissolved in DMSO and emulsified with Freund adjuvant was injected directly into the mouse spleen. The animals were boostered at two-week intervals after the initial immunization. Blood samples were purifed in standard procedures. The characteristics of antibodies against MWL were tested by indirect ELISA. Visualization of MWL was carried out using conventional indirect immunogold-labelling methods on the ultrathin sections of spruce wood. Immuno-TEM observations showed that the imrnunogold probes were selectively attached to secondary cell walls of spruce wood. The most intense labelling was frequently observed in the S2 layer. In contrast, gold labelling in the lignin-rich regions. such as middle lamella and cell corner was not found. The immuno-TEM provides an indication that spruce MWL originates from the S2 layer.
심리운동 관점의 산림치유 프로그램이 중년의 숲 경험에 미치는 영향
김윤수(Kim, Youn-Soo),이병창(Lee, Byung-Chang) 한독심리운동학회 2021 심리운동연구 Vol.7 No.2
본 연구는 심리운동 관점의 산림치유 프로그램이 중년의 숲 경험 정도에 미치는 영향을 알아보기 위해 진행되었다. 심리운동 관점으로 숲에서 산림치유적 요소를 적용하여 프로그램을 구성하였으며, 중년을 위한 심리운동 관점의 산림치유 프로그램이 숲 경험에 어떤 영향을 미치는지 연구하였다. 이를 위해 경험의 3가지 요소(인지적 자유, 내적 동기, 인지적 유능감)로 이루어진 총 9개 문항의 숲 경험척도를 이용하였다. 본 연구의 측정도구는 숲 경험척도로 2017년 김윤수가 개발한 9문항의 척도로 Deci와 Ryan의 자결성 이론을 근거로 하고 있다. 2019년 9월 2일~9월24일 40~60대 C시에 거주하는 성인을 대상으로 산림치유프로그램을 진행하였다. 1차에 2회기 1회기당 40분씩 총 12회기 심리운동관점의 산림치유 프로그램을 실시하였다. 심리운동 관점의 산림치유프로그램이 인간을 이해하고 환경에 적응하는 신체 기능에 대한 폭넓은 이해를 제시하고자 한다. This study was conducted to investigate the effect of a forest healing program from a psychomotor perspective on the forest experience level of middle-aged adults. The program was constructed by applying forest healing elements in the forest from a psychological exercise perspective, and the effect of a forest healing program from a psychological exercise perspective on the forest experience level of middle-aged adults was studied. This study used the Forest Experience Scale with a total of 9 items consisting of three elements of experience - cognitive freedom, intrinsic motivation, and cognitive competence. It was developed by Kim youn-soo in 2017 and based on Deci and Ryan’s theory of self-determination. From September 2 to September 24 in 2019, the forest healing program from the perspective was conducted for middle-aged adults living in the C city in their 40s and 60s. A total of 12 sessions were carried out, each session lasted for about 40 minutes and two sessions were conducted a day. This study attempted to present a broad understanding of the body s ability to understand humans and adapt to the environment through the forest healing program from a psychomotor perspective.
기계를 다루는 여성들, 여성 운전사 ― 1950年代 영화 《女司機》와 《馬蘭花開》를 中心으로
김윤수 중국어문논역학회 2020 中國語文論譯叢刊 Vol.0 No.46
사회주의 시스템이 형성되어가던 1950년대를 배경으로 하는 《女司機(1951)》의 주인공 孫桂蘭은 기차, 《馬蘭花開(1956)》의 주인공 馬蘭은 불도저 운전사가 되어 사회주의 건설에 참여하겠다는 꿈을 갖고, 고된 훈련 끝에 남성 노동자들과 어깨를 나란히 하는 존재가 되어간다. 훈련소 안의 기계들은 여성에게 새로운 신분과 역량을 부여함으로써 여성 노동자는 자본주의와 가부장주의에 희생된 나약하고 수동적인 존재에서 공적 정체성을 지닌 강인한 영웅으로 변모하게 된다. 이 두 편의 영화는 가장 낙후되고 억압받는 존재였던 여성의 변모를 ‘운전하는 여성 형상’으로 이미지화함으로써 중국 사회주의 사회의 진보와 발전을 입증하고자 하였고, 이는 사회주의 여성형상이 제기되는 방식을 보여주고 있다는 점에서도 매우 흥미롭다. 가장 억압받았던 여성을 국가의 일원으로 호출하는 것은 중국 사회주의 정부의 정당성을 구축하는데 매우 중요한 요소였고, 가장 남성적인 영역이라고 할 수 있는 기차와 불도저 운전사로 활약하는 여성 형상은 중국 사회주의의 진보 및 발전방향을 선전하는데 매우 효과적이었다. 그러나 이 두 영화 속에서 여성 주인공은 표면적으로 남성과 동등한 존재로 인정받을 뿐, ‘국가’라는 또 다른 가부장적 권력에 의해 도구화되는 모습을 보이고 있다. 《女司機》의 경우, 기차 훈련소는 가부장적 권력이 작동하는 장으로, 여성, 기계, 남성이라는 삼각관계 속에서 권력을 가진 남성과 여성 간에 미묘한 힘겨루기가 벌어지지만 여성 간의 다툼으로 갈등이 전이되면서 자연스럽게 남녀 간의 갈등이 협상되는 모습을 보이고 있다. 그리고 기차 운전사를 소망하는 孫桂蘭의 꿈이 남성 권력에 의해 실현되고, 孫桂蘭에게 부여된 여성 노동자로서의 자질이 육체적 강인함, 감정의 절제 및 기계적인 모습으로 재현되는 것에서, 남성 위주의 사회구조가 작동하는 방식을 확인할 수 있다. 《馬蘭花開》의 주인공 馬蘭은 훈련소에서 불도저 운전사로 훈련을 받는 동시에, 남성 노동자들을 보살피는 역할도 함께 수행함으로써 가정과 노동을 병행할 수 있다는 것을 증명해 보였고 이에 노동자로서 인정받을 수 있게 된다. 또한 이 작품에서 드러나고 있는 ‘원초적 자연’과 ‘기계’ 그리고 ‘여성’ 간의 상호성 역시 주목할 만하다. 여성들이 이야기를 나누거나 빨래할 때 등장하는 시냇가, 馬蘭이 불도저 조종 연습을 할 때 배경이 되는 척박한 땅과 산 등은 여성과 자연의 긴밀함을 보여준다. 영화 속에서 자연이 배경으로서의 역할을 하듯 여성 주인공 馬蘭 역시 여성성을 통해 남성 중심적인 훈련소의 남성성을 보완하는 도구적 역할을 하고 있음을 알 수 있다. Both Sun-Guiran who is the main character of a film A Female Driver, and Ma-lan who is the main character of a film Malan Flower Blooms, become a train driver and a bulldozer driver respectively after going through hard training as a being taking rank with male workers, with the dream to participate in the construction of socialism. Lots of machines in the training center give them a new status and competencies. Just as the look of female workers treated as cheap labor symbolizes weak China, the women handling huge machines means strong China. By representing the changed look of women who used to be severely behind and suppressed in the past into an ‘image of women who drive and control machines’, they aim to prove the progress and development of socialist society of China, which is led to the vision of New China. Like this, even though the looks of women shown in those films symbolize strong China, in the process in which Sun-Guiran becomes a train driver after going through difficulties, the qualifications of female workers are represented as physical toughness and assimilation into machine. Just as the nature works as the background in the films, the heroine Ma-lan also plays an instrumental role complementing the masculinity of men-centered training center through the femininity. The process in which women skillfully handle machines that are regarded as the preserve of men, shows a complex image combined with women’s liberation, construction of socialism, and industrialization, and within the narrative, the design of New China is revealed, which is very interesting.
환경적 footprint 분석을 통한 토양경작법과 화학적산화법의 비교
김윤수,임형석,박재우,Kim, Yun-Soo,Lim, Hyung-Suk,Park, Jae-Woo 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.3
In this study, land farming and chemical oxidation of a diesel-contaminated site is compared to evaluate the environmental impact during soil remediation using the Spreadsheet for Environmental Footprint Analysis by U.S. EPA. Each remediation process is divided into four phases, consisting of soil excavation, backfill and transportation (Phase 0), construction of remediation facility (Phase 1), remediation operation (Phase 2), and restoration of site and waste disposal (Phase 3). Environmental footprints, such as material use, energy consumption, air emission, water use and waste generation, are analyzed to find the way to minimize the environmental impact. In material use and waste generation, land farming has more environmental effect than chemical oxidation due to the concrete and backfill material used to construct land farming facility in Phase 1. Also, in energy use, land farming use about six times more energy than chemical oxidation because of cement production and fuel use of heavy machinery, such as backhoe and truck. However, carbon dioxide, commonly considered as important factor of environmental impact due to global warming effect, is emitted more in chemical oxidation because of hydrogen peroxide production. Water use of chemical oxidation is also 2.1 times higher than land farming.
열방성 액정 폴리에스터를 함유한 PET의 유변학적 특성 연구
김윤수 한국유변학회 1991 Korea-Australia rheology journal Vol.3 No.1
상업적으로 많이 이용되는 폴리에틸렌테레프탈레이트(PET)에 액정중합체(LCP)인 열방성 폴리에스터를 첨가하여 유변학적 특성을 조사하고 전단속도와 혼합비에 따른 LCP domain의 형태 변화를 고찰하였다. 모체고분자 내 구형과 타원형을 이루는 LCP domain들 은 신장력에 의해 피브릴 구조의 변형되고 이 피브릴은 흐름방향으로 배향되어 용융체에 윤 활제와 같은 역할을 함으로써 용융점도의 감소를 보이는데 특히 높은 전단속도 영역에서 LCP가 30wt%까지 첨가될수록 큰 폭으로 감소하였다. 주사식 현미경(SEM)의 관찰로부터 LCP domain의 피브릴구조를 확인할 수 있었고 또한 LCP domain의 형태 변화가 용융점도 가 감소에 직접 관계됨을 확인할 수 있었다.
김윤수,황석귀,송정석 ( Yoon Soo Kim,Suk Kuy Whang,Chung Suk Song ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.2
In most mammals, levels of lactase are high during infancy but by the time adulthood is reached these levels of lactase are deficient. So far, the exact mechanism involved in the appearance and disappearance of lactase is not known. The present experimental study describes the digestive enzymatic adaptation of disaccharidase to the substrate in diet composition in order to investigate whether the mere presence of corresponding substrate in the diet suffices to stimulate disaccharidase or not, probably by $quot;turning on$quot; which encodes for the synthesis of these enzymes. Our experimental studies suggest that lactase activity in the newborn of the rat immediately after normal birth is at a peak and remains there through the lactating period; however, disappearance of lactase was observed four days after weaning. It reappeared in seven days after the rat, which had been fed for 150 days on a sucrose diet, was shifted to a lactose diet, However, the activity was weaker than that of the lactating period, indicating that enzymatic adaptation of latose to the substrate in diet may be related. On the other hand no surcease levels were found throughout the lactating period, but the levlel of surcease rose independently of the sucrose diet intake after weaning, indicating that the sucrose in the diet did not seem to influence the surcease activity at all.