영아자(Phyteuma japonicum Miq.)의 성분 조성

정미자,신정혜,이수정,홍성국,강호중,성낙주 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

산채식물인 영아자(Horned Rampion ; Phyteuma japonicum Miq.)의 영양학적인 가치를 평가코져 야생 및 평지재배 시료를 잎과 줄기로 구분하여 일반성분, 비타민 C, 유리당, 무기물, 핵산관련물질, 구성 아미노산 및 유리아미노산을 분석하였다. 야생과 재배 영아자의 회분은 1.2∼2.7%의 범위였고, 조지방과 조단백질은 재배시료가 조섬유는 야생시료에서 더 높은 함량으로 정량되었고, 전당은 두 시료간에 대차를 보이지 않았다. 비타민 C는 줄기보다는 잎에서 재배시료보다는 야생시료에서 더 높게 정량되었다. 유리당은 야생 및 재배시료 모두 glucose, frucose 및 sucrose가 잎보다 줄기에서 높게 정량되었다. 무기물은 총 9종이 분석되었는데 이중 칼슘의 함량이 가장 높아 재배시료의 경우 잎은 34374.0㎎/㎏, 즐기는 9584.1㎎/㎏였고, 그 다음으로 칼륨, 마그네슘의 순으로 많았으며, 야생시료도 비슷한 경향을 보였다. 핵산 관련물질은 CMP, UMP, IMP, AMP 및 hypoxanthine이 동정되었는데 잎과 줄기 모두 야생시료에서는 hypoxanthine이, 재배시료에서는 AMP가 월등히 높게 정량되었다. 구성아미노산은 총 17종으로 야생시료에서는 glutamic acid, 재배시료는 잎의 경우 aspartic acid, 줄기의 경우 glutamic acid의 함량이 가장 많았다. 유리아미노산은 총 29종이 동정되었고 야생시료의 잎에서는 glutamic acid, 줄기에서는 γ-aminoisobutyric acid, 재배시료의 줄기에서는 asparagine이 가장 높은 함량으로 정량되었다. This research is to establish the basic data of the nutritive value and improve our diet. In the part of th leaf and stem of the wild cultivated horned rampion(Phyteuma japonicum Mig), the components such as chemical composition, vitamin C, free sugar, mineral, nucleotide and its related compounds, composition and free amino acid were analyzed one after another. Content of the crude lipids and proteins was determined much higher in its wildness than in its cultivated horned rampion ; while, that of carbohydrates was higher in the former than in the latter. The content of vitamin C was retained higher in the leaf than in the stem horned rampion. And the content of calcium among the detected minerals was outstanding in all of the samples collected, and potassium and magnesium was the next ones in its order. The main components of free sugars in both the wild and cultivated horned rampion were glucose and fructose, and their content was higher in the stem than in the leaf. Nucleotide and its related compounds were identified with 5 kinds of nuciotides such as CMP, UMP, IMP, AMP and hypoxanthine(Hx), and the content of Hx and AMP was the highest in the wild and cultivated samples, respcetively. In the composition amino acid of the wild horned rampion, glutamic acid, aspartic acid and phenylalanine was outstandingly abundant; while, such amino acid as methionine and proline was small and besides cysteine couldn't be detected in the stem. Total amounts of composition amino acid in the leaf was 2118.0 and 1120.1㎎% in the wild and cultivated sample, respectively. In the free amino acid of horned rampion, the total amount ranged from 8.5 to 50.1㎎%, which were lower level than that of composition amino acid. But the number of free amino acid was 29 kinds, which was bigger in its number than of composition amino acid detected 17 kinds.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

중수화를 위한 우수처리에 관한 연구

정병길,김미향,박성현,박광현,성낙창 東亞大學校 建設技術硏究所 2005 硏究論文集 Vol.28 No.2

The objectives of this study are to evaluate the pH neutralization and NO_(3)^(-), SO_(4)^(2-) adsorption according to the quantity of acid rain, particle diameter and packing height of oyster shells. Acid rain is mainly of anthropogenic origin due to the strong presence of SO_(2) and NOx gases in the atmosphere. Acid rain causes environmental damages, such as lake acidification, negative effects on the vegetation, in particular forests, changes in the chemical composition of solids and in metal mobility. Neutralization of artificial acid rain was enhanced by smaller particle diameter of powdered oyster shells and larger reaction area of packed column. At the initial 12 hours of reaction time, the pH of acid rain increased rapidly. pH neutralization was appeared the highest at the 48 hours of reaction time. The removal of NO_(3)^(-) was observed and the R3 reactor showed highest efficiency of 27.8%. But, SO_(4)^(2-) removal did not observed in this study.

제초제(propanil. butachlor) 저항성 잡초 선발 방법에 관한 연구

정성엽,김영미,박재현,이재현,김학윤,이인중,신동현,김길웅 경북대학교 농업과학기술연구소 1999 慶北大農學誌 Vol.17 No.-

This study was conducted to develop a simple selection method for herbicide-resistance weeds. Two methods, designated "seedling method" and "stem node method" were employed for screening of barnyardgrass against propanil and butachlor. In the seedling method, shoot and root growth of barnyardgrass were significantly inhibited at quarter of the recommended herbicide rate, while in the stem node method, the similar inhibition was obtained at half of the recommended rate. Thus, it was concluded that the seedling method is more simple and quick method to evaluate response of barnyardgrass to propanil and butachlor compared to stem node method.

전립선 기질세포의 증식과 COX-2 발현에 대한 프로게스테론의 영향

정수련,김성한,최이화,박지은,전은미,강영진,이광윤,최형철 영남대학교 의과대학 2006 Yeungnam University Journal of Medicine Vol.23 No.1

전립선비대증은 노인 남성에서 흔히 유발되는 질환이며, 노화가 진행될 수록 빈도가 높아지는 특징을 가진다. 이 질환의 원인은 전립선기질세표의 과도한 증식으로 유발된다고 알려져 있지만 그 자세한 기전에 대해서는 잘 알려져 있지 않다. 전립선비대증에서 progesterone 수용체 양성 세포가 다른 전립선 종양에 비해서 많고, progesterone은 testosterone에서 DHT로 전환되는 것을 감소시키는 역할을 가진다고 알려졋다. 또한 남성 전립선 평활근의 과증식에 의한 질환이므로 평활근 세포의 증식과 관련성이 있다고 보고된 COX-2의 전립선비대증에 대한 영향에 대한 연구가 필요하다. 전립선 기질세포에 progesterone을 3일간 투여하여 배양한 경우 기질세포 증식은 차이가 없었다. Progesterone을 단독 또는 DHT와 같이 투여한 기질세포에서 남성호르몬 수용체 mRNA 발현은 비처리군과 비교하여 유의한 차이가 없었다. 또한 progesterone과 DHT 동시 투여에 의한 COX-2 mRNA 발현에도 차이가 없었다. 그러나 progesterone에 의한 남성 호르몬 수용체와 COX-2 단백 발현에서는 대조군과 비교하여 유의하게 감소시켰다. 이상의 결과는 progesterone은 남성호르몬 수용체에 대해 전사 후 반응 (post-transcriptional response)에 효과를 나타내어 남성호르몬 수용체 발현을 감소시키는 작용은 가지며, COX-2 발현 억제효과를 나타내므로 전립선비대증의 치료에 이용될 수 있을 것으로 사료된다. Background: Benign prostatic hyperplasia (BPH) is the most common benign tumor in older men; the etiology of this disease remains poorly understood. Testosterone and dihydrotestosterone (DHT) both act as androgen via a single androgen receptor. Testosterone is converted to DHT by 5α-reductase in prostatic stromal cells. Progesterone has been reported to inhibit DHT conversion; howevwe, its effect on prostatic stromal cells remains to be elucidated. Materials and Methods: In this experiment, we investigated the effect of progesterone on androgen receptor expression induced by DHT. We also tested the effect of progesterone on cyclooxygenase-2 (COX-2) expression, as well as prostate stromal cell proliferation using the cell count kit-8. Results: Progesterone did not cause an increase of prostate stromal cell proliferation. The mRNA expression of the androgen receptor and COX-2 were not changed by progesterone; the expressions of androgen receptor and COX-2 proteins were decreased by progesterone in prostate stromal cells. Conclusion: These results suggest that in prostate stromal cells, progesterone decreases androgen receptor protein expression, which results in decrement of COX-2 protein expression. This effect might be mediated by post-transcriptional regulation.

감염된 가축과의 직접 접촉 없이 발생한 브루셀라증 환자1례와 축산 부산물 시장에서의 역학조사 : Sero-epidemiology Study on Market, by-product, Stock-raising

정성주,김성범,박대원,정혜원,기세윤,권정아,박만석,박미연,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

브루셀라증은 전 세계적으로 발생하는 인수공통 전염병중에 하나로서, 본 증례는 국내에서 감염된 가축에 대한 노출력이 없는 집단에서 현증 브루셀라증이 발생한 첫 증례이기에 문헌 고찰과 함께 보고한다. 또한 환자가 근무하였던 축산물 부산물 시장에서 역학 조사를 실시하여, 현증환자 및 기감염자를 발견하여 축산물 부산물 시장 근무자에서 현증 브루셀라증이 발생할 위험성을 평가하였다. 역학 조사 결과 축산물 부산물을 다루는 일을 하는 사람들에서 감염된 가축과의 직접 접촉이 없이도 브루셀라증이 발생할 수 있음을 알 수 있었다. Brucellosis is a world-wide zoonotic disease. We report the first case of brucellosis in South Korea that is not related to direct contract with intected cattles. The patient had been working in the market of by-product, stock raising for twenty years and complained of 1-month history of fever, chill, and intermittent epistaxis. We confirmed brucellosis by anti-brucella antibody test and PCR. After 6 weeks' treatment with rifampicin and doxycycline, fever and chilling subsided, and, generalized weakness and oral intake improved. We performed seroepidemiology study against workers who were working in the same market as the patient. However, we couldn't find out other cases with seropositivity. We concluded that although the workers of by-product market have a risk of getting brucellosis, the risk is low.

중기발암성시험기법을 이용한 Carbendazim의 간발암성 검색

성하정,이제봉,정미혜,이해근 한국환경독성학회 1998 환경독성보건학회지 Vol.13 No.2

Carbendazim, which is widely used fungicide, was investigated for rat hepatocarcinogenesis using a medium-term carcinogenicity bioassay. All rats were initially given a single dose (200 mg/kg) of diethylnitrosamine (DEN) i. p. and then, starting 2 weeks later, carbendazim treatment group and positive control group received carbendazim (7 mg/kg/ day) and 2-acetylaminofluorene (2-AAF, V/0, respectively, in the diet for 6 weeks. All rats were subjected to two-thirds partial hepatectomy (PH) at week 3 and sacrificed at week 8. Carcinogenic potential was scored by comparing the number and area per cm" of induced glutathione-S-transferase placental form (GST-P) positive foci in the liver. Carbendazim had no effect in the increase of body weight, hematological and biochemical values, and the number and area of GST-P positive foci. These results suggest that this bioassay using DEN-PH method can be useful for detection of hepatocarcinogenic potentials of pesticide.

전북지역 고문서 연구현황과 그 활용방안

정성미 원광대학교 인문학연구소 2005 열린정신 인문학연구 Vol.6 No.-

The purpose of this thesis is to show the actural and present status of the study on old documents owned by Chonbuk province and make a few suggestions about how to make use of them. Old documents are valuable not only as historial materials themselves, but as materials for cultural use as to a study on Korean history. That's why we should collect, document, and preserve the old documents being lost through modernization and industrialization. This thesis consists of two categories. One category is about the present status of the researching and documenting of old documents designated as cultural assests and those which are not. In case of the old ducuments not designated as culturas assets, the status of documenting and researching using old documents is broken into parts according to the places having them. The other is about the ways of aplication using old documents. The way how to store old documents in the computer is presented, including methodology of the regional history and the way of developing th regional culture contents using who's who or a gazetter. The materials used here come from old documents in Iksan region conducted by Iksan Historical Materia! Is Committee.(益山史料조調査委員會) There are 695 old documents designated as cultural assets including those designated as cultural assets by Chonbuk province itself, and 31,575 old documents not designated as cultural assets in Chonbuk. There are also approximately 2,663 old documents belonging to each individual, which are divided into the documents related to a state examination and a government post (42%), a etition and a lawsuit (19%), a family register (19%), and a personal property (14%). The amount of old documents owned by each museum account for more than 90% of the documents belonging to Chonbuk province, and, especially, the Museum of Chonbuk National University has about 18,000 old documents accounting for 57%. Most of the old documents carried in literature are no more than an introduction toan event or a person and they are not fully interpreted or documented. The National History Compilation Committee has comparatively carried out research on historical materials about Iksan in detail by adding images and detailed lists. Also, Museum of Chonbuk National University ha published books about old documents. In addition, of the writings using old documents in Chonbuk province, those referring to Buan Kim's documents(扶安金氏古文書) of the families from the same clan account for the most However, these are likely to change because a research and a study on old documents being underway are not included. Lastly, these findings are based on the condition of the r! esearch and study conducted on September, 2004.

적혈구 유무에 따른 벤젠, 스티렌, 트리클로로에틸렌에 의한 림프구 중 자매염색분체 교환 빈도

성재혁,김형아,신민정,최미정,이세훈 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.4

This study was performed to investigate the possible role of oxyhemoglobin in the metabolic activation of benzene, styrene and TCE by observing the difference of the SCE frequency between isolated lymphocyte and whole blood lymphocyte cultures exposed to these chemicals. Isolated lymphocyte and fresh heparinized peripheral whole blood samples from a healthy donor(male, smoker) were cultured in the phytohaemagglutinin stimulated culture media for 72 hours. The cells were treated with 100, 300, 1000, 3000 μM benzene, 250, 500, 100, 2000 μM styrene or TCE respectively. Slides were stained with Giemsa's solution for SCE. SCE was analyzed for each subject from coded slides by one researcher. The results were as follows : 1.The frequency of SCE increased dose-dependently with concentration of benzene in isolated lymphocyte and whole blood lymphocyte cultures, however there were no significance. There was no significant difference of SCE frequency between isolated lymphocyte and whole blood lymphocyte cultures. 2.The frequency of SCE in whole blood exposed to sytrene significantly increased in dose-dependant relationship, but not in isolated lymphocyte. The difference of SCE frequency between isolated lymphocyte and whole blood lymphocyte of 2000μM styrene exposure was marginally significant(P=0.0540). 3.In TCE, the frequency of SCE did not increase at any concentration used in this study of isolated lymphocyte and whole blood lymphocyte. Above results suggested that oxyhemoglobin in erythrocytes play an important role in the metabolic activation of styrene but not in benzene or TCE. Key Words : Benzene, Styrene, Trichloroethylene, Sister chromatid exchange, Metabolic activation, Oxyhemoglobin

건강, 교육, 취업 현황에 대한 여성장애인의 인식과 성인지적 관점에서 본 욕구 : 사회단체 회원인 여성장애인을 대상으로 focused on health, and employment

성정현,이영미 한국장애인재활협회 2003 재활복지 Vol.7 No.1

본 연구에서는 장애인 실태조사에서 나타난 건강, 교육, 취업영역의 성별 통계의 차이를 여성장애인이 어떻게 인식하고 있는지 알아보고 이와 같은 격차를 해소하기 위해 우선적으로 충족되어야 할 여성장애인의 욕구를 파악하고자 하였다 이를 위해 6명의 여성장애인과 면접을 실시하였으며, 여성장애인 164명을 대상으로 설문조사를 실시하였다. 연구결과, 여성장애인은 건강, 교육, 취업에서의 성별 통계의 차이를 가부장적 관념과 남성중심의 사회구조에서 비롯된 차별로부터 기인하며, 차별은 취업과 교육, 건강의 순으로 심각하다고 인식하였다. 성별로 인한 차이를 해소하기 위해 건강분야에서는 일상적인 건강과 관련된 가사, 육아도우미 서비스의 제도화와 산후조리서비스, 임신 및 출산문제에 대한 지원과 같은 전통적인 성역할과 관련된 실질적인 욕구를 갖고 있었으며, 그와 함께 건강교육 및 정보제공, 전문병원의 설립, 건강비용의 감면 등을 통해 이동의 제한이나 경제적 어려움이 남성장애인보다 더 큰 현실을 근본적으로 해소해줄 것을 원했다. 교육에서도 성별을 고려한 취업교육과 이동권을 보장을 원했으며, 학업을 중도포기하는 여성장애인이 다수인 점을 고려한 검정고시반 설치를 원한다. 또한 이동의 문제를 고려한 취업직종의 개발과 직원의 인식개선, 여성상담원의 배치에 대한 욕구가 높게 나타났다. 이와 같은 건강, 교육, 취업에서 여성장애인들은 일관되게 비전통적 인 성역할을 지향할수록 욕구가 높아지는 것으로 나타나 여성장애인의 의식과 행동에 성역할 태도가 중요한 변수임을 확인할 수 있었다. Women with disabilities face a double discrimination due to their gender and disabilities. To monitor and evaluate the impact of policies on disabled women, it is important to seek perspectives of women with disabilities. The purpose of this study is to describe the understanding of the real situations of Korean women with disabilities and investigate the need for gender mainstreaming. The three main policies for women with disabilities were reviewed: health, education ,and employment. The study combines analysis of focus group interview data and survey questionnaires. The study reveals the following results: first, more than 50% of the disabled women in this study experienced discrimination.; second, women with disabilities in this study feel the need of the gender sensitive policies; disabled women who have modern sex role attitudes demand more need than those who have traditional sex role attitudes.