Rat의 복강에 삽입한 Filorouracil-Polyglycolic acid제형의 Fluorouracil용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitioneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adenocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

Trace-forward만을 사용한 Viterbi Decoder

노승효,오승훈,이찬호 崇實大學校 1999 論文集 Vol.29 No.1

Recently, the Viterbi algorithm is widely used for the decoding of convolutional codes. This paper proposes a Viterbi decoder using a trace-forward processing to reduce the amount of memory storing the survivor path information and to increase the decoding speed. We adopt fully parallel structure for ACS(add-compare-select) units to minimize the delay which determines the Viterbi decoder's throughput. We reuses ACS units to reduce ACS area that occupies the majority of the chip area. The decoder is designed using VHDL and is implemented using a CPLD. It is verified to operate by a measurement using a logic analyzer.

위선암의 Billroth Ⅰ 수술시 수기문합과 자동문합의 비교

노승무,나호청,윤완희,장일성,배진선 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

The aim of this study was to establish wheather the EEA stapler method was a secure and simple procedure for gastroduodenostomy in the stomach cancer, without increased morbidity and mortality rates compared with the manual method. During the period September 1990 to August 1993, a stapler method (n=86) or manual method n=38) was performed in 124 patients. The results were as follows ; 1) Distal one third of the stomach was prevalent site of tumor(73.4%). On gastric antrum located tumor, manual and stapler method was performed on the 32 cases(84.2%) and 59 cases(68.6%), respectively. On gastric body located tumor, manual and stapler method was performed on 6 cases(15.8%) and 27 cases(31.4%), respectively. 2) The radical and palliative resection rate of manual method was 35 cases(92.1%) and 3 cases(7.9%). The radical and palliative resection rate of stapler method was 81 cases(94.2%) and 5 cases(5.8%). 3) Major complications of the anastomotic site were stenosis and bleeding. Two cases of stenosis were observed in the stapled group(2.3%), and no case in the manual group. One case of bleeding had occured in the stapled group(1.2%), and no case in the manual group. 4) The average operation time of manual and stapler method was 269 minutes and 265 minutes, respectively. 5) The average hospital stay of manual and stapler method was 18.5 day and 19.0 day, respectively.

Rat의 복강에 삽입한 Fluorouracil-Polyglycolic acid 제형의 Fluorouracil 용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitoneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adcnocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.

V₂O-B₂O₃-PbO 유리의 전자 상자성 공명연구

노태호,전인,박영민,강재필,김성재,박현진,송승기 명지대학교 자연과학연구소 1996 자연과학논문집 Vol.14 No.-

V₂O??-B₂O₃-PbO 유리의 VO??에 관한 전자 상자성 (EPR) 스펙트럼으 연구하였으며, vanadium이온의 스핀해밀토니안 상수들을 구하였다. 유리시료안의 V₂O??양을 증가시킴에 따라 g??과 g??값은 증가하였으나 A??과 A??값은 감소하였다. 이는 V₂O??수가 증가하면 동일 평면상의 vanadium과 oxygen배위간 결합의 공유성이 증가함을 의미한다. 유리내의 vanadium 4가이온은 정방형 일그러진 8면체를 한 vanadyl착물로 존재한다. Electron Paranmagnetic Resonance(EPR) SPECTRA OF (VO)?? in V₂O??-B₂O₃-PbO glasses have been studied and spin hamiltonian paramenters of vanadium ion have been calculated. It is found that the value of g??and g?? increases while the value of A?? and A?? decreases with an increase of V₂O?? content in the glass samples, showing that the covalency in the in-plane vanadium -oxygen ligand bonds increase with increasing the concentration of V₂O??. The tetravalent vanadium ions exist as vanadyl complexes with tetragonally distorted octahedral sites in the glasses.

견관절 재발성 탈구에서 관절경적 견관절 전방 고정술의 결과 : 2년에서 6년간의 추시 관찰 Two to Six-Year Follow-up

김승호,하권익,노규철,류병담,오일빈 대한스포츠의학회 2003 대한스포츠의학회지 Vol.21 No.1

The purposes of this study were to prospectively evaluate the surgical otucome of the arthroscopic repair of the capsulolabral lesion using suture anchors in al large series of patients who were followed-up for mid-term periods. We evaluated 167 patients with traumatic recurrent anterior shoulder instability after an arthroscopic Bankart repair using suture anchors and nonabsorbable sutures. The mean age at the time of the operation was 25 years (15~46 years). There were 149 male and 18 female patients. Preoperatively and at the time of follow-up(mean, 44 months; range, 24~75 months), three objective outcome measurement tools(Rowe Score, University of California at Los Angeles Shoulder rating scale, and American Shoulder and Elbow Surgeons score) and two subjective measurements(pain and function visual analogue scale) sere assessed. The recurrence rate, range-of-motion, and risk factor for postoperative recurrence were evaluated. All shoulder scores improved after surgery(t-test, p<0.05). According to the Rowe scale, One hundred and thirty patients(78%) showed excellent, 29(17%) good, 6(4%) fair, and 2(2%) poor scored. Overall, postoperative recurrence of the instability was 4%(1 dislocation, 2 subluxation, and 4 positive anterior apprehensions). Postoperative recurrence was related with the glenoid defect of greater than 30%(Gehan's test, p<0.0001). In patients with postoperative instability, the recurrent episode was less frequent and shoulder function was related with the activity were increased after the surgery(t-test, p<0.001). One hundred and fifty-two patients(91%) returned to more than 90%of previous activities. The mean loss of external rotation was 2.0±4.0℃. In contrast to the previous reports on the results of the arthroscopic repair, arthroscopic capsulolabral repair using suture anchors provided satisfactory outcomes in terms of the recurrence, activity, and range of motion.

Cadmium Sulfate가 肝組織에 미치는 影響에 관한 形態學的 硏究

윤강호,박경란,김수일,노승무,김원식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

Cadmium is well known toxic heavy metal which has been related with various pathologic conditions of liver, kindney, testis, cardiovascular system and central nervous system etc.. In this experiment, cadmium sulfate (1.2mg/100gm b.w.) was given intravenously into the mouse (Balb - C, b. w. 20 - 25gm) via tail vein for the study on morphological changes of liver. On the 8hours, 16hours and 24hours after administration, mice were sacrificed under ether anesthesia, liver was removed, then, a part of liver was fixed in 10% neutral buffered formalin solution, processed routinely, stained with hematoxylin & eosin, and observed under light microscope. Otherwise, the remainder portion of liver tissue was prefixed in 1.5% glutaraldehyde solution, postfixed in the 1% osmium tetraoxide solution, embedded with Epon 840, and observed under transmission electron microscope(H-7000, Hitachi Co.). The results obtained were as followings ; 1. On light microscopic observation, hemorrhagic necrosis at the zone Ⅰ and zone Ⅱ with congestion of hepatic lobules were found. 2. On transmission electron microscopic observation, cisternal dilation of RER & SER, autophagic vacuoles, myelin figures, and certain electron dense materials within the hepatocytes were found. Although this observations were limited in mice, the above findings of its acute effects on liver morphology will serve morphological basis for the better understanding of toxic effects on liver after chronic exposure or multiple interactions with other toxic substances.

파라콰트 중독 흰쥐에서 항산화제 U-74389G 의 치료 효과

오진호,정성필,임훈,노성훈,김혜영,김승호,이한식,민진식 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: This study was conducted to verify the hypothesis that the suppression of lipid peroxidation with the antioxidant, U-74389G, could improve the survivability of paraquat intoxicated rats. Methods: First, we obtained the 24-h mortality by using several paraquat dosages and calculated the 24-h LD5O in 24 male Wistar rats(250~350 g). To examine the effect of U-74389G, we divided the rats in 4 groups: a control group and U-74389G only group, a paraquat only group, and a paraquat plus U-74389G group(n=10 each). Paraquat, 35 mg/kg, was injected intraperitoneally at 0 h. U-74389G, 10mg/kg, was administered intraperitoneally at 0, 12 h or at 1, 12 h in the respective groups. The rats were observed for 24 hours. At 24 h, plasma and lung, liver, and kidney tissues were obtained after sacrificing the surviving rats to determine the degree of lipid peroxidation by using a thiobarbituric acid reactive substances (TBARS ) quantitative analysis. Results: The 24-h LD50 of paraquat was calculated as 40 mg/kg in our rats. The 24-h mortality was as follows: control group and U-74389G group 0%, paraquat group 30%, and paraquat plus U-74389G group 10%. The TBARS analysis showed no differences between the U-74389G and the control groups. The paraquat group showed significantly increased TBARS levels in the serum and in the kidney and lung tissue compared to the control group(p<0.05). With U-74389G, the increased TBARS levels were significantly decreased in the plasma, kidney, and lung tissues compared to the paraquat group(p<0.05). However, in the liver tissue, there were no significant differences among the groups. Conclusion: A 21-aminosteroid antioxidant, U-74389G, improved the survivablity of paraquat-intoxicated rats through the suppression of lipid peroxidation. Our result suggests the possibility of clinical application of this drug as an antidote for paraquat poisoning.

Aflatoxin B_1과 Dexamethasone이 白鼠 肝 Kupffer 細胞와 內皮細胞에 미치는 影響에 關한 走査電子顯微鏡的 硏究

이영호,김수일,박경란,노승무,김원식 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.1

For the study on the effects of aflatoxin B_1 and corticosteroid to the Kupffer cells and endothelial cells of the liver, aflatoxin B_1(1mg/kg) and dexamethasone(4mg/kg) were administrated to the 3 groups of rats; the aflatoxin B_1-treated group, the dexamethasone-treated group, and the aflatoxin B_1 and dexamethasone-treated group. The liver tissues were fixed by perfusion with 0.5% glutraldehyde and 0.5% paraformaldehyde solution at 1, 12, and 24 hours after the administration. The fixed liver tissues were cracked and observed Kupffer cells and endothelial cells under scanning electron microscope. The results were as follows; 1. In aflatoxin B_1-treated group, the Kupffer cells were destructed severely and the endothelial cells were destructed partially. 2. In dexamethasone-treated group, the cytoplasmic processes of the Kupffer cells were shortened, but the endothelial cells showed no morphological changes. 3. Destructive changes of Kupffer cells and endothelial cells in the aflatoxin B_1-treated group were reduced after administration of dexamethasone. 4. The large Kupffer cells were destructed and disappered after administration of aflatoxin B_1, but small Kupffer cells were found until 24 hours after administration. 5. In aflatoxin B_1 and dexamethasone treated group, Kupffer cells were destructed severely but the capacity to catch foreign particles were remained. Accordingly, it is considered that aflatoxin B_1 will destruct Kupffer cells severely and the endothelial cells partially, but dexamethasone will reduce the destructive changes of the Kupffer cells and endothelial cells induced by aflatoxin B_1 respectively.