In vivo transduction of ETV2 improves cardiac function and induces vascular regeneration following myocardial infarction

Lee, Sunghun,Lee, Dong Hun,Park, Bong-Woo,Kim, Riyoun,Hoang, Anh Duc,Woo, Sang-Keun,Xiong, Wenjun,Lee, Yong Jin,Ban, Kiwon,Park, Hun-Jun Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.2

<▼1><P>Vascular regeneration in ischemic hearts has been considered a target for new therapeutic strategies. It has been reported that ETV2 is essential for vascular development, injury-induced neovascularization and direct cell reprogramming of non-endothelial cells into endothelial cells. Thus, the objective of this study was to explore the therapeutic potential of ETV2 in murine models of myocardial infarction in vivo. Direct myocardial delivery of lentiviral ETV2 into rodents undergoing myocardial infarction dramatically upregulated the expression of markers for angiogenesis as well as anti-fibrosis and anti-inflammatory factors in vivo. Consistent with these findings, echocardiography showed significantly improved cardiac function in hearts with induced myocardial infarction upon ETV2 injection compared to that in the control virus-injected group as determined by enhanced ejection fraction and fractional shortening. In addition, ETV2-injected hearts were protected against massive fibrosis with a remarkable increase in capillary density. Interestingly, major fractions of capillaries were stained positive for ETV2. In addition, ECs infected with ETV2 showed enhanced proliferation, suggesting a direct role of ETV2 in vascular regeneration in diseased hearts. Furthermore, culture media from ETV2-overexpressing cardiac fibroblasts promoted endothelial cell migration based on scratch assay. Importantly, intramyocardial injection of the adeno-associated virus form of ETV2 into rat hearts with induced myocardial infarction designed for clinical applicability consistently resulted in significant augmentation of cardiac function. We provide compelling evidence that ETV2 has a robust effect on vascular regeneration and enhanced cardiac repair after myocardial infarction, highlighting a potential therapeutic function of ETV2 as an efficient means to treat failing hearts.</P></▼1><▼2><P><B>Cardiovascular disease: New hope for healing the heart</B></P><P>A gene therapy strategy that stimulates cardiovascular repair could improve recovery for heart attack patients. Heart attacks inflict severe damage on the heart and blood vessels, tissues with limited capacity for self-repair. Researchers led by Kiwon Ban of the City University of Hong Kong and Hun-Jun Park of the Catholic University of Korea, Seoul, have now demonstrated that a gene responsible for cardiovascular development can also efficiently stimulate heart repair. They used viruses to deliver the gene into a mouse model of heart attack, and showed that treated heart tissues exhibited strong recovery relative to untreated controls. The treatment reduced scar tissue formation and promoted proliferation of the cells lining blood vessels and blood vessel formation, measurably improving heart function. This approach could lay the groundwork for treating a common potentially fatal event.</P></▼2>

우라늄 이온의 선택흡착

정종헌,김준수,오원진,유재형,유승곤 한국공업화학회 2002 응용화학 Vol.6 No.1

Uranium-containing aqueous wastes have been treated by electrosorption on a carbon electrode composed of activated carbon fibers(ACFs) in a continuous flow-through cell. Effective uranium (VI) removal is accomplished when a negative potential in the range of -0.3 to -0.9V (vs. Ag/AgCI) is applied to the carbon electrode. For a feed concentration of 350mg/l, the concentration of U(VI) in the cell effluent is reduced to less than lmg/l. Electrosorption capacity over 552 mg_uranium/g_ACF is reached.

포켓가공을 위한 오프셋 및 공구경로 연결 알고리즘

허진헌,김영일,전차수 한국경영과학회 2003 한국경영과학회 학술대회논문집 Vol.- No.1(1)

Presented in this paper is a new fast and robust algorithm generating NC tool path for 2D pockets with islands. The input shapes are composed of line segments and circular arcs. The algorithm has two steps: creation of successive offset loops and linking the loops to tool path. A modified pair-wise technique is developed in order to speed up and stabilize the offset process, and the linking algorithm is focused on minimizing tool retractions and preventing thin-wall cutting. The proposed algorithm has been implemented in C++ and some illustrative examples are presented to show the practical strength of the algorithm.

실시간 응용프로그램을 위한 최악 실행 시간 분석

元裕憲,徐鎭哲,李俊東 弘益大學校 科學技術硏究所 1999 科學技術硏究論文集 Vol.10 No.1

Worst-case execution time(WCET) is part of the timing analysis of real-time systems. The goal of timing analysis is to validate that a real-time computer system fulfills the specified temporal requirements of a given application in all predicated situation. In this paper, We illustrate a new language, Timing-C, and design a timing tool for predicting WCET in it. Timing-C is added time constraint and a few restriction in the subset of C. Using Timing-C, programmer need not learn a new language and can demonstrate program's timing activity.

LLCE법에 의한 천연산 은어의 휘발성 향기성분 분석

김훈,조우진,안준석,조두희,김지혜,차용준 창원대학교 생활과학연구소 2003 생활과학연구 Vol.- No.7

The volatile compounds in natural sweet smelt were analyzed by liquid liquid continuous extraction/gas chromatography/mass spectrometry (LLCE/GC/MS) methods. A total of 64 compounds were detected in sample, and these were mainly composed of lipid oxidative compounds such as aldehydes, ketones, alcohols, acids and hydrocarbons besides terpens and aromatic compounds. Among these, 9-carbon compounds including (E)-2-nonenal, (E,Z)-2,6-nonadienal and (E,Z)-3,6-nonadien-1-ol were suspected to have a key role on odor of natural sweet smelt because these compounds have low odor threshold and water melon-like and melon-like odor, characteristic odor in sweet smelt.

생쥐 대장 평활근 세포에서 내향 정류성 칼륨 전류의 특성 연구

이은주,김명,정명섭,조향훈,김기훈,하현철,김준수,최석,전제열 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.2

Objectives: K^(+) channels play an important role in regulating cellular excitability. The aim of this study was to know whether or not inward rectifier K^(+) channel exists in colonic smooth muscle cells. Methods: Mouse colonic smooth muscle cells were isolated using collagenase, and then we recorded their membrane currents using a whole-cell patch clamp technique. Results: With 90 mM K^(+) in bath, hyperpolarization-induced inward currents from -120 mV to 20 mV with 400 ms duration at a holding potential of -10 mV showed rapid activation, inactivation and inward rectification. The inactivation showed single exponential time course. Reduction of external K^(+) to 60 mM decreased the amplitudes of the currents in whole test voltage range and shifted the reversal potential to more negative potential. The inactivation process and peak currents of hyperpolarization-induced inward currents were not affected by removing external Na^(+). External Ba^(+) blocked hyperpolarization-induced inward currents by dose-dependent manner and pure Ba^(+)-sensitive currents showed strong inward rectification. Cs^(+) also suppressed hyper- polarization-induced inward currents. Ba^(+) and Cs^(+)-induced inhibitiOn of hyperpolarization-induced inward currents was voltage-dependent, and the extent of inhibition increasing with membrane hyperpolarization. Conclusions: These results suggest that inward rectifier KU channels may exist in proximal colonic smooth muscle and may play an important role in regulating membrane potential.

가스터어빈 연소기의 형상 변화에 따른 화염안정성에 관한 실험적 연구

김동훈,김진곤,채준희 한국 항공대학교 항공산업기술연구소 1996 航空宇宙産業技術硏究所 硏究誌 Vol.6 No.-

현대의 항공기용 가스터어빈 연소기의 설계에서 화염안정성은 가장 먼저 고려되어야 할 중요한 설계변수 중 하나이다. 연소기 형상에 대한 변화가 화염안정성에 미치는 영향을 이해하기 위해 단순화한 모델 연소기를 이용하여 실험적 연구를 수행하였다. 동일한 면적 조건에서의 연소기 입구단 공기구멍의 갯수변화와 제1차 공기구멍열과 희석 공기구멍열의 축방향 위치변화를 변수로 고려하였다. 희박 연소 한계는 육안으로 측정하였으며 연료는 프로판을 사용하였다. 입구에서 제1차 공기구멍열까지의 거리 증가와 입구단 공기구멍의 갯수 감소로 좀 더 안정된 화염을 얻을 수 있었다. 희석 공기구명의 존재는 전체 공기구멍의 면적 증가비와 동일하게 화염안정성을 증가시켰으며 희석 공기구멍열의 위치는 화염안정성에 무관함을 알 수 있었다. In the design of modern aircraft gas turbine combustor, The flame stability has been one of major factors to be considered at the first step. To understand the effect of the variation in combustor geometry for flame stability, an experimental study was performed in a simple model combustor. A parametric variation in the number of combustor inlet air-holes at the condition of constant area and axial location of primary and dilution air-hole row was performed. Lean blow-out limit was measured by visual and propane was used as fuel. The increase in the length from inlet to primary row and decrease in the number of inlet air-holes lead to more stable flame. At the existence of dilution air-hole low, flame stability was increased the same ratio as total air-hole area. The location of dilution air-hole row was independent on flame stability.

성장기 Ⅱ급 부정교합자에서 골격 형태에 따른 액티베이터 사용 효과에 관한 연구

김준헌,이진우 대한치과교정학회 2007 대한치과교정학회지 Vol.37 No.1

본 연구는 성장기 Ⅱ급 부정교합자를 골격 형태에 따라 분류하여 액티베이터 사용 효과를 비교, 분석하고 그 결과를 진단, 치료 계획 수립 등 임상 과정에 연계시키기 위해 시행되었다. 실험은 수완부 방사선 사진상 Fishman 분류의 2, 3, 4 단계로 최대성장기 이전의 Ⅱ급 부정교합자를 대상으로 하였다. 대조군은 ANB 3도 이상이며 악정형 장치 치료를 받지 않고 치열교정 치료만 받은 환자로 총 25명(남자 15명, 여자 10명)이고 실험군은 액티베이터 치료를 받은 환자로 총 116명(남자 53명, 여자 63명)이었다. Articular angle과 gonial angle을 이용하여 골격 형태를 hyperdivergent type과 hypodivergent type으로 분류하여 액티베이터 치료효과를 비교한 결과 hypodivergent한 골격 형태를 가진 환자에서 더 큰 효과를 보였다. 따라서 성장기 Ⅱ급 부정교합자의 진단과 치료계획 수립 시 안모의 골격 형태 분류를 통해 액티베이터의 효과를 예측할 수 있다고 생각한다. Objective: To establish proper diagnosis and treatment plan for skeletal Class Ⅱ malocclusions, some important factors to consider are the patient's skeletal morphology, prognosis as well as the treatment effects. Therefore, the present study analyzed the effects of activator treatment on different skeletal patterns in growing Class Ⅱ malocclusion patients. Methods: A total of 116 patients (53 boys & 63 girls) in the experimental group were treated with the activator appliance. The experimental group was classified into either hyperdivergent or hypodivergent groups according to articular and gonial angles. Results: Patients with hypodivergent growth patterns showed good effects of activator treatment. Conclusion: It seems conceivable that through classifying adolescent Class Ⅱ malocclusion patients into different skeletal patterns, activator treatment effects may be predicted during the diagnosis and treatment planning stage.

고압 커패시터의 고장 분석을 통한 신뢰도 예측

양석준,김진우,신승우,이희진,신승훈,유동수,장석원 한국비파괴검사학회 2001 한국비파괴검사학회지 Vol.21 No.6

본 논문은 고압 커패시터의 고장분석과 신뢰성 예측 결과를 다루고 있다. 부품의 수명과 고장률을 예측하기 위해서 두 가지 방법으로 고장 모드와 고장 메커니즘을 연구하였다. 에폭시 수지로 성형된 고압 커패시터가 절연내압 시험 하에서 저항이 제로로 되는 고장에 대하여, 근본원인 고장분석 체계를 효과적으로 수립함으로써 고장 메커니즘의 원인을 분석하였다. 특히 세라믹-에폭시 계면에서의 절연파괴 고장 현상이 강조되었으며, 본 연구에서 얻어진 결과의 타당성은 마그네트론에 장착된 고압 커패시터의 열사이클 시험 수행에 의한 가속시험 결과로부터 입증되었다. 시험 결과들은 결함이 있는 로트를 신속히 규명하고 B10수명을 결정하는데 유용하게 사용할 수 있다. 또한, 유전체의 절연파괴에 대해서 부하-강도 간섭모델을 이용하여 고장률을 예측하였다. This paper presents a result of failure analysis and reliability evaluation for high voltage ceramic capacitors. The failure modes and failure mechanisms were studied in two ways in order to estimate component life and failure rate. The causes of failure mechanisms for zero resistance phenomena under withstanding voltage test in high voltage ceramic capacitors molded by epoxy resin were studied y establishing an effective root cause failure analysis. Particular emphasis was placed on breakdown phenomena at the ceramic-epoxy interface. The validity of the results in this study was confirmed by the results of accelerated testing. Thermal cycling test for high voltage ceramic capacitor mounted on a magnetron were implemented. Delamination between ceramic and epoxy, which might cause electrical short in underlying circuitry, can occur during curing or thermal cycle. The results can be conveniently used to quickly identify defective lots, determine B10 life estimation each lot at the level of inspection, and detect major changes in the vendors processes. Also, thd condition for dielectric breakdown was investigated for the estimation of failure rate with load-strength interference model.

인슐린 비의존성 당뇨병에 동반된 심상성 천포창 1 례

이상준,박진용,임태형,윤영호,권순대,남창욱,허규찬,박근용,구본식,김상표,장종억 啓明大學校 醫科大學 1997 계명의대학술지 Vol.16 No.3

저자들은 인슐린 비의존형 당뇨병 환자에서 통증성 구강내 미란 및 전신성 수포성 미란을 동반한 심상성 천포창 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Various skin conditions such as diabetic dermopathy, necrobiosis lipoidica diabeticorum, bullosis diabeticorum, granuloma annulare occur frequently in diabetes, although common lesions may be associated by chance. Pemphigus vulgaris(PV) is a blistering skin disease seen predominantly in elderly patients. This disorder is characterized by the loss of cohesion between epidermal cells with the resultant formation of intraepidermal blisters. These blisters rupture easily, leaving denuded areases that may crust and enlarge peripherally. In half or more of patients, lesions begin in the mouth: approximately 90% of patients have oromucosal involvement at some time during the course of their disease. But the relationship of this disorder with diabetes is not clear. We experienced a case of pemphigus vulgaris associated with NIDDM in 67 years old woman who was admitted due to painful oral ulcer and control of blood glucose, so we present this case with a review of literatures.