Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

비흑색종 피부암에서 고에너지 X-선 방사선치료

장지영,오윤경,천지선 朝鮮大學校 附設 醫學硏究所 2009 The Medical Journal of Chosun University Vol.33 No.S

Purpose: To evaluated the efficacy of radiotherapy in the nonmelanoma skin cancer. Materials and Methods: Eleven patients were diagnosed as non-melanoma skin cancer of the head and trunk. There were 9 women and 2 men. The mean age was 70 years with a range of 35 years to 93 years. Seven patients had squamous cell carcinoma and 4 patients had basal cell carcinoma. The mean tumor size was 3.1 cm (range 1 to 8 cm). All patients were treated with 6-megavoltage X-ray radiotherapy. Mean biological effective dose (BED₂) was 50.9 Gy/43 days (range: 37.5 to 59.4 Gy). Median follow-up period was 49 months (range: 18 to 107 months). Results: The local tumor control was 100% and median disease free survival time was 30 months. Local recurrence was noted in 3 patients and distant metastasis in 1 patient. Affecting factor in local recurrence was only radiation dose. By BED₂, median survival time was 30 months for ≥45 Gy and 12 months for <45 Gy (p=0.027). Radiotherapy related chronic complication was observed in 1 patient. Conclusions: High energy X-ray radiotherapy is an effective and safe treatment for nonmelanoma skin cancer, although the number of patients is small.

대학생의 전공에 따른 유행 동조성, 혁신성, 의복가치 차이에 관한 연구

장정임;윤지원;김수영;이규혜 한양대학교 2005 韓國 生活 科學 硏究 Vol.25 No.1

A change in economic and social environment has brought lots of changes in lifestyles and values of onsumers. This paper presents fashion conformity, innovativeness and clothing values of college students according to their academic majors. Respondents were 181 undergraduate students with diverse majors. The survey was conducted using a questionnaire designed to measure fashion conformity, innvativeness and utilitarian/hedonic clothing values. Seventeen items measuring fashion conformity and innovativeness and thirty six items measuring clothing values were adopted from existing studies. Chi-square, ANOVA, and multiple comparisons of means were conducted. Students were clustered into three groups of majors: Engineering/NaturalScience/Information majors (ENI), Business/Social/Humanity (BSH) majors and HumanEcology/Art/PhysicalEducation (HAP) majors. HAP majors were more innovative than ENI majors. Significant differences were found in hedonic and utilitarian aspects of clothing values. HAP majors had higher level of novelty/fun/attraction, fashion consciousness and social appraisal sensitivity dimensions. ENI majors showed higher level of comfort seeking among clothing values. No significance was found in clothing confidence, mix and match, economy, utility and handling dimensions of clothing values across different groups of majors. Results of this study imply that the consumer group according to a major has relation to fashion conformity, innovativeness and clothing values.

Encephalomyocarditis Virus 표면항원의 단일항체생산 세포주의 크론과 이의 면역학 및 생화학적 연구(I)

윤장현,이숙영,김우제,윤지원,고광삼 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

To determine the immunological and biochemical properties of monoclonal antibody against encephalomyocardins viral surface antigen, Balb/c female mice were immuunized with the purified viral surface protein of D-variant of encephalomyocarditis virus and then fused with niyeloma cells(NS-l). Sixteen monocloiial antibody-producing hybridoma cell lines were selected from non-producing hybridoma cell lines. Among them, three hybridoma cell lines produced IgG2b and other thirteen hybridoma cell lines produced IgG1. Two hybridoma cell lines, EDH-S-23 and EDH-S-24, Produced IgG2b and showed highest neutralization titer, 236. The antigenic specificity of these monoclonal antibodies was specific to D-variant of encephalomyocarditis virus. VIoreover, the monoclonal antibody showed specifically the curring effect on diabetes mellitus of mice, which was induced by the D-variant of encephalomyocarditis virus.

상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol : 식용 식물자원으로부터 활성물질의 탐색 Development of Biologically Active Compound from Edible Plant Sources

장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

혈액투석 환자에게서 발생한 재생불량성 빈혈 1예

정지용,윤나라,강대웅,오종찬,장재현,선길홍,박경희,정종훈,김현리 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Aplastic anemia is a disease characterized by pancytopenia in the peripheral blood and hypocellular bone marrow with the fatty infiltration. The causes of the acquired aplastic anemia were usually related to drugs and immune-related diseases. A 34-years old man was admitted due to general weakness. He has been going hemodialysis for 2 years because of end stage renal disease (ESRD) due to chronic glomerulonephritis. At admission, laboratory tests showed hemoglobin 6.2 g/dL, hematocrit 18.0 %, WBC 5,710mm (neutrophil 71.8%, lymphocyte 19.1%, monocyte 5.9%), reticulocyte 1.9%, platelet 93,000/mm, Fe 12.8 ug/dL, TIBC 204 ug/dL, ferritin 941.47 ng /ml, haptoglobin 0.72 g/L, vitamin B12 508.17 pmol/L, folate 24 ng/mL, total protein 6.54 g/dL, albumin 3.76 g/dL, Alk. pohsphatase 79 IU/L, AST 30.0 IU/L, ALT 39.7 IU/L, total bilirubin 0.57 mg/dl, BUN 79.4 mg/dl and creatinine 10.18 mg/dl. Peripheral blood smear showed the normocytic normochromic anemia with anisocytosis. Anemia was diagnosed but it was irresponsive to the recombinant human erythropoietin (EPO) and blood transfusion. In the bone marrow aspiration smear were there the hypoplastic myeloid and erythroid precursors. The bone marrow biopsy section showed the hypoplasia of all components (10-20%) and the fatty infiltration. We have experienced one case of the idiopathic aplastic anemia in a patient going through hemodialysis and we just report it with documentary records. 저자들은 혈액 투석 중인 환자에서 약물, 면역계 이상등의 원인을 발견할 수 없었음에도 재생 불량성 빈혈이 발생한 1예를 경험하여 문헌 고찰과 함께 보고하는 바이다.

방사선치료를 받은 후두암 환자의 특성 및 치료 결과

오윤경,장지영,도남용 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Pmpose: To present the characteristics and treatment outcome of laryngeal cancer patients who were treated by radiation therapy with or without surgery and chemotherapy. Materials and Melhods: A total of 23 patients with laryngeal cancer treated from 1998 through 2003 were available for analysis. The primary site of the cancer was the glottis in 12 patients, the supraglottis in 10 patients, and the subglottis in 1 patient. The stages were Ⅰ in 7 patients (30%), Ⅱ in 5(22%), Ⅲ in 3(13%), IV in 6(26%), and recurrent in 2(9%). The minimum follow-up period in surviving patients is 3 years after the radiation therapy. They were treated by radiation therapy at least 44 Gy with or without surgery and chemotherapy. Results: The overall survival at 1, 2, 3, 5 years in total patients were 95.6%, 82.6%, 78.2%, 73.9%. The overall survival at 1, 2, 3, 5 years in the glottic cancer patients were 100%, 100%, 91.6%, 91.6%. The overall survival at 1, 2, 3, 5 years in the supraglottic cancer patients were 90%, 70%, 70%, 60%. The 3-year overall survival showed the significant difference according to the primary site (glottic vs. supraglottic), cervical lymph node status (negative vs. positive), and overall time of radiation therapy (<50 days vs. 50-59 days vs. ≥60 days), Conclusion: The overall survival rate of laryngeal cancer patients in this analysis seems to be similar in early glottic cancers and relatively good in supraglottic cancers as compared with the results of other studies even though the total number of patients is small.

요양병원 입원환자 평가도구의 타당도와 신뢰도

이지윤,윤주영,장사랑 노인간호학회 2008 노인간호학회지 Vol.10 No.1

Purpose: The purpose of this study was to evaluate the validity and reliability of a patient assessment instrument for patients in long-term care hospitals. Method: The participants in this study were 90 patients admitted to 3 long-term care hospitals. The data were collected in May and June 2007. Criterion related validity of the K-ADL (Korean Activities of Daily Living) was tested using Spearman correlation with the K-MBI (Korean Modified Barthel Index). Inter-rater reliability between two nurses was tested using weighted kappa and agreement percentage. Results: Criterion related validity of the K-ADL with the K-MBI was good with average Spearman correlation coefficient =0.96. Correlation coefficients of items were between 0.63 and 0.96. Inter-rater reliability was satisfactory with the average weighted kappa equal to 0.76. Items with weighted kappa of more than 0.4 numbered 122 (86.5%) and of more than 0.75, 74 (52.5%) Conclusion: The patient assessment instrument for patients in long-term care hospitals is reliable and K-ADL is valid for classifying these patients based on resource utilization, and for quality monitoring indicators.

의예과 학생들의 성격유형검사(MBTI)의 특성

오윤경,장진영,박상학,류소연 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Pmpose: To investigate the characteristics of Myers-Briggs Type Indicator (MBTI) in premedical students of one medical college and to compare the personality profiles of them with those of other studies which involved the K-orean university students and other country population. Mateiials and Methods: The MBTI was used to measure the personality profiles of premedical students at the Center for Teaching & Leaming of Chosun University. One hundred and twenty five premedical students participated in this study and the MBTI profiles of them were compared to those of K-orean university students (1,441 persons), i.e. their potential patients and those of the United Kingdom (UK.) Population (1,634 persons) and a sample of UK- doctors (313 persons) of one medical college involved in other studies. Results: The premedical students had a preference for Introversion (62%) rather than Extraversion (38%), Sensing (70%) rather than Intuition (30%), Thinking (66%) rather than Feeling (34%), and Judging (57%) rather than Perceiving (43%). There were more Thinking (66% vs 57%, p=0.071) in the premedical students than in K-orean university students with borderline significance. There were significantly more Thinking (66% vs 46%, P=0.000) and more Introversion (62% vs 48%, p=0.002) than in UK- adult population. Also there were significantly more Sensing (62% vs 48%, p=0.002), significantly less Judging (57% vs 68%, P=0.034), and more Introversion (62% vs 53%, 0=0.094) with borderline significance than in UK doctors. But no difference was shown in Thinking between the premedical students and UK- doctors, Conclusion: The personality profiles of the premedical students in this study differed in Thinking with borderline significance from K-orean university students and significantly differed from the UK adult population in Thinking and Introversion showing the cultural difference. No difference of Thinking between the premedical students and UK doctors despite of cultural difference suggests the correlation between the Thinking and medical professional choice.