RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

태안해안국립공원 학암포 지역의 조류 현황

강화연(Hwa-Yeon Kang),서슬기(Seul-Gi Seo),박세영(Se-Yeong Park),이지용(Ji-Yong Lee),권영수(Young-Soo Kwon) 국립공원연구원 2015 국립공원연구지 Vol.6 No.4

서해안 육상지역을 통과하는 조류의 이동생태를 파악하기 위해, 2014년 7월부터 2015년 6월까지 태안해안국립공원 학암포 지역의 조류상을 조사하였다. 조사 결과 관찰된 조류는 총 14목 30과 88종 23,230개체이며, 평균 조사일당 159.7개체가 관찰되었다. 전체 우점종은 괭이갈매기 Larus crassirostris 21.1%, 되새 Fringilla montifringilla 18.5%, 박새 Parus major 12.7%, 붉은머리오목눈이 Paradoxornis webbianus 8.2%, 노랑턱멧새 Emberiza elegans 5.8% 순이었다. 이동시기인 5월과 10월에는 각각 56종, 조사일당 평균 310.5개체로 가장 많은 종과 개체수가 기록되었다. 종과 개체수가 가장 적은 시기는 여름철인 8월로, 기록된 종 수는 24종이며 조사일당 평균 95개체가 관찰되었다. 종풍부도(R ) 지수는 가장 많은 종이 관찰된 5월에 가장 높았으나, 종다양도(H )와 종균등도(E )는 오히려 이동시기에 낮은 편으로 나타났으며, 이는 일부 상위 우점종의 개체수가 월등히 증가했기 때문인 것으로 판단된다. 환경부, 문화재청, IUCN 등이 지정한 멸종위기 및 법정보호종은 총 9종이 관찰되었다. From July 2014 to Jun 2015, bird survey was conducted in Hakampo, Taeanhaean National Park. A total of 23,230 and 159.7 per day of birds representing 14 orders, 30 families, 88 species were observed. The most abundant species were the Black-tailed Gull Larus crassirostris (21.1%), the Brambling Fringilla montifringilla (18.5%), the Great Tit Parus major (12.7%), the Vinous-throated Parrotbill Paradoxornis webbianus (8.2%), Yellow-throated Bunting Emberiza elegans (5.8%). The number of species and individual was the highest in May and October, respectively, as 56 species and 310.5 individual per day were recorded. According to Korean Ministry of Environment, Cultural Herigage Adinistration of Korea and IUCN Red List, a total of 9 protected species were recorded during the survey.

C-reactive protein accelerates DRP1-mediated mitochondrial fission by modulating ERK1/2-YAP signaling in cardiomyocytes

( Suyeon Jin ),( Chan Joo Lee ),( Gibbeum Lim ),( Sungha Park ),( Sang-hak Lee ),( Ji Hyung Chung ),( Jaewon Oh ),( Seok-min Kang ) 생화학분자생물학회 2023 BMB Reports Vol.56 No.12

C-reactive protein (CRP) is an inflammatory marker and risk factor for atherosclerosis and cardiovascular diseases. However, the mechanism through which CRP induces myocardial damage remains unclear. This study aimed to determine how CRP damages cardiomyocytes via the change of mitochondrial dynamics and whether survivin, an anti-apoptotic protein, exerts a cardioprotective effect in this process. We treated H9c2 cardiomyocytes with CRP and found increased intracellular ROS production and shortened mitochondrial length. CRP treatment phosphorylated ERK1/2 and promoted increased expression, phosphorylation, and translocation of DRP1, a mitochondrial fission-related protein, from the cytoplasm to the mitochondria. The expression of mitophagy proteins PINK1 and PARK2 was also increased by CRP. YAP, a transcriptional regulator of PINK1 and PARK2, was also increased by CRP. Knockdown of YAP prevented CRP-induced increases in DRP1, PINK1, and PARK2. Furthermore, CRP-induced changes in the expression of DRP1 and increases in YAP, PINK1, and PARK2 were inhibited by ERK1/2 inhibition, suggesting that ERK1/2 signaling is involved in CRP-induced mitochondrial fission. We treated H9c2 cardiomyocytes with a recombinant TAT-survivin protein before CRP treatment, which reduced CRP-induced ROS accumulation and reduced mitochondrial fission. CRP-induced activation of ERK1/2 and increases in the expression and activity of YAP and its downstream mitochondrial proteins were inhibited by TAT-survivin. This study shows that mitochondrial fission occurs during CRPinduced cardiomyocyte damage and that the ERK1/2-YAP axis is involved in this process, and identifies that survivin alters these mechanisms to prevent CRP-induced mitochondrial damage. [BMB Reports 2023; 56(12): 663-668]

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

北方政策에 관한 國民意識調査

康智漢,嚴載鎬,朴仁熙 경북대학교 평화문제연구소 1990 평화연구 Vol.15 No.-

낙동강 물 관개논의 농업환경 특성과 질소, 인산 요구량

강위금,이재생,고지연,박창영,정기열 한국환경농학회 2004 한국환경농학회지 Vol.23 No.3

낙동강 물을 관개하여 벼를 재배하는 강 상류의 안동 풍산에서부터 하류의 부산 구포까지 7개 지역 논토양의 농업환경 특성을 1999년부터 2년간 비교 분석하였다. 토양의 중금속 함량은 자연함량 안팎이었으며 가장 높았던 곳은 부산 강서 지역이었고 다음으로는 고령 성산 지역이었다. 그러나 현미중의 중금속은 자연함량 이하를 보였다. 토양의 화학성은 고령 성산지역과 부산 강서지역에서 높았는데, 1999~2000년도의 영농 후 토양에서 고령은 pH (5.9~6.1), EC(0.8~0.9 dS/m), 유효인산(155~201 mg/kg), 치환성 칼슘(6.7~7.4 cmol^(+)//kg), 치환성 마그네슘(1.92~2.50 cmol^(+)/kg), 치환성 칼리(0.18~0.21 cmol^(+)//kg )가 가장 높았고 부산에서는 유기물(23.0~29.1 g/kg), 총질소(1.6~l.8 mg/kg)가 가장 높았다. 낙동강 물의 관개수질과 토양화학성을 고러한 벼의 진단시비량은 질소의 경우 110 kg/ha를 기준으로 안동 21.4%, 상주 11.85, 구미지역에서 8.8% 정도 증시할 필요가 있었으나, 고령에서는 14.9%, 창녕은 4.6%, 밀양 하남은 4.55, 부산 강서지역은 11.5% 내외로 줄일 수 있을 것으로 분석되었다. 그리고 인산질 비료는 45 kg/ha 기준으로 창녕 도천에서만 18.9% 줄여 주고 다른 지역에서는 기본시비량인 30 kg/ha만 시용하여도 무방할 것으로 판단되었다. 논토양에 분포하는 세균과 사상균, 방선균, 중온성 Bacillus, 형광성 Pseudomonas, Biomass C는 유기물과 총 질소, 인산 등의 양분함유량이 많았던 고령 성산과 그 하류지역에서 높았다. Agroenvironmental characteristics in paddy fields irrigated with the water of Nagdong river were analyzed along the river watershed for two years from 1999. The sites monitored from upper reaches of the river were Andong, Sangju, Gumi, Goryeong, Changnyeong, Milyang and Pusan. In paddy soils, the contents of heavy metals such as Cd, Cr, Cu, Pb, Ni, Zn and As were around natural values showing the highest values in Pusan followed by Goryeong. In brown rice, the contents of heavy metals were lower than natural values. Soil chemical properties appeared higher values in the lower reaches including Goryeong than the upper ones. The highest parameters in Goryeong were pH (5.9~6.1), EC (0.8~0.9 dS/m), Av.P₂O_(5) (155~201 mg/kg), exchangeable Ca (6.7~7.4 cmol^(+)/kg), Mg (1.92~2.50 cmol^(+)/kg), K (0.18~0.21 cmol^(+)/kg) and those in Pusan were organic matter (23.0~29.1 g/kg) and T-N (1.6~1.8 mg/kg). In conclusion, the recommended rates of N fertilizer for rice cropping were 21.4%, 11.8% and 8.8% high for Andong, Sangju and Gumi, respectively and 14.9%, 4.6%, 4.5% and 11.5% low for Goryeong, Changnyeong, Milyang and Pusan, respectively reflecting the chemical properties of soils and the quality of irrigation water on the basis of 110 kgN/ha. In the case of phosphorous, the rates were 18.9% and 33.3% low for Changnyeong and others, respectively on the basis of 45 kgP₂O_(5)/ha. The populations of bacteria, fungi, actinomycetes, Bacillus, fluorescent Pseudomonas and Biomass C were high at the lower reaches including Goryeong, which showed relatively much nutrient contents of organic matter, total N and phosphorous etc..

Apolipoprotein E 다형성에 따른 사업장 근로자의 혈중 지질농도, 영양소 섭취 및 건강관련 생활습관

박유경,조상운,강지연,백윤미,성숙희,최태인 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.5

The purpose of this study was to investigate the association among nutrient intakes and health-related lifestyles with cardiovascular disease risk assessed by blood lipid profile according to Apolipoprotein E genotypes. Middle-aged industrial male workers who had completed their annual medical examination were recruited and data of 675 subjects who finished the nutrient survey were used in the analysis. Anthropometric parameters, dietary assessment (FFQ), health-related lifestyles and blood profiles were used for statistical analyses. Apo E genotype groups were classified into the following three genotypes: Apo E2 group (including E2/E2, E2/E3, E2/E4), Apo E3 group (including E3/E3), Apo E4 group (including E3/E4, E4/E4). The frequency of Apo E2, E3, and E4 allele were 13.3%, 75.0% and 11.7% respectively. There were no significant differences in the anthropometric parameters depending on different Apo E genotypes. Also, no significant differences in the nutrient intakes were found according to the genotype groups. The nutrient intakes of all subjects were similar to or higher than the level of KDRIs (Dietary Reference Intakes For Koreans) except for intakes of calcium (67.44% of KDRIs), vitamin A (73.83% of KDRIs) and vitamin B2 (78.02% of KDRIs). Also, there were no significant differences of health-related lifestyles according to Apo E genotype groups. As for the lipid profiles, Apo E4 group had significantly higher total and LDL-cholesterol concentrations than the Apo E2 group (p < 0.05). We confirmed that plasma total and LDL-cholesterol concentrations were greatly influenced by Apo E genotypes. However, nutrient intakes and health-related lifestyles were not associated with Apo E genotypes.

벌 자상에 의해 발생한 횡문근융해증과 급성신부전

박상곤,김영대,강지은,신정현,신병철,박차영,정춘해 朝鮮大學校 附設 醫學硏究所 2003 The Medical Journal of Chosun University Vol.28 No.2

Rhabdomyolysis is a syndrome characterized by muscle necrosis and release of intracellular muscle constituents into the circulation. The spectrum of causes is quite broad and included disorders of traumatic, metabolic, infectious and toxic origin. However, rhabdomvolvsis and acute renal failure following bee sting is rare and its pathogenesis is not well known. We experienced a case of 63-year-old man with acute renal failure and rhabdomyolysis caused by bee sting. He was stung by bee on scalp and neck. Oliguria was developed within several hours. He has fully recovered after general supportive care including fluid therapy and diuretics. This case demonstrates that rhabdomvolysis with consequent acute renal failure are able to developed by bee sting.

수행 평가 도구를 이용한 심화·보충형 수준별 교수 전략 개발 : 과학 3의 물질의 구성 Composition of Matter Section in Science 3

강순희,김지영,박은미,이윤하 이화여자대학교 사범대학 교과교육연구소 2005 교과교육학연구 Vol.9 No.2

제7차 교육 과정의 과학 교과에 대한 심화·보충형 수준별 수업 전략을 개발하여 현장 과학 교사들에게 구체적인 도움을 주고자 하는 것이 본 연구의 목적이다. 수업 운영 형태는 동일 학급 내 교과 수준별 분단 편성이며, 연구된 구체적인 단원은 중학교 과학 3의 ‘물질의 구성’ 단원으로 총 17차시가 소요된다. 먼저 현실적으로 중등 과학 교육 현장에서 실현 가능한 과학과 수준별 교육 과정에 관련된 이론적 배경을 고찰하였다. 둘째, ‘물질의 구성’ 단원에 대하여 차시 수업의 목표로 사용 가능한 구체적인 성취 기준을 지식, 탐구, 태도 영역으로 개발하였다. 셋째, 앞에서 개발한 성취 수준의 도달 정도를 판별하는 평가 기준을 개발하고, 이 성취 수준과 평가 기준을 토대로 하여 동일 학급 내 과학 교과 수준별 분단 편성을 위한 진단 평가 도구들을 개발하였다. 넷째, 이 진단 평가 도구에 의하여 학생들을 수준별 소집단 분단으로 분류하는 구체적인 방법을 제시하였다. 마지막으로, 각 동일 학급 내 수준별 분단에 사용할 수 있는 보충 활동지를 개발하였으며 사용하는 방법을 제시하였다. The purpose of this study is to development of instructional strategy for differentiated curricular by enrichment and supplementary instructions about junior high school science under the 7th National Curricular Revision. The ability grouping for differentiated curricular in this study was within class ability grouping in same year. The composition of matter in junior high school science 3 was selected for this study and analyzed to develope the detailed achievement standards and assessment standards of school class level for science teacher. Based on those detailed achievement standards and assessment standards of school class level, the concept clusters of the composition of matter section was classified. The diagnostic evaluation which was the performance assessment tools according to the concept clusters was developed to classify for enrichment and supplementary groups. Also the method of classification about enrichment and supplementary groups in science class was suggested in details. Finally, teaching and learning materials for supplementary groups according to the concept clusters were developed about the composition of matter section in science 3 at junior high school.

재발된 원발성 고환림프종이 동반된 류마티스 관절염 환자에서 Rituximab으로 치료한 1예

박열,박상민,한경택,강지인,이준,김상현 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Several studies have shown that patients with rheumatoid arthritis(RA) have a doubled to tripled increased risk for developing lymphomas compared with the general population, Indeed, primary testicular lymphoma accounts for about 1% of all lymphomas. We present a case of RA with primary testicular lymphoma treated with rituximab. A 67-year-o1d man was admitted due to aggravated polyarthralgia and morning stiffness. hIe was diagnosed testicular diffuse large B cell lymphoma (DLBCL) and done orchidectomy last year. There was no adjuvant chemotherapy nor radiotherapy because of patient's loss. Abdominal computed tomography (CT)scan showed about 6x4cm mass on the left adrenal gland. Malignant lymphoma (DLBCL) was proven by CT guided needle biopsy and he was treated with CHOP (cyclophosphamide, hydroxydaunorubicine, oncovin, prednisone) and rituximab (375㎎/㎡) combination regimen. After two cycle treatment, the clinical symptoms and laboratory data improved.