당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

만성 요통 환자들에서 요추부 저항 운동을 포함한요통 운동의 효과

이강우,권정이,김현숙,이병섭,강재영,박원하 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.3

컴퓨터 프로그램을 이용한 과학실험 자료해석

강동식,강정우 濟州大學校 師範大學 科學敎育硏究所 1997 科學敎育 Vol.14 No.-

The appropriate uses of the computer programs in science tend to quick looks at relatively simple or idealized situations involving limited data. All students check the calculations done by any software package before using it to analyze their data

脂質 종류에 따른 나일틸라피아의 성장 효과

강석중,최병대,정우건 國立統營水産專門大學 附設 水産科學硏究所 1994 수산과학연구소보고 Vol.5 No.-

양어 사료에 피드오일을 첨가하면 사료 효율이 향상된다. 나일틸라피아가 요구하는 필수 지방산의 종류를 알아보기 위하여 대두유 및 옥수수유 등의 식물유 및 오징어 간유, 어유를 사료에 첨가하고 사육하여 다음과 같은 결과를 얻었다. n-6 계열이 풍부한 식물유를 투여한 실험구가 n-3 계열의 어유를 투여한 실험구 보다 성장이 좋았으며 그 중에서도 대두유 첨가구가 가장 양호하였다. 총 지질중의 20:3n-9의 수준은 유지의 종류에 따라 현저한 차이는 없었으나 극성 지질중의 20:3n-9의 수준은 어유 및 오징어 간유 보다는 식물유구에서 낮게 나타났다. 틸라피아 사료에 첨가하는 지질로서는 n-6계열을 많이 포함하는 것이 적당할 것으로 나타났다. Experiments were conducted to find out suitable dietary lipids in the practical feed for Nile tilapia, Oreochromis niloticus. Soybean oil, corn oil, fish oil or cuttlefish liver oil was mixed in the diet and tested. The best weight gain and feed efficiency were obtained from the fish fed soybean oil or corn oil, both rich in 18:2n-6. But the growth rate of fish fed diet with fish oil or cuttlefish liver oil were inferior. The levels of 20:3n-9 in the total lipid were not different in all tested lipids, but that in the polar lipid of the fish oil or cuttlefish liver oil were lower than that in the soybean oil or corn oil. Thus, vegetable oil such as soybean oil or corn oil, rich in 18:2n-6, were suitable as dietary lipid for Nile tilapia. Unlike other fish species, fish oil or cuttlefish liver oil showed no essential dietary value for this species.

2차원 Dilaton-중력 이론의 표준 양자화

강동식,강정우,박규은 濟州大學校 師範大學 科學敎育硏究所 1994 科學敎育 Vol.11 No.1

The quantization of the 2-dimensional Dilaton-Gravity theory on a compact spatial section is carried out in a canonical method. The wave function is obtained for a homogeneous & isotropic universe.

학교주변 소음실태의 비교조사 연구 : 제주도 초·중학교를 중심으로

강종철,윤지홍,강정우 濟州大學校 師範大學 科學敎育硏究所 1985 科學敎育 Vol.2 No.-

The conditions of noise pollution around the elementary schods, the middle schools, and the high schools near the highway around the Cheju-do and those in cheju city are investigated. Thirteen percents of schools near the highway around the Cheju-do suffer noise pollution. So do sixty percents of schools in Cheju city. The causes of noise are due to the location of schools near the road and the bus stops. klaxon and overspeed of cars. and insufficient measure for the noise control.

과학사적 학습지도에 의한 중학생들의 낙하운동 개념 이해

강정우,강동식,김홍중 濟州大學校 師範大學 科學敎育硏究所 1997 科學敎育 Vol.14 No.-

In order to know whether in understanding concepts of the falling motion of the middle school students the learning guidance based on the history of science is the effective learning method or not, on the basis of investigation and analysis of the degree of preconception of the falling motion of students, the conceptual lesson is done through learning the process of changes of the scientific concepts appearing in the history of science and discussion. In the light of the student' motivation and interest, when lesson was over, they said that the learning guidance based on the history of science was better than the traditional ones. Students' grades were about 15% better than that of the control group.

보조학습도구로서 Mathematica를 이용한 학습모형 개발

강동식,강정우 제주대학교 1994 논문집 Vol.38 No.-

보조학습도구로서 컴퓨터는 매우 유용한 도구이다. 학생들은 문제의 중간과정에 많은 노력을 투자함으로서 문제분석이나 결과해석에 치중하지 못하는 경향이 있다. Mathematica를 이용하여 그런 노력을 완화시킬 수 있으며, 보다 많은 경우에까지 응용할 수 있어 물리개념을 확고하게 정립할 수 있다. 수업모델로써 세가지 예, 즉 Ohm의 법칙, 감쇠진동, 파의 중첩들을 제시하였다. As the assistant learning instruments, computer is a very usefoul tool. Students make great efforts in processing intermediate mathematical steps in solving the Physical probelem. Mathematica alleviates such efforts and students can make a further motice to the analysis of problems and to the interpretation of results. We propose the three learning models utilizing Mathematica as the assistant learning instrument:the Ohm's the damped oscillation, the superposition of waves.