Adipose sirtuin 6 drives macrophage polarization toward M2 through IL-4 production and maintains systemic insulin sensitivity in mice and humans

Song, Mi-Young,Kim, Sang Hoon,Ryoo, Ga-Hee,Kim, Mi-Kyung,Cha, Hye-Na,Park, So-Young,Hwang, Hong Pil,Yu, Hee Chul,Bae, Eun Ju,Park, Byung-Hyun Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.5

<▼1><P>Adipose tissue inflammation is a reproducible feature of obesity and obesity-linked insulin resistance. Although sirtuin 6 (Sirt6) deficiency has previously been implicated in diet-induced obesity and systemic insulin resistance, the adipocyte-specific role of Sirt6 in the regulation of adipose tissue inflammation and systemic metabolic dysfunction in mice fed normal chow and in humans remains elusive. Here, using <I>Adipoq-Cre</I>-mediated adipocyte-specific Sirt6 knockout (aS6KO) mice, we explored whether adipocyte Sirt6 inhibits adipose tissue inflammation and its underlying mechanism. aS6KO mice fed normal chow gained more body weight and fat mass than wild-type mice and exhibited glucose intolerance and systemic insulin resistance. Measurement of plasma and tissue cytokines and flow cytometric analysis of adipose stromal vascular cells indicated a decrease in alternatively activated M2 macrophages in the adipose tissue of aS6KO mice. Mechanistically, Sirt6 regulated the expression of the canonical type 2 cytokine IL-4 by adipocytes in a cell autonomous manner, which in turn affects M2 macrophage polarization. Consistent with animal experimental data, the degree of obesity and insulin resistance demonstrated by the body mass index, fasting blood glucose and HbA1c correlated negatively with the expression of Sirt6 in human visceral fat tissues. Collectively, these results suggest that adipocyte Sirt6 regulates body weight gain and insulin sensitivity independent of diet, and the increased IL-4 production by Sirt6 and resultant M2 polarization of adipose tissue macrophages may attenuate proinflammatory responses in adipose tissue.</P></▼1><▼2><P><B>Obesity and diabetes: A control protein in fat</B></P><P>A protein in adipose tissue (composed of fat cells) helps protect against inflammation and the development of resistance to insulin that develops in obesity and can lead to type 2 diabetes. Researchers in South Korea, led by Eun Ju Bae at Woosuk University, Wanju, and Byung-Hyun Park at Chonbuk National University, Jeonju, investigated the role of Sirt6 in mice and in human adipose tissue. Deleting the mouse gene that codes for Sirt6 in adipocytes promoted the impaired response to insulin and associated increase in blood glucose levels that are two key aspects of diabetes. Changes in biochemical signaling pathways controlling immune cells called macrophages were implicated in these effects and suggest an anti-inflammatory role for Sirt6. Analysis of human adipose tissue supported these findings. The research will help understand how obesity promotes type 2 diabetes.</P></▼2>

국부 구조 분석과 장면 적응 사전을 이용한 초고해상도 알고리즘

최익현(Ik Hyun Choi),임경원(Kyoung Won Lim),송병철(Byung Cheol Song) 대한전자공학회 2013 전자공학회논문지 Vol.50 No.4

LC-MS/MS를 이용한 S.D. Rat 혈장 중 Arctiin 분석법 개발

송병정,채정우,백현문,권광일 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-

KIOM-MA128 is a novel oriental herbal medicine which is for atopic dermatitis and asthma. The purpose of this study was to develop on analytical method of arctiin in rat plasma after oral administration of KIOM-MA128. Analyte was separated on a Atlantis dC18 reverse phase column, using gradient mobile phase (A:B = acetonitrile: 0.1% formic acid in water) at a flow rate of 0.3 mL/min. Detection was performed by electrospray positive ionization mass spectrometry using multiple reaction monitoring of the transitions of arctiin at m/z 552.4 → 372.8 and internal standard (carbamazepine) at m/z 237.0 → 194.5. The limit of quantification was 1 ng/mL for arctiin. The precisions were lower than 15% and the accuracy was between – 12.21 and 3.2%. The maximum concentration found in plasma samples was 4.1 ng/mL. The present method was successfully developed for detecting arctiin in plasma and this results would be utilized to the further study.

노인 복합주거 단지의 외부공간에 관한 연구

宋秉夏,黃智鉉 홍익대학교 산업기술연구소 2002 産業技術 Vol.12 No.-

As the proportion of older persons in the Korean population begins to increase, new kinds housing designed specifically for those are becoming familiar and common (generally being called 'silver towns'). One of the reasons explaining this event is that more older persons than in previous years are now living, and also planning, independently, away from and not counting supports from their children. Unlike nursing homes, congregate housing and/or assisted living facilities offer independent living for the elderly. In order to live on their own, opportunities for meeting others and socializing become increasingly important aspects for the elderly, and pleasant outdoor spaces take a major role in supporting active activities such as exercising, gardening, and walking, as well as sitting and viewing. This study examines the necessities that should be considered early in the planning (i,e, programing) and design process of outdoor spaces for the elderly, so as to provide comfort, safety and security, and ease of access to the outdoors. Further, the case study is utilized to compare the guidelines from the literatures with the actual instances in an assisted living facility.

Mongolian gerbil 심장동맥의 해부학적 연구

송치원,정주영,이미영,김무강,현병화 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

The arterial supply to heart of mongolian gerbil has been described. Observations were made by the specimens injected with latex after the flowing through in left ventricle. The results were summaried as follows; 1. A. coronaria arose from the initial portion of Aorta in heart and A. coronaria dextra and sinistus arose in Aortic sinus on 10 specimens. A. coronaria sinistra arose more near boundary of Aortic sinus than A. coronaria dextra. 2. A. coronaria sinistra which arose from Aortic sinus divided into Ramus interventricularis sinistra and Ramus circumflexus sinistra. A. coronaria dextra which arose from Aortic sinus only runed continously as Ramus interventriculares dextra. 3. Ramus interventricular sinistra not reach the heart apex through the sulcus interventricula but stopped in thoracocostal plan extending to under part. 4. R. circumflexus runed theough sulcus coronarius, thereafter it runed from left ventricle to Sulcus interventricularis dorsalis. 5. A. coronaria dextra runed to diaphargm side through sulcus interventricula dorsi thereafter it became Ramus interventricular dextra. It distrubuted left and right ventricle wall on 8 specimens and 2 specimen stopped margo obtusus.

백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.

TMS320C54X DSP 보드를 이용한 IDEA의 구현

송종관,윤병우,류대현 경성대학교 생산기술연구소 1997 생산기술연구지 Vol.4 No.-

폐타이어 담체를 이용한 유동상 생물막공정의 고도처리 효율에 관한 연구

송창열,이택순,서규태,문병현 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

Organic and nutrient removal was investigated in a full scale fluidized phase biofilm process of 800m-(3)/day using waste tire media for domestic wastewater treatment. Various internal reciculation ratio was set as an operation condition to enhance nitrogen removal efficiency. The process operation results are as follows. Significantly high removal efficiency above 94% could be obtained by the biofilm process in terms of organics(BOD&COD_(Mn)) and suspended solid(SS). Relatively low nitrogen removal of 53% was observed at the internal recycling 100% of influent flow rate. However the removal efficiency could be increased to 70% at 200% internal recycling. The process showed also considerably high T-P removal efficiency of 80% producing effluent concentration of 0.68mg/L for 3.38mg/L influent.

골형태분석법과 micro-CT를 이용한 골소주 특성에 대한 연구

송영한,이완,이창진,지정현,이병도 대한구강악안면방사선학회 2007 Imaging Science in Dentistry Vol.36 No.1

Purpose : The research was done to investigate the effectiveness of 2D bony morphometry and microstructure of micro-computed tomography (micro-CT) on the osteoporotic bony change. We performed the bone morphometric analysis of proximal femur in ovariectomized rabbits with BMD and micro-CT examination. Materials and Methods : Twenty-one female (Newzealand, about 16 weeks old, 2.9-3.4 kg) rabbits were used. Three rabbits were sacrificed on the day when experiment began (Baseline). The remaining 18 rabbits were divided into two groups. One group was ovariectomized bilaterally (OVX) and the other animals were subjected to sham operation (Sham). Bone specimens were obtained from the right and left femur of sacrificed rabbits. At intervals of 1, 2, 3, 5, 6 months respectively, BMD tests were performed on the proximal femur by using PIXImus 2 (GE Lunar Co. USA), 2-dimensional bone morphometric analysis by custome computer program and 2D/3D bone structure analysis by micro-CT (Skyscan1072, Antwerpen, Belgium). Statistical analysis was carried out for the correlation between bone morphometry , micro-CT and BMD Result : BV/TV, Tb.Th, Tb.N of micro-CT parameters showed higher values in sham group than OVX group. N.Nd/Ar.RI, N.NdNd, N.NdTm, N. TmTm, PmB/Ar.RI, 3-D BoxSlope of 2D morphometric parameters showed higher values in Sham group than OVX group. The micro-CT parameters of Tb.Sp, Tb.N were statistically significant correlated with BMD respectively. Several 2D morphometric parameters were statistically significant correlated with BMD respectively. Conclusion : Several parameters of 2D bony morphometry and micro-CT showed effective aspects on the osteoporotic bony change. (Korean J Oral Maxillofac Radiol 2007; 37 : 27-33)

심장사상충증 이환견의 치료 1례

심재현,김유수,김건우,이병희,김지용,박성준,송근호 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 4-years old pointer dog was referred to the Veterinary Teaching Hospital of Chungnam National University with chief compliment of anorexia, exercise intolerance, hemoptysis, coughing and ascites. It was diagnosed as heartworm disease (class 3) by physical and laboratory examination, radiography, ultrasonography and electrocardiography. This dog was undertaken by adulticidal therapy. Normal conditions and good appetite after therapy were observed.