http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
황련해독탕 및 귀비탕의 발효 전후 혈소판 응집 억제 효과에 대한 비교 연구
송병정,이수진,권광일 충남대학교 약학대학 의약품개발연구소 2012 藥學論文集 Vol.27 No.-
Recently interest of fermented oriental medicines is increasing for the purpose of disease prevention. Hwangryunghaedok-tang (HR) and Guibi-tang (GB) are widely used oriental medicines in Korea. HR and GB are well known for anti-inflammatory and anti-stress effect. Glycosides in herbal medicines are transformed to aglycone via fermentation. The fermented medicines are absorbed more and have less individual variation. In this study, HR and GB were compared before and after fermentation by anti-platelet aggregation effect. HR and GB were fermented by various lactobacillus species. The anti-platelet aggregation effect of HR and GB were measured using aggregometer by optical method. Collagen (4 μg/mL) was used as a inducer of platelet aggregation. After measuring anti-platelet aggregation effect, we analysed the data using WinnonlinⓇ program to fit simple Emax model. Then Emax and EC50 values were estimated. In results, GB had little antiplatelet effects. Emax of 5 GB samples (127, 164, 402, 442, 3163) were estimated near zero and others (129, 144, 166, 693, 744) had high EC50 values (> 1500 μg/mL). In contrast with GB, HR had antiplatelet effects. HR which was fermented by Lactobacillus casei and Lactobacillus plantarum (HR-127,HR-144) were most effective sample. Emax and EC50 of HR-127 and HR-144 was 99.99%, 174.73 μg/mL and 100.00% 160.48 μg/mL, respectively. These results indicated that Guibi-tang was inappropriate as anti-platelet aggregation agent and Hwangryunhaedok-tang would be more effective after fermentation as anti-platelet aggregation agent, warranting further study.
LC-MS/MS를 이용한 S.D. Rat 혈장 중 Arctiin 분석법 개발
송병정,채정우,백현문,권광일 충남대학교 약학대학 의약품개발연구소 2014 藥學論文集 Vol.29 No.-
KIOM-MA128 is a novel oriental herbal medicine which is for atopic dermatitis and asthma. The purpose of this study was to develop on analytical method of arctiin in rat plasma after oral administration of KIOM-MA128. Analyte was separated on a Atlantis dC18 reverse phase column, using gradient mobile phase (A:B = acetonitrile: 0.1% formic acid in water) at a flow rate of 0.3 mL/min. Detection was performed by electrospray positive ionization mass spectrometry using multiple reaction monitoring of the transitions of arctiin at m/z 552.4 → 372.8 and internal standard (carbamazepine) at m/z 237.0 → 194.5. The limit of quantification was 1 ng/mL for arctiin. The precisions were lower than 15% and the accuracy was between – 12.21 and 3.2%. The maximum concentration found in plasma samples was 4.1 ng/mL. The present method was successfully developed for detecting arctiin in plasma and this results would be utilized to the further study.
건강한 성인에서의 알코올의 집단 약물동태/약물동력에 미치는 산소의 영향 연구
송병정,권광일,백현문,황시영,채정우,윤휘열 한국임상약학회 2017 한국임상약학회지 Vol.27 No.4
Objective: To develop a population pharmacokinetics (PK)/pharmacodynamics (PD) model for alcohol in healthy volunteers and toelucidate individual characteristics to affects alcohol’s PK or PD including dissolved oxygen. Methods: Following multiple intakes oftotal 540 mL alcohol (19.42 v/v%) to healthy volunteer, blood alcohol concentration was measured using a Breathe alcohol analyser(Lion SD-400 Alcolmeter®). A sequential population PK/PD modeling was performed using NONMEM (ver 7.3). Results: Eighteenhealthy volunteer were included in the study. PK model of alcohol was well explained by one-compartment model with first-orderabsorption and Michaelis-Menten elimination kinetics. Ka, V/F, Vmax, Km is 8.1 hr-1, 73.7 L, 9.65 g/hr, 0.041 g/L, respectively. Covariate analysis revealed that gender significantly influenced Vmax (Male vs Female, 9.65 g/hr vs 7.38 g/hr). PD model oftemporary systolic blood pressure decreasing effect of alcohol was explained by biophase model with inhibitory Emax model. Ke0,Imax, E0, IC50 were 0.23 hr-1, 44.9 mmHg, 138 mmHg, 0.693 g/L, respectively. Conclusion: Model evaluation results suggested thatthis PK/PD model was robust and has good precision.
항히스타민제의 대리결과변수로서 히스타민에 의한 피내주사반응 유용성에 대한 고찰
한나영, 송병정, 백현문, 정에벤, 유영훈, 전지현, 구성우, 윤휘열, 권광일 충남대학교 약학대학 의약품개발연구소 2017 藥學論文集 Vol.32 No.-
In this study, it is reviewed that histamine-induced wheal and flare responses are potential sur-rogate endpoints for predicting the clinical effects of antihistamines in patients with allergic skin diseases. Histamine plays an important role in allergic response by inducing degranulation of mast cells due to allergen exposure and mediating the inflammatory reaction. Thus, suppression of histamine-induced wheal and flare has been noted as surrogate markers for efficacy of Hl receptor antagonists. In addition, allergy skin prick test and intradermal test using histamine have been used to diagnose the histamin-induced allergic reaction. However, it has been well known that allergic diseases are not only mediated by histamine. but also by var-ious immunological inflammatory responses. Previous studies reported that there is a lack of evidence about the correlation between antihistamines to predict clinical efficacy and antihistamine efficacy, although hista-mine-induced wheal and flare responses may be useful indicators of the dose-response relationship. In con-clusion, the evaluation for the suppression of wheal and flare after histamine injection is reasonable for de-termining the treatment of allergic simple skin diseases, but there is a limit to evaluate the efficacy in com-plex inflammatory diseases mediated IgE or T cells, or other immune complex. Therefore, further studies are needed to identify additional surrogate endpoints to predict the therapeutic effect of antihistamines in other inflammatory diseases such as allergic rhinitis, atopic conjunctivitis, allergic asthma, and so on.
유영훈,송병정,백현문,이재연,정성미,황시영,정에벤,Sudeep Pradhan,윤휘열,권광일 충남대학교 약학대학 의약품개발연구소 2016 藥學論文集 Vol.31 No.-
Getting the spotlight to traditional medicine recently, the number of adverse effect and drug-drug interaction of traditional medicine is increased. For this reason, pharmacokinetics study of traditional medicine need to scientific prove of safety and effectiveness. The method of pharmacokinetics study of traditional medicine, Botanical drug guideline of U.S. FDA recommend to measure analytical marker concentration in blood. The aim of this study is quantitative content analysis of analytical marker in citrus unshiu peel for help pharmacokinetics study of traditional medicine. We seleceted naringin, naringenin, hesperidin and hesperetin as analytical marker and used HPLC-MS/MS as analysis equipment. Internal standard (IS) is domperidone. The mass transition of hesperidin, hesperetin, naringin and naringenin were 609.4 → 300.9, 301.0 → 285.8, 579.23 → 270.9 and 271.0 → 150.8 respectively. The result of this study is that 1g citrus unshiu peel has 12.24 mg (1.224%) hesperidin, 0.66 mg (0.066%) hesperetin, 15.17 mg (1.517%) naringin, and naringenin was not detected. citrus unshiu peel analytical marker that we can detect is naringin, naringenin, hesperidin. We could confirmed amount of citrus unshiu peel analytical marker through this study and our data further support the pharmacokinetics study of traditional medicine.