한국산야생등줄쥐(Apodemus agrarius coreae)의 해마복합체에서 neuropeptide Y 면역반응세포에 관한 연구

김무강,송치원,이경열,박일권,정영길,이강이,김종섭 충남대학교 수의과대학 동물의과학연구소 1997 動物醫科學硏究誌 Vol.5 No.-

Neuropeptide Y(NPY) was first isolated from porcine brain. This discovery has lead some workers to study the distribution of this peptide in the central nervous system of various mammals. In this study the distribution pattern of neuropeptide Y-immunoreactive neurons was examined in the hippocampal complex of Striped field mouse in Korea(Apodemus agrarius coreae) Experimental animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through the left ventricle and the ascending aorta. The brains were removed and transferred 10%, 20% and 30% sucrose solution. Sections were cut on a cryostat into 40㎛-thick. The tissue was immunostained with free-floating and avidin-biotm complex(ABC) method. The results obtained in this study were summarized as followings. 1. The most of NPY-IR neurons in hippocampus proper were found in the polymorphic layer and a few was found in the pyramidal layer. 2. The process run vertically in the deep polymorphic layer and run parallel to corpus callosum in superficial polymorphic layer. 3. The shapes of neurons in hippocampus proper were varied of polymorphic, oval or round, and the nerve fibers were found in the molecular laver and the hippocampal fissure. 4. The highest density in number of NPY-IR neurons on dentate gyrus were found in hilar area of dentate gyrus. 5. The most of NPY-IR nerve fibers were found in molecular cell layer.

Nicotine이 발생중인 鷄胎의 體重 成長에 미치는 영향

金武剛,金弘善,柳時潤,申台均,李永浩,趙聲煥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

The authors studies the effects of Nicotine on the Body weight growth of the developmental chick embryo. On the sixth day of incubation, 0.41mg, 0.82mg and 1.65mg of Nicotine were injected into the chorioallantoic sac of each egg of three groups and one group was injected physiological saline solution as a control, and the eggs were broken for weighting the body weight in every other day from the twelfth day to eighteenth day of incubation. In an attempt to analyze the growth of the body weights, growth formula of Zimmerman and relative growth formula of Huxley were applied. The following results were obtained. 1. The growth of chick embryo boby weight was affected as inhibiting by the Nicotine. The inhibiting effects were in proportion to the injected Nicotine dosage. 2. The growth quantity of the body weight injected 1.65mg was almost half weight of the control group body weight. 3. Growth rate was decreased continuously with tine until 18th day in the 1.65mg injected group but it was increased in 0.41mg and 0.82mg injected group with time lapse from 12th day to 18th day, and 0.41mg injected group was more increased than 0.82mg injected group. 4. Specific growth rate decreased in the all of 0.41mg, 0.82mg and 1.65mg injected group and in the decrease degree 1.65mg and 0.82mg injected group ware severe and 0.41mg injected group was slight. 5. Growth centers of growth gradient were 12th day in the 0.41mg injected group and 18th day in the 0.82mg and 1.65mg injected groups. 6. Relative growth rate was also decreased proportionally in related with injected Nicotine dosage.

酪農民의 人獸共通寄生蟲 疾患에 關한 實態調査硏究

金武剛,金鍾煥,張大允 충남대학교 의과대학 지역사회의학연구소 1980 충남의대잡지 Vol.7 No.2

A Survey of zoonoses such as the Taeniasis and Cysticercosis were carried out among the dairy farmers in the some areas of Chung cheong nam Do. The results are summerized as follows : 1. Past history of Taenia infection rate was 23.1% by the 1584 Questionnaires (male 1038, female 546). Among of them the worm recovery rate was 5.1%. By the sex difference, the positive rates of Taeniasis showed that 26.3% in male and 17.0% in female and the recovery rates of the worm were 5.2% in male and 2.6% in female, and the increasing tendency of Taenia infection was found in the above 20 age group. 2. Both bovine and swine raw meat or raw visceral organs, liver and intestine, eating history from the rural inhabitants was found almost every case, and the eating place was in home, in the restaurant and in the tavern in order. 3. Incidence of human intestinal helminths was 78.6% from the 435 stool specimens examined, and the rate in Dae duck area was found higher rate than that of the Seosan, and the Taenia infection rate 6.5% in Daeduck residents and 0.9% in Seosan. 4. A total of 111 of Taeniasis cases were treated with Niclosamide, the worms, scolices and, or proglottids were expelled from 81 of the treated cases. The species identification with the 87 removed worms was performed, among of them 70 was T. saginata and 17 was T. solium. the ratio of both species was 4.7 : 1. 5. Examination of human cysticercosis from the out patient clinic of Chungnam National University Hospital as well as the local several clinics in Daejeon was observed in 17 cases. Subcutaneaus cysticercosis occurred all of the cases, and epileptic symptomes caused presumably by cerebral cysticercosis were also observed in 14 cases among of them, and Ocular cysticercosis occurred in the 3 cases out of 17 cases. 6. The incidences of Cysticercosis in the bovine was 5(0.6%) out of 803 slaughtered animals and 9(0.6%) out of 1522 slaughtered swine from the Daejeon and Dae duck public abattoir.

고양이, 돼지 및 오리의 망막에 존재하는 S-100 단백질의 분포에 대한 면역화학적 연구

김무강,김상근,이근좌 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.1 No.-

소의 뇌에서 처음으로 분리된 S-100 protein이 각 동물 망막에 존재하는 상태를 규명하기 위하여 S-100 protein antiserum을 이용하여 고양이,돼지, 오리의 망막을 면역화학적 방법으로 처리하여 그 분포상태를 조사하였던 바 다음과 같은 결론을 얻었다. 1. 고양이, 돼지, 오리의 망막에 분포하는 S-100 protein의 분포부위와 그 정도가 동물에 따라 서로 달랐다. 2. 고양이와 돼지에서는 각신경세포층 및 각 망상층에 모두 존재하였으나 오리에서는 각신경세포만 존재 하였다. 3. 각 동물 망막에 존재하는 S-100 protein의 분포를 규명하는 것은 앞으로 각 동물의 망막연구에 필요한 각 층들을 구별 하는데 좋은 지침이 될 수 있다.

절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.

鷄胎 羊膜囊의 輕視的 成長 變化에 關한 硏究

김무강,김상근 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

This paper dealt with the developmental changes of the amniotic surface area of the chick embryos from 5th to 9th days. Fetilized white leghorn 150 eggs were incubated and above 20 incubated eggs were freezed at random everyday in the refreezerater during 24 hr from the 5th incubated day to the 9th day serially and the chick amnions only containing chick embryos were photographied, and the plane surface area of the amnions were obtained by microcomputer image analyzer. These data were analyzed statistically and obtained results were as followings. 1. The amniotic surface area of chick embryos on each day of from the 5th to the 9th were 23.59±4.01 sq mm, 51.76±12.68 sq mm, 79.56±21.60 sq mm, 151.74±34.54 sq mm and 309.87±51.43 sq mm, respectively. 2. The rate of growth quantity of the amniotic surface area were increased by 2.19 on the day of the 6th to the 5th day, 3.37 on the day of 7, 6.43 on the day the 8th and 13.14 on the day 9th. 3. Coefficience variation on the day from the 5th to the 9th were 17%, 24.5%, 27.1%, 22.8% and 16.6%. 4. Except between 5th day and 6th day, the growth quantity between each day were increased significantly(P<0.001).

Mitomycin C가 鷄胎肝臟成長에 미치는 影響에 關한 硏究

金武剛,趙聲煥,金弘善,柳時潤,李康伊 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.1

Mitomycin C is well known as antibacterial, antiviral and antitumor by the mechanism of alkylation. The authors intend to analyze the effects of Mitomycin C on the weight of the liver in the chick embryos. On the sixth day of incubation, 8ug and 16㎍ of Mitomycin C were injected into the chorioalantoic sac of each incubated eggs of two groups. Between the twelth day and eighteenth day of incubation a daily total of thirty injected egges of each group was broken for weighting the liver weight of chick embryos. The effects of dose and difference of growth time were analyzed by Zimmerman's growth formula and Huxley's relative growth formula and determination of significance in the levels of difference was made by analysis of variance. The results are summarized as follows: 1. Mitomycin C suppresed growth of the liver in the chick embryos and growth inhibition appeared to be much stronger in the group of 16ug dosage than that of 8ug dosage. 2. Growth rate and specific growth rate were observed to be decreased in the liver with incubation day. 3. Growth center of the growth gradient for injected groups to the control groups in the liver were on the twelfth day. 4. Relative growth of injected groups to the control group were positive allometry in both injected group, and 16㎍ injected group was more remarkable allometry than 8ug injected group.

成長에 따른 肝細胞의 量的 變化에 關한 硏究

金武剛,金源式 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

To evaluate the morphological and functional changes of the hepatic cells of hybrid chick according to its growing stages, the volume of cytoplasm, nucleus and nucleolus were measured in 10 specimens of each group; the group of 2nd day, 14th day, 2nd month, 5th month and the group of 11th month after hatching. The results were as follows; 1. The volume of nucleus and nucleolus was increased from the 2nd day to the 14th day after hatching (actively growing period) and from the 5th month to the 11th month after hatching (the period of hypertrophy). The volume chage of cytoplasm appears oppositely to that of nucleus and nucleolus. 2. The nucleoplasmic index was increased at actively growing period and the period of hypertrophy, and the ratio of nucleolar volume to nuclear volume was increased at actively growing period, but unchanged at the period of hypertrophy.

鷄胎器官性長에 미치는 Mitoycin C의 影響에 關한 硏究

金武剛,金弘善,崔大卿,趙聲煥,金源式 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

Administration of Mitomycin C into various species of animals has widely been reported since last decade, however, none of them were utilized a growth formula proposed by Zimmerman and Huxley. In this paper we attempt to analyze the specific growth rate in general and organ relative growth rate using the chick embryo under the influence of Mitomycin C which is well known as an antibacterial, antiviral and antitumor by the mechanism of the alkylation. Congenital malformation of chick embryo were not considered. On the sixth day incubation, 8㎍ and 16㎍ of Mitomycin C were injected into the chorioallantoic and the egg was broken for weighing the body weight and individual organs such as brain, heart and lung on the day of 12th, 14th, 16th and 18th. The effects of the growth were analyzed by the analysis of variance method. The parameters included in growth quantity were obtained by the parameters included in growth quantity were obtained by the mathod of the last square using the measured weight, the formulas applied to analysis growth quantity and relative growth were : y=a+bt+ct^2, y=bx^2, and the results this obtained were summarized as follows : When injected with Mitomycin C, the chick embryos were observed with remarkable growth inhibition in all groups of the body, brain, lung and 14th groups of heart (p<0.005) and 12th, 16th, 18th groups of heart(p<0.05). Growth rate was increased with the development in the 8㎍ injected groups of the body, lung and 16㎍ injected groups of the lung, on the other hand, it was decreased in the both injected group of the brain and heart. The specific growth rate of all groups showed tendency of decrease in 8㎍ and 16㎍ Injected groups. The coefficient of relative growth of injected groups to control groups in the various organs showed positive allometry. The growth center of the growth gradient of all injected groups to control groups were on the eighteenth day.

Ⅱ. 食品 添加 許容色素(Amaranth, Brillant blue 및 Tartrazine)가 鷄胎 組織에 미치는 影響

金武剛,朴元學,李永春 忠南大學校 1972 論文集 Vol.11 No.-

This study was designed to observe microscopic changes of the several tissues in chick embryos given injection to the colours (Amaranth, Brilliant blue and Tartrazine). On the 6th days of incubation, 20㎎ and 2㎎ of the colours were injected into the chorioallantoic membrane. On the 18th days of incubation, chick embryos were opened and the several organs (liver, kidney, lung and brain) were made preparation of tissue. In the group given injection of 20㎎ amaranth and brilliant blue, microscopic changes were observed in the liver, kidney and lung. No evidence of microscopic changes due to treatment of 20㎎ and 2㎎ tartrazine were found. At level of 2㎎ amaranth and brilliant blue, no significant microscopic changes were seen. In the brain these colours had no sensitization activity.