Application of Artificial Insemination Technology for Dairy Breeding in Mongolia

Hyun‐Tae Jo,Jong‐In Jin,Seong‐Su Kim,Byung‐Hyun Choi,Tumor Baldan,Jung‐Gyu Lee,Yun‐Shik Kim,Sam‐Churl Kim,Il‐Keun Kong 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

This study was focused on improvement of milk production in Mongolia dairy industry by artificial insemination (AI) technology, of which was supported from ODA project of KOICA in Republic of Korea. The study was started from January 2009 to present and 3rd years in this year. So, all data, especially synchronization and pregnancy of dairy cows (Holstein) will be summarized in final result in this year. For synchronization, total 81 dairy cows selected from 4 private farms that were 38, 30, 8 and 5 in Undarmal milk, Onjin (Enkhbayer), Jargalant, and BRM School, respectively. All the dairy cows were injected intramuscular (IM) of 5 ml PGF2α in the vulva and detected estrus 2 to 3 days after PGF2α injection. Total 78 out of 81 dairy cows (96.3%) were detected estrus by only 1 time injection of PGF2α. The dairy cows that were induced estrus, inseminated with 0.5 ml dairy frozen semen by conventional AI techniques. The pregnancy diagnosis of the AI dairy cows was detected by uterus palpation after 60 days of insemination. Total 75 from 78 inseminated dairy cows (90.1%) were diagnosis pregnant. The estrus induction and pregnant rate were very effective using PGF2α injection and conventional AI techniques in Mongolia dairy cow. The results indicated that AI after estrus induction in Mongolia dairy cows could be applied to dairy breeding technology to improve the breeding efficiency and milk production.

한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

백서 간세포에서 세포외부 칼슘이온이 아드레날린성 약물에 의한 glycogen phosphorylase 활성도에 미치는 영향

최병주,성재현,김중영 慶北大學校 醫科大學 1991 慶北齒大論文集 Vol.8 No.1

The influence of extracellular calcium ions on adrenergic activation of glycogen phosphorylase was studied in isolated rat hepatocyte. Treatment of isolated hepatocytes with epinephrine, phenylephrine and isoproterenol elicited a dose-dependent increase in stimulation of phosphorylase activity. The time course of the activation of phosphorylase by adrenergic agonists was investigated in hapatocytes incubated either with a physiological concentration of extracellular Ca^2+ (2.6mM) or in a medium containing no added Ca^2+. The basal activity of phosphorylase was decreased by approximately 75% in hepatocytes incubated in the low Ca^2+ medium. The addition of adrenergic agonist caused a rapid increase of phosphorylase in cells incubated in the high or low Ca^2+ medium and enzyme activity decreased gradually after reaching a maximum 5 min after adrenergic agonists addition, and basal activity was not attained over the 10-min period of the time course. The magnitude of the maximal stimulatory effect was significantly decreased by the absence of extracellular Ca^2+. The stimulation of phosphorylase induced by epinephrine in the high or low Ca^2+ medium was no longer apparent after 30 min, but a second addition of epinephrine at this time can restimulate phosphorylase to an extent similar to that of a first addition. The addition of CaCl_2 to cells exposed to epinephrine and phenylephrine for 10 min in a medium having a low extracellular Ca^2+ produced a further increase of phosphorylase activity which was larger than the changes observed upon CaCl_2 addition to untreated cells. In order to obtain more information about the mechanism of Ca^2+ entry across the plasma membrane, we investigated the effects of calcium channel blockers on epienphrine-induced phosphorylase activation. Pretreatment of the diltiazem, verapamil, and nifedipine interfered with the magnitude of the maximal effect mediated by epinephrine; the kinetics of the enzyme activity in nifedipine-treated cells was found to be different from that of other channel blockers. These results suggest that entry of extracellular Ca^2+ into the cell is required in order to obtain a sustained adrenergic stimulation of phosphorylase activity and to supply the intracellular Ca^2+ store. The results also indicate that calcium channel blockers inhibit the transport of Ca^2+ across the plasma membrane through a putative Ca^2+ transporter which differs in nature from potential-operated calcium channels in excitable cells. J. Kyungpook. Nat. Univ. Sch. Dent. Vol.8, No.1, 105∼125, 1991.

정수처리 공정에서 철과 망간 잔류 농도에 관한 연구

최미리,서규태,문병현,이택순 7개 국립대학교 환경연구 논문집 공동발행 위원회 2003 공업기술연구 Vol.3 No.-

This study was to investigate the cause of the color and turbidity in a small-scale water treatment plant using lakes water as water supply source. Water qualities in aqueducts, in depth of the lakes and in each process were measured. And effects of prechlorination on each unit operation in the water treatment, especially on iron and manganese removal, were studied. The concentration of iron and manganese was 0.13, 0.08 mg/L in the surface water and 1.93 and 0.433 mg/L in 15 m depth of the lake. Higher iron concentration of the inlet was measured to 5.35 mg/L which was caused by the steel aqueduct corrosion. The optimun coagulation dosages of Poly Aluminum Chloride(PAC) and Liquid Aluminum Sulfate (LAS) were 17.5 mg/L and 35 mg/L. Pretreatmnet of raw water with CaCOl improved the removal efficiencies of iron and manganes. However, more efficient method for removing iron and manganes was required.

Seeds의 Crystallinity가 Cordierite의 상변태에 미치는 영향

최태현,전병세,이성우 慶南大學校 附設 工業技術硏究所 1994 硏究論文集 Vol.12 No.-

고밀도이며 고순도인 cordierite를 제조하기 위하여 solution sol-gel법과 α-cordierite의 seeding을 이용하였다. 시료를 분말과 monolith로 나누어 실험을 행하여 seed의 품질이 cordierite의 μ-type에서 α-type로의 상변태와 밀도에 관해서 각각 어떠한 영향을 미치는가에 대해서 알아보았다. 연구결과, 1000℃에서 합성한 seed용 분말보다 1300℃에서 합성한 seed용 분말이 더 α-cordierite 결정에 접근되어 있었고, 1300℃에서 합성한 seed를 첨가한 분말이 여러 범위의 온도에서 α-cordierite결정으로 가장 잘 발달하였으며, monolith의 경우 역시 1300℃에서 합성한 seed를 첨가한 것이 높은 밀도를 가지는 것으로 나타났다. 그러므로 cordierite의 homogeneity와 밀도는 첨가하는 seed의 crystallinity에 크게 좌우된다고 하겠다. Solution sol-gel(SSG) method and seeding were used to fabricate homogenious α-cordierite with high purity. The cordierite powders prepared by SSG and heat-treating at various temperature ranging 1000-1300℃ were added in the mixed or reacted with solution for seeding. Seed powder synthesized at 1300℃ was well developed to α-cordierite rather than any other seed powders at below 1300℃ and the quantity of α-phase was highest in the powder heat-treated at 1300℃ which had been added by seeding at 1300℃. The densities of all the monoliths were high because of existense of other phases like spinel. Therefore, the homogenity of resultant oxides depends on the crystallinity of seed.

안구의 비색소성 모양체 상피에서 발생한 유두상 선암종 -1예 보고-

최현주,최영진,이연수,이은정,강석진,김병기,심상인 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.12

치아 순면 형태가 측색 기기를 이용한 치아 색상 측정 결과에 미치는 영향

최용진,박수정,조현구,황윤찬,오원만,박병주,황인남 대한치과보존학회 2007 Restorative Dentistry & Endodontics Vol.32 No.5

열적 변화를 이용한 실내환경 가스의 분류

최낙진,심창현,송갑득,주병수,이윤수,이상문,이덕동,허증수 한국센서학회 2002 센서학회지 Vol.11 No.5

단일 기판위에 온도측정용 Pt 박막과 Pt 히터 그리고 가스 감지를 위하여 순수한 SnO_2 박막과 Pt가 첨가된 SnO_2박막을 설계하고 제작하였다. 제작된 소자는 실내환경가스인 이산화탄소, 프로판 그리고 부탄의 분류에 응용되었다. 동작 온도를 가변하기 위하여 히터의 입력 전압을 사다리꼴로 인가하면서 SnO_2 감지막의 반응특성과 실내환경가스들에 대한 분류여부를 조사하였다. 감지막 반응특성곡선에서 여러 변수들을 추출한 후 주성분분석(principal component analysis : PCA)을 통하여 가스 분류 특성을 검증하였다. Two SnO_2 based sensing films(pure SnO_2 and SnO_2/Pt) and a Pt thin film for temperature sensor on an alumina substrate were designed and fabricated for classifying the indoor environmental gases. By controlling the heating power in the shape of trapezoid, unique four sensing response curves created from both SnO_2 film and SnO_2/Pt film. Then, various parameters were extracted from sensing response curves and carried out principal component analysis(PCA). The results confirm that a sensor array with the proposed operating mode was extremely effective in classifying indoor environmental gases such as CO_2, C_3H_8, C_4H_10.

Methotrexate 치료를 받고 있는 활동성 류마티스 관절염 환자에서 etanercept의 유효성과 안전성에 관한 연구

최병렬,강태영,정청일,이혜순,엄완식,김태환,전재범,유대현,배상철 대한내과학회 2004 대한내과학회지 Vol.66 No.5

목적 : 류마티스 관절염을 앓고 있는 한국인 중에 기존 DMARDs로 치료 실패하였고, 고정용량의 MTX를 복용하고 있는 환자들에 있어서 etanercept의 유효성과 안전성을 평가하고자 하였다. 방법 : 기존 DMARDs로 치료 실패한 활동성 류마티스 관절염 환자 76명을 대상으로 하여 단일군, 공개시험을 하였다. 대상 환자들은 고정용량의 MTX를 복용하면서 etanercept 25 mg을 1주일에 두 번 피하 주사하였으며 12주간 투여하였다. 유효성은 ACR 20, ACR 50,조조강직 시간으로 평가하였고, 약제의 안전성은 이상반응 등으로 평가하였다. 결과 : 대상 환자는 총 76명으로 평균 연령은 45.2세, 남자 5명, 여자 71명이었다. 84.4%인 54명이 12주째에 ACR 20을 만족하였고, 53.1%인 34명이 12주째에 ACR 50을 만족하였다. 조조 강직 시간은 치료 전 203.3분에서 치료 12주째 42.6분을 평균 74.5% 호전되었다. 가장 흔한 이상반응은 주사부위 반응이었다. 이외에도 상기도 감염, 오심, 안면부종 등이 발생하였으나 심각한 부작용은 없었다. 결론 : etanercept는 효과적이고, 안전한 류마티스 관절염 치료 방법이며 특히 MTX치료에도 불구하고 활동성인 류마티스 관절염에 기대되는 치료라고 할 수 있다. Background : This study was performed to investigate the efficacy and safety of etanercept in active rheumatoid arthritis patients with stable dose of methotrexate in Korean. Methods : In a 12 week, single arm, open trial, we assigned 76 patients with active rheumatoid arthritis who had an inadequate response to disease-modifying antirheumatic drugs. Patients received twice-weekly subcutaneous injections of etanercept 25 ㎎ while containing to receive methotrexate at a stable dose of 7.5~25 ㎎ per week. The clinical response was defined as the percent improvement in disease activity according to the criteria of the American Collage of Rheumatology (ACR) at 12 weeks. Results : Etanrecept led to significant improvements in disease activity and was safe and well tolerated. At 12 week, 84.4% of the patients receiving 25 ㎎ of etanercept achieved a 20% ACR response, and 53.1% of those receiving etanercept achieved a 50% ACR response. The most common adverse event was injection-site reaction. Other advanse events were upper respiratory infection, nausea, and facial edema, but there were no serious adverse events associated with etanercept. Conclusion : In active rheumatoid arthritis patients, etanercept was safe, well tolerated, and provided rapid clinical improvements.

ℓ-Carnitine의 金屬錯化合物에 관한 硏究

崔秉箕,金順美,趙顯英 同德女子大學校 1987 同大論叢 Vol.17 No.1

In order to clarify transportation, the mode of action of intracellular ℓ-Carnitine related to the β-oxidation of fatty acid. metal (Cu++, Cd++, Mn++, Mg++, Ni++ And Zn++) coordination complexes of ℓ-Carnitine were studied on acid dissociation, stability constant, thermal stability, and functional radical chelated with metal by means of I.R. spectroscopy, differential thermal analysis and Bjerrum's method. The obtained results are summerized as follows: 1. The acid dissociation constant of ℓ-Carnitine in aqueous solution(μ=0.02) was found to be 6.3×10-5 at 25℃. 2. L-carnitine has formed metal coordination complex with Ni++, Co++, Zn++, Mn++, Mg++ an Cd+. From data calculated p[A-] and n, stability constants (log K₂ and log K₃) are obtainde; Metal Complex Cd Co Mn Mg Ni Zn log K₂ 2.7 - - 3.22 - - log K₃ 3.05 2.84 3.33 3.07 2.85 3.83 3. According to I.R. spectral data, amino and carboxyl groups of ℓ-carnitine are assumed to participate in the complex formation. 4. According to differential thermal analysis, each metal-carnitine coordination complex has been shown Mn>Zn>Cd>Mg>Ni>Co in thermal stability. 5. It is assumed that metal/ligand ratios has 1:3 approximately.