항코린에스테라제 독성에 대한 Histamine길항제의 효과

박은희,양근해,김오션 숙명여자대학교 약학연구소 1997 약학논문집-숙명여자대학교 Vol.13 No.-

This study was designed to examine the effects of antihistamines on the toxicity of neostigmine, a reversible cholinesterase inhibitor, in mice. Diphenhydramine and promethazine pretreatment increased the LD_(50) value of neostigmine by 2.15- and 3.65-fold, respectively. They also increased the onset times of toxic manifestations such as whole body tremor, loss of righting reflex and death. However, chlorcyclizine, chlorpromazine, i antazoline, chlorpheniramine and cimetidine pretreatment did not reduce the toxicities of neo-stigmine.

5-Fluorouracil과 그 유도체를 함유하는 Solid Lipid Nanoparticles 제조와 평가

서혜선,최명신,한규원,박소민,김길수 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.16

Solid lipid nanoparticles(SLNs) are particulate systems for parenteral drug administration and have good biocompatibility and stability. SLNs were prepared with lauric acid, as the lipid core. Tween 20 and tween 80 were used as surfactant. 5-fluorouracil and 1-benzoyl-5-fluorouracil were used as model drugs. Drug-loaded SLNs were prepared by the hot homogenization technique in order to evaluate the physical stability, entrapment efficiency of drugs as well as release profile. The particle size of SLNs was 40~600 nm. By increasing speed, the mean particle size of SLfs was decreased. And entrapment efficiency in the case of using 1-Benzoyl-5-fluorouracil was higher than using 5-Fluorouracil. The higher surfactant concentration, the faster release rate at the range of 1.5~2.5%.

강원도 원주지역 초·중·고등학생의 식습관 및 섭식행동에 관한 단면연구

허혜경,박소미,김기연,김춘배,안정숙,송희영,김기경 韓國保健敎育.健康增進學會 2003 보건교육건강증진학회지 Vol.20 No.1

Purpose : The purpose of this study was to investigate the dietary habits of students in order to identify risk groups according to their Body Mass Index (BMI), and to compare the eating behavior of students in the normal range (19<I≤24) and those in risk groups (BMI≤19, 24<I≤27, BMI>). Method : 1176 elementary school students, 850 middle school students and 672 high school City, were the participants The instrument for this study was a structured questionnaire that included demographic data as well as dietary habits, and the eating behavior instrument developed by Stunkard &essick(1985) and revised by Kim &im (1997). Result : 1) As students moved up in grade level their dietary habits became more irregular and the degree that students chewed food was reduced 2) As students moved up in grade level, a greater number of the students did not eat breakfast. The reasons given were that there were not enough time before classes started(40.4%) and a lack of appetite in the morning(10.6%) in high school students. 3) As for supper, from 67.8 to 81.9% of subjects reported having regular supper. However the rest of the subjects did not eat supper because of anorexia and fear of weight gain. 4) The results identified risk groups according to their BMI showed that for elementary school students, 55.9% were in the low weight group, 5.5% in the overweight group, and 0.9% in the obese group. For middle and high school students, 40.6% and 35.5% respectively were in the low weight group, 7.4% and 6.3% in the overweight group, and 4.1% and 2.5% the obese group. 5) Comparisons of the eating behavior of students in the normal weight group with that of those in the three risk groups showed that there were significant differences in 'hunger' and 'cognitive restraint of eating' in elementary school students, and significant differences in 'cognitive restraint of eating' in middle and high school students. Conclusion : The results of this study show that management of diet in school health sholud be addressed from both the aspect of lack of nutrition and that of excess nutrition. In other words, good diet is as important for students in the low weight group as it is f3r those in the overweight and obese groups. The estatblishment of good dietary habits and eating behavior in students, by nurses and dieticians should be done by providing repeated diet education and involvement in diet counseling.

In vitro 및 In vivo Assay를 통한 중금속의 에스트로겐성 평가

박철(Chul Park),김소정(So-Jung Kim),신완철(Wan-Chul Shin),김혜경(Hae-Gyoung Kim),최석영(Suck-Young Choe) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.9

식품오염 관련 중금속들의 에스트로겐성을 in vitro 와 in vivo 분석방법을 병행하여 평가하였다. 분석방법은 1) estrogen receptor dependent transcriptional expression 분석법, 2) E-screen assay 그리고, 3) 마우스 자궁비대시험 (uterotropic assay)을 사용하였다. 시험에 사용한 물질로는 17β-estradiol, diethylstilbestrol(DES), arsenic oxide, bis (tri-n-butyltin), cadmium chloride, chromium chloride, lead acetate, mercuric chloride을 사용하였다. Estrogen receptor dependent transcriptional expression 분석 결과, bis(tri-nbutyltin) > cadmium chloride > chromium chloride 순으로 에스트로겐성이 크게 나타났으며, mercuric chloride, lead acetate, arsenic oxide는 거의 나타나지 않았다. E-screen test 결과, bis(tri-n-butyltin) > cadmium chloride > chromium chloride 순으로 에스트로겐성이 크게 나타났으며, mercuric chloride, lead acetate, arsenic oxide는 거의 나타나지 않았다. 자궁비대시험 결과도 마찬가지로 bis(tri-nbutyltin), cadmium chloride, chromium chloride은 자궁중량 비대를 크게 초래하였으며, 반면에 mercuric chloride, lead acetate, arsenic oxide는 그러한 효과가 미약하거나 없었다. 세 분석방법 결과 bis(tri-n-butyltin), cadmium chloride, chromium chloride 순으로 에스트로겐성이 크게 나타났다. 이러한 결과는 최근 bis(tri-n-butyltin)과 cadmium chloride이 에스트로겐성이 있다는 다른 연구결과들과 잘 일치하며, 또한 크롬화합물도 에스트로겐성이 있다는 것을 새롭게 제시하고 있다. 본 연구는 세 단계 수준(전사활성화단계, 세포증식작용, in vivo assay)의 분석을 병행함으로써 수많은 중금속의 에스트로겐성을 효과적으로 평가할 수 있다는 것을 제시해주고 있다. The estrogenicities of six heavy metal compounds, which contaminate frequently in foods, were assayed using a combination of in vitro and in vivo assays. The assays were 1) estrogen receptor dependent transcriptional expression assay, 2) E-screen assay and, 3) the uterotropic assay in mice. The chemicals studied were 17β-estradiol, diethylstilbestrol (DES), arsenic oxide, bis(tri-n-butyltin), cadmium chloride, chromium chloride, lead acetate, and mercuric chloride. Using the estrogen receptor dependent transcriptional expression assay, the following estrogenicity ranking was measured: bis(tri-n-butyltin) > cadmium chloride > chromium chloride >> mercuric chloride >lead acetate = arsenic oxide. Using E-screen test, the following estrogenicity ranking was measured: bis(tri-n-butyltin) > cadmium chloride > chromium chloride >> mercuric chloride > lead acetate = arsenic oxide. Results from the uterotropic assay showed that bis(tri-n-butyltin), cadmium chloride, chromium chloride caused an increase in uterine wet weight, while lead acetate, mercuric chloride, and arsenic oxide failed to do so. Bis(tri-n-butyltin), cadmium chloride and chromium chloride showed the highest estrogenicity in three assay systems. Recent studies suggesting that bis(tri-n-butyltin), cadmium chloride have estrogenicities are compatible with the present finding. Furthermore, our study is suggesting that chromium chloride may be estrogenic. The results demonstrate that this three level-assay combination (transcriptional activation, cell proliferation, and an in vivo effect in an estrogen-responsive tissue) could serve as a useful method to assess the estrogenicity of heavy metals.

간호실무에서 신봉이론(Espoused theories)에 대한 탐색적 연구

서문자,김혜숙,이은희,박영숙,조경숙,강현숙,임난영,김주현,이소우,조복희,이명하,지성애,하양숙,손영희,권성복,김희진,추진아 성인간호학회 2001 성인간호학회지 Vol.13 No.1

As a nursing practice involves nurses' actions in a specific context of health care, this study has focused on exploring the espoused theories in nursing practice within the action science perspectives, Espoused theories are the belief, principles, and rationale expressed by the practitioner as guiding her/his actions in a situation of practice. The data were analysed qualitatively and 25 elements of espoused theories of nursing action were identified and clustered into 6 categories. The 25 elements of espoused theories are as follows: The clinical nurse worked in wholistic and individual nursing, focussed on the patient's needed, comfort and supportive nursing (5 theories of nursing goal) ; excellent skills, knowledge based, assessment and data collection, explaining, educating or a scientific basis(6 theories of nursing intervention): advocacy, value oriented, treatment, account- ability and commitment(4 theories of nursing ethics) ; human respect. partnership, trust(3 theories of patient-nurse relationship) : knowledgable, accumulated clinical experiences and personally lived experiences. positive perspectives(4 theories of nurse), role of intervention. rewarding peer relationship(3 theories of situations) The above mentioned espoused theories are similar to that of nursing textbooks which students learned through basic nursing education and almost the same as the Acts ofa Nurse in Korean. However, we are doubtful whether nurses actually do as they think. Therefore. it is recommended to review the theories-in-use in order to and any discrepancies between the espoused theories and the reality of nursing actions

여호와의 증인 환자에서 산과적 출혈 후 무수혈 치료 2예

김재령,여소진,이해혁,김정식,김태희,남계현,이권해,이임순,박진화,황경호 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Jehovah's Witness comprise a unique obstetric population. Their refusal of blood stems from an interpretation of a literal translation of the Bible, and it is this belief that puts them at an increased risk of morbidity and mortality if hemorrhage occurs. We report two cases of a Jehovah's Witness who bled massively due to obstetric hemorrhage, refused blood transfusion and had profound anemia. The patients were treated with recombinant human erythropoietin, parenteral iron and oxygen. And they were treated on an intensive care unit with intermittent positive pressure ventilation. We reviewed with literature considering the therapy for acutely anemic patients who refuse transfusion to decrease the duration of the most severe anemia.

선천성 판상 피부 골종 1예

이미애,김진형,황규광,박혜진,조소영 대한피부과학회 1999 大韓皮膚科學會誌 Vol.37 No.7

Osteoma cutis is a rare, benign, frequently congenital tumor of the skin composed of bone tissue, showing neither invasive nor metastasizing tendency. The term osteoma cutis is applied to cases of primary cutaneous ossification in which there is no evidence of Albrights hereditary osteodystrophy. Congenital platelike osteoma cutis is a clinically different variant of primary cutaneous osteoma. We report the case of a 10-month-old male infant who presented with two walnut-sized firm, poorly demarcated plaques containing pinhead-sized erythematous papules on the abdomen and the right leg and multiple variably sized erythematous subcutaneous nodules on the left leg sinee birth. The initial lesions did not enlarge nor did new lesions develop. A biopsy specimen of the abdominal lesion showed bony plate and cartilaginous structures in the subcutaneous fat tissue. There was no history of any skin disease prior to the development of osteoma.

[P5-58] Removal of Pesticides during Preparation and Fermentation of Baechu Cabbage Kimchi

So-Yeon Park,Ji-Kang Jung,So-Hee Kim,Ji-Young Yang,Soon-Ah Kang,Hae-Kyoung Chun,Kun-Young Park 한국식품영양과학회 2008 한국식품영양과학회 학술대회발표집 Vol.2008 No.10

Phophorylation of PKR aggravates neutrophilic asthmatic manifestations through bronchial epithelial cell activation with exaggerated ER stress

( So Ri Kim ),( Hae Jin Park ),( Yong Chul Lee ),( Kyeong Hwa Park ),( Yeong Hun Choe ),( Seung Yong Park ),( Jae Seok Jeong ) 대한결핵 및 호흡기학회 2018 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.126 No.-

Most asthma exacerbations are triggered by viral infections. The double-stranded RNA (dsRNA)-activated serine/threonine kinase R (PKR) is well characterized as an essential component of the innate antiviral response. However, to date, the role of PKR phosphorylation in bronchial epithelial cells is controversial. In this study, we investigated whether PKR phosphorylation in epithelial cells is involved in acute exacerbation induced by poly (I:C) and we aimed to define the interaction between PKR pathway and ER stress in bronchial epithelium leading to epithelial cell activation. We found that PKR inhibition using C-16 decreased severe asthmatic features and the expression of Th2 cytokines, IL-17, KC, and IFN-rin lung tissues. Interestingly, the expression of PKR, ER stress markers, and epithelial-derived cytokines was increased in lung tissues from mice sensitized with ovalbumin (OVA) and lipopolysaccharide (LPS) and challenged with OVA (OVALPS-OVA mice). Moreover, the expression of PKR and ER stress markers in LPS-stimulated normal human bronchial epithelial (NHBE) cells. These findings were attenuated significantly by the treatment with C-16. Besides, the administration of poly (I:C) aggravated all measurements in OVALPS-OVA mice. The administration of C-16 suppressed these exacerbated asthmatic features significantly. This study indicates that PKR phosphorylation plays an important role in acute asthma exacerbation, highlighting the potential of PKR inhibitor as a potent controller of bronchial epithelial cell activation in severe asthma and its acute exacerbation.

Ozone-induced COPD mouse model reflects the detrimental effects of aging as well as ozone on the lung

( So Ri Kim ),( Yong Chul Lee ),( Kyeong Hwa Park ),( Hae Jin Park ),( Navin Ray ),( Yeong Hun Choe ),( Seung Yong Park ),( Jae Seok Jeong ) 대한결핵 및 호흡기학회 2018 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.126 No.-

To understand the pathogenesis of chronic obstructive pulmonary diseases (COPD) and to screen new drug targets, the valid animal model is required. A cigarette smoke (CS)-induced model remains the most popular one. However, it usually requires 3-12 months to induce the COPD animal model by CS which is such a time-consuming and labor-intensive process. In this study, we aimed to establish the protocol for the ozone-induced COPD murine model and to differentiate the aging effects from the real ozone-induced detrimental effects on the lung in the mice. Ozone-induced COPD murine model was established by the exposure to 3 ppm of ozone twice a week for 7 weeks. Time-course analysis showed that the number of BAL cells was increased in air-exposed mice and ozone-exposed mice at the same age (i.e.,14 weeks) compared to young air-exposed mice (i.e.,6-7 weeks). The expression of TGF-β, IL-17, and IL-1β is gradually increased as ozone-exposed time goes by up to 7 weeks (TGF-β, IL-17) and 6 weeks (IL-1β), respectively. In addition, ozone-exposed mice for 7 weeks revealed that decreased lung function (FEV0.1) and increased alveolar destruction in lung tissues compared to air-exposed mice. Interestingly, old air-exposed mice (14 weeks) showed more severe lung destruction than young aged air-exposed mice (6-7 weeks). These findings suggest that ozone-exposed mice can be used for preclinical experiments regarding COPD as a better choice than CS-induced models and that this mouse model reflects the detrimental effects of aging as well as ozone on the lung more physiologically.