Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

원행을묘정리의궤 중 조리면에서 본 죽상 . 미음상 및 현륭원에서의 상차림고

김상보,이성우,한복진 한국식생활문화학회 1989 韓國食生活文化學會誌 Vol.4 No.4

To analyze dialy meal of royal meal, studied Jook Soora Sang (Rice Gruel), Mieum Sang (Thin Gruel) and Bankwa Sang, Soora Sang in Hyun Neung Won were on record Wonheng Ulmyo Jungri Euigwae (1795). Historic book "Jungri Euigwae" described the king's visit to his father's royal tomb "Hyun Neung Won", during the domain of Cheung Jo, the 22nd king of Choson Dynasty. The results obtained from this study are as follows. Rice Gruel (Jook Soora Sang), taken before breakfast, was arranged the same as the Soora Sang. The table for thin gruel (Mieum) was arranged in three kinds of sets; one for thin gruel, the others for stewed meat (Goem) and sugared fruit (Jeong Kwa). The diet seems to have been quit scientific and restorative. Bankwa Sang and Soora Sang in Hyun Neung Won was arranged the same as the other Bankwa Sang and Soora Sang. Therefore setting the table of royal meal had a rule.

用寒遠寒과 用熱遠熱에 대한 考察 : 六元正紀大論에서

채영진,신창환,임현택,여성원,한성규,이재원,정헌영,금경수 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

Five elements motion and six kinds of natural factors influence not only human disease but also difference of medicine effect. Because the climate of summer is very hot, that is easy to increase the properties of hot medicines, a doctor must be careful to use it in summer. Also the climate of winter is very cold, that is easy to increase the properties of cold medicines, a doctor must be careful to use it in winter. But in the certain conditions to use diaphoretics or purgatives, in spite of that regulation a doctor can use them.

일학습병행에서 학습기업의 재참여 의사에 미치는 영향 요인 탐색

조한진(Jo, Han-Jin),이선화(Lee, Seon-Hwa),박종성(Park, Jong-Sung) 한국경영교육학회 2020 경영교육연구 Vol.35 No.2

[연구목적] 기존 일학습병행에 참여한 학습기업의 재참여 활성화 방안에 앞서 학습기업의 재참여에 미치는 영향 요인을 구명하여, 학습기업 재참여 활성화 방안에 정책적 기초자료를 제공하는 것이다. [연구방법] 독립변수는 총 11개로 범주형 4문항과 연속형 7문항으로 구성되어 있고, 종속변수는 범주형 1문항으로 구성되어 이분형 로지스틱 회귀분석을 적용하였다. [연구결과] 전체적으로 학습기업의 재참여 의사에 영향을 미치는 요인은 참여 동기, 일학습병행 전반적인 만족, 현장훈련 시간 단축 필요성, 기업현장교사 양성교육 기간 적절성, 일학습병행 훈련과정 종료 후 유지 계획 유무, 참여유형의 6가지 변수로 나타났다. 참여유형을 재직자단계와 재학생단계로 나누어 분석한 결과는 다음과 같다. 첫째, 재직자단계에서 학습기업의 재참여 의사에 영향을 미치는 요인은 참여 동기, 일학습병행 전반적인 만족, 현장훈련 시간 단축 필요성, 기업현장교사 양성교육 기간 적절성, 일학습병행 훈련과정 종료 후 유지 계획 유무의 5가지로 나타났다. 둘째, 재직자단계에서 학습기업의 재참여 의사에 영향을 미치는 요인은 참여 동기, 학습기업 선정 절차 적절성, 학습기업 선정기준 적절성, 일학습병행 전반적인 만족, 일학습병행 훈련과정 종료 후 유지계획 유무의 5가지로 나타났다. [연구의 시사점] 일학습병행의 내실화를 위하여 학습기업의 재참여 활성화 방안을 위한 정책적 시사점을 제공하였다. [Purpose] The purpose of this study is to examine the factors affecting re- participation of companies involved in apprenticeship before the revitalization of re-participation and to provide basic policy data to revitalize re-participation of companies involved in apprenticeship. [Methodology] There were 11 independent variables comprising four questions for categorical variables and seven questions for continuous variables. One dependent variable comprised one question for a categorical variable. The binomial logistic regression analysis was applied. [Findings] The factors affecting re-participation intention of companies involved in apprenticeship were six variables, including the motivation to participate, overall satisfaction about apprenticeship, need for the shortened time of on-the- job training, appropriate training period of in-company trainers, plan to maintain apprenticeship after the end of the training process, and participation type. The results of analyzing participation type First, there were five factors affecting re-participation intention of companies involved in the apprenticeship for students, including the motivation to participate, overall satisfaction about apprenticeship, need for the shortened time of on-the-job training, appropriate training period of in-company trainers, and plan to maintain apprenticeship after the end of the training process. Second, there were five factors affecting re-participation intention of companies involved in the apprenticeship for new workers, including the motivation to participate, appropriate procedure for selecting companies involved in apprenticeship, appropriate criteria for selecting companies involved in apprenticeship, overall satisfaction about apprenticeship, and plan to maintain apprenticeship after the end of the training process. [Implications] Policy implications were presented to substantialize apprenticeship and to revitalize re-participation of companies involved in apprenticeship.

進饌儀軌를 통하여 본 1887년 朝鮮王朝 宮中 進饌宴 중 萬慶殿 翌日灰爵과 萬慶殿 翌日夜연의 상차림에 대한 考察

金尙寶,李盛雨,朴惠苑,韓福眞,黃蕙性,韓福麗 동아시아식생활학회 1991 동아시아식생활학회지 Vol.1 No.3

To examine the following day party and the following evening party in the Man Kyong Jeon, the authors studied "Jin Chan Eui Gue", which is a historical reoord published in 1887(King Go-Jong). The results obtained from the study were as follows, 1.There were fixed arranging tables(22) and taking-away tables in the dining table of the following day party. 2.There were fixed arranging tables(5) and taking-away tables in the dining table of the following evening party. 3.Food and flowers were distributed to the 390 attendants in the following day party. 4.There were no distribution of food and flowers to the attendants in the following evening party. 5.Arrangements of the followings day party and following evening party were the same as those of the main party. 6.The arranged seat of the King, Go-Jung was facing the south. 7.According to the status or class of the attendant, the pattern or size of table settings were differentiated in the kinds of food, heights of food, table flowers, chinaware etc. 8.1n the arrangements of tables for the King, the heights of food on the dishes were as following levels, 1 dish : 1 chok(1尺) 6 dishes : 8 chon(8寸) 4 dishes : 7 chon(7寸)

다발성 말초신경병증의 조기진단 : 적외선 체열촬영검사와 전류지각역치검사의 조합진단 Combination Diagnosis Using by Digital Infrared Thermogram and Current Perception Threshold

이삼규,김재형,최인성,이소영,한재영,조성렬,김재휴,노성만,정민영,정동진,김현성 全南大醫大 2001 전남의대학술지 Vol.37 No.3

경도관 동맥색전술을 이용한 비정상 자궁출혈의 치료

양승부,최교창,이상진,정영진,임한혁,한효상,황인철,조환성 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Objective : Our purpose of this study is to evaluate the effectiveness of the transcatheter arterial embolization for the management of abnormal uterine bleeding. Materials and Methods : 14 patients with massive or recurrent uterine bleeding underwent percutaneous transcatheter arterial embolization between February 2003 and September 2004. We reviewed 14 cases of uterine artery or internal iliac artery embolization using gelfoam or PVA particles. Results : Good management of uterine bleeding was achieved in 13 of 14(93%) cases. The cause of abnormal uterine bleeding was myoma(5 case), post D & E bleeding(3), acquired vascular malformation(3), pseudoaneurysm(1), adenomyosis(1), and endometrial hyperplasia(1). Conclusion : Transcatheter artery embolization is an effective and life-saving procedure in massive or recurrent uterine bleeding. Early diagnosis and prompt transcatheter arterial embolization is a useful mangement of uterine bleeding.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

80년代를 向한 農村住居環境計劃에 關한 硏究

韓相謙,朱京在,金龍八,李好璡 건국대학교부설 산업기술연구소 1978 논문집 Vol.4 No.-

In the past, there were many differences between living standard of urban area and that of rural area. And annual gross income of rural inhabitants did not reach that of urban laborers, but from 1974, income of rural dwellers became to reach over urban laborers' income. in early of 1980s, annual gross income of rural dwellers will be increased to ￦1,400,000 per house, which will encourage them to improve their living environment. To improve existing rural living standard to livable and decent living environment, it would be required to reform rural community function, its size and new living zone out of consideration for increase of annual gross income, and change of living pattern, their convention, size of production, cultivating method and income sources in rural areas. Through the investigation of existing environment conditions in rural area, we selected Homyong-Myon, Yechon-kun, Kyongsang-Bukdo as case study and tried to make actual rural planning of this area to show sampling study of rural renewal planning. It is recommendable that community facilities and existing living zone in rural area should be rearranged for the reasonable harmony with improved living environment and matching with existing administrative district as possible, new living zone had better be classified into 4 categories, as below: 1)Basic Living Zone This would include 50-70 households within about 1km semidiameter boundary where daily activities of inhabitants would be conducted. 2)First Living Zone This would include about 5-10 basic living Zones within about 2-3km semidiameter boundary where weekly activities of inhabitants would be conducted and elementary school facilities would be main focus of this community area. 3)Second Living Zone This would be main living Zone in rural community area and include 3-4 first living Zones within 4-6km semidiameter boundary where secondary school and Myon district office would be located and monthly activities of inhabitants would be main community behaviors in this area. 4)Third Living Zone This would be matching with Gun district living community and synthetic living center in rural community area within 15-30km semidiameter boundary. This thesis just shows convenient division of living Zones for decent rural community environment and functional relationships between each living Zones, but further study should be proceeded for the precise planning of community facilities-their location and scope of size in each living zone.