Analysis of KAP1 expression patterns and human endogenous retrovirus Env proteins in ovarian cancer

Kyung‑Yoon Jeon,Eun‑Ji Ko,Young Lim Oh,Hongbae Kim,Wan Kyu Eo,김아리,Han Gyu Sun,Meesun Ock,Ki Hyung Kim,Hee‑Jae Cha 한국유전학회 2020 Genes & Genomics Vol.42 No.10

Background Human endogenous retroviruses (HERVs) constitute around 8% of the human genome and have important roles in human health and disease, including cancers. Previous studies showed that HERV envelope (Env) proteins are highly expressed in cancer tissues and co-related with cancer progression. KAP1 has been reported to play a key role in regulating retrotransposons, including HERV-K, through epigenetic silencing. Objective The relationship between KAP-1 and HERV Envs expressions was analyzed only in tumor cell lines and has not yet been studied in cancer tissues. In this study, we analyzed the expression patterns and relationship between KAP1 and HERV Env proteins in ovarian cancer tissues. Method The expression patterns of KAP-1 and HERV Env proteins, including HERV-K and HERV-R, were analyzed in ovarian cancer tissue microarrays that contained 80 surgical specimens, including normal ovary and malignant ovarian cancers. Results The expression of HERV-R Env and KAP1 proteins is signifcantly higher in ovarian cancer compared with normal ovary tissues. However, the expression of HERV-K Env did not change signifcantly in cancer tissues. The expression patterns of HERV-K Env and HERV-R Env signifcantly increased in early stages of cancer and KAP1 expression was higher in certain stage and types of cancers. However, the expression of HERV-K Env, HERV-R Env, and KAP1 did not change in diferent age groups. The correlation between the expression of KAP1 and HERV-Env, including HERV-K and HERV-R, was not signifcantly correlated. Conclusions The results of this study showed that there was no signifcant correlation between the expression of KAP1 and HERV Env proteins in ovarian cancer tissues, unlike studies with cell lines in vitro. These results suggest that the actual expression of HERV Env proteins in ovarian cancer tissues may be regulated through various complex factors as well as KAP1.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

강원도 화천군 수달(Lutra lutra) 서식지의 식생 구조

서형수 ( Hyung Soo Seo ),신영섭 ( Young Seob Shin ),이경은 ( Kyung Eun Lee ),김윤미 ( Yoon Mi Kim ),전미나 ( Mina Jeon ),남택우 ( Taek Woo Nam ),한성용 ( Sung Yong Han ),정연숙 ( Yeonsook Choung ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.special

In order to determine whether vegetation would be one of the factors for the selection of otter home range, vegetation structure and other potential factors were studied in Hwacheon, Korea. Thirteen sites, otter’s activity found and not found, were investigated in North Han River and connected tributary streams of Hwacheon-gun. Three types of vegetation were classified by cluster analysis, which is short grass, tall grass and shrub type. Vegetation zone of each channel is composed of either one type, or mosaic of tall grass and shrub type. Short grass type is common in Lake Paro and upper North Han-river where water level is highly variable throughout a year. Therefore, annual species such as Persicaria nodosa, Fimbristylis dichotomam and Chenopodium ficifolium are the most dominant. Shrub type is common at the downstream sites of Jichon stream and along mainstream of North Han River down Lake Paro. A shrub species, Salix koreensis, is the most common. Tall grass type is dominant occupying the most vegetation zone of the tributary channels. Phragmites japonica is absolutely dominant. Due to its dense cover, a few plant species are co-existed. Otter activity was found in all three vegetation types and no marked activity was found at some sites of tall grass type. There is no difference in species composition and physiognomy between tall grass sites with and without otter activity, while it shows significant difference in fish availability between two groups. Overall we found that home range of otters in the region is along the mainstream and downstream of tributary streams with high fish availability in all vegetation types and in various human activity levels.

우리나라 한중콘크리트 적용기간의 변화에 관한 고찰

한민철,김경민,신동안,윤기원,한천구 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this paper. variations of the period of cold weather concrete in Korea are discussed based on the climate data of Korean Meteorological Adminstration(KMA). Determination of the period of cold weather concrete with the region is done by following Korean Concrete Institute(KCD, American Concrete Institute(ACD and AIJ(Architectural Institute of Japan(AIJ) specification. Present study is compared with previous study done by the authors. Period of cold weather concrete is found to increase with high altitude and in-land and mountain area. Period of cold weather concrete by present study is shorted compared with that of previous study by about 3~6 days regardless of applied specification. Especially. the period of cold weather concrete at southern region near sea is reduced remarkably compared with that of previous study. This is due the rise of mean air temperature by global warming. In the scope of present study, period of cold weather concrete is provided with table and map diagram.

Bacillus thuringiensis var. israelensis의 포자와 crystal생성에 관하여

한만덕,윤경하 순천향대학교 1985 논문집 Vol.8 No.2

Bacillus thuringiensis var. israelensis(B.t.i)의 내생포자와 crystal의 생성을 높이기 위하여 몇 가지 배양조건들을 조사하였다. B.t.i를 1.5% soluble starch, 0.5% glucose, 1.0% yeast extract, 0.2% K₂HPO₄, 0.01% MgSO₄.7H₂O, 0.02% CaCl₂, 0.002% FeSO₄.7H₂O, 그리고 0.002% MnSO₄.4H₂O로 구성되는 배지(pH7.0)에서 30C로 진탕배양(200rpm)했을때 배양 32시간에 균주의 생장이 최대가 되었다. 배양 72시간에 수획한 포자와 crystal복합체의 농도가 0.05㎍/㎖일때 Aedes togoi유충은 12% 죽었고 0.01㎍/㎖농도일 때 Culex pipiens 유충은 15% 죽었다. 한편 포자와 crystal복합체의 농도가 0.5㎍/㎖일때 Aedes togoi와 Culex pipiens유충은 모두 죽었다. In order to increase the production of spore-crystal complex of Bacillus thuringiensis var. israelensis which is toxic to mosquito larvae, some culture conditions of the culture were investigated. 1. The effective culture medium for the production of spore by the culture contained 1.5% soluble starch, 0.5% glucose, 1.0% yeast extract, 0.2% K₂HPO₄, 0.01% MgSO₄.7H₂O, 0.02% CaCl₂, 0.002% FeSO₄.7H₂O and 0.002% MnSO₄.4H₂O. 2.The population density of the culture was at a maximum after 32 hours of incubation when it was grown in the effective medium at 30C with rotary shaking. 3.Spore-crystal complex of B. thuringiensis var. israelensis which was grown in the effective medium exhibited toxicity to Aedes togoi larvae at 0.05㎍/㎖ and Culex pipiens larvae at a concentration 0.01㎍/㎖ respectively. One hundred percent of Aedes togoi and Culex pipiens larvae were killed at a dosage rate of 0.5㎍/㎖

운동처방에 의한 써키트웨이트트레이닝이 심박수 및 근력에 미치는 효과

윤상화,이한경 龍仁大學校 1991 용인대학교 논문집 Vol.7 No.-

The students who had experience of the physical education 12 majors and 12 non-majors have been chosen at discretion and trained for 12 weeks so as to examine the effect of Circuit and Weight training by exercise Prescription on their heart rate and muscular strength. And the results are as follows. 1. Heart Rate in the stable Conditions reflected G-1(-2.06 beats /min) and it showed the significant of (p<0.001) at the point of G-2(-4.08 beats /min). 2. Left Grip Strength showed the significant increase of (p <0.001) rate at both G-1(+3.95kg), G-2(+3.5kg) and Right Grip Strength showed the significant increase of (p<0.001) rate at both G-1(+3.87kg), G-2(3.69kg), Back Muscule Strenght in case of (p<0.001) rate at both G-1(+l7.86kg), G-2(17.44kg).

요골동맥을 이용한 관동맥 중재시술의 경험

윤정한,이승환,김장영,이한요,이명옥,김승년,황성오,최경훈 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.9

위암환자에서 BrdU에 의하여 유도되는 Fragile sites의 발현

윤숙자,한승로,정옥,김수일,박경란,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

The expression frequency of BrdU-induced fragile sites was examined in stomach cancer patients to determine whether this parameter could be used as a marker of genetic susceptibility in at-risk individuals. G banding performed to detect fragile sites. No difference was found in expression frequency between the stomach cancer patients and a group of normal individuals(p=0.7). This indicates that the expression frequency of BrdU-induced fragile sites is not a suitable marker for assessing genetic susceptibility in stomach cancer patient.

영지버섯(Ganoderma lucidum)의 균사체 생산에 관하여

한만덕,이권행,윤경하 순천향대학교 1992 논문집 Vol.15 No.3

약효가 다양한 영지버섯의 균사체 생산에 최적인 배지를 조성코저 몇가지 실험을 통하여 아래와 같은 결과를 얻었다. 1.영지버섯을 2% soluble starch, 0.5% yeast extract, 0.5% KH₂PO₄, 0.01% MgSO₄.7H₂O그리고 0.001% K₂HPO₄로 조성한 배지에서 27℃로 7인간 진탕배양(120 rpm)했을때 16.38mg/ml로 최대의 균사체를 얻었다. 2.최적 배지내에서 균주의 배양 4일까지는 탄수화물을 서서히 감소하고, 환원당을 급격히 증가하였다. 그러나 배양4일 이후부터는 탄수화물과 환원당 모두 급격히 감소하였다. 3.최적 배지내에서 pH는 초기에 5.5에서 배양 3일째에 4.0으로 떨어져 배양 7일까지 지속되었다. In order to increase the mycelial production of Ganodermalucidum which exhibits various phamacological effects on mammalian, some ingredients of medium were investigated. 1.The effective culture medium for the mycelial production by the culture contained 2% soluble starch, 0.5% yeast extract, 0.5% KH₂PO₄, 0.01% MgSO₄.7H₂O and 0.001% K₂HPO₄ 2.The maximum yield of the mycelium of the culture was 16.38mg/ml when it was grown in the effective medium with rotary shaking (120 rpm) at 27℃ for 7 days. 3.During the culture of the strain in the effective medium, total carbohydrate in medium was decreased with the culture time but reducing sugar rapidly increased by the three days of culture time; it was decreased three days later. 4.The pH in the effective medium was decreased from 5.5 at the initial culture time to 4.0 on the third day but was maintained till the seventh day since then.

요골동맥을 이용한 관동맥 조영술의 유용성

윤정한,이승환,이한요,김장영,김일회,최윤종,이형준,이명옥,김승년,황성오,홍인수,최경훈 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.10