Role of Calcium in Function of Isolated Perfused Rabbit Kidney

이권행,전은의,홍경자,조규철,Lee, Kweon-Haeng,Chun, Eun-Eui,Hong, Kyoung-Ja,Cho, Kyu-Chul The Korean Society of Pharmacology 1986 대한약리학잡지 Vol.22 No.2

저자는 적출관류 토끼 신장기능에서 칼슘의 역할과 furosemide의 이뇨작용에 미치는 영향을 관찰하여 다음과 같은 결과를 얻었다. hydralazine놔 verapamil을 주로 신혈관 저항의 감소로 이뇨작용을 나타냈다. furosemide를 hydralazine 또는 verapamil 병합투여시 뚜렷한 이뇨작용이 나타났으며 두군 사이에 차이는 없었다. quinidine은 신혈관 수축을 일으켰으나 이뇨작용이 나타났으며 furosemide의 이뇨작용을 항진시켰다. 칼슘제외된 관류액으로 관류시 칼슘은 신혈관 수축에도 불구하고 이뇨작용이 나타났으며 furosemide의 이뇨작용을 항진시켰다. quinidine은 칼슘이 제외된 상태에서 신장기능 및 furosemide의 이뇨작용에 영향을 미 치지 못하였으나 calcium 병합투여시 과도한 신혈관 수축으로 항 이뇨작용을 보였다. verapamil은 칼슘이 제외된 상태에서 약간의 이뇨작용을 보였지만 칼슘 및 furosemide의 이뇨작용을 변화시키지 못하였다. 이상의 성적은 적출관류 토끼 신장에서 칼슘, verapamil 및quinidine이 이뇨작용이 있으며 칼슘은 furosemide의 이뇨작용을 항진시킨다. This study was designed to investigate the role of calcium in the function of an isolated perfused rabbit kidney and its effect on the diuretic action of furosemide. The administrations of hydralazine and verapamil produced remarkable diuretic actions mainly by decreasing renal resistance. The administration of furosemide in combination with hydralazine or verapamil produced remarkable diuretic action and there was no difference between the two groups. The administration of quinidine produced a diuretic action in spite of vasoconstriction and potentiated the diuretic action of furosemide. In the calcium-free perfusion medium, the administration of calcium produced a marked diuretic action in spite of vasoconstriction and potentiated significantly the diuretic action of furosemide. The administration of quinidine did not alter renal function and the diuretic action of furosemide, but the combined administration of quinidine and calcium showed antidiuretic effect due to excessive vasoconstriction in the calcium-free perfusion medium. Although the administration of verapamil produced a slight diuretic action in the calcium-free perfusion medium, verapamil did not alter the diuretic action of calcium as well as the diuretic actions of furosemide alone and in combination with calcium. The results of this experiment show that calcium, verapamil and quinidine produced diuretic actions and calcium potentiates the diuretic action of furosemide.

질환유전체 연구센터 ; 3 . 뇌신경계질환 유전체 연구센터

이권행 생화학분자생물학회 2001 생화학분자생물학회 소식 Vol.21 No.1

N/A N/A

적출관류 토끼 신장기능에 대한 키닌의 영향

이권행,성기욱,홍경자,이상복,조규철 대한신장학회 1988 대한신장학회잡지 Vol.7 No.1

Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상호관계

이권행,이준우,한만덕,정훈,김영일,오두환,Lee, Kweon-Haeng,Lee, June-Woo,Han, Man-Deuk,Jeong, Hoon,Kim, Young-Il,Oh, Doo-Whan 한국미생물·생명공학회 1994 한국미생물·생명공학회지 Vol.22 No.1

Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and researcher reported that antitumor activity of polyasccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein $L_{A}$, $L_{B}$ and $L_{C}$ within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein $L_{A}$, $L_{B}$ and $L_{C}$.

Ganoderma lucidum IY 009조다당 분획들의 항암활성과 항보체활성간의 상관관계

이권행,이준우,한만덕,정훈,김영일,오두환 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.1

항암활성 다당류들은 mouse의 보체계를 활성화시키고 serum내 특정 혈청 단백질을 증가시키는 것으로 알려져 왔다. 여러 연구자들의 의하면, 다당류의 항암활성은 보체계의 활성화와 mouse serum의 특정 단백질인 L_A, L_B 및 L_C등의 증가와 관련이 있다고 보고하였다. 6종의 Ganoderma lucidum의 균사체로부터 얻은 조다당의 항암활성과 항보체활성 사이에는 상관관계가 없었으나, G. lucidum IY 009의 균사체를 알칼리로 추출하여 얻은 조다당 분획- AS, T, AI 및 M 사이에서 sarcoma 180에 대한 항암활성이 높은 것이 항보체활성이 높게 나타났다. 한편 AS, AI 및 M을 투여한 mouse의 serum중의 L_A, L_B 및 L_C protein의 강도는 항암활성과 비례적으로 나타났으나, 열수추출 분획 H의 경우는 비례하지 않았다. Antitumor polysaccharides were known to activate complement system and to increase specific serum proteins in mouse, and some researcher reported that antitumor activity of polysaccharides might be correlated with their biological properties such as activation of complement system and increase of specific protein L_A, L_B and L_C within the mouse serum. In case of several Ganoderma lucidum, there was no correlation between their antitumor activities and their bioloical properties, but the antitumor activities against sarcoma 180 of the alkali extracted crude polysaccharide fractions of the Ganoderma lucidum IY 009. AS, T, AI and M were correlated with their bioloical properties such as anticomplementary activity and intensity of mouse serum protein L_A, L_B and L_C.

Ganoderma lucidum IY 009 로 부터 분리된 항암성 다당류의 정제 및 구조분석

이권행,정훈,이준우,한만덕,최경숙,유주현,오두환 한국미생물생명공학회 1992 한국미생물생명공학회 학술발표초록집 Vol.1992 No.-

Ganoderma lucidum IY 009로 부터 분리된 항암성 다당류의 정제 및 구조분석

이권행,정훈,이준우,한만덕,최경숙,오두환 한국산업미생물학회 1994 한국미생물·생명공학회지 Vol.22 No.2

Ganoderma lucidum IY 009로부터 얻어진 조다당 분획, AS분획 0.2 g을 물에 녹여 DEAE cellulose(OH form)로 충진된 column에서 물, 0.1M NaHCO_3, 0.3M NaHCO_3, 0.5M NaHC0_3 및 0.5 N NaOH등으로 차례로 용출시켜 AS-1분획, AS-2분획, AS-3분획, AS-4분획 및 AS-5분획을 각각 얻었다. AS-1, AS-2, AS-3, AS-4 및 AS-5의 sarcoma 180에 대한 암저지활성은 각각 97.5%, 68.0%, 73.0%, 81.0% 및 66.0%로 물로 용출된 중성다당 분획인 AS-1가 가장 우수하였다. AS-1분획은 Sepharose CL-4B column에서 용출시켰을 때 단일 peak를 보였으며, 분자량은 약 580,000 dalton이었다. AS-1분획의 당 함량은 98.9%로 mannose 67.5%, xylose 22.5%, glucose 5.8%, galactose 1.8% 및 ribose 2.4%로 구성되어 있으며, I.R. spectrum 864.3㎝^-1에서 흡수파장이 나타나 α-xylomannan이라고 생각되었다. AS-1의 주요 methylated alditol acetate는 1,4-Di-0-acetyl-2,3,5-Tri-0-methyl-xylitol, 1,4,5-Tri-0-acetyl-2,3,6-Tri-0-methylmannitol 및 1,3,4,5-Tetra-0-acetyl-2,6-Di-0-methylmannitol로 확인되었고, molar ratio는 대략 1 : 2 : 1였으므로, AS-1의 core portion은 (1→4)linked mannopyranosyl unit로 매 3개의(1→14)linked mannopyranosyl residue마다 1개의 xylopyranosyl residue가 mannopyranosyl residue와 (1→3)결합을 하고 있는 structural unit를 가진 xylomannan으로 추정되었으며, structural unit의 중합도는 약 835였다. Alkali soluble(AS) fraction, revealed the highest antitumor activity of the alkali extracted fractions of G. lucidum IY 009, was loaded on DEAE cellulose(OH form) column. AS-1, AS-2, AS-3, AS-4 and AS-5 were obtained by stepwise elution with H_2O, 0.1 M NaHC0_3, 0.3 M NaHCO_3, 0.5 M NaHC0_3 and 0.5 N NaOH respectively, and their antitumor activities(I.R. %) against the sarcoma 180 were 97.5%, 68.O% 73.0%, 81.O% and 66.O% respectively. AS-1 observed highest antitumor activity was appeared as single peak on the Sepharose CL-4B column chromatography, and their molecular weight was about 580,000 dalton. The carbohydrate content of AS-1 was 98.9%, their monosaccharide consisted of 67.5% of mannose, 22.5% of xylose, 5.8% of glucose, 1.8% of galactose and 2.O% of ribose. AS-1 was assumed α-linkaged xylomannan having infrared absorption at 864.3 ㎝^-1. The main alditol acetates of AS-1 were identified as 1,5-Di-O-acetyl-2,3,4-Tri-0-methylxylitol, 1,4,5-Tri-0-acetyl-2,3,6-Tri-0-methylmannitol and 1,3,4,5-Tetra-0-acetyl-2,6-Di-0-methylmannitol by methylation analysis, and their molar ratio was 1 : 2 : 1. The core portion of AS-1 might be α-(1→4)mannopyranosyl unit branched with side chain, C1 of xylopyranosyl residue linked to C3 of every 3 mannopyranosyl units, and the degree of polymerization of structural unit in AS-1 was about 835.

독성학에서의 Free Radical

이권행 가톨릭대학 산업의학 센타 산업의학연구소 1995 韓國의 産業醫學 Vol.34 No.2

중금속 등을 포함하는 여러 물질들에 의해 유도되어 일어난 화학 반응중 생긴 대사물 중 쌍을 이루지 못한 하나 이상의 전자를 갖는 독립적인 존재들 free radical이라고 한다. 생체에서 free radical의 발생화정과 생리적인 역할은 정확히 밝혀지지 않고 있으나 여러 질병의 병인에서 주요역할을 하는 것으로 알려지고 있다.free radical이 발병 과정에서 주요 역할을 하는 것으로 알려진 질병은 많이 존재하며 다음과 같이 소개하고자 한다.

토끼 신장조식에서 무기이온과 요소의 농도

이권행,조규철 최신의학사 1989 最新醫學 Vol.32 No.12

토끼 신장조직에서 아미노산농도의 변화

이권행,조규철 中央醫學社 1989 中央醫學 Vol.54 No.12

Several organic solutes -have been identified as osmotically active organic solutes or osmolytes in the medulla of mammalian kidney. Because high levels of inorganic salts and urea inhibit various cellullar enzymes, non-. perturbing organic solutes are more proper than perturbing solutes for adaptation to high salt environment. Among these osmolytes, polyols, methylamines and amino acids are found in the medulla of mammalian kidney. Even though there is no direct evidence, some authors suggest that amino acids play a role of osmolytes in the mammalian kidney. The kinds and metabolism of osmolytes are very complex. Also variables. such as sample preparation and detection method and experimental condition deviate the true levels of osmolytes in the kidney. In an attempt to clarify the role of amino acids as osmolytes in the rabbit kidney and to select the proper time of the sample preparation for amino acids, we measured the levels of amino acids in rabbit kidney tissues with time course. The concentrations of taurine, aspartic acid, glutamine, serine and alanine in the medulla were above 2 mM/kg wet weight and higher than in the cortex. Those levels in the medulla were decreased gradually with time course, but those in the cortex were increased gradually. Of amino acids in rabbit kidney, the concentrations of glycine, the highest one, in the medulla and cortex were 5.89 ± 2.44 mM/kg wet wt and 5.56 ± 1.12 mM/kg wet wt respectively. With time course, glycine level in the cortex was increased to 7.54 ± 3.95 mM/kg wet wt. p The concentrations of valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine and tryptophan in the kidney tissues were below 1 mM/kg wet wt. Lysine and arginine did not show special patterns. The results of this experiment show that amino acids in rabbit kidney play a role as osmotically active organic solutes and the sample has to be prepared immediately after the extraction of kidney for amino acid detection.