Development of a bivalent live vaccine against duck hepatitis A virus types 1 & 3 and vaccine program for breeder ducks in Korea

Jae?Hee Roh(노재희),Min Kang,Bai Wei,Se-Yeoun Cha,Jeong-Gon Cho,Hyung-Kwan Jang 한국가금학회 2017 한국가금학회 정기총회 및 학술발표회 Vol.2017 No.11

어린 오리에 치명적인 질병을 일으키는 오리바이러스성간염의 예방을 위해 혼합생백신 개발, 백신의 방어기전 연구 및 종오리백신 프로그램의 개발로 질병방어 및 제어를 목표로 연구를 진행하였다. 첫 번째, 국내에는 1형과 3형이 동시 유행하고 있으며, 최근에는 3형의 발생보고가 증가하는 추세이다. 특히 1형과 3형의 복합감염사례도 빈번히 발생하고 있다. 이런 상황에서 효율적인 질병관리를 위해 국내에 발생하는 오리간염 1형과 3형을 동시에 방어하는 혼합백신을 개발하고 그 효능을 확인하였으며, 운반 및 보관 용이성 향상을 위한 연구를 진행하였다. 두 번째, 오리감염 생백신의 3-5일 만에 조기면역을 획득하는 특성분석을 위해 세포성 및 체액성 면역반응을 평가하였다. Cytokine이 백신접종 그룹에서 증가하는 것을 확인하였고, 특히 IFN-α에서 강한 증가를 보였다. 또한 항체가 평가에서는 기존에 알려진 바와는 다르게 백신 후 3일째에 항체를 획득하였고, 10일째에는 최고수준의 항체가 수준을 나타냈다. 세 번째, 1주령이하의 어린 일령에 치명적인 질병의 특성상 모체이행항체에 의한 예방이 가장 효과적이라는 점에 착안하여 종오리백신 프로그램을 개발하고 농장에 적용하여 평가하였다. 종오리 농장 4개동 4천수의 종오리를 대상으로 백신을 실시하였으며, 모체의중화항체가(VN titer) 8 log2이상에서 후대병아리 14일령까지 100% 방어효능을 확인하였다. 모체이행항체의 전달률은 11%수준으로 나타났으며, 후대병아리의 항체가를 개체별 추적하여 계산한 결과 모체이행항체 반감기는 3-5일령임을 확인하였다. 본 연구에서는 오리에 치명적인 질병을 일으키는 오리바이러스성간염 예방을 위한 혼합생백신 개발에 관한 연구로 기존의 국내 상용화백신의 한계점을 극복하고, 국내에 유행하는 혈청형들을 단회백신으로 효과적으로 방어할 수 있는 혼합생백신을 개발하였으며, 백신의 특성상 조기면역에 대한 세포성 및 체액성 면역기전을 평가하였다. 또한 종오리백신 프로그램을 국내에 적용하여 모체이행항체에 의한 후대 병아리의 우수한 방어 효능을 확인하였다.

강황과 울금 항염중 효과 연구

김영훈, 배덕성, 김정화, 윤현정, 남인영, 김민선, 신희찬, 차현정, 황방연, 이재권 충북대학교 교육개발연구소 2011 한국교육논총 Vol.32 No.3

-본문참고-

최신 Single Nucleotide Polymorphism (SNP) 분석법의 원리 및 유전자 연구 응용법

차희재 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.3

This mini review describes the principles, protocols, and applications of three commercially available Single Nucleotide Polymorphism (SNP) genotyping platforms, the TaqMan® SNP Genotyping Assay, SNPlexTM Genotyping System and Gene Chip Assay. These technologies meet the requirements of multiple SNP applications in genetics research including diseases based on genetic background. This article also describes a set of SNP selection guide and useful web sites for SNP informations. Overall, the TaqMan assay format is suitable for low- to mid-throughput applications in which a high assay conversion rate, simple assay workflow, and low cost of automation are desirable. The SNPlex Genotyping System, on the other hand, is well suited for SNP applications in which throughput and cost-efficiency are essential, e.g., applications requiring either the testing of large numbers of SNPs and samples, or the flexibility to select various SNP subsets. Gene Chip Assay is powerful tool to analyze whole SNPs and has benefit to find new SNPs involved diseases or phenotype but has a defect that it is costly. In the conclusion, this article suggests the appropriate methods for the desired experiments by comparing with these three SNPs technologies.

흰쥐에서 paraquat독성에 미치는 tumor necrosis factor-α의 영향

차종희,박정수,양성훈,박재윤,이병래 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background : The toxicity of paraquat is due to the oxygen-derived radicals formed by the reaction of oxygen with bipyridylium radical cations. Tumor necrosis factor (TNF)-α induces the synthesis of mitochondrial manganese superoxide dismutase (Mn-SOD) and other protective proteins in a variety of tumor cells and these protein contribute to a protect tumor cells from free radical injury. In this study, the effects of TNF-α on paraquat-induced toxicity were investigated in rats. Methods : Experimental animal used was Sprague-Dawley male rat, body weight 150-200g. The paraquat toxicity was determined by survival rate. The SOD, catalse, and glutathione peroxidase(GSHPx) activity were spectrophtomerically determined by Crapo method, Aebi method and Flohe method, respectively. Results : The survival rate of paraquat-intoxicated rats was decreased by subctaneous injection of TNF-α. However, the survival rate of paraquat injected rats were increased by pretreatment of TNF-α, comparing to saline pretreatment. The liver SOD activity was increased significantly by TNF-α treatment, but kidney and lung enzyme was unaltered in TNF-α treated-rats, comparing to normal control rats. The increment of liver SOD activity by TNF-α treatment was dose- and time-dependant SOD activity of kidney and lung of rats were not changed by TNF-α injection. The activities of catalase and glutathione peroxidase and contents of thiobarbituric acid reactive substance(TBARS) in liver were not changed by TNF-α treatment. Conclusion : From these result, TNF-α potentiate paraquat toxicity in rats but TNF-α pretreatment reduce paraquat induced toxicity. Protective effect may be resulted from the increased liver SOD activities by TNF-α.

Haemophilus aphrophilus 심내막염 1예

차충환,신희봉,장성수,김민경,김양수,송재관,김미나 대한임상미샐물학회 2003 Annals of clinical microbiology Vol.6 No.2

집적 결상계의 기본 영상을 이용한 연속 시점 이차원 영상의 합성

박세희,장재영,서장일,차성도,신승호 강원대학교 정보통신연구소 2004 정보통신논문지 Vol.8 No.-

In this paper, we have studied continuous parallax two-dimensional(2D) image composition by use of elemental images which are picked up in integral imaging system. The 2D image composition method has been analyzed in terms of geometrical optics, and extended to the orthoscopic reconstruction. From the proposed composition method, it has been shown that the 2D image can be composed with arbitrary perspective and focus.

기호학적 접근을 통한 웹 페이지 이해 과정 탐색 : 화학교육 홈페이지를 중심으로

한재영,김은혜,차정호,노태희 경북대학교 사범대학부속 중등교육연구소 2007 중등교육연구 Vol.55 No.1

오렌지 껍질로부터 분리된 프라보노이드의 화학요법 감작효과

최철익,변익건,차윤정,김선필,민영돈,유진철,송재경 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : The overexpression of P-glycoprotein (Pgp) or multidrug resistanceassociated protein (MRP) confers multidrug resistance (MDR) to cancer cells. MDR cells could be sensitized to anticancer drugs when treated concomitantly with a chemosensitizer. In this study, orange peel have been screened for the development of chemosensitizers reversing MDR. Materials and methods : Expression of Pgp in AML-2/WT and AML-2/D100 were determined using western blot analysis and RT-PCR. Candidates for chemosensitizers were isolated, purified from orange peel using sephadex LH-20 column chromatography and silica-gel column chromatography, whose structure was determined using ^1H-NMR, ^13C-NMR, ^1H-^1H-COSY, 13C-NMR, EI-Mass, IR. Chemosensitizing effect of NP-28 on AML-2/DX100 over expressing Pgp and MRP was determined by the MTT assay. Membrane toxicity was evaluated by the RBC hemolysis test. Antioxidant effects were examined by using a fluorescence probe (2’-7’-dichlorofluorescein diacetate). Results : A potent candidate, NP-28, has a flavonoid nucleus with some methoxy moiety. NP-28 is believed to have high therapeutic index, being non-transportable inhibitor and possibly, of inhibiting other efflux pumps such as MRP and antioxidant effects. NP-28 is also unlikely to be a substrate of Pgp. NP-28 did not increase Pgp levels. Conclusion : NP-28 has a Chemosensitizing effect to reverse Pgp-mediated MDR by increasing the intracellular accumulation of drugs. Furthermore, they do not increase expression of Pgp, which can allow a long term use without the concern about Pgp activation. NP-28 is anticipated as an ideal second-generation flavonoid chemosensitizer and/or possibly other therapeutic agents by further investigation.

TMV에 의한 고추의 역병에 대한 전신획득저항성 유도

이성희,차재순,이주연 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1997 연구보고서 Vol.2 No.-

고추에 있어서 전신획득저항성 및 PR 단백질 축적과의 관계를 알아보기 위하여 TMV pepper strain을 고추잎에 접종하고 4일후 고추역병균 P. capsici를 고추줄기에 접종후 역병발생량을 비교하였다. TMV를 전접종한 고추에서는 TMV를 전접종하지 않은 고추에서보다 역병발생량이 크게 감소하였는데, TMV를 전접종한 고추의 역병 발병도와 병반길이는 역병접종 10일째에 TMV를 전접종하지 않은 고추의 약 50% 수준이었다. 담배의 PR1b와 PR5a 항혈청을 이용한 immuno-blot으로 추적한 고추의 PR1 과 PR5 단백질은 TMV 접종엽과 접종상위엽에서 TMV 접종 24시간후부터 세포간극에서 동시에 검출되기 시작하였다. PR1 과 PR5 단백질은 TMV 접종엽에서는 TMV 접종 4-5일후 잎이 탈락할때까지 그 양이 크게 증가하였다. PR5는 TMV 접종상위엽에서 TMV 접종후 그 양이 크게 증가하지는 않았지만 실험종료일인 TMV 접종 20일후까지 검출되었다. 이러한 결과는 고추에서 TMV 검염에 의해 역병에 대한 전신획득저항성이 유도되며, 전신획득저항성이 유도되는 동안 매우 빠르게 고추전신에서 PR 단백질의 생산이 유도되고, 생성된 PR 단백질들이 오래동안 유지됨을 암시하고 있다. Induction of systemic acquired resistance (SAR) against phytophthora blight and PR protein accumulation by TMV in pepper plant (Capsicum annuum cv. Nockwang) were examined to understand the mechanism of systemic acquired resistance in pepper plant. The zoospore suspension of Phytophthora capsici was inoculated on stem of pepper plant on which TMV-pepper strain had been inoculated on fully expanded 3 leaves, and the amount of phytophthora blight incidence was determined with time course. Both disease severity and lesion length of phytophthora blight were much smaller in TMV pre-inoculated pepper plant than in uninoculated control plant. The amount of phytophthora blight in TMV pre-inoculated pepper was about 50% of uninoculated control plant's at 10 days after P. capsici inoculation. PR1 and PR5 protein were monitored in intercellular fluid of TMV inoculated and uninoculated upper leaves by immuno-blot with tobacco PR1b and PR5a antibody to find accumulation of PR proteins locally and systemically during induction of SAR. PR1 and PR5 were detected from 24 hours after TMV inoculation both TMV inoculated and uninoculated upper leaves, and increased rapidly in TMV inoculated leaves until th leaves were defoliated on 4-5 days after TMV inoculation. PR5 was not increased in uninoculated upper leaves as much as in TMV inoculated leaves, but it can be detected until 20 days after TMV inoculation. These results suggest that TMV infection induces SAR against phytophthora blight in pepper plant, and PR proteins is accumulated very rapidly after TMV infection systematically during induction of SAR and maintained for quite long time in pepper plant.

아조벤젠 분자(Disperse Red 1)를 포함한 측쇄형 고분자에서 광유도 복굴절의 실시간적 조사

신희득,주원제,오차환,송석호,김필수,한양규 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-

아조벤젠 그룹을 포함한 측쇄형 고분자 필름(PDR1)에서 광 유도된 이방성을 실시간적으로 조사하였다. 수평, 수직한 흡수율의 변화를 광 유도된 복굴절의 기록, 감쇠, 소거, 완화 과정에 따라 측정하여, 매질 내의 아조벤젠 분자의 정렬상태와 복굴절과의 관계를 광 이성화과정으로 설명하였다. 또한 기록빔의 세기를 따른 수직, 수평한 흡수율의 변화와 유도된 복굴절의 관계를 조사하였다. The photo-induced anisotropy for a polymeric film(PDR1) containing azobenzene group as side-chain was investigated dynamically. The change of absrobances with parallel and perpendicular components was measured in recording, decaying, erasing and relaxation processes of photo-induced birefringence, so the relation between the alignment of azobenzene groups and the birefringence was well explained according to the photo-isomerization. Also, the dependence of the photo-induced birefringence on intensity of recording beam was investigated with changes of both absorbance and birefringence.