절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.

인체 지방 및 간장조직 중 유기염소제류 및 PCB의 확인 및 정량기법 체계화 : 2차년도

이상기,양자열,김기욱,이수연,오승민,김대현,정규혁 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

인체 지방 및 간장조직 중 유기염소제류의 확인 및 정량기법 체계화

이상기,양자열,인상환,김기욱,이수연,오승민,김미영,정규혁 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

Chitosan, PVA, 그리고 Chitosan/PVA 피막의 특성과 사과 겹무늬썩음병균 Botryosphaeria dothidea의 포자분산 억제작용

이승지,박동찬,김이부,엄재열,이용현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Chitosan을 피막을 형성하여 포자분산을 억제하는 새로운 사과 겹무늬썩음병 방제법의 피막재로 활용코져 연구하였다. Chitosan 필름과 polyvinylalcohol(PVA)을 혼합한 chitosan/PVA 혼합필름을 제조하고 인장강도, 신장률, 점도 등 물성을 검토하였다. Chitosan/PVA 혼합필름이 chitosan 단독 필름보다 우수한 피막형성능을 보였다. FT-IR spectra 분석결과 chitosan과 PVA간에는 새로운 화학결합 형성은 관찰되지 않았다. Chitosan과 chitosan/PVA 필름은 Botryosphaeria dothidea에 대한 상당한 항진균활성을 보였다. Chitosan과 chitosan/PVA 필름은 생분해성평가 공시균주들에 의하여 생분해성을 보인 반면 PVA 단독필름은 생분해성을 나타내지 않았다. 위의 각종 피막을 이용하여 과수원에서의 B. dothidea의 포자분산 억제효과를 측정한 결과, chitosan/PVA 혼합피막이 우수한 피막형성능과 피막지속성을 보였고, 포자분산 억제효과도 가장 우수하였으며, 1%의 chitosan과 5%의 PVA를 혼합한 피막의 경우 포자분산 억제률은 97.0%에 달하였다. The applicability of chitosan, a biodegradable natural polymer, as the coating material to prevent the dispersal of the spores of the apple white rot agent Botryosphaeria dothidea, was investigated. The physical properties of mixed chitosan/polyvinyl alcohol(PVA) film showed the increased physical properties for film formation, such as tensil strength, elongation, and viscosity, compare to either chitosan or PVA film. The FT-IR spectra of chitosan/PVA film indicated that the film was formed by simple blending not by any new synthetic bond. The chitosan and chitosan/PVA film showed effective antifungal activity on B. dothidea. The formed films were well decomposed by ASTM strains used for biodegradability test, on the other hand, the PVA film could not be decomposed by above standard strains. The field test at apple orchard showed that the dispersal of B. dothidea spores could be effectively reduced by coated film, especially by chitosan/PVA film. The spore dispersal was reduced upto 97.0% by 1.0% chitosan/5.0% PVA film during 4 months.

Iontophoresis 를 이용한 NADPH 의 피부투과 및피부자극

이승연,오승열 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.20 No.-

Nicotinamide Adenine Dinucleotide Phosphate (NADPH) has antioxidant effect in the body and thus decreases the symptoms of senility. The delivery of NADPH through skin is a formidable challenge due to its physical and chemical properties (such as rapid decomposition by oxidation, low partition coefficient in stratum corneum, high molecular weight and charge). The purpose of this work is to prepare a hydrogel patch system containing NADPH, and to study its in-vitro iontophoretic transport through hairless mouse skin. We have investigated two factors (concentration of NADPH and current density) that can affect the iontophoretic flux. The stability of NADPH in aqueous solution and the possible skin irritation by iontophoretic delivery of NADPH was also investigated. The results showed that, compared to passive flux, the total amount of NADPH transported increased by the application of cathodal current. Anodal flux was similar to passive flux. Flux increase was proportional to the concentration of NADPH in the donor solution and to the current density. Nearly 50 % of NADPH degraded during the experimental period in aqueous solution. The results of primary irritatation test showed no erythema or edema after iontophorsis. These results suggest that therapeutic amount of NADPH can be delivered through skin using iontophoresis, without any development of erythema or edema.

급성 단순하부요로감염의 경험적 치료로써 레보플록사신 3일요법의 효과

이지열,이승주,이상돈,정희창,오봉렬,김세웅,최한용,조용현,윤문수 대한화학요법학회 2001 대한화학요법학회지 Vol.19 No.4

목적 : 급성 단순요로감염의 원인균에 대한 내성증가에 따른 경험적 1차치료의 선택에 대한 어려움으로 인하여 최근 미국의 Infectious Disease Society of America(IDSA)에서는 이에 대한 가이드라인을 제시하였다. IDSA의 가이드라인에 따르면 그 지역의 TMP-SMX에 대한 내성균주의 비율이 10-20% 이상일 때는 급성 단순요로감염의 경험적 1차치료제로 플루오로퀴놀론제를 선택하도록 하였다. 이에 저자들은 급성 단순하부요로감염 환자에 있어서 레보플록사신에 대한 임상적, 미생물학적 유효성 및 안전성을 알아보고자 하였다. 방법 : 시험은 전향적, 비맹검, 공개, 비비교, 다기관 임상시험으로 시행되었고 전국 5개 대학병원을 방문한 56명의 급성 단순하부요로감염 환자에게 레보플록사신 100㎎을 1일 3회, 3일간 경구 투여하여 1-3일 후에 추적 관찰하였다. 결과 : 평가가 가능하였던 53명의 환자 중 50명의 환자에서 치료 후 5-7일 후에 증상이 소실되었거나 호전되어 94%의 임상적 유효성을 보였으며, 51명의 환자에서 치료 후 1-3일 후에 원인균이 제거되어 96%의 미생물학적 유효성을 보였다. 약물 관련 이상반응은 2%의 환자에서 나타났으나 이로 인한 치료 중단 사례는 없었으며 심각한 생화학적 또는 혈액학적 이상반응은 나타나지 않았다. 결론 : 레보플록사신 100㎎ 1일 3회 3일 요법은 임상적, 미생물학적 유효성이 뒤어나며 안전한 치료요법으로 급성 단순하부요로감염의 경험적 1차 치료에 적합할 것으로 생각된다. Background : The increasing frequency of clinical failure of uncomplicated urinary tract infections (UTIs) may be due to emerging resistance to commonly prescribed antimicrobials. Infectious Diseases Society of America guidelines state that uncomplicated UTIs should be treated empirically with a fluoroquinolone, if the proportion of trimethoprim/sulfamethoxazole-resistant isolates in the community is >10%-20%. The clinical efficacy and safety of levofloxacin were assessed in patients with acute, uncomplicated lower urinary tract infection. Methods : The study was a multicenter, prospective, open label, non-comparative, non-blinded trial. Fifty-six patients with acute, uncomplicated symptomatic lower urinary tract infections from five university hospitals were treated with levofloxacin 100㎎ tid for 3 days and were followed up for 1 to 5 days. Results : Fifty-three of the 56 patients were evaluable. Ninety-four per cent of patients were symptomatically cured or improved by the fifth to seventh day after therapy started. Bacteriological eradication of initial pathogen was achieved by 1 to 3 days in 96%. Drug related adverse experiences were seen in 2% of patients. None necessitated cessation of therapy. No significant biochemical or hematological abnormalities occurred. Conclusion : These results demonstrate the clinical efficacy and safety of empirical 3-day levofloxacin for acute, uncomplicated urinary tract infections.

치성감염에 의해 발생된 후측인두부 및 종격동 농양

이승호,정종철,김건중,정주성,유선열,박문성,김창룡 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Odontogenic infections are usually locally confined, self-limiting processes. However, under certain circumstances, they may break through the bony, muscular, and mucosal barriers and spread into contiguous fascial spaces or planes far from the initial site of involvement, resulting in severn life-threatening complications, such as retropharyngeal spread, suppurative mediastinal extension, airway obstruction, pleuropulmonary suppuration, and hematogenous dissemination to distant organs. The mortality arte for mediastinitis from odontogenic infection ranges from 40% to 60%. Therefore rapid evalution and treatment is essential with a combination of life support, antibiotic therapy, and surgical intervention. Recently, we experienced three cases of retropharyngeal and mediastinal abscesses secondary to odonogenic infections. In all patients, early diagnosis was possible by CT scanning and physical examination. The prognosis was good in all patients by using urgent aggressive surgical and antibiotic therapy.

연구개 및 구개수에 발생한 편평세포암종

이승호,이성훈,김기영,김창룡,박문성,류선열,조규승 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Carcinoma of the mouth accounts for approximately 5% of all carcinomas occurring in man. Carcinoma of the oral cavity develops as a result of invasion of malignant epithelial cells through the normally intact basal cell layer into subcutaneous and submucosal tissuse. The sofe palate and uvula may be involved in oral cancer but are not common sites. Early lesions of soft palate carcinoma appear as red, white, or mixed changes in the mucosa. The earliest symptom is mild sore throat. Advanced lesions interfer with swallowing and may cause a voice change. Although surgical method of soft palate carcinoma is successful, prognosis is relatively poor to swallowing and speech problem. Occasionally marginal recurrence may be developed. This article reports a case of squamous cell carcinoma occurred unusually in the soft palate and uvula. The case was treated with neoadjuvant chemotherapy, local radicalexcision and postoperative irradiation. Patient was followed up for 2 years. There was no tumor recurrence. The overall result including function was satisfactory.

이온토포레시스에 의한 경피 투과도 조절

이승연,여지선,김혜지,민혜란,오승열 숙명여자대학교 약학연구소 2004 약학논문집-숙명여자대학교 Vol.21 No.-

We have prepared hydrophilic karaya gum patches containing ketoprofen and studied some important factors which affect the transdermal flux of ketoprofen, as a first step to develop an iontophoretic transdermal patch system. The effect of penetration enhancer (propylene glycol monolaurate: PGML), short-time current treatment and continuous current on flux was studied. The effect of pH of the receptor solution and poly (L-lysine) (PLL) was also studied. PGML increased the passive flux markedly, and as the concentration of PGML in the matrix increased, passive flux increased. Iontophoretic treatment of skin (0.4 mA/cm²) for an hour increased the average passive flux more than two times, when the matrix contained no enhancer. It also increased the average passive flux further, when enhancer was incorporated into the matrix. Continuous iontophoretic transport showed unexpected result; the average flux at 0.2 mA/cm² was similar to that at 0.4 mA/cm², and was higher than that at 0.6 mA/cm². PLL increased the continuous iontophoretic transport at all current density, and the average flux was higher at 0.6 mA/cm² than at 0.4 and 0.2 mA/cm². Similar results were obtained when the receptor solution was changed to pH 4.0 buffer solution. These result demonstrate, that electro-osmotic flow is playing an important role in the flux of ketoprofen.

Chitosan이 사과 겹무늬썩음병균 Botryosphaeria dothidea의 생육에 미치는 영향

이승지,엄재열,이용현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

고분자물질에 의한 사과 겹무늬썩음병 방제법을 개발함에 있어서 chitosan을 코팅용 소재로 활용하기 위하여, 사과 겹무늬썩음병균인 Botryosphaeria dothidea에 대한 Chitosan의 항진균활성을 고체배양 및 액체배양 조건하에서 검토하였다. Chitosan은 고체배양시 B. dothidea의 균사생장억제, 자라난 균사의 응집, 균사의 팽윤 및 세포의 미세구조의 변화를 유발하였다. 액체배양실험 결과 chitosan은 낮은 농도에서도 강력한 균체생육 억제효과를 보였으며, 1.0mg/ml의 첨가농도에서 90% 이상의 억제효과를 나타내었다. 또한 균사의 신장과정에 영향을 미쳐 균사체의 응집현상이 관찰되었다. 또한 chitosan이 포자의 발아를 지연시켰을 뿐만 아니라 발아관의 형태적 변화를 유발시켰다. 단량체인 glucosamine은 glucosamine은 B. dothidea의 생육저해를 유발하지 않았으며, 항진균 활성은 chitosan polymer에 의한 것임을 알았다. To examine the potential utilization of chitosan, the biodegradable natural ploymer, as a control agent of apple white rot caused by Botryosphaeria dothidea in a new control measure by coating it on the diseased branches, the various antifungal activites of chitosan was investigated. Chitosan showed significant inhibitory effect on the mycelial growth of B. dothidea, along with the morphological changes including hyphal swelling and ultrastructural changes on solid PDA medium. In liquid PD broth medium, the chitosan showed more significant effect on the growth of B. dothidea also forming cell clusters indicating affection on the hyphal extension. The growth of B. dothidea was inhibited more than 90% at the concentration of 1.0 mg/ml. Chitosan also detained the spore germination and induced the morphological change of germ tubes. Glucosamine, monomer of chitosan, did not affect on the growth of B. dothidea indicating the antifungal activity was caused by chitosan polymer.