國內飼育 犬에 있어서 心臟絲狀蟲의 感染率에 관한 調査 硏究

Ji Hee Seong(成知禧),Hwa Joong Yoon(尹和重),Won Chang Lee(李元暢),Kwang Ho Kim(金光浩),Seung Won Kang(姜承遠) 한국예방수의학회 1996 예방수의학회지 Vol.20 No.2

The purpose of this study was carried out an epidemiological survey on the prevalence rate of the canine heartworms in Seoul City and the districts of Pa-Ju, Seong-Nam and Po-Chon in Kyonggi province of Korea from September 1994 to the end of September 1995. The blood samples from 292 male and 193 female dogs (pet, 363 ; fighting dogs, 60 ; working dogs, 62) were examined for the adult heartworms cuticle antibodies using the enzyme-linked immunosorbent assay (ELSA) kits. The test was designed to detect the antibodies of the host against the specific cuticle antigens produced with the adult heartworms.

내시경적 점막절제술로 치험한 식도 과립상 세포종 1례

강혁주,김성욱,최석진,이중현,장재식,서영범,윤병구,박건욱,김성자,김용섭,강승완,이구,양창헌,이창우,김욱년,이광헌,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

과립상 세포종은 Schwann 세포 기원으로 생각되며 인체에 비교적 드물게 발생한다. 과립상 세포종은 전신 어느 곳에서나 발견될 수 있으나 주로 혀, 구강, 피부 혹은 유방 등에서 호발하며 드물게 위장관에서 발견된다. 위장관에서는 식도에서 가장 호발하며 다음으로 위, 대장 순이다. 과립상 세포종은 대부분, 특히 위장관에서는 양성이며 소수의 악성 병변이 보고되었다. 이러한 이유와 함께 수술 전의 진단이 어렵기 때문에 과립상 세포종에 대한 근본적인 치료는 현재까지 외과적 절제술이다. 최근에 시도되는 치료방법들로는 내시경적 레이저 치료, 용종절제술, 내시경적 점막 절제술 등이 있다. 저자들은 상부 소화관 내시경검사를 시행하여 식도 과립상 세포종을 진단하고 내시경적 점막 절제술을 시행하여 합병증 없이 퇴원하여 현재 재발없이 경과 관찰중인 1례를 경험하였기에 보고하는 바이다. Granular cell tumors, which occur infrequently, are probably of Schwann cell origin. They can occur almost anywhere in the body but usually affect the tongue, oral cavity, skin, or breasts and are rarely found in the gastrointestinal tracts. The esophagus is the most frequent gastrointestinal site, followed by the stomach and the colon. Granular cell tumors are generally benign, especially in the gastrointestinal tract, some malignant lesions have been reported. For this reason, and also because preoperative diagnosis is difficult, the standard treatment for granular cell tumor has until now been surgical excision. In recent years, other therapeutic methods is endoscopic laser therapy (ELT), polypectiomy, endoscopic mucosal resection (EMR). We report a case of esophageal granular cell tumor which was diagnosed by an endoscopy and managed using an endoscopic mucosal resection without complication.

난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화

이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3

Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.

Junctional Rhythm을 보인 한국형 출혈열 1례

이성원,이수훈,최대현,손영기,차광수,손춘희,정원태,김성은,김종성 동아대학교의과대학 1997 동아의대학술지 Vol.9 No.1

초등학교 수준별 교육과정 운영 방안에 관한 연구

이성영,김광명,신준식 춘천교육대학교 초등교육연구소 2001 敎育硏究 Vol.18 No.-

무방류식 자연발효화장실에서 배양가능한 혐기성 세균

이관복,신광수,박성주 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.1

혐기성 소화 방식으로 분뇨를 처리하는 무방류식 자연발효화장실의 혐기성 세균을 연구하기 위하여 모형 화장실을 제작 가동하면서 혐기성 소화가 일어나는 발효조의 분뇨를 채취하여 모두 19개의 혐기성 세균 균주를 순수 배양하였다. 이들에 대하여 생화학적 특성을 조사하였으며 이를 기초로 한 표현형적 동정과 이와 동시에 165 rRNA 유전자의 염기서열 분석으로 동정을 실시하였다. 19개의 분리균주 가운데 3개는 분뇨의 주성분인 탄수화물 분해능이 거의 없었으며, 8개는 15℃에서도 생장 가능하였다. 표현형 및 유전자 동정이 모두 실시된 10개의 균주가 동일한 동정 결과가 나타난 것은 하나도 없었다. 유전자 동정 결과 9개가 Low G+C gram-positive bacteria 즉 Bacillus/Clostridium group에 속하였으며 이 가운데 8개가 Enterococcus 속이었다. The model human manure digestion tank without effluent was constructed and operated for studying anaerobic bacteria participating in anaerobic digestion of human manure. A total of 19 anaerobic bacterial pure isolates were obtained from human manure samples in the digestion chamber. Their phenotypic characteristics were examined by various biochemical tests, by which the isolates were identified. In addition. their genotppic identification were carried out by the 16s rDNA base sequence analysis. Three culturable isolates did not ferment carbohydrate compounds which are main constituents of human manure. Eight isolates grew at 15-C. None of ten showed the same identification results by phenotypic and genotypic tests. The 16s rDNA base sequence analyses showed that nine isolates of ten belong to Low G+C gram-positive bacteria, and eight of which belong to genus Enterococcus.

제7차 교육과정에 따른 산ㆍ염기 단원의 STS과학 교육에 적합한 CBL 실험 프로그램의 개발

이광필,김동화,최성호,류재정,이무상 慶北大學校 師範大學 科學敎育硏究所 2001 科學敎育硏究誌 Vol.25 No.-

This study developed the 'advanced-supplementary' course experimental program with CBL(Calculator-Based Laboratory) system in conformity with the reform of the 7th science curriculum and applied the CBL experimental program to 'acid-base' chapter of middle-high school. The results of this study shows that a practical use of CBL system as powerful instrument can be applied to experiment program of 'acid-base' chapter in order to causes learner's motivation and enlarge a sphere of inquiry-activity in science.

Shaphylococcus enterotoxin B와 lipopolysaccharide를 작용시킨 사람 섬유아 세포에서 생성된 Transforming Growth Factor-β₁의 정량적 분석

이성근,김광혁,김욱규,김종렬,정인교,양동규 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.2

TGF-β1 is a potent chemotactic factor for inflammatory cells and fibroblasts. It also stimulates the celluar source and components of extracellular matrix and the production of proteinase inhibitors. Collectively, these biologic activities lead to the accumulation and stabilization of the nascent matrix, which is vital to infection control. The objective of this study is to investigate production of TGF-β in vitro fibroblast culture in the presence of Staphylococcus enterotoxin B(SEB) and/or lipopolysaccharide(LPS) and to elucidate the role of TGF-β1 which may be responsible for infection control. The fibroblasts were originated from gingiva and facial dermis in 26 year-old male patient. In the presence of LPS(0.01㎍, 0.1㎍, 1.0㎍), SEB(0.01㎍, 0.l㎍, 1.0㎍) respectively, cells(5×103ml) were cultivated in vitro. At 1, 3, and 5 days after incubation, cells were counted. Also, cells(2.5×105ml) were cultivated in EMEM with LPS(0.01, 0.1 and 1.0㎍), SEB(0.01, 0.1 and 1.0㎍) respectively and LPS(0.1㎍) and SEB (0.1㎍) in combination for 24,48, and 72 hours respectively. Culture supernatants were harvested at 1, 2, and 3 days after incubation period and triplicate culture supernatants were pooled and TGF-β1 was assayed in duplicate. The results were as follows. 1.In gingival fibroblast induced with SEB and LPS respectively or in combination, the suppression of cell Proliferation occurred very significantly since 3 days after incubation, compared with the control and the production of TGF-β1 occurred very significantly at 1 day after incubation, compared with the control. 2.In facial dermal fibroblast induced with SEB and LPS respectively or in combination. the suppression of cell proliferation occurred very significantly at 1 day after incubation, compared with the control. In SEB exposure, the production of TGF-β1 was decreased very significantly at 1 day after incubation, compared with the control. However, in LPS, SEB and LPS exposure, the production of TGF-β1 was increased very significantly at 1 day after incubation, compared with the control. In conclusion, the concentration of bacterial toxins and the incubation period correlated with cell proliferation and production of TGF-β1 very significantly. The gingival and facial dermal fibroblasts have different phenotype each other The orchestrated understanding of fibroblast proliferation and TGF-β1 production play an important part in host defense against the bacterial Infection and may prevent tissue necrosis such as necrotizing fasciitis and life-threatening syndrome such as multiple organ failure.

國內飼育 犬에 있어서 心臟絲狀蟲의 感染率에 관한 調査 硏究

成知禧,尹和重,李元暢,金光浩,姜承遠 한국수의공중보건학회 1996 예방수의학회지 Vol.20 No.2

탐구적 과학수업에 대한 전북지역 과학교사의 인식 조사 연구

이광호,조규성,김수민 全北大學校 科學敎育硏究所 2000 과학과 과학교육 논문지 Vol.25 No.-

We studied aspects of science teachers in the Chonbuk province concerning the perceptions of inquiry science instruction using the some kinds of measuring tools. We applied the multiple comparison for twelve factors at the science teacher' backgrounds and analyzed the discrepancy in group levels. Science teachers understand the perceptions of inquiry science instruction a indifferent, but they don't feel self-satisfaction. We examined the tendency to the inquiry science instruction perceptions of science teachers having various backgrounds within 5 percent differences of p-value. It is proved that female teachers marked the higher scores than male teachers, public school teacher than private school teachers and young teachers in their twenties than older teachers. We have investigated the teachers just in Chonbuk province, but we should recognize that the scores in both the perception and the teachers are not improved comparing with the before-studies of Kim(1991). As a result, the development of measuring tools which teachers can examine themselves as their features is needed and the educati onal systems to specialize the science education should be improved through the various programs for teachers' training courses, the re-education and training for the present post.