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Mesenchymal stem cells (MSCs) are an attractive source for cell therapy, as they have the potential for differentiation into multi-lineage cells. Adipose tissue is a safe source due to its easy extraction and abundant resource, with minimal risk to the organ donor. In this study, we attempted to correlate the harvest yield and resulting multipotency of feline adipose tissue-derived mesenchymal stem cells (fAD-MSCs) in accordance with processing time. fAD-MSCs were individually isolated from the abdominal adipose tissues of 6 felines. They were divided into two groups, based on their processing times - Group 1: 0~1 day after adipose tissue harvesting; Group 2: more than 3 days after adipose tissue harvesting. In both groups, the proliferation capacity was analyzed using the cumulative population doubling level (CPDL) calculation assay. The expression levels of MSC-specific markers and differentiation potentials into mesodermal cell lineages were also evaluated. We observed that fAD-MSC isolation yields and CPDL were excellent in Group 1 compared with Group 2. We also found that the differentiation potential-specific genes (ACAN and OPN) were strongly expressed in Group 1 compared with Group 2. These results suggest that for the clinical treatments of feline diseases, fAD-MSCs should be isolated within 1 day after adipose tissue harvesting.
To evaluate the performance of a new automated coliform enumeration system (TEMPO(R) CC) for the quantitative test of coliform bacteria contaminated in domestic livestock processed foods, a total of 507 samples of livestock foods were tested by the TEMPO(R) CC method, the most probable number (MPN) method, and Petrifilm method, respectively. The results of those three methods were compared to each other. Of 507 samples of livestock processed foods used in this study, 217 samples were contaminated artificially with coliform bacteria and the rest (n=290) were contaminated naturally. The results of the TEMPO(R) CC method for all samples were equivalent to those obtained from the MPN method, except 8 samples. In addition, 496 (97.8%) out of 507 samples made agreement between the TEMPO(R) CC method and the Petrifilm method. The correlation coefficients between TEMPO(R) CC and MPN methods as well as between TEMPO(R) CC method and Petrifilm method were above 0.9, and the slope and intercept of the linear regression model was different in less than 1 value. In conclusion, there were statistically equivalent levels of performance between the TEMPO(R) CC and the reference and alternative methods for the enumeration of coliform bacteria in livestock processed foods in this study.
( Gyeong Been Lee ),( Jienny Lee ),( Yong Woo Sohn ),( Na Yeon Gu ),( Hee Ryang Kim ),( Jeong Su Byeon ),( Hyung Seon Jeon ),( Jong Duck Jang ),( Young Jin Yang ),( In Soo Cho ),( Sang Ho Cha ) 한국예방수의학회(구 한국수의공중보건학회) 2015 예방수의학회지 Vol.39 No.3
Bone fractures are most often seen in racetrack horses because of the high level of intensity in racing. These issues are the main cause of decreased performance in racehorses. Mesenchymal stem cells (MSCs) have been explored to improve intra-articular therapy in racehorses. MSCs are essential for the repair and regeneration of damaged tissues. In this study, the effect of intra-articular injection of MSCs in racehorses was investigated. Before accessing the MSC therapy, synovial fluids were obtained from the fracture site of racehorses, and adipose tissue was collected for MSC isolation. Using the MSC specific marker, adipose tissue-derived MSCs were identified. The racehorses received intra-articular injection of autologous MSCs (or allogeneic) (3 × 107 cells/3 mL). After 1 or 2 weeks, synovial fluids were collected from racehorses. To test the effect of MSC injection using ELISA, we analyzed inflammatory factors from the untreated samples compared to MSC-treated samples of racehorses. The level of pro-inflammatory factors (interleukin-1β and prostaglandin E2) was significantly decreased in synovial fluids of MSC-injected racehorses, compared to before accessing the MSC therapy, whereas, the level of anti-inflammatory factor (interleukin-10) was higher than prior to accessing the MSC therapy. Further studies are needed to investigate the anti-inflammatory mechanism of MSC in racehorses.
Mycotoxins, such as aflatoxin B1 (AFB1), deoxynivalenol (DON), fumonisin B1 (FMB1), ochratoxin A, T2 toxin, and zearalenone, are found in numerous vegetables. Mycotoxin accumulation in food and feed poses serious health risks to humans and animals because of carcinogenic, mutagenic, teratogenic, and toxic properties. In addition, mycotoxins cause large economic losses in commercial crop production, food and feed processing, and animal husbandry worldwide. In this study, an analytical method for the simultaneous analysis of the levels of AFB1, DON, and FMB1 in cow blood with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and validated. AOZTM and Myco6in1TM multitoxin immunoaffinity columns and an OasisTM reversed-phase solid-phase extraction Hydrophilic-Lipophilic-Balanced columns were used to purify and concentrate the blood samples. Extracts that contained AFB1, DON, and FMB1 had average recovery of 64.0%, 98.0%, and 89.9%, respectively. In conclusion, we used LC-MS/MS to detect several important toxicological mycotoxins in cow blood. The multimycotoxin method, which detected and quantified the levels of AFB1, DON, and FMB1 can be used in animal pilot studies to monitor simultaneous exposure to major mycotoxins.
In photobiophysics, biophoton means a kind of biological energy which enhances most metabolisms of the body. To investigate the immuno-enhancing effects of biophoton energy projector (BEP) producing light energy, pigs were irradiated with BEP for 8 weeks. Swine peripheral blood mononuclear cells (sPBMCs) were isolated from the blood of irradiated pigs. In this study, the antigen uptake and mitochondrial membrane potential of sPBMCs were measured by flow cytometric analysis. The irradiation of BEP increased the antigen uptake of sPBMCs. For functional analysis, the production of Bordetella bronchiseptica-specific IgG, measured using antigen-specific ELISA, was increased during the period of BEP irradiation. Taken together, the results suggest that the irradiation of BEP has immune-enhancing effects on sPBMCs.