백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

좌각차단을 동반한 심부전증 환자에 실시한 양심실 심박조율기 치료 : 증례 보고

김찬욱,차태준,김성만,김민대,권성일,서수홍,최병주,주승재,이재우 고신대학교 의학부 2002 高神大學校 醫學部 論文集 Vol.17 No.1

Epidemiological studies suggest that 30-50% of patients diagnosed with symptomatic congestive heart failure(CHF) have electrocardiographic evidence of a major intraventricular conduction delay, which may worsen left ventricular systolic and diastolic dysfunction through asynchronous ventricular contraction and paradoxical interventricular septal wall motion. Biventricular pacing is an emerging therapy allowing simultaneous electrical stimulation of the both ventricles with an implantable pacing system and it improves hemodynamics and well-being of patient by reducing ventricular asynchrony. Recently, we underwent biventricular DDD pacemaker implantation in a patient with CHF and Left bundle branch block and was significantly improved exercise tolerance and quality of life in that patient.

LFG에서의 統制理論

金玟柱 순천대학교 어학연구소 1988 어학연구 Vol.1 No.-

KIM, MIN-JOO(1988). Control theory in LFG, Language Studies, 1, I. The purpose of this article is to show that the control theory of LFG is more explanatory than that of GB. The basic assumption of this control theory is that grammatical functions such as SUBJ, OBJ, and OBJ 2, are primitives in grammatical relations and that grammatical functions are lexically encoded in predicate argument structures. Control referes to a relation of referential dependence between an unexpressed subject, 'PRO' and an expressed constituent, 'controller'. The control theory in GB does not solves some problems which occur in a specified sentence like a discource context out of a governing category. However, the control theory adopted here can make direct reference to grammatical functions. The property of being a SUBJ is a defining property of the functionally controlled element. LFG theory also assumes that the open function XCOMP and XADJ can denote controlled clauses. Our theory predicts that it will solve the problems using functional control and anaphoric control proposed by Bresnan.

糞尿와 都市 廢棄物과의 混合處理에 대한 硏究 (Ⅰ) : 肥料化를 中心으로한 實驗室的 考察 Experimental study for the fertilization

金熙鐘,金相烈,金秀生,孫晋彦,金燦國,成周慶,吳英敏 동아대학교 환경문제연구소 1977 硏究報告 Vol.1 No.1

우리나라에 있어서 都市廢棄物의 主인 糞尿와 固形廢棄物의 太宗인 無煙炭재를 同時에 效果的으로 處理할 수 있고 또한 堆肥化시킬 수 있는 好氣性 酸化方式의 最適條件을 얻기 위하여 生糞尿 및 煉炭재의 理化學的 및 生物學的 數値를 測定하고 이를 基礎로하여 上記 兩者를 混合處理 할수 있는 消化條件(溫度, 糞尿와 煉炭재의 配合比, 기타 固形物의 添加量, 注入되는 空氣量의 變化)에 따른 C.O.D., B.O.D.의 變化, 水分 및 電氣傳導度의 變化, 熱灼減量, N量의 變化 等을 硏究하였으며 이들 사이의 相關關係를 檢討하여 다음과 같은 結論을 얻었다. 1. 生糞尿에 固形廢棄物인 煉炭재나 紙類를 配合시켜 消化시키므로서 케이크속의 질소成分의 量을 增加 시킬수 있다. 2. 煉炭재의 配合은 糞尿의 消化速度를 增加 시킨다. 3. 糞尿의 處理를 위한 反應槽의 溫度는 50℃보다 60℃로 維持 시킬때 消化速度가 크다. 4. 反應槽의 溫度를 一定하게 했을때 空氣의 注入速度는 初期 反應時는 적게하고 後期 反應時는 크게 함이 效果的 이다. For the effective treatment and usable disposal of urban wastes in the area of Busan city, the composition of the nightsoil and anthracite bulky ash which is the main munic ipal solid wastes have been studied on the digestion of the compositive mixtures with the variable conditions that are ratio of compounding between nightsoil and ash, amount of solid additives and volume of air for the effective airation at 50, 65℃. The digestion has been detected by the physico-chemical and biological data; pH, specific gravity, content of moisture, C.O.D., B.O.D., electroconductance of the solution of the results, decrease of nition, and volume of total free nitrogen etc. The results are obtained as follows. 1) The characteristics of domestic nightsoil are different to the foreign data as table 5, but the data of anthracite ash almost are same except the composition of Fe₂O₃ and Al₂O₃. 2) Amount of total free nitrogen are increased by the digestion with the addition of solid waste, as effective absorption, to the nightsoil. 3) The digestion are accelerated by the compounding of ash, but the adding of sulfuric acid to fix the free ammonia reduced reaction. 4) The airation have to proceed slowly at initial step, and then endure enough volume of air at the later step.

Effects of Red Ginseng on Neonatal Hypoxia-induced Hyperacitivity Phenotype in Rats

Kim, Hee-Jin,Joo, So-Hyun,Choi, In-Ha,Kim, Pitna,Kim, Min-Kyoung,Park, Seung-Hwa,Cheong, Jae-Hoon,Shin, Chan-Young The Korean Society of Ginseng 2010 Journal of Ginseng Research Vol.34 No.1

Attention deficit hyperactivity disorder (ADHD) affects 4-12% of chool-age children worldwide and is characterized by three core symptoms: hyperactivity, inattention, and impulsivity. Although standard pharmacological treatments, such as methylphenidate and atomoxetine, are available, concerns about drug-induced psychological and cardiovascular problems, as well as growth retardation and sleep disturbances, highlight the continuing need for new therapeutic interventions. Using a neonatal hypoxia-induced hyperactivity model in rats, the potential positive role that oral administration of red ginseng extract may have in relation to the hyperactive phenotype was investigated. Hypoxia was induced in 2-day-old male Sprague-Dawley (SD) rat pups by placing them in a nitrogen chamber for 15 min. The neonatal hypoxia-induced rats showed a significant increase in hyperactivity phenotype, such as increased movement duration, movement distance, and rearing frequency, which was determined by monitoring their spontaneous locomotor activity using the Ethovision video tracking system. One week of oral treatment with red ginseng extract decreased the hyperactivity phenotype of the neonatal hypoxia-induced rats and increased the locomotor activity of the control rats. In the neonatal hypoxia-induced rats, expression of the norepinephrine transporter in the forebrain was increased, and red ginseng treatment partially prevented its up-regulation, while increasing its level in the control rats. Taken together, these results suggest that red ginseng extract decreased the neonatal hypoxia-induced hyperactivity phenotype, although it increased locomotor activity in normal animals.

3D Asthenopia in Horizontal Deviation

Kim, Seung-Hyun,Suh, Young-Woo,Yun, Cheol-Min,Yoo, Eun-Joo,Yeom, Ji-Hyun,Cho, Yoonae A. Informa Healthcare USA, Inc. 2013 Current eye research Vol.38 No.5

<P><I>Purpose:</I> This study was conducted to investigate the asthenopic symptoms in patients with exotropia and esotropia while watching stereoscopic 3D (S3D) television (TV).</P><P><I>Methods:</I> A total 77 subjects who more than 9 years of age were enrolled in this study. We divided them into three groups; Thirty-four patients with exodeviation (Exo group), 11 patients with esodeviation (Eso group) and 32 volunteers with normal binocular vision (control group). The S3D images were shown to all patients with S3D high-definition TV for a period of 20 min. Best corrected visual acuity, refractive errors, angle of strabismus, stereopsis test and history of strabismus surgery, were evaluated. After watching S3D TV for 20 min, a survey of subjective symptoms was conducted with a questionnaire to evaluate the degree of S3D perception and asthenopic symptoms such as headache, dizziness and ocular fatigue while watching 3D TV.</P><P><I>Results:</I> The mean amounts of deviation in the Exo group and Eso group were 11.2 PD and 7.73PD, respectively. Mean stereoacuity was 102.7 arc sec in the the Exo group and 1389.1 arc sec in the Eso group. In the control group, it was 41.9 arc sec. Twenty-nine patients in the Exo group showed excellent stereopsis (≤60 arc sec at near), but all 11 subjects of the Eso group showed 140 arc sec or worse and showed more decreased 3D perception than the Exo and the control group (<I>p</I> < 0.001, Kruskal-Wallis test). The Exo group reported more eye fatigue (<I>p</I> < 0.001, Kruskal-Wallis test) than the Eso and the control group. However, the scores of ocular fatigue in the patients who had undergone corrective surgery were less than in the patients who had not in the Exo group (<I>p</I> < 0.001, Kruskal-Wallis test) and the amount of exodeviation was not correlated with the asthenopic symptoms (dizziness, <I>r</I> = 0.034, <I>p</I> = 0.33; headache, <I>r</I> = 0.320, <I>p</I> = 0.119; eye fatigue, <I>r</I> = 0.135, <I>p</I> = 0.519, Spearman rank correlation test, respectively).</P><P><I>Conclusion:</I> Symptoms of 3D asthenopia were related to the presence of exodeviation but not to esodeviation. This may indicate that S3D symptoms are closely related to the convergence demand.</P>

A Rapid and Sensitive Two - Site Sandwich Enzyme - Linked Immunosorbent Assay for Detection of α - Fetoprotein in Human Serum

Kim, Hack Joo,Kim, Jeong Min,Jang, Jeong Su,Paik, Bo Hyun,Chung, Gi Hyun 생화학분자생물학회 1981 BMB Reports Vol.29 No.3

A rapid and sensitive method has been developed to detect α-fetoprotein (AFP) in human serum by a two-site sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (MAbs) for human AFP within 1 h. To obtain the most sensitive and reliable MAbs, 12 kinds of MAbs (HPJ1 to HPJ12) as a capture antibody and 4 kinds of horseradish peroxidase (HRP) conjugated MAbs as a tracer antibody were investigated. Among these, only HPJ 10-HRP conjugated HPJ 1 (HPJ 10-HPJ 1*) and HPJ 11-HRP conjugated HPJ 10 (HPJ 11-HPJ 10*) were chosen as candidates based on the linearity of the standard curve and the sensitivity of the assay. To further characterize these two pairs, MAbs against human AFP were purified from hybridoma cells, conjugated with HRP, and then characterized to optimize the two-site sandwich ELISA. The HPJ 10-HPJ 1 * pair showed a sensitivity of 1 ng/㎖ and a better reproducibility than the HPJ 11-HPJ 10* pair when the human sera were incubated at 37℃ for 30 min. The results obtained for 480 randomly selected human sera showed 0∼20 ng/㎖ of AFP values for the normal human sera. To test the utility of our kit, AFP concentrations were determined for 951 human sera (including 85 normal sera, 480 random blood sera, 213 HBsAg-positives, 50 anti-HCV antibody positives, and 47 malignant diseases) and compared with other commercially available AFP detecting kits. These results show that the present two-site sandwich ELISA method is a rapid, sensitive, and reliable procedure for detecting AFP in human serum.

Effect of Al and Mg Contents on Wettability and Reactivity of Molten Zn–Al–Mg Alloys on Steel Sheets Covered with MnO and SiO2 Layers

Joo‑Youl Huh,Min‑Je Hwang,Seung‑Woo Shim,Tae‑Chul Kim,Jong‑Sang Kim 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.6

The reactive wetting behaviors of molten Zn–Al–Mg alloys on MnO- and amorphous (a-) SiO2-covered steel sheets wereinvestigated by the sessile drop method, as a function of the Al and Mg contents in the alloys. The sessile drop tests werecarried out at 460 °C and the variation in the contact angles (θc) of alloys containing 0.2–2.5 wt% Al and 0–3.0 wt% Mg wasmonitored for 20 s. For all the alloys, the MnO-covered steel substrate exhibited reactive wetting whereas the a-SiO2-coveredsteel exhibited nonreactive, nonwetting (θc > 90°) behavior. The MnO layer was rapidly removed by Al and Mg contained inthe alloys. The wetting of the MnO-covered steel sheet significantly improved upon increasing the Mg content but decreasedupon increasing the Al content, indicating that the surface tension of the alloy droplet is the main factor controlling its wettability. Although the reactions of Al and Mg in molten alloys with the a-SiO2 layer were found to be sluggish, the wettabilityof Zn–Al–Mg alloys on the a-SiO2 layer improved upon increasing the Al and Mg contents. These results suggest that thewetting of advanced high-strength steel sheets, the surface oxide layer of which consists of a mixture of MnO and SiO2,withZn–Al–Mg alloys could be most effectively improved by increasing the Mg content of the alloys.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Alendronate의 뼈모세포를 통한 뼈파괴세포 형성 억제 기전

김현진,김민석,김태성,이은주,박하옥,김백윤,김선헌 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.6