대전광역 시민의 골다공증 검진에 관한 고찰

유은주,이관우,박현모,이수배,김광현,강희구,박성오 한국건강관리협회 1999 건협 Report Vol.2 No.-

Verticillium sp.가 생산하는 cellulase의 정제 및 그 성질

유주현,홍대희,양융,진효상 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

The cellulase of verticillium sp. was purified to investigate its some properties. The enzyme purification was carried out by ammonium sulfate fractionation, and column chromatography of Sephadex G-50, G-100 and DEAE Sephadex A-25, with its specific activity increased to 17 fold. The molecular weight was estimated to be 46,000 by gel filtration. The optimum pH and temperature were pH 7 and 50℃, respectively. The enzyme was stable over the range of pH5-7 at 50℃ for 1 hour. But half of its activity was lost after 2 hour. The km value was calculated to be 1.2mg of CMC/㎖.

열공형과 비열공형 피질하 혈관성 치매에서 위험인자의 차이에 관한 비교 연구

배희준,정지향,유경호,나덕렬,김상윤,최경규,양동원,손의주,이상도,김재우,박경원,김응규,이재홍,박미영,한일우,함동석,최문성,하충건,최성혜,이애영,이병철,한설희 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.2

Backgrounds and Objectives: Vascular dementia is a group of dementing disoders arising from various stroke syndrome. Among these. subcortical ischemic vascular dementia (SIVD) is regarded as a relatively distinct clinical entity. However, MRI patterns of SIVD are not homogenous. In some patients, lacunes are dominant, and in others, subcortical white matter changes are. This study was designed to compare risk factor profiles between SIVD with and without multiple lacunes. Methods: We divided 47 subjects (22 males, mean age. 68 years) recruited from VADAPET (Multicenter Trial For Evaluation Of The Changes In the PET Images Of Subcortical Vascular Dementia Patient) study into two groups one with more than 5 lacunes in deep gray matter (lacune group) and the other with 5 or less(non-lacune group) Clinical characteristics and laboratory findings of two groups were compared. Results: Nineteen of 47 patients (40%) belonged to the lacune group. The lacune and non-lacune groups d d not differ in the following variables: age, hypertension, diabetes mellitus, hyperlipidemia heart disease, history of stroke or TIA, history of trauma or major surgery, family history of hypertension stroke, or dementia, age at diagnosis of dementia, body mass index, white blood cell count, ESR, CRP, fibrinogen, hemoglobin A1C, total cholesterol. LDL cholesterol creatinine, proteinuria, glucosuria, and microhematuria. However, male sex, smoking alcohol. hemoglobin, and HDL cholesterol were possibly associated more with lacune group SIVD than with non-lacune group (p<0 1) Multivariate analyses revealed that smoking, hemoglobin, and HDL cholesterol were independent predictors of SIVD with multiple lacunes Conclusion: Our study suggests that SIVD with multiple lacunes may be significantly different in smoking habits hemoglobin, and HDL cholesterol from SIVD without multiple lacunes.

쌍태아에서 제 1태아의 분만 후 제 2태아의 지연분만 : 중례보고

김주환,서민정,유희정,노권일,조대현,박정규,이정헌,조성남,손영수 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.3

With the widespread use of fertility medications and techniques of in vitro fertilization, multiple gestation has become epidemic in modern obstetric practice. Delivery of the initial fetus in a multiple gestation usually is followed by delivery of the subsequent fetus or fetuses shortly thereafter. However in rare circumstances, the delivery is delayed for days due to disappearance of uterine contraction after delivery of the first fetus. When uterine activity ceases after the delivery of the first neonate, a policy of nonintervention may be considered in case of an immature or very premature delivery, in order to achieve a gestational age for the remaining fetus(es) more compatible with neonatal survival. We report a case of twin gestation with prolongation of the delivery interval between the twins for 46 days.

전문비서란 무엇인가? : Its Concept and Function

김주연,용주현,신동희,유민,최정윤 이화여자대학교 법정대학 비서학과 1995 비서학연구 Vol.- No.15

Acylthiocholine의 합성

정두희,이지현,박유미,정대일,곽문정,신영주 東亞大學校附設基礎科學硏究所 1998 基礎科學硏究論文集 Vol.15 No.2

Choline esters that are used with substrate of EE-AChE-catalyzed hydrolyses were synthesized by two methods. 2-Chloroethyl thiohexanoate 4a, 2-chloroethyl thioheptanoate 4c, and 2-chloroethyl thiooctanoate 4b were synthesized by treatment of hexanoyl chloride 3a, heptanoyl chloride 3c, and octanoyl chloride 3b with ethylene sulfide 2. Hexanoylthiocholine 6a and octanoylthiocholine 6b were synthesized by using 4a and 4b with trimethylamine 5. Secondly after reaction ethylene sulfide 2 with dimethyl amine 7 and acylation by acid anhydride 8, heptanoylthiocholine 6c, decanoylthiocholine 6d were synthesized by treatment of methyl iodide.

Dicyma sp. YCH-37이 생산하는 효모세포벽 용해효소 : 1. 생산균주의 분리 및 효소의 정제 l. Isolation of the Strain and Purification of the Enzyme

정희철,함병권,배동훈,하세가와토루,유주현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

효모세포벽 용해효소를 생산하는 균주를 토양으로부터 분리하였다. 본 균주에 대한 현미경 관찰 및 형태학적, 배양상의 특징을 검토한 결과 Dicyma sp.로 동정되어, 본 균주를 Dicyma sp YCH-37로 명명하였다. 세포에 아무런 처리도 가하지 않은 생균상태의 세균, 효모, 곰팡이에 대한 용균 spectrum을 측정한 결과, 세균 중에서는 Salmonella typhimurium을, 효모 중에서는 Torulopsis, Hansenula, Zygosaccharomyces rouxii 등을 잘 용해시켰으며, 가압증기살균 처리된 효모는 모두 용해시킬 수 있었다. 배양상등액을 yeast glucan을 이용한 affinity adsorption 및 DEAE-cellulose column chromatography로 정제한 결과, 13%의 수율과 204배의 정제도로 정제되었다. SDS-PAGE, gel filtration을 통해 분자량은 약 25,000 daltons임을 확인하였으며, 용균활성의 최적 pH와 온도는 각각 8.0과 50℃이고, pH 4.0~pH 10.0, 40℃까지 안정하였다. The strain YCH-37, which produces yeast cell wall lytic enzyme, was isolated from soil. From the microscopic observation, morphological and cultural characteristics, this strain was identified to fungus, Dicyma sp. So, we named this strain as Dicyma sp. YCH-37. The lytic enzyme effectvely lysed Salmonella typhimurium among intact living bacteria and Torulopsis, Hansenula, Zygosaccharomyces among intact living yeast, as well as autoclaved yeast strains. The yeast cell wall lytic enzyme was succesively purified to 204 folds with 13% yields through yeast glucan affinity adsorption and DEAE-cellulose column chromatography. The enzyme was identified to monomeric protein with molecular weight of 25,000 daltons from the results of SDS-PAGE and gel filtration. The optimum pH and temperature for the yeast lytic activity were 8.0 and 50℃, respectively. The enzyme was stale up to 40℃, and between pH 4.0~pH 10.0.

Cellulase생산을 위한 Verticillium sp.의 배양조건의 검토

홍대희,진효상,오두환,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

The cultivation conditions on wheat bran of verticillium sp. were optimized to produce cellulase as much as possible. The highest cellulase activity was shown, as verticillium sp. was cultivated at 27℃ for 5 days on wheat bran media with 1.2㎖/g wheat bran of water content and supplementation of NaNo and FeSo, in concentration of 0.1% and 0.2-0.3%, respectively.

하악 전돌증 환자의 구내 하악골 상행지 골절단술전후의 하악골 운동양상 및 저작근 근전도 변화에 관한 연

박영철,황충주,유형석,한희경 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.2

악구강계는 측두하악관절, 근육신경계, 치아, 그리고 지지조직의 복합적 구성체로 되어있어 다양한 구조를 이루고 있으며, 밀접한 생리적 관계를 유지하고 있다. 특히 하악은 저작근을 포함한 여러 근육을 운동원으로 하여 각종 기능을 수행하고 있으며, 이들 근기능의 활성도는 치근막을 비롯한 악관절과 근육 등의 여가 가지 형태의 수용기를 거쳐 말초 신경계와 중추신경계의 복잡한 경로와 기전에 의해서 조절되어지는데, 교정치료, 특히 교정을 동반한 악교정 수술시에는 이러한 악구강계의 변화와 구강내 여러감각 수용기의 변화, 근활성도의 변화가 심하게 나타나며, 이는 하악골의 기능변화를 초래하게 된다. 이에 저자는 최근 급증하는 악교정수술환자의 하악골 근육의 생리적 활성도나 하악골의 운동변화를 분석하여 보다 r객관적인 술전 및 술후의 평가기준을 제시하고자, Biopak을 이용하여 정상교합자와 하악전돌증을 주소로 내원하여 악교정수술을 시행받은 골격성 III급 부정교합자의 하악기능 양상을 총괄적으로 비교분석하여 다음과 같은 결과를 얻었다. 1. 각 군간의 근활성도 비교시 resting시에는 술전군이 정상군에 비해 더 큰 근활성도를 나타내었고, 수술후 그 측정치가 점차 감소함을 볼 수 있었다. clenching시에는 술전군의 교근과 전측두근의 근활성도가 정상군에 비해 더 감소되어 있었고 수술후 다시 증가하여 수술전과 술후 6개월군 사이에 통계적 유의차가 관찰되었다. 2. 각 근육 활성도 간의 비교시 resting시에는 전측두근의 활성도가 나머지 근육에 비해 다소 증가된 양상을 보였으나 통계적 유의차는 없었고, clenching시네는 모든 group에서 전측두근과 교근의 활성도가, swallowing시에는 악이복근의 활성도가 통계적 유의차를 보이며 크게 나타났다. 3. 하악운동범위의 비교시 전반적으로 술저군에서 제한된 운동 범위를 나타내었으며 최대개구량은 술후 6개월에, 전방 운동량은 술후 3개월에 유의성 있는 증가를 보였다. Stomatognathic system is a complex one that is composed of TMJ, neuromuscular system, teeth and connective tissue, and all its components are doing their parts to maintain their physiological relationships. Mandible, in particular, performs various functions such as mastication, speech, and deglutition; the muscular activities that determine such functions are signalled by numerous types of proprioceptors that exist in periodontal membrane, TMJ, and muscles to be controlled by complicated pathways and mechanics of peripheral and central nervous system. Orthodontic treatment, especially when accompanied by orthognathic surgery, brings dramatic changes of stomatognathic system such as intraoral proprioceptors and muscle activities and thus, changes in patterns of mandibular function result. The author tried to analyze changes in patterns of mandibular movement and physiologic activities of surrounding muscles in Skeletal Class III orthognathic surgery patients who presently show a great increase in numbers. The purpose of this study was to draw some objective guidelines in evaluation functional aspects of orthognathic surgery patients. Mandibular functional analysis using Biopak was performed for skeletal Class III prognathic patients who underwent IVRO(Intraoral Vertical Ramus Osteotmy), and the following results were obtained: 1. Resting EMG was greater in pre-surgical group than the control group, and it showed gradual decrease after the surgery. Clenching EMG of masseter and anterior temporalis of pre-surgical group was smaller than those of control group; they also increased post-surgically, and significant difference was found between pre-surgical and post-surgical(6 months) groups. 2. Resting EMG of anterior temporalis was greater than that of all the other muscles, but there was no significant difference. Clenching EMG of anterior temporalis and masseter were greater than those of the other muscles with statistical difference. In swallowing, digastric muscle showed the highest EMG with statistical significance. 3. Limited range of mandibular movement was shown in pre-surgical group. Significant increase in maximum mouth opening was observed six months post-surgically, and significant increase in protrusive movement was observed three months post-surgically.

Streptobacillus sp.가 생산하는 pullulanase에 관한 연구

오두환,지희숙,최형택,신원철,유주현 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.2

A bacterium with the highest pullulanase activity was isolated from the soil and identified as Streptobacillus sp. Y-716. The optimum culture condition of Streptobacillus sp. for the production of pullulanase was as follows; 1.2%(w/v)soluble starch, 0.7%(w/v) CH₃COONH₄, 0.05%(w/v) MgSO₄7 H₂O, 0.01%(w/v)FeSO₄7H₂O, at 30℃, for 48 hrs. The pullulanase from the culture broth of Streptobacillus sp. Y-716 was purified about 46 fold by ammonium sulfate precipitation, dialysis. DEAE-cellulose and Sephadex G-200 column chromatography. The purified enzyme was stable between pH 5.0 and pH 7.5. It was inactivated rapidly above 35℃. The Km value and Vmax of the purified enzyme were 0.0137mg/m/ and 0.666μm/min. on pullulan, respectively.