범가자미, Verasper variegatus의 난황단백질 분리 및 면역학적 특성

백혜자,한창희,김우진,박중연,김진한,김윤 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

The yolk protein of spotted flounder, Verasper variegatus was purified by precipitation with cold distilled water, followed by Sepharose CL-6B column chromatography. The purified protein was identified as vitellin by Ouchterlony's immunodiffusion test and immunoelectrophoresis. The purified vitellin from ovarian crude extracts has same antigenic determinants with the female specific serum protein, vitellogenin. The molecular weight of purified vitellin was estimated about 550kD by gel filtration. The vitellin was composed of three major subunits with molecular weight of about 108, 85 and 31 kD, and two minor subunits. The vitellin was identified by western blot analysis using anti-vitellin antibody.

구강상피세포의 냉동보관 방법에 따른 세포생존률 비교

백도영,이승종,정한성,김의성 대한치과보존학회 2009 Restorative Dentistry & Endodontics Vol.34 No.6

본 연구의 목적은 구강상피세포를 배양한후 각기 다른 조건의 냉동 보존법으로 6일간 보존시 각각의 세포의 활성도를 Cell counting, WST-1, Clonogenic capacity의 방법을 이용하여 비교 평가하기 위함이다. 각 실험군당 1×10^(6)개의 세포를 다음의 방법으로 6일간 냉동 보존한다. Freezing container에 담아 1℃/min의 냉동속도로 까지 냉동 후 -196℃에 냉동하여 보관한 일반 냉동 보존군, 세포를 바로 -196℃의 액화질소에 넣어 냉동한 급속 냉동 보존군, 4℃에서 -35℃까지 -0.5℃/min속도로 서서히 냉동시킨 뒤 -196℃에 냉동한 저속 냉동 보존군, 2 Mpa, 3 Mpa의 압력을 가하고 -0.5℃/min속도로 4℃에서 -35℃까지 서서히 냉동시킨 뒤 -196℃에 냉동한 2 Mpa, 3 Mpa압력 저속 냉동 보존군으로 나누었다. 6일 후 냉동되었던 세포를 급속 해빙하여 각각의 Cell counting, WST-1, Clonogenic capacity 값을 측정하여 비교하였다. 실험 결과 2 Mpa혹은 3 Mpa의 압력을 이용한 저속 냉동법이 저속 냉동법 및 급속 냉동법 보다 세포 활성도에 있어 우수한 경향을 나타내었다. This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting. WST-1, and clonogenic capacity values were measured and compared. 1. The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05). 2. The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 3. The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05).

흉부외상 없이 발생한 흉부대동맥 절단증의 조기 진단 : 2례 보고 Two Cases Report

한승백,전영진,백광제,김준식,김정택,김광호,선경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Aortic transection or interruption is a rare condition which developed after an acute deceleration injury. Its occurrence depends on the location and direction of the force applied and is usually from motor vehicle accident or falling down. The exact incidence of aortic transection in trauma is not known but, when develops, only about 10-15% of the victims can survive and be transported to the hospital. Even in the survivors, majority of them will be fatal within a few days if a prompt diagnosis and surgical treatments are not made. Aggressive diagnostic work-up is recommended for the patients with high suspicious index, which would salvage the victims with this fatal condition. We report the experience of two cases of aortic transection or interruption following motor vehicle accidents.

강원도 하천 호안 실태조사

최한규,정영덕,백효선 江原大學校 産業技術硏究所 2009 産業技術硏究 Vol.29 No.B

The propose of this study is to survey the revetments installed in Gangwon Province for identifying their characteristics. The survey showed that in eastern Gangwon Province, gabion, retaining wall and concrete blocks took about 63% or more, and in western Gangwon Province, dry wall, gabion and retaining wall, 68% or more. Gabion and dry wall had better vegetation coverage in western Gangwon Province than in eastern Gangwon Province. That is thought to be due to slope of river and under-water frequency.

레진 계열 근관봉함재 Adseal의 세포독성에 관한 연구

김희정,백승호,이우철,박한수,배광식 大韓齒科保存學會 2004 Restorative Dentistry & Endodontics Vol.29 No.6

The properties of ideal root canal sealers include the ability of sealing the total root canal system and no toxic effects to periradicular tissues. Cytotoxicity test using cell culture is a common screening method for evaluation of the biocompatibility of root canal sealers. The purpose of this study was to investigate the cytotoxic effect of newly developed resin-based sealer (Adseal 1, 2, and 3) comparing with those commercial resin-based sealers (AH26 and AH Plus), ZOE-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT) and calcium hydroxide based sealer (Sealapex). An indirect contact test of cytotoxicity by agar diffusion was performed according to the international standard ISO 10993-5. L929 fibroblast cells were incubated at 37℃ in humidified 5% CO₂-containing air atmosphere. The freshly mixed test materials were inserted into glass rings of internal diameter 5 ㎜ and height 5 ㎜ placed on the agar. After the 24 hrs incubation period, the decolorization zones around the test materials were assessed using an inverted microscope with a calibrated screen. A Decolorization Index was determined for each specimen. Adseal 1, 2, and 3 did not exert any cytotoxic effects, whereas AH26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, and Sealapex produced mild cytotoxicity.

결장직장 암종에서 Brain Type Glycogen phosphorylase 발현에 대한 면역조직화학적 연구

김수한,정동준,이정은,백무준,김창진 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

The colorectal carcinoma shows the highest incidence of cancer death in Korea. The colorectal carcinomas are diverse in their carcinogenesis, as some progress from polypoid adenoma to carcinoma while others occur de novo, or combined de novo and adenoma to carcinoma sequence. The searchs for the early biomarker of the colorectal carcinoma are important not only for the early detection of the cancer but also for the effective treatment. Brain type glycogen phosphorylase (BGP) is a kind of fetal isoenzyme frequently expressed in the various carcinoma, such as gastric carcinoma, renal cell carcinoma and colorectal carcinoma. In this study, the expression of BGP was evaluated in the colorectal carcinoma, normal appearing mucosa adjacent to the carcinoma and aberrant crypts foci (ACF) by immunohistochemistry using 70 cases of colorectal carcinoma. The results were as follows. 1. The BGP was expressed in 91.4% of 70 cases of colorectal carcinoma. 2. The expression rate of BGP accoarding to the location of the carcinoma was not different. 3. Seventeen percent of the colorectal carcinoma occurred by the polypoid adenoma-carcinoma sequence, while 60% of the cases occurred from de nove and 22.9% from combined polypoid adenoma-carcinoma sequence and de nove. 4. The BGP was expressed in 91.4% of normal mucosa adjacent to the carcinoma, while in 17.1% of normal mucosa for apart from the carcinoma. The BGP expressed in the above results suggest that the colorectal carcinoma arsie more frequently in de novo than polypoid adenoma-carcinoma sequence, which suggest that the pathogenesis of coloretal carcinoma in Korean might be different from that of western people. And the expression pattern of BGP in the colorectal mucosa could be early biomarker of carcinoma and the immunohistochemical stain for the expression of the BGP might be used for the early detection of the colorectal carcinoma.

비강비호즈킨 림프종의 전산화단층촬영 소견 : CT Features

노태연,백호길,원종부,박성호,박오봉,백승국,신미정,김봉기,최한용 침례병원 1998 浸禮病院學術誌 Vol.14 No.-

노인 인구에서 신경인지기능저하와 혈청 지질농도와의 상관성

유영선,최석주,정성수,김지은,윤성욱,전동욱,백준형,박성우,이정구,추일한,김영훈 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.6

Objectives : In this study, the authors evaluated the correlation between eurocognitive impairments and serum lipids levels among Korean elderly over the age of 65. Methods : A total of 609 elderly individuals participated in this study. Screening for cognitive impairments were carried out using the Mini-Mental Status Examination-Korean version (MMSE-KC). There were 197 subjects above 1.5SD Of MMSE- KC and they were evaluated using the Korean versions of the Consortium to Establish a Registry for Alzheimer's Disease (AD) (CERAD-KC) and Geriatric Depression Scale (GDS-K). Results : There was a correlation between old age/low levels of education and AD (p<0.05, P<0.01). There was an inverse correlation between the serum level of total cholesterol and the word list recognition test scores. The serum levels of LDL cholesterol were inversely correlated with the scores on the constructional praxis test, word list recall test and word list recognition test. Inverse correlations between the serum level of triglyceride and scores on the word list recall test and word list recognition test were also observed. Conclusion : There were inverse correlations between the serum levels of lipids and language/memory function in subjects with AD.

가축분뇨 비료성분 부하수준을 고려한 지역별 적정사육두수 설정

김재환,박치호,한정대,박백균 한국농업정책학회, 한국축산경영학회 2001 농업경영정책연구 Vol.28 No.2

In relation to pollution load, 166 towns and cities set the following optimum number of livestock, according to cultivated area, the amount of fertilizer ingredient for crops and wastes. According to the analysis of the total capacity for waste based on cultivated area and fertilizer requirement, N was 279,884 M/T, P2O5 140,464 M/T and K2O 182.559 M/T. Total amount of nutrient production according to the number of livestock were 222,331 M/T N, 64,716 M/T P2O5 and 86,670 M/T K2O, which are 86.7%, 53.6% and 52.7% of the fertilizer requirment. It is estimated that 45 regions showed an overload capacity of fertilizer per hectare in N, 11 regions in P2O5 and 13 regions in K2O, The converted optimum number of livestock was 13,415,000 Livestock Unit (LU).

國內 大氣質管理의 現況과 問題點

한명호,김차영,최정한,백성옥 嶺南大學校 環境問題硏究所 1991 環境硏究 Vol.11 No.1

본 논문에서는 국내에서 현재 법적인 규제가 행하여지고 있는 대가오염물질의 오염도 현황과 대기질 관리의 문제점을 여러가지 측면에서 서구의 선경험국가들의 경우와 비교 분석하였으며 이러한 문제점에 대한 대책을 강구하고자 하였다. 이와 아울러 현재 법적 규제의 범위에는 포함되어 있지는 않으나 잠재적 유해성이 큰 오염물질의 환경학적인 중요성과 현재의 관리실정 및 향후의 대책에 대하여 검토하였다. The current situation and inherent problems of air quality management in Korea were critically evaluated and compared with other developed countries, with respects to the ambient standards, air quality monitoring, emission inventory, and energy consumption patterns. Environmental implications and a worldwide trend in the management of non-criteria pollutants, was also discussed with a particular emphasis on the legislative aspects. .