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Production of -Steroids in Longchin Goby, Chasmichthys dolichognathus during Oocyte Maturation
백혜자 한국발생생물학회 2008 발생과 생식 Vol.12 No.1
점망둑의 난모세포 성숙과정에서 생성되는 주요 성 스테로이드 호르몬, -스테로이드를 분석하고자 전구물질 ()를 성숙기 난모세포(난경 ) 배양초기에 첨가하여 24시간 배양하였다. 스테로이드 대사물질 분석과 동정은 thin layer chromatography와 gaschromatography-mass spectrometry로 이루어졌다. 로부터 생성된 주요 성 스테로이드 대사물질은 -hydroxy, -dihydroprogesterone ()와 -hydrox To investigate the -steroids produced from maturating oocytes in the longchin goby, Chasmichthys dolichognathus, the oocytes () were incubated with radiolabeled -hydroxyprogesterone () for 24 hours. The resulting metabolites were analyzed by thin layer chromatography and identified by gas chromatography-mass spectrometry. Two -steroids, -hydroxy, -dihydroprogesterone () and -hydroxy, -dihydroprogesterone (), were converted from in the maturing oocytes. These two main metabolites were detected at 0.80 mm diameter oocytes or greater. In addition, the effects of these metabolites on in vitro germinal vesicle breakdown (GVBD) were tested. The sensitivity of oocytes to the induction of GVBD was greater at than . This result showed that is a major maturation inducing steroid (MIS) in longchin goby, suggesting may play a role in regulating the oocyte maturation process.
백혜자,황인준,이영돈,김형배 한국통합생물학회 2011 Animal cells and systems Vol.15 No.3
We investigated the in vitro effects of nonylphenol (NP) and 3,3',4,4',5-pentachlorobiphenyl (PCB126) on steroidogenesis in redlip mullet, Chelon haematocheilus, oocytes. In experiment 1, we investigated the effects of NP and PCB126 on steroid production from exogenous steroid precursors. Vitellogenic oocytes (0.75 mm in diameter) were incubated with 10 and 100 ng/ml NP or PCB126 with [^3H]17α-hydroxyprogesterone as a precursor. The major metabolites produced were androstenedione, testosterone (T), estrone, and estradiol-17b (E_2). Both NP and PCB126 increased T production and decreased E_2 production, except for 100 ng/ml PCB126. In experiment 2, oocytes (0.650.75 mm in diameter) were exposed to NP and PCB126 at different concentrations (0.01, 0.1, 1, 10, and 100 ng/mL). After the incubation, T and E_2 production was measured by radioimmunoassay. NP inhibited E_2 production at concentrations of 0.01 and 0.1 ng/ml in 0.75-mm-diameter oocytes. NP at 1 and 100 ng/mL stimulated T production, but had no observable effect on E_2 production. PCB126 treatment did not affect E_2 production at any of the concentrations tested. NP alone at 0.1 ng/mL resulted in a significant decrease in E_2 production in 0.65-mmdiameter oocytes. PCB126 did not show any significant effects on either T or E_2 production at all concentrations tested. These results suggest that NP acts like an antiestrogen at lower concentrations (0.01-0.1 ng/ml) in vitellogenic oocytes of redlip mullet.
In Vitro Steroidogenesis on Oocyte Development in the Starry Flounder, Platichthys stellatus
백혜자,김형배,Dea Geun Kim 한국발생생물학회 2013 발생과 생식 Vol.17 No.4
In this study, oocyte steroidogenesis are investigated in relation to oocyte development in the starry flounder, Platichthys stellatus, a marine multiple spawner. Vitellogenic (0.52 and 0.55 mm oocyte diameter) and mature oocytes (0.63, 0.66 and 0.71 mm oocyte diameter) were incubated in vitro in the presence of [3H]17α-hydroxyprogesterone ([3H]17α- OHP) as a precursor. Steroid metabolites were extracted from the incubated media and oocytes, the extracts were separated and identified by thin-layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography- mass spectrometry (GC-MS). The major metabolites produced from [3H]17α-OHP were androgens [androstenedione (A4) and testosterone (T)] and estrogens [17β-estradiol (E2) and estrone (E1)] and progestins [17α,20α-dihydroxy-4-pregnen- 3-one (17α20αP) and 17α,20β-dihydroxy-4-pregnen-3-one (17α20βP)] in vitellogenic and mature oocytes. The results from this study suggest the potential roles of E1 in the oocytes with diameter 0.52-0.71 mm, 17α20αP and 17α20βP at the oocytes of 0.63, 0.66 and 0.71 mm.
무지개송어 (Oncorhvnchus mvkiss) 난소여 포의 협막층 (Theca layers)을 통한 스테로이드 대사 분석
백혜자,이택열 한국통합생물학회 1993 동물학회지 Vol.36 No.1
난모세포성숙기 (peripheral germinal vesicle stage)의 무지개송어(Oncorhynchus mykiss)를 대상으로 방사성 표지 전구체(3H-pregnenolone, 3H-l7$\alpha$-hydroxyprogesterone 그리고 3H-androstenedione)를 이용하여 유 nitro에서 관찰한 협막세포층의 스테로이드 주 대사 경로는 다음과 같으며, pregnenolone -) 17a-hydroxypregnenolone$\longrightarrow$ 17u-hydroxyprogesterone $\longrightarrow$ androstenedione $\longrightarrow$ testosterone 이는 $\Delta$5-스테로이드 경로의 존재가능성을 지적하고 있다.
백혜자,김형배,노섬,이영돈,박무억 한국수산과학회 2004 Fisheries and Aquatic Sciences Vol.7 No.1
Plasma steroid hormone levels in the viviparous rockfish (Sebastes schlegeli) were examined in relation to gonadal histology under controlled photoperiods and water temperatures. To investigate those effects in S. schlegeli the photoperiod was maintained at 15L:9D in June and then it was gradually decreased to 9L:15D in October. It was then gradually increased to 12L:12D in January, followed by 14L:10D in February. The water temperature was 19-20℃ in July. From August to October, it was from 18℃ to 12℃. Then, it was dropped to a low of 9-11℃ in November to December and then gradually increased to 14-15℃ in February. In females, both plasma estradiol-17β (E2) and testosterone (T) levels from August to February showed a similar pattern in both the treatment and the control groups. In the treatment group, the peaks of plasma E2 and T were observed in November, and the peaks were closely correlated to histological observations. Oocytes contained many yolk globules (final vitellogenic oocytes), and oocytes at the migratory nucleus stage increased in size. Plasma levels of progesterone did not change much throughout the experimental period. However, in the control group, the peaks of E2, T, and progesterone were observed in February. These results indicate that the controlled photoperiod and water temperature accelerated sexual maturity, corresponding to the advancement of plasma E2 and T peaks by approximately 3 months. In males, plasma T levels showed a similar pattern from August to October in the treatment and control groups, though levels in the treatment group were higher than those in the control group. From histological observations, the treatment group copulated one month earlier.