낙동강 원수의 생물활성탄 및 오존처리공정에 따른 수질 변화 및 폴리오바이러스의 제거특성

정은영,박홍기,이유정,정종문,정미은,홍용기,장경립,Jung Eun-Young,Park Hong-Ki,Lee You-Jung,Jung Jong-moon,Jung Mi-Eun,Hong Yong-Ki,Jang Kyoung-Lib 한국생명과학회 2005 생명과학회지 Vol.15 No.5

오존처리는 음용수중의 유해한 미생물을 소독하는 처리기술로 주로 이용되고 있으며, 생물활성탄 처리기술 역시 오염물질 제거를 포함한 음용수 제조과정에 여러 가지 이점을 제공한다. 이글의 병행처리는 원수중의 여러 물질을 제거하는데 효과적인 공정으로 간주되고 있다. 본 연구에서는 낙동강 하류의 매리취수장 원수를 사용하여 생물활성탄 정수처리공정 및 오존에 의한 수질 변화와 함께 폴리오바이러스 제거 효율을 조사하였다. 수질인자들은 BAC 여과를 거치면서 $NH^{+}_{4}-N$등을 포함한 모든 항목들은 거의 제거되는 것으로 나타났다. Pilot-plant를 이용한 정수처리공정별 폴리오바이러스 제거실험에서는 전오존 접촉에 의해 $96.8\%$, 침전단계에서$99.3\%$, 여과단계에서 $99.6\%$의 바이러스가 제거되었으며, 후오존을 거친 BAC 여과수 시료에서는 세포배양법과 ICC-PCR 방법에서 바이러스가 $100\%$ 제거되어짐을 확인할 수 있었다. 오존농도에 의한 폴리오바이러스 제거실험 결과 0.4mg/1에서 5분간 접촉되었을 때는 약 $61.1\%$ 이상이, 0.8mg/1에서 10분 이상 접촉시킨 후에는 바이러스의 $100\% $가 불활성화 되어졌음을 알 수 있었다. Ozonation is a disinfection technique of harmful mi-crobes commonly used in the treatment of drinking water. And Biological Activated Carbon (BAC) treatment also provides numerous benefits for drinking water utilities, including removal of micro- pollutants, improved treatment processes. The multiful-stage ozonation and BAC play roles as effective methods for removing several materials in raw water. Water quality variation in Nak dong river and the removal efficiency of viruses by ozonation-BAC process were investigated on pilot scale. During the period of survey, most of water quality parameters including $NH_{4}^{+}-N$ were highly improved after passing through the BAC. The removal efficiency of poliovirus type III in water treatment process using pilot-plant,$ 99.6\% $ of viruses were removed by pre-ozonation, sedimentation and sand filteration process, $ 100\% $ were removed after in BAC filteration step. In the removal survey of viruses by ozonation, ap-proximately $ 61.1\% $ or polioviruses were inactivated by ozone of 0.4 mg/l within 5 min. and $ 100\% $ were inactivated by ozone of 0.8 mg/l over 10 min.

Helicobacter pyloir 양성 소화성 궤양에서 lansoprazole 포함 삼제요법의 제균율 및 제균판정에 있어서 요소호기검사의 유용성

정혜경,곽재진,유민아,배기선,권정미,이종수,김도영,문일환 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

목적 : 본 연구는 Helicobacter pylori (이하 H. polyri) 양성인 소화성 궤양 환자에서 lansoprazole 포함 삼제요법의 제균율을 알아보고, 제균 치료 후 제균 판정에 있어서 요소 호기 검사(Urea breath test, 이하 UBT) 및 신속요소분해(rapid urease test, 이하 RUT test)와의 일치율에 대하여 알아보고자 하였다. 방법 : 상부위장관내시경 검사에서 H. polyri 양성인 소화성 궤양환자에서 lansoprazole 60㎎+amoxicillin 2g+clarithromycin 1g의 약제를 2회 분복하여 1주간 복용하는 삼제요법을 실시하였다. 최소 4주후 추적 상부위장관내시경을 실시하였고, 전정부와 체부에서 각각 RUT를 실시하였으며 UBT(5분, 20분)를 시행하였다. 결과 : 대상환자는 총 46명으로 남자 34명(48±13세), 여자 12명(53±14세)이었다. LAC 삼제요법의 제균율은 40/46명(87.0%)이었고, 궤양의 치유율도 42/46명(91.3%)이었다. RUT와 20분 UBT 결과가 모두 음성이었던 경우는 41예, 모두 양성이었던 경우는 4예로 RUT와 UBT는 97.8% (45/46)의 일치율을 보였고, 1예는 UBT는 음성이었으나 체부에서 시행한 RUT가 양성이었다. 5분 UBT 검사가 양성이었던 예는 14/34 (41.2%) 이었고, 이 14예 중 12예(85.7%)는 20분 UBT 검사 음성, RUT 음성이었다. 결론 : Lansoprazole과 amoxicillin, clarithromycin 1주일 병합요법은 H. polyri 양성인 소화성 궤양 환자에서 87.0%의 제균율을 나타내어 우수한 효과를 보였고, 요소호기검사는 제균 치료 후 제균 판정에 유용한 비침습적인 방법임을 확인할 수 있었다. Background : There are only a few studies on Helicobacter pylori (H. pylori) for its eradication rates of lansoprazole-based triple therapy in Korea, and the results are controversial. Therefore, we undertook to investigate the eradication rate of lansoprazole-based triple therapy, and compare the concordance rate of urea breath test (UBT) and rapid urease test (RUT) in evaluating H. pylori eradication. Methods : Patients with acute peptic ulcer who were H. pylori-positive were recruited by prospective, consecutive manner. They received lansoprazole 30 ㎎ b.d., amoxicillin 1 g b.d. and clarithromycin 500 ㎎ b.d. for 1 week. Upper endoscopy was performed after 4 weeks to check for ulcer healing, and UBT and RUT were performed to evaluate H. pylori eradication status. Results : A total of 46 patients were recruited, and they were all compliant. H. pylori eradication rate was 87.0% (40/46) and ulcer healing rate was 91.3% (42/46). Forty one patients showed negative in both UBT and RUT, and 4 patients revealed positive in both tests, therefore, the concordance rate of UBT and RUT was 97.8% (45/46). Conclusion : Our study showed that 1-week lansoprazole-based triple therapy was effective in H. pylori eradication and ulcer healing. UBT can be an effective, noninvasive method for evaluating H. pylori status after H. pylori eradication.

RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석

정용환,한상현,오유성,고미희,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of GenusAbies distributed in Korea using random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) 2 sequences of the nuclear ribosomal DNA (rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation (base substitution) in 243 base pairs (bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species.

1-Arylpyrrole로부터 9-Arylcarbazole의 합성

朴유미,金寅植,李龍均,成大東,金東賢,金倫瑛,鄭大一 東亞大學校附設基礎科學硏究所 1997 基礎科學硏究論文集 Vol.14 No.1

Carbazoles were discovered in anthracene oil of coal tar, and are the parent structure of a number of heterocyclic compounds. Much attention has been paid to carbazole derivatives recently as the materials for semiconductors and photoconductive compounds. Many conventional methods for synthesis of carbazoles were known such as Grabe-Ullmann methods and the Tauber methods. In the course of the investigation for the synthesis of pyrrole derivatives 3. We have found the formation of 9-arylcarbazoles 4 under refluxing glacial acetic acid. Thus we report the results here.

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

인천 인현동 호프집 화재 피해자 분석

최정태,안무업,안희철,최영미,정재봉,서정열,유기철,이삼우,박석현,조준휘,김성환,김아진,송근정 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Purpose : This study was conducted to develop field triage, transportation, distribution, and prehospital care at a fire disaster by analyzing the victims of the fore that broke out at a bar in Incheon. Method : We analyzed the cases of the victims of a fire in Incheon in Oct. 1999. We determined the primary care hospital, the arrival time, the burn size, the outcome, and the injury type from the medical records, the concerned organ records, and interviews with concerned persons. Result : The total number of victims was 137: 56 prehospital deaths, 1 hospital death, and 80 survivals. The Pearson correlation coefficient between the burn size and the severity was -0.175. There were 121(89.6%) cases of inhalation injury, 59 (43.7%) cases of flame burns, 66 (48.9%) cases of hypoxic brain damage, and 16 (11.9%) cases involving other types of injury. Conclusion : The causes of death of the fire victims were inhalation injury and hypoxic brain damage due to CO poisoning and other toxic inhalants. We propose the use of a simple triage and rapid treatment(START) system and a reassessment the delayed category in fire disasters.

서울 居住主婦의 通過儀禮에 대한 意識과 儀禮飮食의 利用實態에 대한 硏究

손유미,정낙원,이효지 漢陽大學校 韓國生活科學硏究所 1992 韓國 生活 科學 硏究 Vol.- No.10

The purpose of this study is to investigate the actual situation of the transitional rites and to reestablish the meaning of that by grasping the relation among urban housewives' view, knowledge and behavior about transitional rites, by grasping the relation of those and background variables, by grasping the actual use -pattern of ritual food. The results are as follows 1.The view of the transitional rites shows significant difference according to educational level(p <0.001) and religion(p<0.001), and shows partial difference according to age, income, and residing shape. The older, the more traditional. With the less income, the more traditional. So do for the less educated, for Buddhist, and for those who live in a private residense. 2.The mean of knowledge about the transitional rites is 13.03. The knowledge shows significant difference according to religion, and shows partial difference according to educational level and income. the mean of knowledge is higher for the less educated, and for Buddhist. Those who earn more than 1500 thousand won have more knowledge than the other groups. 3.The behavior about traditional rites shows significant difference according to religion (p<0.001), residing shape(p<0.05), and shows partial difference according to age, educational level, family constitution, and whether employed or not. 4.There is close correlation(p<0.001) among the views, knowledge, and behavior. The behavior is traditional and the mean of knowledge is high as the view of the transitional rites is traditional.

벗나무속 수종간의 핵 리보솜 DNA의 ITS Ⅰ 염기서열 비교

오유성,김기옥,정용환,오문유,한상현,고미희 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

Comparison of four species (Prunus yedoensis, P. sargentii, P. pendula for. ascendens, P. serrulata var. quelpaertensis) and one cultivar of P. yedoensis were carried out by comparing their nucleotide sequences from the internal transcribed spacers (ITS) I of nuclear ribosomal DNA (rDNA). The length of the ITS I region was ranged from 229 to 236 base pairs (bp). The G+C contents of ITS I ranged from 58.5 to 60.2%. The value of nucleotide divergence across for four Prunus species and one cultivar ranged from 0.4 to 6%. The pairwise distance between P. pendula for. ascendens and P. yedoensis was 0.004 showing the lowest value among any other pairs. On the other hand, the pairwise distance betwen P. yedoensis and cultivated P. yedoensis was 0.026. The nucleotide analysis based on ITS I sequence was considerably similar to previous studies on the basis of random amplified polymorphic DNA (RAPD).

중환자실 환자에서 원내 칸디다혈증 발생의 위험 인자

이유현,최희정,이미애 대한감염학회 2004 감염과 화학요법 Vol.36 No.4

Background : Nosocomial candidemia is one of the most important disease entity in hospital not only because of increasing incidence but also because of its high fatality rate. The purpose of this study is to identify risk factors predicting the development of nosocomial candidemia and to evaluate its clinical outcome of nosocomial candidemia in the intensive care unit (ICU) patients. Materials & Methods : We conducted a retrospective, matched case-control study in adult patients admitted to ICU from January 1998 through December 2002 in Ewha Womans University Mokdong hospital. Sixty cases with candidemia were retrospectively identified from the records of the Department of Clinical Microbiology. The possible risk factors for the development of nosocomial candidemia were compared between 60 patients and 120 control subjects. Results : Candida albicans was the most commonly isolated organism. In the multivariate analysis, number of concomitant bacteremia (OR=17.3), number of candida infection in other site (OR=20.6), number of antibiotics used (OR=4.2), duration of indwelling central venous catheter (OR=16.1), and insertion of arterial line for more than 8 days (OR=6.9) were identified as significant risk factors for development of candidemia. Crude mortality rate was higher in candidemia patients compared with control subjects (P=0.004). Conclusion : The best predictors of development of candidemia in ICU patients were concomitant bacteremia, candida colonization in other site, combined use of antibiotics, and long-indwelling arterial and central venous catheter. For the ICU patients with high risk factors of candidemia, we could consider the intervention to prevent emergence of candidemia. 목적 : 원내 감염 칸디다혈증은 최근 의학 기술의 발전과 함께 면역 기능이 감소된 경우 특히 발생 빈도가 증가할 뿐 아니라 높은 치사율로 그 중요성이 강조되고 있다. 이 연구의 목적은 중환자실에 입원한 환자에서 칸디다혈증 발생의 위험 요인과 이로 인한 사망률을 파악하는데에 있다. 재료 및 방법 : 1998년 1월부터 2002년 12월까지 5년간 이화여대 목동병원 중환자실에 입원하였던 환자를 대상으로 후향적으로 입원 기록을 검토하여 환자-대조군 연구를 시행하였다. 환자군은 진단 검사의학과 미생물실 자료에 의거, 칸디다혈증이 있었던 60명을 대상으로 하였고 대조군은 같은 시기에 중환자실에 입원하였으나 칸디다 혈증은 없었던 120명으로 짝지어 각기 28개의 가능성있는 위험 인자와 치사율에 대해 조사하였다. 결과 : 칸디다혈증을 유발하는 균종은 Candida albicans가 24예(40%)로 가장 흔하였다. 다변량 분석을 통해서 균혈증이 동반된 경우(OR=17.3), 칸디다가 다른 부위에서도 검출된 경우(OR=20.6), 항생제 병용이 많을수록(OR=4.2), 중심정맥관 삽입 기간이 길수록(OR=16.1), 동맥관 삽입이 8일 이상인 경우(OR=6.9)가 중환자실 환자에서 칸디다혈증 발생의 독립적인 위험인자이었다. 보통 사망률(Crude mortality)은 환자군에서 48.3%, 대조군에서 26.7%로 칸디다혈증 환자에서 사망률이 유의하게 높았다(P=0.004). 결론 : 칸디다혈증의 발생은 균혈증이 동반된 경우, 다른 부위에서 칸디다가 검출된 경우에 증가하며, 항생제 병용이 많을수록, 혈관 내에 중심정맥관이나 동맥관이 장기간 유치되어 있을수록 증가하였다. 칸디다혈증의 사망률 역시 높아, 칸디다혈증의 위험이 높은 중환자실 환자를 조기에 색출하고 칸디다혈증 발생을 예방할 수 있는 방법들에 대한 연구가 필요하겠다.

Siloxypyridinium Salt와 Silyl Enol Ether와의 반응에 대한 연구

이용균,정대일,최순규,박유미 東亞大學校 大學院 1998 大學院論文集 Vol.23 No.-

N-Ethoxycarbonylsiloxypyridinium chloride(3) was generated in solution(acetonitrile) from siloxypyridine(2) and ethylchlorofomate and reacted to silyl enol ether(4a, 4b) at 4-position exclusiely to afford 4-substituted pyridone derivatives(5a, 5b) in moderated yields.