자발성 단독 상장간막동맥 박리의 임상적 특성

고미희,오용해,김갑득 대한응급의학회 2013 大韓應急醫學會誌 Vol.24 No.6

Purpose: Spontaneous isolated superior mesenteric artery dissection (SISMAD) is defined as superior mesenteric artery (SMA) dissection without an associated aortic dissection. SISMAD is an uncommon mesenteric ischemia, but can lead to death if not recognized early. Here, we present our experience on features characterizing SISMAD in an early period and review imaging findings. Methods: Twelve symptomatic SISMAD patients who received conservative treatment between March 2005 and February 2012 were included in this study. Their clinical characteristics,imaging findings, treatment methods and outcomes were retrospectively analyzed. A diagnosis of SISMAD was confirmed by multidetection computed tomography (MDCT) and computed tomographic (CT) angiography. Results: All patients complained of severe (6 patients) or moderate abdominal pain (6 patients), and 4 patients complained of radiating back pain at the same time. Eight patients had an acute onset (≤3 days) of abdominal pain,and 4 patients had a chronic onset of the abdominal pain. The abdominal pain was located in the upper abdomen in 7patients and in the periumbilical area in 5 patients. Angiographic types of SISMAD were classified into Sakamoto’s type I (3 patients), type II (1), type III (4), and type IV (4). The dissection occurred within 3 cm from the orifice of the SMA in 9 patients (75%). The angiographic type of SISMAD was not associated with clinical symptoms and treatment methods. Ten out of 12 patients were treated with conservative management such as expectation and anticoagulation. Aneurysmal dilation of the SMA was noted in 4 patients, and 2 patients out of these 4 underwent surgical operations due to persistent abdominal pain and a growing aneurysm. No patients had a recurrence of symptoms and lesion progression on follow-up CT angiography. Conclusion: Patients with an acute onset of severe abdominal pain or chronic persistent abdominal pain should be suspicious of SISMAD and CT angiography should be performed.

우리교회 명교사를 소개합니다 : 강성경 교사

고미희 장로회신학대학교 기독교교육연구원 2007 교육교회 Vol.359 No.-

수학과 특별보충과정 이수학생들의 행동특성 변화에 관한 연구

고미희,이덕호 한국학교수학회 2004 韓國學校數學會論文集 Vol.7 No.1

본 논문에서는 제7차 교육과정의 단계형 수준별 교육과정에서 특별보충과정 이수학생들의 행동특성 변화를 분석하였다. 중학교 1학년 7-가 단계 특별보충과정 이수자를 대상으로 특별보충과정에 대한 반응을 조사하고 자아개념, 성취동기, 학습습관, 학습태도, 수학교과에 대한 흥미 등을 일반학생들의 행동특성과 비교 분석하였다. 분석결과 특별보충과정이 이수학생들의 행동특성 변화에 긍정적인 영향을 줄 수 있음을 알 수 있었다.

벗나무속 수종간의 핵 리보솜 DNA의 ITS Ⅰ 염기서열 비교

오유성,김기옥,정용환,오문유,한상현,고미희 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

Comparison of four species (Prunus yedoensis, P. sargentii, P. pendula for. ascendens, P. serrulata var. quelpaertensis) and one cultivar of P. yedoensis were carried out by comparing their nucleotide sequences from the internal transcribed spacers (ITS) I of nuclear ribosomal DNA (rDNA). The length of the ITS I region was ranged from 229 to 236 base pairs (bp). The G+C contents of ITS I ranged from 58.5 to 60.2%. The value of nucleotide divergence across for four Prunus species and one cultivar ranged from 0.4 to 6%. The pairwise distance between P. pendula for. ascendens and P. yedoensis was 0.004 showing the lowest value among any other pairs. On the other hand, the pairwise distance betwen P. yedoensis and cultivated P. yedoensis was 0.026. The nucleotide analysis based on ITS I sequence was considerably similar to previous studies on the basis of random amplified polymorphic DNA (RAPD).

제주도 재래종말에서 혈액 단백질의 유전적 변이에 관한 연구

김세재,정창조,오문유,김규일,김기옥,고미희 濟州大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.5 No.1

In order to clarify the genetic composition and to find possible genetic markers of Cheju native Horses, the genetic variations of the nine proteins (Al, Pi, Es, Hp, Hb, PGD, PGM, MDH, ME) were investigated by the gel electrophoretic techniques. Al: there were two alleles, AlA and AlB. The alleles frequencies of AlA and AlB were 0.398 and 0.602, respectively(X²=0.02, d.f.=2, P>0.9). Pi: Seven Pi alleles(PiF, PiG, PiI, PiL, PiN, PiS and PiU) were detected. The gene frequencies of Pi were PiG 0.136, PiL 0.496, PiN 0.260, and other(PiF, PiI, PiU, PiS) 0.108, respectively(X²= 62.48, d.f.=12, P<0.005). Es: There were three alleles, EsF, EsI and EsS. The frequencies of Es were EsF 0.266, EsI 0.712, and EsS 0.022, respectively(X²= 21.28, d.f.=3, P<0.005). Hb: Three alleles (HbA, HbBI, and HbBII) were found. The gene frequencies of Hb were HbA 0.120, HbBI 0.620 and HbBII 0.260, respectively(X²=2.63, d.f.=3, P>0.25). PGM: There were two alleles, PgmF and PgmS The gene frequencies of PGM were PgmF 0.434 and PgmS 0.566, respectively(X²=0.22, d.f.=2, P>0.75). PGD: There were two alleles, PgmF and PgmS. The gene frequencies of PGD were PgmF 0.823 and PgmS 0.177 respectively(X²=7.53, d.f.=2, P<0.05). Three proteins (Hp, MDH, ME) showed the monomorphic traits in this population. The mean heterozygosity of nine protein loci were calculated as 0.329±0.012.

장애유형별 구강건강실태에 관한 조사

고미희 ( Mi Hee Ko ) 한국치위생과학회 2004 치위생과학회지 Vol.4 No.1

흰쥐 뇌에서 속후각겉질 손상 후 해마로 투사하는 콜린성 신경로의 변화에 관한 연구

고미희(Mi-Hee Ko),김대성(Dae-Sung Kim),변경희(Kyung-Hee Byun),김재우(Jae-Woo Kim),김명주(Myeong-Ju Kim),오문유(Moon-You Oh),이봉희(Bong-Hee Lee) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.1

흰쥐 뇌에서 해마로 투사하는 신경로와 콜린성 신경로를 구축한 뒤 속후각겉질 손상 후에 해마로 투사되는 두 신경로 의 형태학적인 변화를 조사하기 위하여 pseudorabies 바이러스의 Bartha stain을 신경로 추적자로 이용하여 다음과 같은 결과를 얻었다. 안쪽격벽핵 (medial septal nucleus), 줄무늬침상핵 (bed nucleus stria terminalis) 등 10여 핵에서 양성반응이 나타나 해마로 투사하는 신경로를 구축하였으며 무과립 섬겉질 (agranular insular cortex), 중심편도핵 (central amygdala)등의 여러 신경핵들이 해마로 투사하는 콜린성 신경로를 구성하는 신경핵으로 조사되었다. 이 중 신경핵의 종류에 따라 이중양성반응 정도에는 차이를 보여 콜린성 신경로는 신경핵에 따른 차이를 보였다. 속후각겉질 손상 후 10일과 30일에 해마로 투사하는 신경로와 콜린성 신경로의 형태학적 변화를 조사한 결과 내측안쪽무릎핵 (medial geniculate complex, medial part)과 내측배쪽무릎핵 (medial geniculate complex, ventral part) 및 시상(thalamus)등 일부 신경핵에서 양성반응을 보이는 신경세포들의 수가 현저히 감소하였다. 이상의 결과를 종합해 볼 때 Alzheimer’s 질환 모델인 속후각겉질 손상 흰쥐에서 신경로 구성 세포, 콜린성 신경세포 및 신경핵들이 감소되어 치매시의 신경손상이 신경로를 따라 일어 나며 신경세포 수의 감소와 신경전달물질의 감소가 동반 될 것으로 사료된다. The hippocampus is known as involved in learning and memory functions and the entorhinal cortex plays a crucial role as a gateway connecting the several areas and hippocampal formation. Entorhinal cortex lesions have been employed in numerous studies as the Alzheimer’s disease model. The purpose of this study were to identify the CNS hippocampal and cholinergic pathway and to investigate the morphological changes of the hippocampal cholinergic innervations by using the Pseudorabies virus injection into the hippocampus after entorhinal cortex lesions. The pseudorabies virus and double labelled neurons (ChAT and PRV) were distributed at several different nuclei including agranular insular cortex, bed nucleus of stria terminalis, central amygdala, globus pallidus, lateral segment, lateral hypothalamic area, laterodorsal tegmental nucleus, medial septal nucleus, mesencephalic reticular nucleus, periaqueductal gray matter and substantia innominata The morphological changes were observed in the hippocampal cholinergic innervation after entorhinal cortex lesions. These data suggested that the hippocampal cholinergic innervation showed morphological changes throughout the whole brain areas after entorhinal cortex lesion.

한라산 중산간 목장의 소똥구리과(Scarabaeidae) 분포

양경식,강성혁,민동원,김동순,고미희,정용환 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

한라산 중산간 지역은 최근 인위적 간섭으로 인해 소똥구리과(Scarabaeidae)의 서식지가 소멸되는 있어, 소똥구리과를 구성하는 종들의 상대수도, 종풍부도, 종다 양도를 파악하여 소똥구리과 분포에 관한 기초자료를 제공하고자 실시하였다. 본 조사는 2013년 5월부터 9월까지 한라산 제2 산록도로(1115지방도)변 중산간 목장 지역 6곳(남원읍 신례리, 서귀포시 동홍동, 대천동, 하원동, 색달동, 한림읍 금악리) 을 대상으로 월 3회 실시하였으며, 각 지역마다 함정덫(Pit-fall Trap)을 5m 간격으 로 6개씩 설치하여 닭내장으로 유인하였다. 조사결과 렌지소똥풍뎅이(Onthophagus lenzii) 806개체, 제주도소똥풍뎅이(O. ohbayashii) 455개체, 모가슴소똥풍뎅이 (O. fodiens) 246개체, 멸종위기Ⅱ급 보호종인 애기뿔소똥구리(Copris tripartitus) 가 94개체 및 혹가슴검정소똥풍뎅이(O. atripennis) 12개체 등 총 1,613개체가 조 사되었다. 종풍부도(Richness Index, RI)에서 다수종(Abundant)은 렌지소똥풍뎅 이와 제주도소똥풍뎅이였고, 애기뿔소똥구리 등 3종은 희소종(Rare)으로 나타내 었다. 각 지역별 종다양도(H’)는 하원동(1.25), 대천동(1.22), 색달동(1.22), 동홍동 (0.96), 금악리(0.51), 신례리(0.45) 순이며, 월별 종다양도는 8월(1.29), 5월(1.14), 6월(1.09), 9월(1.05), 7월(0.80)순으로 나타내었다. 특히, 본 조사 결과 멸종위기Ⅱ 급 보호종 애기뿔소똥구리는 금악리를 제외한 중산간 목장 지역 전체가 서식지로 파악되어 한라산 중산간 목장 지역에 대한 체계적인 보전대책 마련이 시급하다고 사료된다.

제주재래미와 쓰시마재래마에서 미토콘드리아 DNA 의 제한효소 분석과 진화학적 유연관계

오문유,고미희,김기옥,오유성,정용환,김세재,홍성수,모야박 한국축산학회 1997 한국축산학회지 Vol.39 No.4

Mitochondrial DNA(mtDNA) restriction endonuclease fragment patterns were studied in four different equine populations(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds). Total DNA samples from 122 individuals were analyzed with 18 restriction enzymes, Bam HI, EcoRI, HindIII, AvaII, HpaI, BgIII, XbaI, PstI, Pvu II, Sac I, Kpn I, Xho I, AvaI, Hinc II, Bst EII, Not I, Acc I, and Hae II using the horse mtDNA probe. Twelve morphs were observed yielding sixteen different combinations of fragment patterns(mtDNA types) in four populations. The phylogenetic tree constructed from the 12 restriction types by the UPGMA method indicated that mtDNA types in four populations were divided into two clusters. In this tree, the clear separation into the two clusters was observed, namely one with types 1, 6, 3, 8, 9, 2, and 5, and the other one with types 4, 7, 10, 11, and 12. Tsushima native horses were found to be highly homogeneous by the frequencies of mtDNA types. The populations of Cheju native horses I, II, and Thoroughbreds showed polymorphism, while the population of Tsushima native horses was monomorpic(only 1-2-1-1 type), according to the restriction enzymes. Cheju native horses II were shown to be closely related to the Tsushima native horses, and the genetic distance between these two populations was 1.5×10^(-3).

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.