[특집 : 국제화시대의 제주도연구(자연과학분야)] 제주도 인류집단의 혈액 및 타액단백질의 다형현상과 유전적 변이

오문유 제주학회 1988 濟州島硏究 Vol.5 No.-

단방조충(單房條蟲)의 형태(形態)와 생활사(生活史)에 관(關)한 연구(硏究)

오문유,김오남,한방근,김영홍,Oh, Moon You,Kim, O Nam,Han, Bang Keun,Kim, Young Hong 대한수의학회 1976 大韓獸醫學會誌 Vol.16 No.2

The purposes of this study were to define the life cycle of Echinococcus and its species and subspecies in Korea. The results were as followings: 1. The patent period day of Echinococcus granulosus was 50 days. 2. The life cycle of Echinococcus granulosus was dog-cattle-dog, as cattle-dog originating adult. 3. The sine of the adult worm was measured from 2.2mm to 6.2mm, but most of them was between 3.0mm and 4.0mm (40% of all). 4. Although the number of segments was from three to five proglottids, most of them was 3 proglottids(76.7%), as immature, mature and gravid, respectively. 5. The habitat of the adult worm was recognized as duodenum or pyloric region in the dog. 6. According to the results above, we could be proved that the hydatid cysts of he Echinococcus discovered in Jeju-do, was Echinococcus granulosus granulosus.

제주재래미와 쓰시마재래마에서 미토콘드리아 DNA 의 제한효소 분석과 진화학적 유연관계

오문유,고미희,김기옥,오유성,정용환,김세재,홍성수,모야박 한국축산학회 1997 한국축산학회지 Vol.39 No.4

Mitochondrial DNA(mtDNA) restriction endonuclease fragment patterns were studied in four different equine populations(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds). Total DNA samples from 122 individuals were analyzed with 18 restriction enzymes, Bam HI, EcoRI, HindIII, AvaII, HpaI, BgIII, XbaI, PstI, Pvu II, Sac I, Kpn I, Xho I, AvaI, Hinc II, Bst EII, Not I, Acc I, and Hae II using the horse mtDNA probe. Twelve morphs were observed yielding sixteen different combinations of fragment patterns(mtDNA types) in four populations. The phylogenetic tree constructed from the 12 restriction types by the UPGMA method indicated that mtDNA types in four populations were divided into two clusters. In this tree, the clear separation into the two clusters was observed, namely one with types 1, 6, 3, 8, 9, 2, and 5, and the other one with types 4, 7, 10, 11, and 12. Tsushima native horses were found to be highly homogeneous by the frequencies of mtDNA types. The populations of Cheju native horses I, II, and Thoroughbreds showed polymorphism, while the population of Tsushima native horses was monomorpic(only 1-2-1-1 type), according to the restriction enzymes. Cheju native horses II were shown to be closely related to the Tsushima native horses, and the genetic distance between these two populations was 1.5×10^(-3).

小黑山島 住民의 PTC (Phenylthiocarbamide) 味覺역値 및 色感異常者의 頻島에 관한 硏究

吳文儒 제주대학교 1982 논문집 Vol.14 No.-

小黑山島內 국민학생 262名을 對象으로 PTC 味覺역値 및 色感異常者의 頻度를 調査하여 다음과 같은 結論을 얻었다. 1. 男子 132名中 PTC味覺역値는 溶液 No.9에서 40名(30.3%)으로 가장 높았으며, 女子는 130名中 No.10에서 37名(28.46%)로 가장 높았으며, 全體的으로는 No.9에서 76명(29.01%)으로 가장 높은 頻度를 나타내었다. 다시 말해서 Mode는 No.9에서 나타났으며 Antimode는 No.3에서 나타났다. 2. 262名中 taster는 253名으로 전체의 96.56%, non-tasters는 9名으로 3.44%이었다. Non-taster의 頻度는 다른 섬지방인 大黑山島와 紅島의 11.8%, 內陵地方의 15.1%와 比較하여 매우 낮은 頻度를 나타내었다. 3. PTC 平均역値는 男子 8.52, 女子 8.76으로 서로 類似하였으며 (total 8.64), 他集團과 비교하면 female에서 內陸地方보다 낮은 便이었다. 4. Ishihara plates를 使用하여 色感異常有無를 조사한 結果 262名中 단 1名의 色感異常者도 發見치 못하였다. Against 132 of male and 130 of female on Sohuksan-do isolated from main land, the threshold tastability of PTC and the frequency of color defects were studied. The results are as follows: 1. They showed bimodal distribution in tastability with the mode at number 9 and antimode at number 3. The frequency of non-tasters in this population was the lowest (3.44%) among those of other islands(11.8%) and inland populations(15.1%). 2. The frequency of non-tasters of male(4.55%) to female(2.31%) was not different significantly. 3. The average taste threshold the PTC between two sexes was not different significantly, 8.52±0.43 in male and 8.76±0.45 in female; the total average taste threshold was 8.64±0.44. 4. The color defect was not showed even one among 232 students. 5. It is assumed that the lowest frequency of non-tasters to PTC and of color defects was resulted from genetic isolation among the populations of other islands and mainland.

濟州島人의 遺傳學的 硏究Ⅱ : 色感異商者의 頻度에 대하여 On the Frequency of Color Defects

吳文儒 제주대학교 1979 논문집 Vol.11 No.-

I studied on the frequency of color defects in children of some primary schools with Ishihara plates, as one of the follow-up studies on the Jejudonian population genetics. The results are as follows: 1. The frequency of color defects in male was 3.417, and female 0.116. The color defects in Jeju-do showed lower than that in Seoul. 2. The color defects being classified into two groups, protanoids and deuetranoids, the frequency of male protanoids was 0.788, and that of deuteranoids was 2.628%. The frequency of female protanoids was nothing, and 0.116% was the frequency of frmale deuteranoids. The deuteranoids showed higher than the protanoids in both male and female. 3. The frequency of color defects in Jeju-do was the lowest among Seoul and Japan.

濟州島人이 遺傳學的 硏究 I : 産院分娩兒의 出生性比에 대해서 On the Secondary Sex Radio of the Newborns from Maternity Hospitals

吳文儒 제주대학교 1978 논문집 Vol.10 No.-

The data obtained from the five maternity hospitals in Jejudo. There are about 7,024 deli- veries from 1971 to 1978 which were analysed biologically to find the genetic peculiarity of Jejudo- nian populations. The sex ratio at birth was very high compared to other populations on the world as Russell(1987) had measured. The ratio was 113.17. The sex ratio at birth was 108.86 on 1971, 116.29 in 1972, 117.95 in 1973, 124.49 in 1974, 110.53 in 1975, 117.34 in 1976, 106.12 in 1977, 111.89 in 1978. The ratio is higher when the mothers are younger but as the mothers ages are increassing, the ratio is decreasing inversely. The sex ratio is not correlated with delivery time, as the sex ratio is 111.76 during day, and 115.21 at night. The sex ratio at birth by the pregnant season, is highest in spring but the lowest in fall, showing that 118.01 in spr- ing, 111.21 in summer, 108.90 in autumn, and 114.10 in winter.

韓國のオンブバッタおよびショウリョウバッタモドキ 集團における 染色體變異

茅野愛子,茅野博,吳文儒 濟州大學校 基礎科學硏究所 1993 基礎科學硏究 Vol.6 No.1

Chromosomes of primary spermatocytes of 17 males of Atractomorpha bedeli collected in a population at Haenam, Chollanam Do. Republic of Korea, were observed. Supernumerary chromosomes (B-chromosomes) were found in five males (2n??=18+X+0~3B, figs, 1-6). The numbers of B's were constant among the cells within the follicles but were variable among the follicles within the testes of the males (Table 1). The characteristics of the B's were the same as those found in the populations in Kyushu, Japan (Sannomiya 1973). The 9th chromosomes (the smallest among the complement) of A. bedeli were of two types, S with a distal supernumerary segment, i.e. segmented, and N, non-segmented (Figs, 1-6, 7-10, 11-16). The supernumerary segment was euchromatic (Figs, 11 and 12). Three kinds of karyomorphs were distinguishable as follows: S/S (a single male), S/N (five males), and N/N (1] males). Chromosomes of primary spermatocytes of 14 males of Gonista bicolor collected in a population in Chjudo, Cheju Do, Republic of Korea, were observed. Seven males were found to have B-chromosomes of two type, B₁ and B₂ (2n??=22+X+0~1B₁+0~4B₂; Figs. 17~22). The B₁'s were found in two males in which all the primary spermatocytes had a single B₁. The B₂'s were found in five males. The numbers of B₂'s were variable among the follicles of the testes, while the primary spermatocytes within the follicles had the same number of B₂'s (Table 3). The characteristics of B₁and B₂were the same as those found in the populations in Kyushu, Japan (Sannomiya 1974). 1.韓國全羅南道海南において授集したオンブバッタの雄17個體の第一精母細胞の染色體を觀察した. 12個體では9個の2價染色體と1個の1價染色體(X染色體)が見られたが, 5個體では, 前記の染色體の外にB染色體をもっていた(2n?=18+X+0~3B). B染色體の數は同一の個體の精巢でも, フォリクルによって異なっていた. しかし. 1つのフォリクルの中では, どの第一精母細胞にも同じ數のB染色體が見られた. 5個體のそれぞれにおけるB染色體の數の變動の範圍は, 0~2, 0~2, 1~2, 0~3, および, 0~3であった. これらのB染色體の細胞學的特徵は, Sannomiya(1973)が日本(九州)の集團について報告したものと同じであった. 2.韓國海南のオンブバッタ集團では, 常染色體の第9染色體に, 過剩部分をもつS型と, 過剩部分をもたないN型とが區別された. この過剩部分は眞正染色質であった. 二價染色體の特徵から, 接合體の染色體型として, S/S(1個體), S/N(5個體), および, N/N(11個體)の3型が識別された. 3.韓國濟州島のショウリョウバッタモドキ集團から雄14個體を採集し, 第一精母細胞の染色體を견察した. 7個體では11個の二價染色體と1個の一價染色體(X染色體)をもっていたが, 他の7個體には前記の染色體の外に, B染色體が含まれていた(2n=22+X+0~1 B₁+0~4 B₂). B₁は2個體に, また, B₂は5個體に見られた. B₁はどの第一精母細胞にも1個見られた. しかし, B₂の數は同一の個體の精巢の中でも, フォリクルによって異なっていた. 5個體のそれぞれにおけるB₂染色體の數の變動の幅は, 1~2, 1~2, 1~2, 1~3, および, 2~4であった. B₁およびB₂の特徵は, 日本(九州)のショウリョウバッタモドキの集團について, Sannomiya(1974)が報告したB染色體と同じであった.

제주도 畜犬의 犬사狀충에 관한 연구

金永洪,吳文儒 제주대학교 1973 논문집 Vol.5 No.-

1. Examining the blood of 440 dogs in Jeju Do by using the Modified Knott Method from April to December in 1973, 49 dogs were infected 11.1% positive. (In the blood examination in July, 19 dogs were infected among 90 dogs 21.1% positive) Measuring the microfilariae found in the blood examination, the length averaged 304.1μ(292-321μ) and the width 6.89μ(6.7-7.1μ). According to the result, the author could find all the microfilariae found in the blood examination of the dogs in Jeju Do, were only Dirofilaria immitis. 2. According to this study, microfilariae appeared in the priphery of the dog from April, increased rapidly in July, decreased gradually in October, and disappeared nearly in December from the periphery. 3. The maximal density of microfilariae in the periphery was happened at midnight, but the minimal density at midday. 4. Though mosquitoes (Ae togoi; 105, C.p.p.; 63, Ae. alb.;12) were collected from the vicinity of the infected dogs' houses examined under the stereoscope, no microfilariae were found.

단백질 전기영동상에 의한 제주도 사철란속 4종간의 유전적 유연관계

오문유,송성희,김세재 제주대학교 방사능이용연구소 1989 연구보고 Vol.4 No.-

제주도 사철란속(Goodyera) 4종(Goodyera velutina, G. maximowicziana, G. schlechtendaliana, G. macrantha)에 대하여 SDS polyacrylamide gel electrophoresis (SDS PAGE)와 two-dimensional gel electrophoresis(2-DGE) 방법을 이용하여 유전적 유연관계를 조사 하였다. SDS PAGE 방법을 이용한 사철란속 4종간의 단백질 pattern은 서로 유사 하였다. 2-DGE 방법을 이용한 genetic distance는 G. maximowicziana와 G. schlechtendaliana가 0.5773으로서 가장 가까운 유연관계를 나타내었고, G. velutina와 G. macrantha간의 genetic distance는 0.5858로 나타났다. 이들에 비해 G. maximowiczina와 G. macrantha간의 genetic distance는 0.7378로서 가장 먼 유연 관계를 나타내었다. 이상과 같은 결과로 미루어 볼 때 사철란속 4종간의 유전적 유연관계는 이들의 생태적 특징과 거의 일치하는 것으로 사료된다. The genetic relationships among four species(Goodyera velutina, G. marimowicziana, G. shlechtendaliana, G. macrantha) in Cheju island were examined by one dimesional(1-D) and two-dimensional gel electrophoretic(2-D) methods. The protein patterns of four species analysed by 1-D were relatively similar to one another. The genetic distances among four species were estimated by analysing the protein spots obtained by 2-D. The genetic distances between G. maximorowicziana and G. shlechtendalina, G. velutina and G. macrantha, G. maximowicziana and G. macrantha 0.5773, 0.5858, 0.7378, respectively. The genetic relationships among four species estimated in this study were coincident with their ecological characteristics.

濟州道 雜犬의 繁殖生理에 關한 硏究 : 發情 持續期間에 對하여

吳文儒,金永洪,玄寬宗 제주대학교 1976 논문집 Vol.8 No.-

An observation was made on the duration of estrus and the blood values during the estrous cycles in 15 mongrel bitches. The results obtianed were as follows ; 1. The length of proestrus was reckoned up the time from first appearance of blood in the vaginal smears to first acceptance of the male; varied 4 to 12 days (mean±S.D.=7.8±2.6 days). 2. The duration of estrus was determined by the acceptance test following the days between first and last acceptance; varied from 4 to 13 days (8.1±2.6 days). 3. The duration of visible bieeding out of vigina varied from 12 to 23 days (16.9±2.6days). 4. The seasonal occurrence of estrus did not exhibit. 5. During proestrus and estrus, the erythrocyte numbers increased 5-6% above those of established for beagle in anestrus. No significant variation in total leucocyte numbers was noted during the estrous cycles.