Perspectives of Integrative Cancer Genomics in Next Generation Sequencing Era

Kwon, So-Mee,Cho, Hyun-Woo,Choi, Ji-Hye,Jee, Byul-A,Jo, Yun-A,Woo, Hyun-Goo Korea Genome Organization 2012 Genomics & informatics Vol.10 No.2

The explosive development of genomics technologies including microarrays and next generation sequencing (NGS) has provided comprehensive maps of cancer genomes, including the expression of mRNAs and microRNAs, DNA copy numbers, sequence variations, and epigenetic changes. These genome-wide profiles of the genetic aberrations could reveal the candidates for diagnostic and/or prognostic biomarkers as well as mechanistic insights into tumor development and progression. Recent efforts to establish the huge cancer genome compendium and integrative omics analyses, so-called "integromics", have extended our understanding on the cancer genome, showing its daunting complexity and heterogeneity. However, the challenges of the structured integration, sharing, and interpretation of the big omics data still remain to be resolved. Here, we review several issues raised in cancer omics data analysis, including NGS, focusing particularly on the study design and analysis strategies. This might be helpful to understand the current trends and strategies of the rapidly evolving cancer genomics research.

OB-63 : Positive effects of Teratogenic risk counseling in pregnant women inadvertently exposed to medications

( Hyo Yeon Gwak ),( Jung Eun Lee ),( Ju Young Choi ),( So Yeon Kim ),( Song Mi Park ),( June Seek Choi1,),( Hyun Kyong Ahn ),( Min Hyung Kim ),( Jin Hoon Chung ),( Moon Young Kim ),( Hyun Mee Ryu ),( 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: To evaluate the positive effects of teratogenic risk counseling in pregnant women inadvertently exposed to medication in early pregnancy. 방법: In a prospective cohort study, 250 pregnant women exposed to medications in early pregnancy were recruited after teratogenic risk counseling at Korean MotherSafe Counsling Center, which has given information about teratogenic risk of medication to pregnant women. Positive effects are evaluated by visual analogue scale(VAS) for perception on teratogenic risk and percent prefering termination of pregnancy after medication exposures in pregnancy. 결과: Mean age of participants is 31.8±5.3years old. Mean gravidity is 2.4±2.1. Medications exposed in early pregnancy include antibiotics, analgesics, anti-inflammatory drugs, antacid and so on. Mean perceptive teratogen risk before and after the counseling is 33.9±1.6% and 15.8±1.6%, respectively. And mean percent prefering termination of pregnancy (≥5) before and after the counseling is 31.1±1.9% and 13.5±1.7%, respectively. 결론: Our data suggest that information on teratogenic risk for pregnant women inadvertently exposed to medication may have positive effects for reassuring pregnant women and preventing termination of pregnancy.

여성 정신분열병 환자에서 월경전기 증상변화의 양상

최소현,감상범,조숙행 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

연구목적 : 여성 정신분열병 환자에서 월경전기의 증상변화 여부와 그 변화양상을 알아보고자 본 연구를시행하였다. 방법 : DSM-lV 진단기준에 부합되며 규칙적인 월경주기와 월경기간을 보인 여성 정신분열병 환자 30명을 대상으로 한 번의 월경주기 동안 연구가 시행되었다. 매일평가서(Daily Rating Form)는 대상자들이 매일 작성 하였고 BPRS는 각 월경기(월경전기, 월경기, 월경후기)에 한차례씩 평가되었다. 연구기간동안 매일평가서를 올바르고 완전하게 작성한 24명에 대한 자료분석이 시행되었다. 결과 : 전체대상군의 총BPRS 평균정수는 세 월경기 간에 유의한 차이가 있었는데, 월경전기가 가장 높고 월경후기가 가장 낮았다. 그러나 증상군별로 볼 때 정신병적 증상군에 대한 전체대상군의 BPRS 평균점수가 각 월경기 사이에 유의한 차이가 없는 반면 불안-우울군과 위축-지연군에 대해서는 각 월경기 간에 유의한 차이가 있었다. 매일평가서 상에서 30% 변화기준을 적용하여 월경전기에 악화되었다고 판정되는 항목들 중 우울한 기분, 불안초조와 예민, 적개심과 공격성, 활동성지연등의 정서와 행동증상 항목이 높은 빈도를 보였다. 또한 복통, 유방통, 두통의 신체증상 항목도 월경전기에 악화되는 소견을 보였다. 결국 월경전기에 BPRS 상에서 정신병적 증상의 변화는 거의 나타나지 않는 반면 매일평가서와 BPRS 모두에서 이 시기에 정서 및 행동증상의 변화는 두드러졌다. 결론 : 본 연구의 소견들은 여성 정신분열병 환자에서 월경전기 증상의 악화가 정신병적 증상 그 자체의악화라기 보다는 정서적, 행동적, 신체적 증상들을 특징으로 하는 특정 증상군이 이 시기에 중첩되는 것에 기인할 수도 있음을 시사해 준다.

강원대학교 구내림 이용행태에 대한 설문분석

최정기,구소영,조선아,원종현 江原大學校 森林科學硏究所 2003 Journal of Forest Science Vol.19 No.-

본 연구는 강원대학교 학교 숲 이용자들의 행태를 분석하기 위하여 학생, 교수, 행정직원, 외부 방문자를 대상으로 설문조사를 실시하였다. 학교 구내림 필요성에 대한 질문에 대해서 학생이용자는 97%, 교수 이용자는 100%, 행정직원 91%가 필요하다고 응답하였고, 학교구성원의 80%이상이 구내림을 이용하는 것으로 나타났다. 가장 많이 이용하는 구내림 장소로는 학생과 행정직원은 연적지, 교수는 예술대 뒷산으로 조사되었으며, 구내림 이용동기에 대한 질문에는 학교구성원 모두 산책을 위해서라는 답변이 가장 많았다. 구내림 만족도에 대한 설문에서는 학교 구성원 모두 보통수준으로 답하였으며, 체육시설에 대해서는 불만족스럽다라고 응답하였다. 구내림 개선사항으로는 구내림 보존 및 정비, 체육시설확대, 조깅코스개발, 약수 및 연적지 물 관리 등에 대한 의견이 도출되었다. 구내림의 외부 방문자들에 대한 설문에서는 대부분 50세 이상인 퇴직자와 전업주부가 학교 숲을 찾고 있으며, 방문자들은 주로 오전시간에 80%가 찾아오는 것으로 조사되었고, 혼자 방문하는 경우가 70%를 차지하였다. 방문목적은 건강을 위한 산책 및 약수물 이용을 위하여 방문하는 것으로 나타났으며, 가장 많이 이용하는 구내림 장소는 연적지인 것으로 조사되었다. 구내림에 대한 만족도는 대부분 만족하는 것으로 나타났고, 구내림 개선에 대한 사항에는 체육시설 확대가 가장 많았으며 구내림 훼손방지, 산책로 및 벤치시설증대에 대한 요구가 있었다. This study was carried out to investigate the behavioral pattern of student faculty, administration staff, and visitor by questionnaire about campus forest of Kangwon National University. In the question of the necessity of the campus forest, 97% of students, 100% of faculty and 91% of administration staff agreed with the necessity of the campus forest and 80% of university members use the campus forest. The most frequent site of the campus forest was the Yeon-Juk pond for students and administration staff and was the site behind the art building for faculty. The main reason for use of the campus forest was mostly a walk. About the questions dealt with one's satisfaction regarding the campus forest, most members evaluated the average level, and the facilities of physical training were evaluated if the unsatisfied level. The improvements for the campus forest were suggested about conservation of the campus forest, increasing of physical training facilities and the jogging courses, and managing the mineral water and pond. The visitors using the campus forests were mostly retied people or housewives elder than 50 years old. 80% of visitors visits mostly in the morning and 70% of the visitors comes to the campus forest by oneself. The main reasons for use of the campus forest were a walk and dipping the mineral water. The most frequent location was the Yeon-Juk pond for the visitors. Most visitors were strongly satisfied with using of the campus forest. In the questionnaire about improvements of the campus forest, the visitors suggested the increasing of physical training facilities, conservation of the campus forest, and establishing more walking roads and beaches.

급성 진행형 국소 신경학적 증상을 보인 쯔쯔가무시병 1예에서 고용량 스테로이드의 사용

최현철,위성헌,이소연,김상일,박성경,정연주,정소령,전정수,강문원 대한감염학회 2002 감염 Vol.34 No.6

서울거주 일반성인의 수면양상의 관한 연구

최소현,서광윤,김 인 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.2

The purpose of this study was to investigate the sleep patterns of the general adult population. The subjects of this study were 1,049 general adults in Soul and this study was performed from March 1990 to July 1990. The results were as follows ; 1) The mean value for sleep length was 6.42±1.41 hours and there were no significant sex difference. In the above 51-year-old age group, sleep length was significantly shorter compared with the other two age groups(p<0.05), 202(19.3%) reported sleeping more than 8 hours and 847(80.7%) reported sleeping less than 8 hours. 2) The mean value for sleep latency was 23.40±23.03 minutes and there were no significant sex difference. In the above 51-year-old age group, sleep latency was longer than the other two age groups(p<0.05). The mean value for sleep latency in the group of respondents who complained difficulty falling asleep(DFA) was 26.85±25.51 minutes and in the group of respondents who did not complain difficulty falling asleep less than 8 hours. 2) The mean value for sleep latency was 23.40±23.03 minutes and there were no significant sex difference. In the above 51-year-old age group, sleep latency was longer than the other two age groups(p<0.05). The mean value for sleep latency in the group of respondents who complained difficulty falling asleep(DFA) was 26.85±5.51 minutes and in the group of respondents who did not complain difficulty falling asleep was 17.64±24.31minutes. The former was significantly longer than the latter(p<0.000l). 3) The time of awakening was earlier than the other two age groups (p<0.05). The mean value for the time of awakening in the group of respondents who complained early morning awakening was 5.41±1.20hrs. and in the group of respondents who did not complain early morning awakening was 5.52±1.02hrs. There were no significant difference bet ween the two groups. 4) 654(62.3 % ) complained difficulty falling asleep(DFA), 759(72.4%) complained broken sleep (BS), and 422(40.2%) complained early morning awakening(EMA). Broken sleep was more common in females than males (p<0.05) and early morning awakening was more common in above 51-year-old age group(p<0.05). 5) 301(28.7%) had a nap and the frequency of nap was significantly more in the above 51-year-old age group (p<0.000l). 6) The number of subjects who experienced daytime drowsness were significantly more in the 18-30-year-old age group than the other two age groups(P<0.000l). Snoring was significantly more frequent in the above 51-year-old age group (p<C0.005) and in the males than the females(p<0.005). 7) Of the total sample, 428(40.8 %) reported nightmares. 399(38.0%) reported sleeptalking. 178(17.0%) reported bruxism. and 24(2.3%) reported sleepwalking. Nightmares was more common in the 18-30-year-old age group(p<0.0l).

주요우울증 환자의 Interleukin-1β, -2, -6 생산성과 혈청 농도의 변화 및 시상하부-뇌하수체-부신피질축 기능의 상관성

최소현,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.3

주요우울증의 면역기능 이상과 HPA axis 사이의 관계를 규명하고자 본 연구를 시행하였다. DSM-Ⅲ-R 진단기준에 의해 주요우울증으로 진단된 16명의 여러 환자를 대상군으로 하고 대상군과 연령을 일치시킨 16명의 여자를 대조군으로 선택하였다. Basal cortisol 농도와 IL-1β, IL-2, IL-6 생산성 및 형청농도를 두군에서 각각 측정하여 비교하였다. 또한 주요우울증군에서 IL-1β, IL-2, IL-6 생산성 및 혈청농도와 basal cortisol 농도, post-DST cortisol 간의 상관관계를 알아보았다. 그 결과는 다음과 같다. 1) 주요우울증군의 basal cortisol 농도14.4±4.6㎍g/dl, 대조군은 10.1±5.2㎍/dl로 주요우울증군이 정상대조군보다 유의하게 높았다(t=2.47 : df=30, p〈0.05). 2) IL-2 생산성은 주요우울증군 1747.3±387.9pg/ml, 정상대조군 2520.2±884.1pg/ml로 주요우울증군이 대조군보다 유의하게 낮았다(t=-3.08 : df=30, p〈0.05). IL-1β 생산성과 IL-6 생산성은 양군간에 유의한 차이가 없었다. 3) IL-2 혈청농도는 주요우울증군 16명중 12명(75%), 정상대조군 16명중 10명(62.5%)에서 측정이 가능하였으며, 주요우울증군 109.3±22.8pg/ml, 정상대조군 143.8±72.0pg/ml,로 양군간에 유의한 차이는 없었다. IL-1β혈청농도는 양군에서 각각 3명씩 검출이 가능하였고, IL-6 혈청농도는 양군 모두에서 검출되지 않았다. 4) 주요우울증군에서 IL-2 생산성과 post-DST cortisol 농도사이에 유의한 역상관관계가 있었으며 (r=-0.89), IL-2 혈청농도와 post-DST cortisol 농도사이에 유의한 역상관관계가 있었다(r=-0.97). 주요우울증군에서 IL-2 혈청농도와 basal cortisol 농도사이에 유의한 역상관관계가 있었으나(r=-0.65), IL-2 생산성과 basal cortisol 농도사이에 유의한 상관관계는 없었다. 주요우울증군에서 IL-1β, IL-6 생산성과 basal cortisol 농도, post-DST cortisol 농도 사이에 유의한 상관관계는 없었다. 이러한 결과들은 주요우울증에서 면역기능이 저하되어있고 이러한 면역기능저하에 HPA axis 활성화가 관련되어 있다는 가설에 부가적인 자료로 제시될 수 있겠다. The present study was carried out in order to investigate the relationship between immune function and the activity of hypothalamic-pituitary-adrenal(HPA) axis is patients with major depression. The subjects were 16 female major depressives and 16 female healthy controls. We measured mitogen-induced production of IL-1β, IL-2, IL-6 and serum level of IL-1β, IL-2, IL-6 and basal plasma cortisol levels at 8 : 00 a.m. We measured post-DST(dexamethasone suppression test) cortisol levels in 16 major depressive. The results were as follows : 1) Basal cortisol level was significantly higher in the patients with major depression than in the healthy controls(14.4±4.6㎍g/dl, 10.1±5.2㎍/dl, respectively, p〈0.05). 2) IL-2 production was significantly lower in the patients with major depression than in the healthy controls(1747.3±387.9pg/ml, 2520.2±884.1pg/ml, respectively, p〈0.05). There were no significant differences in IL-1β and IL-6 production between the patients with major depression and the healthy controls. 3) Serum level of IL-2 was detectable in 12 to 16 patients with major depression and in 10 of 16 healthy controls. There was no significant difference in serum level of IL-2 between two groups. Serum level of IL-1β was detectable in 3 of 16 patients with major depression and of 16 healthy controls. We could not detect serum level of IL-6 in both groups. 4) There was significant negative correlation between IL-2 production and post-DST cortisol level(r=-0.89) in the 16 patients with major depression. There was significant negative correlation between serum level IL-2 and post-DST cortisol level(r=-0.97) in the 12 patients with major depression. There was significant negative correlation between serum level IL-2 and basal cortisol level(r=-0.65) in the 12 patients with major depression. But there was no significant correlation between IL-2 production and basal cortisol level in the 16 patients with major depression. These finding suggest that immune function is decreased in major depression and the decreased immune function is highly related to the hyperactivity of the HPA axis.

Starter 및 멸치액젓 첨가가 김치양념 및 겉절이 김치의 품질에 미치는 영향

최택권,박소희,유진현,임호수,조재선,황성연 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.2

양념에 Leu. mesenteroides, Lac plantarum의 젖산균과 멸치액젓 첨가가 김치 양념의 대장균군 제어, 속성촉진여부 및 관능적 특성에 미치는 영향을 조사하였다. Leu. mesenteroides과 젓갈을 첨가한 경우 초기 숙성이 촉진되나 후기 산패는 완만하였으며 Lac. plantarum의 첨가시에는 초기 숙성에는 큰 차이가 없고 후기 산패가 현저하게 진행되었다. 대장균군은 산도 0.80% 이상에서 사멸되었는데 leu. mesenteroides의 경우가 숙성이 촉진되어 보다 신속히 사멸시켰다. 총균수와 젖산균수는 초기에 starter 첨가구는 10^6CFU/mL 정도인데 반해서 starter를 첨가하지 않은 않은 처리구에서 총균수는 10^6CFU/mL , 젖산균수가 10^4CFU/mL 이었고 숙성 후에는 첨가균이 높은 수준을 유지하였다. 하지만 대체적으로 스타터의 첨가가 숙성 및 대장균에 크게 영향을 주지 못하는 경향으로 동일하게 나왔다. 관능적 특성에서는 Leu. mesenteroides 첨가구가 Lac. plantarum의 경우보다 우수하였다. 한편, 시판 겉절이 김치는 산도가 0.09~0.18%, ph 5.46~5.89, 총균수 2.2×10^6~3.1×10^7CFU/mL, 젖산균수는 2.1×10^4~6.6×10^5CFU/mL, 대장균수는 1.8×10^4~7.1×10^5CFU/mL, 염분 1.89%~2.51%으로 스타터 첨가 겉절이에 비해 ph와 염도를 제외한 품질 특성에서 전반적으로 낮은 값을 나타냈다. This study was performed in order to investigate the effect of Leuconostoc mesenteroides, Lactobacillus plantarum and salt fermented anchovy extracts on Kimchi sauce. The sauce was fermented more rapidly by addition of Leu. mesenteroids or salt fermented anchovy extracts on the early fermentation stage than the control, but it was fermented slowly on the late fermentation stage. When L. plantarum was added to the Kimchi on the early fermentation stage, the acidity of Kimchi didn't show a significant difference from the control, but acidity was remarkably increased on the late stage. Coliform group was disappeared when acidity of sauce was higher than 0.8% during fermentation. It was controlled by Leu. mesenteroides but not by L. plantarum. Total count and lactic acid bacterial count of the sauces with starter were 6.30×10^6~1.0×10^7 CFU/mL and 1.04~2.04×10^6 CFU/mL, respectively, but those of the control sauce were 10^6 CFU/mL and 10^4 CFU/mL, respectively. Those count of the sauce with starter were higher than those of the control sauce on the later stage of fermentation. Organoleptic quality of the sauce with Leu. mesenteroides was superior to that with L. plantarum.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

임상검체에서 분리된 그람 음성 세균들에 대한 Isepamicin의 시험관내 항균력

권현희,박수진,소민욱,박현구,최성호,김미나,최상호,정진용,우준희,김양수 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

Background : The increasing incidence of multidrug-resistant gram-negative bacteria causing nosocomial infections is an important clinical problem. Isepamicin is a recently developed aminoglycoside which has been known to have potent activity against gram-negative organisms. We evaluated the in vitro activities of isepamicin and other aminoglycosides against a large number of gram-negative organisms. Materials and Methods : We tested the in vitro antimicrobial activities of isepamicin, amikacin, gentamicin, and tobramycin against 566 gram-negative organisms collected between January 2006 and June 2006 in Asan Medical Center. Minimal inhibitory concentrations (MICs) were determined and interpreted according to the recommendations of Clinical and Laboratory Standard Institute (CLSI). The breakpoint MIC used for interpretation of isepamicin was MIC≤16 ㎍/mL as susceptible, 32 ㎍/mL as intermediate, and 64 ㎍/mL as resistant. Results : The MIC_(50)/MIC_(90) of isepamicln for Escherichia coli, Klebsiella pneumon/ae, Pseudomonas aeruginosa, Acinetobacter baumannii, and Enterobacter cloacae were 1/2, 0.5/>128, 4/16, 16/>128, and 1/2 ㎍/mL, respectively. The susceptibilities for E. coli, K. pneumoniae, P. aeruginosa, A. baumannii, and E. cloacae were 100%, 86.4%, 89.7%, 50.0%, and 96.6%, respectively. For E. coli, K. pneumoniae, P. aeruginosa, and E. cloacae, isepamicln had better in vitro activities than gentamicin and tobromycin, and had similar activities with amlkacin. For A. baumanii, all four tested aminoglycosides had similar in vitro activities. Conclusion : Isepamicin had excellent in vitro activities against gram-negative organisms, except A. baumanii. The overall in vitro activities were similar with amikacin. 목적 : 그람 음성 세균은 다양한 지역사회 및 병원 획득 감염의 원인균이다. 최근에는 병원 감염을 일으키는 그람 음성 세균에서 항균제 내성균의 출현과 확산이 큰 문제가 되고 있다. Isepamicin은 최근에 소개된 gentamicin B의 유도체로 외국에서의 연구에 의하면 그람 음성 세균들에 대한 항균력이 우수함이 보고 된 바 있다. 본 연구에서는 우리나라의 임상 균주들을 대상으로 그람 음성세균에서 isepamicin의 시험관내 항균력을 기존의 아미노배당체 제재들과 비교하여 평가하고자 하였다. 재료 및 방법 : 2006년 1월에서 6월 사이에 서울아산병원 환자에서 분리된 Escherichia coli 113 균주, Klebsiella pneumoniae 110 균주, Pseudomonas aeruginosa 117 균주, Acinetobacter baumannii 108 균주, 그리고 Enterobacter cloacae 118 균주를 대상으로 isepaimcin, amikacin, gentamicin, tobramycin에 대한 최소억제농도(minimal inhibitory concentration, MIC)를 한천 희석법(Mueller-Hinton Agar)을 이용하여 측정하였다. 감수성여부는 CLSI 기준에 따랐으며, isepamicin은 MIC≤16㎍/mL를 감수성, MIC=32 ㎍/mL를 중등도 내성, MIC≥64 ㎍/mL를 내성으로 판정하였다. 결과 : Isepamicm의 E. coli, K. pneumoniae, P. aeruginosa, A baumannii, E. cloacae에 대한 MIC_(50)/MIC_(90)은 각각 1/2, 0.5/>128, 4/16, 16/>128, 1/2 ㎍/mL였고, 항균제 감수성률은 차례대로 100%, 86.4%, 89.7%, 50.0%, 96.6%였다. 기존 아미노배당체 제재들과 비교하면 E. coli, K. pneumoniae, P. aeruginosa, E. cloacae에서는 amikacin과 비슷한 감수성률을 보였고, gentamicin과 tobramycin보다 높은 감수성률을 보였다. A baumannii의 경우 isepamicin의 감수성률이 50.0%로 비교적 낮았지만 amikacin, gentamicin, tobramycin도 각각 50.0%, 46.3%, 47.2%의 감수성률을 나타내어 4가지 항균제가 모두 비슷한 항균력을 보였다. 결론 : Isepamicin은 그람 음성 세균들에 있어 기존의 아미노배당체 제재들과 비교했을 때 amikacin과는 동등한 시험관내 항균력을 보이고 gentamicin, tobramycin보다 뛰어난 시험관내 항균력을 보여 사용이 유망한 아미노배당체 중 하나라 생각된다.