人 諸臟器 Alkaline Phosphatase Isoenzyme의 電氣泳動 및 定量

金在植,金京善,金重明,金榮泰,蔣卓仙,李鉉基 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

著者들은 家兎와 人體로부터 採取한 血淸과 諸臟器組織을 使用하여 ALPI 電氣泳動像을 觀察하고 生化學的 定量法을 利用하여 ALP의 活性度를 測定試圖하였으며 臟器特異性 沮害因子에 對한 影響을 觀察하였다. 家兎 血淸 및 諸臟器組織 ALPI電氣泳動에서 血淸은 ±의 活性度를 가진 α2와 α2/β分劃을 나타내었으며 肝組纖은 血淸보다 빠른 移動度를 가진 ??의 α_1과 α_2分劃을 나타내었다. 그리고 肝, 骨, 腎 淋巴腺, 脾臟 및 腸組織에서는 顯著하게 높은 活性度를 나타내는 各各 서로 相異한 分劃을 볼 수 있었으며 人體諸 臟器와大體로 類似하였으나 家兎 血淸의 ALP는 사람에서 와는 다르게 肝組織의 分劃과 一致하지 않고 오히려 骨組織과 더 잘 一致되는 것 같았다. ALPI 活性度에 있어서 家兎의 諸臟器는 生化學的 定量法으로 腎 ALP의 活性度가 49.5B.L. units로 다른 臟器組織에 비하여 顯著하게 높았으며, 그 다음은 脾臟, 脊椎骨, 肝, 肺淋巴腺 및 脾 順으로 높은 活性度를 나타내었으며 人體에 있어서는 胎盤, 骨, 小賜, 脾臟, 肝 및 腎臟의 順으로 높았다. 臟器 特異性 沮害因子에 對한 影響에 있어서 胎盤分劃은 特有하게 耐熱性을 나타내었고 보다 urea低抗性이며 肝分劃은 neuraminidase에 對하여 移動度의 遲延을 招來하였다. The studies upon cellulose acetate microzone electrophoretic patterns, quantitative estimation and effects of organspecific inhibitor on ALP isoenzyme of rabbit and human sera and various organ tissues were performed. On electrophoretic pattern the tissue ALP from the liver bone kidney lymph node spleen and intestine showed the characteristic fraction of high activities. The electrophoretic mobility of rabbit serum ALP was not correspond to the liver ALP as observed in human serum and it appeared to be rather corresponded to the bone ALP, which seemed to be the origin of ALP in serum of rabbit. In the quantitative measurement of tissue ALP activity the kidney ALP showed the highest level and the activity of the skeletal muscle ALP was the lowest(1.5 B. L. units), while higher activities were observable in placenta, bone, intestine, spleen, liver and kidney in order. On the effect of organ specific physico chemical agents the placental ALPI was highly stable to the heat and relatively resistant to urea while other organ ALPI were on the contrary. The neuraminidase seemed to effect to the motility of liver fraction.

플레이트거더의 전단강도

최취경,김태영,서성연,김정석,김상섭,김규석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

In the design of plate girder web panels, it is required to evaluate accurately the elastic buckling strength under pure shear. Whether or not the post-buckling strength is accounted for. Currently. Elastic shear buckling coefficients of web panels stiffened by transverse intermediate stiffeners are determined by assuming conservatively that web panels are simply supported at the juncture between the flange and the web. Although the notion of the real boundary condition at the juncture of the web and flanges to be somewhere between simple and fixed has been recognized from early days, the boundary condition has been conservatively assumed. Mainly due to lack of means to evaluate it in a rational manner. In this paper. A series of numerical analyses based on a three-dimensional finite element modeling is carried out to investigate the effects of the geometric parameters on the boundary conditions at the juncture between the flange and the web.

151종 생약제 추출물이 B형 간염 바이러스 표면 항원(HBsAg)과 항체(HBsAb)의 결합반응 그리고 HBV DNA polymerase 활성에 미치는 효과 : 한국산 각종 생약제를 이용한 B형 간염치료제의 개발

정태호,김정철,김문규,이인선,채성철,김승래,정준모,이인수,김승호,함경수 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

세계인구 43억명 중 B형 간염 바이러스 보균자는 2억 1천 5백만명이며 이중 78%인 1억 6천만명 이상이 아시아 대륙에 집중되어 있다. 한국도 B형 바이러스 간염의 침윤지대이다. 본 연구는 B형 바이러스 보균자 혹은 만성간염을 치료할 수 있는 생약제를 탐색할 목적의 일환으로 한국에서 시판하는 생약제 151종을 구입하여 증류수로 추출하고 여과한 후 냉동건조하여 HBs 항원에 대한 결합능, HBV DNA polymerase억제능, tumor necrosis factor(TNF) 유리에 미치는 영향, 그리고 galactosamine 유발 간염에 대한간장보호 작용을 조사하였다. 151종의 생약제중 0.16㎍/㎖ 농도에서 간염 vaccine 내에 함유된 HBs 항원과 결합하는 생약제는 지유, 가자, 복분자, 대황, 정향, 빈량, 산수유, 소목, 마황, 쇄양, 후박, 목과, 양강, 오약, 산사, 목단피 등 16종이었다. 이들 생약제중 대황(Rheum palmatum L. 大黃), 가자(Terminalia chebula R. 訶子), 지유(Sanguisorba officinalis L. 地楡), 복분자(Rubus coreanus M. 覆盆子)는 HBV DNA polymerase 활성도를 비교적 강하게 억제하였으며 빈량(Areca catechu L. 빈랑), 정향(Eugenia caryophyllata T. 丁香), 목과(Chaenomeles japonica L. 木瓜), 산수유(Cornus officinalis S. 山茱萸)는 약하게나마 HBV DNA polymerase 활성을 억제하였다. 또한 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 대상으로 TNF 유리에 미치는 영향을 조사한 결과 소목을 제외한 8종의 생약제 모두가 대식세포를 자극하여 TNF를 유리시켰다. 그리고 장차 임상에 사용함을 전제로 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 galactosamine 유발 간염(백서)에 투여하여 간기능 검사에 미치는 효과를 조사하였으나 간기능 검사상으로는 특별한 보호작용을 나타내지 못하였으며 생약제 단독 투여로서는 간기능에 대하여 나쁜 영향을 주지 않았다. Hepatitis B virus infection is major cause of acute and chrome hepatitis, cirrhosis of liver, and hepatocellular carcinoma. There are over 200 million carriers of hepatitis B virus in the world, nearly 80 % of whom live in the South East Asia, Korea is also highly endemic area of hepatitis B virus infection. This study was performed to evaluate the effect of extracts of traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBs Ag, inhibition of HBV DNA polymerase, release of tumor necrosis factor (TNF), and protection against galactosamine induced liver damage. Among 151 herbs, 16 herbs (Sanguisorba officinalis L., Terminalia chebula R., rubus coreanus M., Rheum palmatum L., Eugenia caryophyllata T., Areca catechu L., Cornus officinalis S., Caesalpinia sappan L, Ephedra sinica S., Cynomorium songaricum R., Machilus thunbergii S., Chaenomeles japonica L., Alpinia officinarum H., Lindera strychnifolia V., Crataegus pinnatifida B., Paeonia suffruticasa A. ) had binding capability to HBs Ag which contained in the commercial hepatitis vaccine, at 16 ug/㎖ of concentratioa Among the above 16 herbs, Rheum palmatum L., Terminalia chebula R., Sanguisorba officinalis L., Rubus coreanus M. significantly inhibited the HBV DNA polymerase activity, and Areca catechu L., Eugenia caryophyllata T., Ephedra sinica S., Caesalpinia sappan L., Chaenomeles Japonica L., Cornus officinalis S. also inibited the HBV DNA polymerase activity but less effective. All of the herbs, except Caesalpinia sappan L., which inhibited the HBV DNA polymerase activity, actively stimulated the mononuclear cells to release tumor necrosis factor(TNF) in whole blood culture system. The herbs which inhibited the HBV DNA polymerase activity, did not show any protective effect in galactosamine-induced hepatitis in rats, but there was no direct toxic effect when herbs were administered alone.

우관상동맥 개구부 완전 폐쇄가 동반된 대동맥 판상부 협착증 1례

임상현,김종진,김희열,진승원,전희경,노태호,채장성,김재형,홍순조,최규보,윤정섭,김치경 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

배뇨장애(排尿障碍)에 대한 침구치료(鍼灸治療)의 연구동향(硏究動向)

김경태,고영진,김용석,김창환,Kim, Kyung-tai,Ko, Young-jin,Kim, Yong-suk,Kim, Chang-hwan 대한침구의학회 2005 대한침구의학회지 Vol.22 No.3

Objective : The aim of this study was to rivew systemically literature and clinical trials in the treatment of urinary incontinence or lower urinary tract syndrome(LUTS). Methods : Computerized literature searches were carried out on two electronic database, and computerized searching on some korea oriental medicine journals in library of Kyung-Hee Medical center. Results : 1. Three reports of review study, six reports of experimental study and fourteen reports of clinical trials were collected and reviewed. Three reports of review study were all printed in the korea oriental medicine journal. From 2000, researches and studies have been increased in quantity and improved in quality. 2. Urinary disturbance include variable symptoms of lower urinary tract symptoms, urinary incontinence, in theaspect of Oriental medicine these symptoms are anurin, dysuria, urinary incontinence, nochumal enuresis, uracratia and so on. 3. Roughly physiological procedure of Acupuncture in Treatment of Urianry Disturbance may be that effect of acupuncture stimulation for parasympathetic nerve, sleep-arousal system in cerebrum, pontine/spinal urination center and pudendal/pelvic nerve affect bladder in expansion of bladder capacity, inhibition of urinary contraction and affection in periurethral muscle by continuous excitement of spinal annular circuit and synapse of neuron. 4. Clinical result for acupuncture treatment in urinary disturbance is summarized that acupuncture treatment in urianation disturbance of Neurogenic Bladder, Incontinence, Cycitis, Nocturnal Enuresis, Prostatitis/Pelvic Pain Syndrom and so on is significant clinical trials and technique. Conclusion : Hereafter, in the old age society these variable urinary disturbance patients are increased and desire of treatment may be also increased. So study of various and formal treatment and tecnnique is needed.

土壤에서 分離된 抗酸菌의 分類, 病原性 및 怪癖性에 關한 硏究

金聖光,崔大卿,柳駿 최신의학사 1975 最新醫學 Vol.18 No.8

Acid-fast organisms were isolated from 120 soil samples collected at four villages in the country, - Tests were conducted to determine the biological and biochemical characteristics and the fastidiousness of the isolated mycobacteria. The pathogenicity of the acid-fast organisms isolated from soil were compared with the pathogenicity of reference strains. The results are summarized as follows: 1. Of the 120 samples cultured, 37(30.8%) yielded atypical mycobacteria. 2. Out of the 37 strains isolated 7 strains would not grow in subculture showing their fastidiousness. Although these seven mycobacterial strains grew well in the primary isolation, they did . not grow in subculture under the same conditions as in their primary isolation, nor did they grow on ordinary laboratory culture media. 3. The isolated strains, when classified by Runyon groups, consisted of 5 strains (16.6%) of scotochromogens, 3 strains (10.0%) of non-photochromogens and 22 strains (73.4%) of rapid growers. 4. According to the biochemical characteristics, the 5 strains of scotochromogen could be subdivided into 2 groups, the 3 strains of non-photochromogen into 3 groups and the 22strains of rapid grower into 6 groups. 5. Acid-fast organisms were observed in liver and spleen of mice inoculated intraperitoneally with three of the strains of rapid grower isolated from soil and in mice inoculated with the reference strain, M. fortuitum, by 3 weeks after inoculation, but no acid-fast organisms were observed in the organs of the mice inoculated with the isolated scotochromogens and non-photochro mogens by 4 weeks. 6. In the histopathological examinations, early granulomatous lesions with acid-fast organisms were observed only in the liver tissue. The fastidiousness of 7 strains of mycobacteria from soil were very similar to the fastidiousness of M. leprae which is still unculturable although there have often been claims to have grown the organisms in primary isolation. Further study on the mycobacterial fastidiousness is needed in the future.

리그닌을 분해하는 Streptomyces lavendulas SA-14에 의한 염료의 탈색

김태전,윤경하 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.1

This study was undertaken to investigate the activity of ligninolytic enzymes and the decolorization capability of some dyes with streptomyces lavendulas SA-14 isolated from soil and identified. Most ligninolytic enzymes exhibited the maximal activity when the strain was grown in basal medium for 6 days. Activities of LiP 2 and VAO among these enzymes exhibited 14.25 and 11.23n㏖/㎎ protein respectively. Decolorization rate of amaranth, orange Ⅱ, tropaeolin O and poly R-478 was more than 72% on 10 days incubation while that of crystal violet and malachite green was 6.7% and 6.6% respectively.

자기공명영상을 이용한 만성 족관절통의 원인 분석

김현철,이경태,유재원,이상홍,선재명 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Purpose : Chronic ankle pain is one of the most popular problems with orthopedic clinicians, but it has complex etiologic factors in diagnosis. Recently, magnetic resornance imaging has used as common diagnostic modality in ligaments and tendon problems of ankle sprain. The purpose of this article is to determine the appearances and frequency of chronic ankle sprain by MRI, and to determine the value of MRI as a diagnostic tool. Materials and Methods : We have analysed MRI findings of 84 chronic sprained ankles over 6 months duration from September 1995 to January 1997. All cases had been past history of ankle injury. Among them, sports players ie, soccer, basket ball, competitive runner and ballet dancer are 51 cases( 60.7% ). Average age of patients is 27.06( 13 to 54 ) years old and sex ratio is 23:5. For the confirmative diagnosis, We adopted variable methods, ie, ultrasonogram, bone scan, CT scan, arthroscopic examination and surgical intervention. Results : The Etiologic factors of chronic ankle sprain by MRI includes 39 Lateral ankle instability due to Anterior Talofibular and Calcaneofibular ligamentous tear, 18 Peroneal tendon insufficiency ie, synovitis, longitudinal tear, subluxation and abnormal tendon insertion, 15 Osteochondral fracture of talus or tibia, 10 Anterior Impingement syndrome due to anterior bony spur, 9 Deltoid tear, 6 Os subfibulare, 4 Distal tibiofibular ligament abnormality, 3 Os subtibiale, 2 Ankle synovitis, 2 Intraarticular loose body. Conclusion : MRI study employed in hazy chronic ankle sprain is useful diagnostic modality. But, Diagnostic result using MRI was not correlated the clinical prognosis, and proper physical examination is important in the point of technical aspect to get the exact dissecting angle of MRI.

서비스 주체의 상호 협력 관계를 제공하는 멀티미디어 Qos 프레임워크

김태윤,장경아,유항재 高麗大學校附設 컴퓨터科學技術硏究所 1999 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.2 No.-

인터넷 사용자의 급속한 증가와 전자 상거래의 발달로 신뢰성, 보안성, 확장성, 유연성 등의 기본적인 요구 사항 뿐 아니라 멀티미디어 응용 서비스에 대한 필요성이 제기되고 있다. 멀티미디어 컴퓨팅과 커뮤니케이션은 네트워크, 통신망, 오퍼레이팅 시스템에서 다양한 메커니즘을 필요로 한다. 대규모 분산 환경에서 QoS는 Video-on-Demand, News-On-Demand 등의 단일 매체에 대한 서비스와는 다르게 응용 매체에 대한 우선 순위 부여 및 상호 협력 구조적 서비스 및 MPEG 비디오 서비스와 같은 멀티미디어 응용 서비스에 대한 지원 능력을 요구하고 있다. 본 논문에서는 인터넷을 기반으로 대단위 분산 시스템 환경에서 QoS를 지원하기 위한 통합적 QoS 프레임워크로 E-QoS 모델을 설계하였으며, 향후 이를 바탕으로 한 세부 메커니즘의 설계 방향을 제시하였다. The huge Internet and electronic commerce shall be provided not only basic requirements of reliability, security extendibility, flexibility but also adaptive application service of multimedia data. There are required a lot of mechanism for network, operating system in multimedia computing and communication. In large distributed computing, QoS shall be provided a priority allocation service and an interactive infrastructure service as like not single media service of Video-on-Demand, and News-on-Demand but multimedia application service of MPEG video service. In this paper, supporting large distributed computing based on Internet, we propose a E-QoS Model as Integrated QoS Framework, and propose a direction of design for specific mechanism in the future works.

Scytalidium acidiphilum이 생산하는 내산, 내열 이눌린아제

金美京,嚴泰鵬 全北大學校 1994 論文集 Vol.38 No.-

The intracellular exoinulinase from Scytalidium acidophilum was purified to the specific activity of about 1,000 U/㎎ by ammonium sulfate precipitation, DEAE-Sepharose CL-6B, CM-Sephadex C-50, and HPLC gel filtration column. The maximum activity of the enzyme was observed between pH 3-3.5, which was the most acidic range among the optimum pHs of inulinases so far reported. Also, the enzyme could maintain 85% of its original activity after 6 h of incubation at 65℃, which had the industrial potential for inulin hydrolysis.