人 諸臟器 Alkaline Phosphatase Isoenzyme의 電氣泳動 및 定量

金在植,金京善,金重明,金榮泰,蔣卓仙,李鉉基 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

著者들은 家兎와 人體로부터 採取한 血淸과 諸臟器組織을 使用하여 ALPI 電氣泳動像을 觀察하고 生化學的 定量法을 利用하여 ALP의 活性度를 測定試圖하였으며 臟器特異性 沮害因子에 對한 影響을 觀察하였다. 家兎 血淸 및 諸臟器組織 ALPI電氣泳動에서 血淸은 ±의 活性度를 가진 α2와 α2/β分劃을 나타내었으며 肝組纖은 血淸보다 빠른 移動度를 가진 ??의 α_1과 α_2分劃을 나타내었다. 그리고 肝, 骨, 腎 淋巴腺, 脾臟 및 腸組織에서는 顯著하게 높은 活性度를 나타내는 各各 서로 相異한 分劃을 볼 수 있었으며 人體諸 臟器와大體로 類似하였으나 家兎 血淸의 ALP는 사람에서 와는 다르게 肝組織의 分劃과 一致하지 않고 오히려 骨組織과 더 잘 一致되는 것 같았다. ALPI 活性度에 있어서 家兎의 諸臟器는 生化學的 定量法으로 腎 ALP의 活性度가 49.5B.L. units로 다른 臟器組織에 비하여 顯著하게 높았으며, 그 다음은 脾臟, 脊椎骨, 肝, 肺淋巴腺 및 脾 順으로 높은 活性度를 나타내었으며 人體에 있어서는 胎盤, 骨, 小賜, 脾臟, 肝 및 腎臟의 順으로 높았다. 臟器 特異性 沮害因子에 對한 影響에 있어서 胎盤分劃은 特有하게 耐熱性을 나타내었고 보다 urea低抗性이며 肝分劃은 neuraminidase에 對하여 移動度의 遲延을 招來하였다. The studies upon cellulose acetate microzone electrophoretic patterns, quantitative estimation and effects of organspecific inhibitor on ALP isoenzyme of rabbit and human sera and various organ tissues were performed. On electrophoretic pattern the tissue ALP from the liver bone kidney lymph node spleen and intestine showed the characteristic fraction of high activities. The electrophoretic mobility of rabbit serum ALP was not correspond to the liver ALP as observed in human serum and it appeared to be rather corresponded to the bone ALP, which seemed to be the origin of ALP in serum of rabbit. In the quantitative measurement of tissue ALP activity the kidney ALP showed the highest level and the activity of the skeletal muscle ALP was the lowest(1.5 B. L. units), while higher activities were observable in placenta, bone, intestine, spleen, liver and kidney in order. On the effect of organ specific physico chemical agents the placental ALPI was highly stable to the heat and relatively resistant to urea while other organ ALPI were on the contrary. The neuraminidase seemed to effect to the motility of liver fraction.

플레이트거더의 전단강도

최취경,김태영,서성연,김정석,김상섭,김규석 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

In the design of plate girder web panels, it is required to evaluate accurately the elastic buckling strength under pure shear. Whether or not the post-buckling strength is accounted for. Currently. Elastic shear buckling coefficients of web panels stiffened by transverse intermediate stiffeners are determined by assuming conservatively that web panels are simply supported at the juncture between the flange and the web. Although the notion of the real boundary condition at the juncture of the web and flanges to be somewhere between simple and fixed has been recognized from early days, the boundary condition has been conservatively assumed. Mainly due to lack of means to evaluate it in a rational manner. In this paper. A series of numerical analyses based on a three-dimensional finite element modeling is carried out to investigate the effects of the geometric parameters on the boundary conditions at the juncture between the flange and the web.

151종 생약제 추출물이 B형 간염 바이러스 표면 항원(HBsAg)과 항체(HBsAb)의 결합반응 그리고 HBV DNA polymerase 활성에 미치는 효과 : 한국산 각종 생약제를 이용한 B형 간염치료제의 개발

정태호,김정철,김문규,이인선,채성철,김승래,정준모,이인수,김승호,함경수 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

세계인구 43억명 중 B형 간염 바이러스 보균자는 2억 1천 5백만명이며 이중 78%인 1억 6천만명 이상이 아시아 대륙에 집중되어 있다. 한국도 B형 바이러스 간염의 침윤지대이다. 본 연구는 B형 바이러스 보균자 혹은 만성간염을 치료할 수 있는 생약제를 탐색할 목적의 일환으로 한국에서 시판하는 생약제 151종을 구입하여 증류수로 추출하고 여과한 후 냉동건조하여 HBs 항원에 대한 결합능, HBV DNA polymerase억제능, tumor necrosis factor(TNF) 유리에 미치는 영향, 그리고 galactosamine 유발 간염에 대한간장보호 작용을 조사하였다. 151종의 생약제중 0.16㎍/㎖ 농도에서 간염 vaccine 내에 함유된 HBs 항원과 결합하는 생약제는 지유, 가자, 복분자, 대황, 정향, 빈량, 산수유, 소목, 마황, 쇄양, 후박, 목과, 양강, 오약, 산사, 목단피 등 16종이었다. 이들 생약제중 대황(Rheum palmatum L. 大黃), 가자(Terminalia chebula R. 訶子), 지유(Sanguisorba officinalis L. 地楡), 복분자(Rubus coreanus M. 覆盆子)는 HBV DNA polymerase 활성도를 비교적 강하게 억제하였으며 빈량(Areca catechu L. 빈랑), 정향(Eugenia caryophyllata T. 丁香), 목과(Chaenomeles japonica L. 木瓜), 산수유(Cornus officinalis S. 山茱萸)는 약하게나마 HBV DNA polymerase 활성을 억제하였다. 또한 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 대상으로 TNF 유리에 미치는 영향을 조사한 결과 소목을 제외한 8종의 생약제 모두가 대식세포를 자극하여 TNF를 유리시켰다. 그리고 장차 임상에 사용함을 전제로 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 galactosamine 유발 간염(백서)에 투여하여 간기능 검사에 미치는 효과를 조사하였으나 간기능 검사상으로는 특별한 보호작용을 나타내지 못하였으며 생약제 단독 투여로서는 간기능에 대하여 나쁜 영향을 주지 않았다. Hepatitis B virus infection is major cause of acute and chrome hepatitis, cirrhosis of liver, and hepatocellular carcinoma. There are over 200 million carriers of hepatitis B virus in the world, nearly 80 % of whom live in the South East Asia, Korea is also highly endemic area of hepatitis B virus infection. This study was performed to evaluate the effect of extracts of traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBs Ag, inhibition of HBV DNA polymerase, release of tumor necrosis factor (TNF), and protection against galactosamine induced liver damage. Among 151 herbs, 16 herbs (Sanguisorba officinalis L., Terminalia chebula R., rubus coreanus M., Rheum palmatum L., Eugenia caryophyllata T., Areca catechu L., Cornus officinalis S., Caesalpinia sappan L, Ephedra sinica S., Cynomorium songaricum R., Machilus thunbergii S., Chaenomeles japonica L., Alpinia officinarum H., Lindera strychnifolia V., Crataegus pinnatifida B., Paeonia suffruticasa A. ) had binding capability to HBs Ag which contained in the commercial hepatitis vaccine, at 16 ug/㎖ of concentratioa Among the above 16 herbs, Rheum palmatum L., Terminalia chebula R., Sanguisorba officinalis L., Rubus coreanus M. significantly inhibited the HBV DNA polymerase activity, and Areca catechu L., Eugenia caryophyllata T., Ephedra sinica S., Caesalpinia sappan L., Chaenomeles Japonica L., Cornus officinalis S. also inibited the HBV DNA polymerase activity but less effective. All of the herbs, except Caesalpinia sappan L., which inhibited the HBV DNA polymerase activity, actively stimulated the mononuclear cells to release tumor necrosis factor(TNF) in whole blood culture system. The herbs which inhibited the HBV DNA polymerase activity, did not show any protective effect in galactosamine-induced hepatitis in rats, but there was no direct toxic effect when herbs were administered alone.

우관상동맥 개구부 완전 폐쇄가 동반된 대동맥 판상부 협착증 1례

임상현,김종진,김희열,진승원,전희경,노태호,채장성,김재형,홍순조,최규보,윤정섭,김치경 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

배뇨장애(排尿障碍)에 대한 침구치료(鍼灸治療)의 연구동향(硏究動向)

김경태,고영진,김용석,김창환,Kim, Kyung-tai,Ko, Young-jin,Kim, Yong-suk,Kim, Chang-hwan 대한침구의학회 2005 대한침구의학회지 Vol.22 No.3

Objective : The aim of this study was to rivew systemically literature and clinical trials in the treatment of urinary incontinence or lower urinary tract syndrome(LUTS). Methods : Computerized literature searches were carried out on two electronic database, and computerized searching on some korea oriental medicine journals in library of Kyung-Hee Medical center. Results : 1. Three reports of review study, six reports of experimental study and fourteen reports of clinical trials were collected and reviewed. Three reports of review study were all printed in the korea oriental medicine journal. From 2000, researches and studies have been increased in quantity and improved in quality. 2. Urinary disturbance include variable symptoms of lower urinary tract symptoms, urinary incontinence, in theaspect of Oriental medicine these symptoms are anurin, dysuria, urinary incontinence, nochumal enuresis, uracratia and so on. 3. Roughly physiological procedure of Acupuncture in Treatment of Urianry Disturbance may be that effect of acupuncture stimulation for parasympathetic nerve, sleep-arousal system in cerebrum, pontine/spinal urination center and pudendal/pelvic nerve affect bladder in expansion of bladder capacity, inhibition of urinary contraction and affection in periurethral muscle by continuous excitement of spinal annular circuit and synapse of neuron. 4. Clinical result for acupuncture treatment in urinary disturbance is summarized that acupuncture treatment in urianation disturbance of Neurogenic Bladder, Incontinence, Cycitis, Nocturnal Enuresis, Prostatitis/Pelvic Pain Syndrom and so on is significant clinical trials and technique. Conclusion : Hereafter, in the old age society these variable urinary disturbance patients are increased and desire of treatment may be also increased. So study of various and formal treatment and tecnnique is needed.

토양에서 분리된 방선균들에 의한 Rimazol Brilliant Blue R(RBBR) 염료의 탈색에 질소원과 pH가 미치는 영향

김태전,김승곤,윤경하 서울保健大學 1997 論文集 Vol.17 No.1

Many workers had reported that the degradation of lignocellulose and lignin by white rot fungi were significantily correlative with the degradation of phenol or PAHs (Polycyclic aromatic hydorcarbons) compounds and the decolorization of a various dyes, specially RBBR (Remazol brilliant blue R) and Poly R-478 dyes. but it were not known distinctly that the degradation of these and the decolorization of these by Actinomycetes were related to the degradation of phenol or PAHs compounds, and author had examined in relationship between the degradation of lignocellulose or lignin and the decolorization of RBBR by Actinomycetes isolated in 67 soil samples collected at 8 areas of Korea from February, 1994 to March, 1995. In the experiment, There were selected two strains identified as Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 which had good correlation between the degradation of lignocellulose or lignin, and it were investigated that the cultural parameters to maximize the decolorization ratios of RBBR by two strains, as the results, Streptomyces halstedii scabies SA1-36 revealed each the highest decolorization ratios with 73.4% in 0.30% glycerin and Streptomyces lavendulas SA2-14, with 74.1% in 0.25% cellobiose at 30℃ in 20 days of culture. There were used their results as basal data in this study that examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR. It were summarized as following that the results examined the effects of nitrogen sources and pH to maximize the decolorization ratios of RBBR by two strains. 1) It were the highest each as 75.7% in casein and 75.7% in yeast extract that the dicolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to nitrogen sources(0.l%). 2) It were the highest each as 76.8% in 0.1% casein and 78.3% in yeast extract that the decolorization ratios of RBBR by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains according to the concentrations of casein and yeast extract. 3) It were the highest each as 76.8% in pH 7.6 and 79.4% in pH 6.6 that the decolorization ratios of RBBR according to pH by Streptomyces halstedii scabies SA1-36 and Streptomyces lavendulas SA2-14 strains. In the above results, we could knowe that it were each different as well as carbon sources that nitrogen sources and pH required between Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2- 14 strains. and organic nitrogens influence more than inorganic nitrogens to decolorize RBBR dye and nitrogen sources and pH influence lower than carbon sources to decolorize RBBR dye. What`s more, we could knowe that it were very important to use newly formuliged optimal basal media maximize decolorization ratios of RBBR dye by Streptomyces halstedii scabies SAl-36 and Streptomyces lavendulas SA2-14 strains.

리그닌을 분해하는 Streptomyces lavendulas SA-14에 의한 염료의 탈색

김태전,윤경하 순천향대학교 기초과학연구소 1998 순천향자연과학연구 논문집 Vol.4 No.1

This study was undertaken to investigate the activity of ligninolytic enzymes and the decolorization capability of some dyes with streptomyces lavendulas SA-14 isolated from soil and identified. Most ligninolytic enzymes exhibited the maximal activity when the strain was grown in basal medium for 6 days. Activities of LiP 2 and VAO among these enzymes exhibited 14.25 and 11.23n㏖/㎎ protein respectively. Decolorization rate of amaranth, orange Ⅱ, tropaeolin O and poly R-478 was more than 72% on 10 days incubation while that of crystal violet and malachite green was 6.7% and 6.6% respectively.

Scytalidium acidiphilum이 생산하는 내산, 내열 이눌린아제

金美京,嚴泰鵬 全北大學校 1994 論文集 Vol.38 No.-

The intracellular exoinulinase from Scytalidium acidophilum was purified to the specific activity of about 1,000 U/㎎ by ammonium sulfate precipitation, DEAE-Sepharose CL-6B, CM-Sephadex C-50, and HPLC gel filtration column. The maximum activity of the enzyme was observed between pH 3-3.5, which was the most acidic range among the optimum pHs of inulinases so far reported. Also, the enzyme could maintain 85% of its original activity after 6 h of incubation at 65℃, which had the industrial potential for inulin hydrolysis.

탄소성지반에서 굴착단계별 토류벽의 거동해석에 관한 연구

김수일,김경태 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.2

A computer program to predict the sequential behavior of earth retaining structures during excavation in elastoplastic soil is developed in this study. Validity of the computer program developed is tested by comparing the computer outputs with field measurement data at an excavation site. Through the validity test, it is found that lateral displacements computed are close to measured data when the Terzaghi method is used to obtain the modulus of lateral subgrade reaction and soil pressures computed using elasto-plastic model concept match well with measured soil pressure distribution.

歸因理論과 精神醫學

金慶植,兪泰赫 서울대학교 의과대학 정신과학교실 1983 精神醫學報 Vol.7 No.3